Inside Stain Kit from Miltenyi Biotec

(1) Therapeutic delivery of albumin-binding siRNA targeting IRS2 to diverse cell types reduces mammary tumor growthMolecular TherapyJanuary 29, 2026Claire E. Tocheny, Julianna E. Buchwald, Christopher D. Dahlke, Hassan H. Fakih, Jennifer S. Morgan, Ashley Summers, Christi A. Silva, Samuel O. Jackson, Ji-Sun Lee, Michael-Anthony Card, et al.Viability Dye (Invitrogen #65–0865-14) and fixed using a formaldehyde-based fixative (Miltenyi Cat# 130–090-477). Samples were run on a Cytek Aurora flow cytometer and analyzed using FlowJo v10 software. Single-stained

(2) Simultaneous secretion of a PD-L1 x 4-1BB bispecific antibody improves antileukemic efficacy of STAb-T cells secreting a CD19-specific T-cell engagerOncoimmunologyDecember 31, 2025Marina Gómez-Rosel, Laura Rubio-Pérez, Ivana Zagorac, Carmen Domínguez-Alonso, Laura Díez-Alonso, Oana Hangiu, Rocío Navarro, Raúl Torres-Ruíz, Sandra Rodríguez-Perales, Francisco J. Blanco, et al.APC-conjugated anti-His mAb. Intracellular bsAbs were detected using the Inside Stain Kit (Cat. No. 130−090−477; Miltenyi Biotec), following the manufacturer’s instructions, and an anti-His APC mAb. Alternatively,

(3) Loss of ADAM15 prevents necroptosis induction by partial RIPK1 degradation due to enhanced TNF-R1 surface expression and basal caspase-8 activationCell Commun SignalDecember 4, 2025S. Braun, K. Knackfuß, T. Ziesmann, L. Mlinzk, A. Goerg, J. Frankenheim, A. Walter, W. Schneider-Brachert, U. Distler, J. Fritschlocated both inside cells and on the cell surface [46], we utilized the inside stain kit and protocol (130–090–477, Miltenyi). 1 × 106 cells were sedimented, washed with PBS (10 min, 300 × g), and resuspended in 250

(4) Circulating tumour cells are a prognostic indicator in advanced high-grade serous ovarian cancer and are associated with platelets and immune cells following disseminationBritish Journal of CancerOctober 10, 2025Mark P. Ward, Faye Lewis, Catherine O’Gorman, Lucy A. Norris, Sarah E. Lochrin, Laura E. Kane, Tanya E. Kelly, Bashir M. Mohamed, Ashitha Ramesh, Roisin O’Connor, et al.paraformaldehyde (Sigma Aldrich, Ireland) followed by incubation with permeabilization buffer Inside Perm (Cat:130-090-477, Inside stain Kit; Miltenyi Biotec, Germany). For metastatic breast cancer patient samples, slides were

(5) Myocardial disarray drives metabolic inefficiency in human cardiomyocytesCommun BiolOctober 9, 2025Charlène Jouve, Andrea Ruiz-Velasco, Erminia Donnarumma, Rémi Le Borgne, Iman Momken, Céline Pereira, Magali Seguret, Eva Vermersch, Elodie Vimont, Ivan Nemazanyy, et al.manufacturer’s protocol. Then, collected cell pellets were fixed and permeabilized using Inside Stain kit (130-090-477 Miltenyi Biotech) for 10 min at room temperature. Cells were incubated with fluorescent labeled antibodies

(6) GMP-Compliant Process for the Manufacturing of an Extracellular Vesicles-Enriched Secretome Product Derived From Cardiovascular Progenitor Cells Suitable for a Phase I Clinical TrialJ Extracell VesiclesAugust 20, 2025Camille Humbert, Chloé Cordier, Iouri Drut, Michele Hamrick, Jacquelyn Wong, Valérie Bellamy, Justine Flaire, Kiranmayee Bakshy, Florent Dingli, Damarys Loew, et al.manufacturer's instructions. Intracellular staining was performed using Inside stain kit (Miltenyi Biotec, #130‐090‐477) according to the manufacturer's instructions. Flow cytometric analyses were performed on day+3 and

(7) Frequency of synaptic antigen-specific CD4+ T cells in dementia is age-dependent but not correlated with cognitive impairmentImmun AgeingJune 19, 2025Julius Hoffmann, Marie-Luise Machule, Jakob Kreye, Laura Stöffler, Péter Körtvelyessy, Maria Buthut, Rosa Rößling, Petra Bacher, Alexander Scheffold, Harald PrüssMiltenyi, 130–109–812). Following cell permeabilization and fixation (Miltenyi inside stain kit, Miltenyi 130–090–477), intracellular epitopes were stained with antibodies targeting CD154 (PE, Miltenyi, 5 C8, 130–113–607,

(8) Distinct HLA Haplotypes Are Associated With an Altered Strength of SARS-CoV-2-Specific T-Cell Responses and Unfavorable Disease CoursesEur J ImmunolApril 21, 2025C. Dörnte, A. Datsi, V. Traska, J. Kostyra, M. Wagner, O. Brauns, C. Lamsfuß, H. Winkels, V. Balz, J. Enczmann, et al.markers, cell fixation was performed using Inside Fix (Inside Stain Kit, Miltenyi Biotec, cat. no. 130‐090‐477), according to the manufacturer's instruction, followed by resuspension in 250 µL/well Inside Perm (Inside Stain Kit

(9) Rod-shaped micropatterning enhances the electrophysiological maturation of cardiomyocytes derived from human induced pluripotent stem cellsStem Cell ReportsOctober 8, 2024Zeina R. Al Sayed, Charlène Jouve, Magali Seguret, Andrea Ruiz-Velasco, Céline Pereira, David-Alexandre Trégouët, Jean-Sébastien HulotBioLegend). The collected cell pellets were then fixed and permeabilized with the Inside Stain kit (130-090-477, Miltenyi Biotec) for 10 min at room temperature. Cells were incubated with either Allophycocyanin (APC)

(10) A comparative study of circulating tumor cell isolation and enumeration technologies in lung cancerMolecular OncologyAugust 6, 2024Volga M Saini, Ezgi Oner, Mark P. Ward, Sinead Hurley, Brian David Henderson, Faye Lewis, Stephen P. Finn, Gerard J. Fitzmaurice, John J. O'Leary, Sharon O'Toole, et al.antibody [V9, Alexa Fluor 546, sc‐6260]) were added to 399 μL of permeabilization buffer Inside Perm (#130‐090‐477, Inside stain Kit; Miltenyi Biotec, Bergisch Gladbach, Germany), along with 3.5 μL of 1 mg·mL−1 Hoechst

(11) A versatile high-throughput assay based on 3D ring-shaped cardiac tissues generated from human induced pluripotent stem cell-derived cardiomyocyteseLifeApril 5, 2024Magali Seguret, Patricia Davidson, Stijn Robben, Charlène Jouve, Celine Pereira, Quitterie Lelong, Lucille Deshayes, Cyril Cerveau, Maël Le Berre, Rita S Rodrigues Ribeiro, et al.Then, collected cell pellets were fixed and permeabilized using Inside Stain kit (Miltenyi Biotech, 130-090-477) at room temperature for 10 min. Cells were incubated with either APC anti-cardiac troponin T (CTNT)

(12) Nicotinamide-Expanded Allogeneic Natural Killer Cells with CD38 Deletion, Expressing an Enhanced CD38 Chimeric Antigen Receptor, Target Multiple Myeloma CellsInt J Mol SciDecember 7, 2023Avishay Edri, Nimrod Ben-Haim, Astar Hailu, Nurit Brycman, Orit Berhani-Zipori, Julia Rifman, Sherri Cohen, Dima Yackoubov, Michael Rosenberg, Ronit Simantov, et al.fixation and permeabilization using an Inside Stain Kit (Miltenyi, Bergisch Gladbach, Germany. cat#130-090-477) according to the manufacturer’s instructions. After permeabilization, intracellular staining was performed

(13) Inhibition of microfold cells ameliorates early pathological phenotypes by modulating microglial functions in Alzheimer's disease mouse modelJournal of neuroinflammationNovember 27, 2023Kim N, Ju IG, Jeon SH, Lee Y, Jung MJ, Gee MS, Cho JS, Inn KS, Garrett-Sinha LA, Oh MS, et al.anti-CD68 Alexa488 (1:50, BioLegend, 137012) were performed with Inside Stain kit (Miltenyi Biotec, 130-090-477) according to the manufacturer’s instructions. After an incubation period of 1 h at 4 °C, fluorescence

(14) Oral Delivery of mRNA Vaccine by Plant-Derived Extracellular Vesicle CarriersCellsJuly 11, 2023Margherita A. C. Pomatto, Chiara Gai, Federica Negro, Lucia Massari, Maria Chiara Deregibus, Francesco Giuseppe De Rosa, Giovanni Camussiacquisition. For intracellular cytokine analysis, cells were previously permeabilized using Inside Stain Kit (130-090-477, Miltenyi, Bologna, Italy). For cell proliferation, splenocytes were stained with CellTrace™ CFSE cell

(15) Streptococcus pneumoniae drives specific and lasting Natural Killer cell memoryPLoS pathogensJuly 1, 2023Camarasa TMN, Torné J, Chevalier C, Rasid O, Hamon MA.IFNγ, cells were permeabilized and stained in buffer from commercial kit (Inside Stain Kit, ref no. 130-090-477, Miltenyi Biotec) for 40 minutes at 4°C. After a final wash suspended in DPBS, sample acquisitions were

(16) Novel CRISPR-Associated Gene-Editing Systems Discovered in Metagenomic Samples Enable Efficient and Specific Genome EngineeringThe CRISPR JournalJune 1, 2023Rebecca C. Lamothe, Meghan D. Storlie, Diego A. Espinosa, Rachel Rudlaff, Patrick Browne, Jason Liu, Andres Rivas, Audra Devoto, Jennifer Oki, Ashcon Khoubyari, et al.Attune NxT Flow Cytometer (ThermoFisher). For staining of intracellular markers, the Inside Stain kit (Miltenyi No. 130-090-477) was used according to the manufacturer's instructions. The following monoclonal antibodies were purchased

(17) Immunotherapy targeting plasma ASM is protective in a mouse model of Alzheimer's diseaseNature communicationsMarch 24, 2023Choi BJ, Park MH, Park KH, Han WH, Yoon HJ, Jung HY, Hong JY, Chowdhury MR, Kim KY, Lee J, et al.anti-IL17 PerCp Cy5.5 (1:100, BD Bioscience, 553142) were performed with Inside Stain Kit (Miltenyi Biotec, 130-090-477) according to the manufacturer’s instructions. Analysis was performed using Attune NxT flow cytometer

(18) Melatonin Activates Anti-Inflammatory Features in Microglia in a Multicellular Context: Evidence from Organotypic Brain Slices and HMC3 CellsBiomoleculesFebruary 16, 2023Merlo S, Caruso GI, Korde DS, Khodorovska A, Humpel C, Sortino MA.and Cell Counts on HMC3 Cells Cells were fixed with InsideFix (Miltenyi, Bologna, Italy; Cat. No. 130-090-477) and incubated with the primary antibody rabbit anti-SIRT1 (1:80; Abcam, Cambridge, United Kingdom;

(19) Chemotherapeutics Used for High-Risk Neuroblastoma Therapy Improve the Efficacy of Anti-GD2 Antibody Dinutuximab Beta in Preclinical Spheroid ModelsCancers (Basel)January 31, 2023Sascha Troschke-Meurer, Maxi Zumpe, Lena Meißner, Nikolai Siebert, Piotr Grabarczyk, Hannes Forkel, Claudia Maletzki, Sander Bekeschus, Holger N. LodeViobility™ 405/452 Fixable Dye (1:100 in 1× PBS, 15 min). Cells were fixed by using Inside Stain Kit (Miltenyi Biotec, 130-090-477) according to manufacturer’s guidelines. A total of 10 µL of Tandem Signal Enhancer was added prior

(20) Impaired activation of plasmacytoid dendritic cells via toll-like receptor 7/9 and STING is mediated by melanoma-derived immunosuppressive cytokines and metabolic driftFrontiers in immunologyJanuary 1, 2023Monti M, Ferrari G, Grosso V, Missale F, Bugatti M, Cancila V, Zini S, Segala A, La Via L, Consoli F, et al.antibodies. Then, pDCs were fixed and permeabilized using the Inside Stain Kit (Miltenyi Biotec; cat. No. 130-090-477) and intracellular staining was performed using the anti-IFN-α (Miltenyi Biotec; cat. No. 130-116-874)

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Inside Stain Kit

Inside Stain Kit from Miltenyi Biotec

Miltenyi Biotec

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Inside Stain Kit from Miltenyi Biotec

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