Grace's Insect Medium, unsupplemented from Thermo Fisher Scientific

Product Specs
ItemGrace's Insect Medium, unsupplemented
CompanyThermo Fisher Scientific
Price $95.00Get Pricing Get Quote for this Product
Catalog Number11595030
ApplicationsGene Expression
Regulatory StatusResearch
Quantity500 mL
TypeMedium

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Thermo Fisher Scientific

168 Third Avenue

Waltham, MA 02451

United States

Phone: 800-678-5599

Company Profile

Website: www.thermofisher.com

(1) Rapid disassembly and Piwi-independent reassembly of Drosophila piRNA cluster heterochromatin following acute heat shockRNAMarch 1, 2026Nicholas P. Rice, Samantha Ho, Zhiping Weng, William E. Theurkauf,Thermo Fisher Scientific,Cat# EN0531 Protease K,, "Grace's Insect Medium, unsupplemented",Thermo Fisher Scientific,Cat# 11595030 Schneider's Drosophila Medium,Thermo Fisher Scientific,Cat# 21720024 Insulin solution from bovine pancreas

(2) Meiotic cohesion requires Sirt1 and preserving its activity in aging oocytes reduces missegregationEMBO ReportsNovember 10, 2025Zihan Meng, Nicholas G Norwitz, Sharon E BickelD1306 "Formaldehyde, 16%",Ted Pella,Cat# 18505 Formamide,Invitrogen,Cat# AM9342 Grace’s Medium,Thermo Fisher,Cat# 11595030 Heptane,Fisher Scientific,Cat# H-350-4 Hoechst 33342,Thermo Fisher,Cat# H3570 Normal donkey serum,Jackson ImmunoResearch

(3) Converging mechanism of UM171 and KBTBD4 neomorphic cancer mutationsNatureFebruary 12, 2025Xiaowen Xie, Olivia Zhang, Megan J. R. Yeo, Ceejay Lee, Ran Tao, Stefan A. Harry, N. Connor Payne, Eunju Nam, Leena Paul, Yiran Li, et al.(catalogue nos. B85502 and A35243, respectively), with Grace insect medium (Thermo Fisher, catalogue no. 11595030) supplemented with 10% FBS (Cytiva) and 1% penicillin-streptomycin (Gibco), and cultured at 26 °C. All

(4) UM171 glues asymmetric CRL3–HDAC1/2 assembly to degrade CoREST corepressorsNatureFebruary 12, 2025Megan J. R. Yeo, Olivia Zhang, Xiaowen Xie, Eunju Nam, N. Connor Payne, Pallavi M. Gosavi, Hui Si Kwok, Irtiza Iram, Ceejay Lee, Jiaming Li, et al.Thermo Fisher Scientific (B85502 and A35243, respectively), with Grace insect medium (Thermo Fisher Scientific, 11595030) supplemented with 10% FBS (Cytiva) and 1% penicillin–streptomycin (Gibco), cultured at 26 °C. All of

(5) Protocol to culture Leishmania protozoans for lipophosphoglycan extraction and purificationSTAR ProtocolsDecember 20, 2024Lisa Ulrike Teufel, Jéssica Cristina dos Santoscalcium and magnesium,Lonza,CAT# BE17-516F Grace’s insect medium (1×) non-supplemented,Gibco,CAT # 11595030 Glutamine supplement,Life Technologies,CAT # 35050087 Penicillin/streptomycin,Life Technologies,CAT

(6) Feeding a rich diet supplemented with the translation inhibitor cycloheximide decreases lifespan and ovary size in DrosophilaBiology OpenNovember 15, 2024Hye Jin Hwang, Rachel T. Coxconcentrations of CHX. Fly ovaries were dissected in Grace's Insect Medium (Invitrogen, Waltham, MA, USA; 11595030) 24 h after CHX feeding. To measure whole ovary size, the dissected ovaries were immediately imaged

(7) Drosophila Clueless ribonucleoprotein particles display novel dynamics that rely on the availability of functional protein and polysome equilibriumbioRxivAugust 27, 2024Hye Jin Hwang, Kelsey M. Sheard, Rachel T. CoxTable. Immunostaining Ovaries were dissected with Grace’s Insect Medium (Invitrogen, Waltham, MA, USA, cat#11595030) and fixed for 20 minutes (4% paraformaldehyde and 20 mM formic acid in Grace’s Insect Medium). After

(8) Aurora-A condensation mediated by BuGZ aids its mitotic centrosome functionsiScienceMay 17, 2024Hui Zheng, Qiaoqiao Zhang, Xing Liu, Fan Shi, Fengrui Yang, Shengqi Xiang, Hao Jiang,Thermo Fisher Scientific,Cat#11605094 "Grace’s Insect Medium, unsupplemented",Thermo Fisher Scientific,Cat# 11595030 FBS,Excell,Cat#FSP500 RNAiMAX,Thermo Fisher Scientific,Cat# 13778150 Lipo 3000,Thermo Fisher Scientific,Cat#

(9) Adult neurogenesis does not explain the extensive post-eclosion growth of Heliconius mushroom bodiesRoyal Society Open ScienceOctober 25, 2023Amaia Alcalde Anton, Fletcher J. Young, Lina Melo-Flórez, Antoine Couto, Stephen Cross, W. Owen McMillan, Stephen H. Montgomerybutterflies. They were then incubated in 20 µM EdU solution diluted in Grace's Medium (Thermo-Fisher, #11595030) for 3 h. After the incubation brains were dissected out and fixed in 4% paraformaldehyde (PFA) in 0.1

(10) A modified method to analyse cell proliferation using EdU labelling in large insect brainsPLOS ONEOctober 5, 2023Amaia Alcalde Anton, Max S. Farnworth, Laura Hebberecht, C. Jill Harrison, Stephen H. Montgomerymedium, we tested the benefits of replacing the dilution buffer with Grace’s Medium (ThermoFisher, #11595030) which is used to maintain insect cell cultures [49]. To refine the EdU protocol we focused on larvae

(11) Glycolytically impaired Drosophila glial cells fuel neural metabolism via β-oxidationNature CommunicationsMay 24, 2023Ellen McMullen, Helen Hertenstein, Katrin Strassburger, Leon Deharde, Marko Brankatschk, Stefanie Schirmeierstainings Brains from adult mated females were dissected in Graces medium (Thermo Fisher Scientific 11595030) on ice, fixed with 4% PFA in Graces medium at RT for 30 min and probed with anti-Brp (DHB clone NC82)

(12) Chemical and structural approaches to investigate PTEN function and regulationMethods in EnzymologyMarch 22, 2023Thibault Viennet, Santiago Rodriguez Ospina, Yunqi Lu, Anna Cui, Haribabu Arthanari, Daniel R. Dempsey"Grace’s Insect Medium, supplemented",ThermoFisher Scientific,11605094 "Grace’s Insect Medium, unsupplemented",ThermoFisher Scientific,11595030 Express Five SFM,ThermoFisher Scientific,10486025 Fetal Bovine Serum (FBS),Millipore Sigma,F0926 L-Glutamine

(13) Southern rice black-streaked dwarf virus induces incomplete autophagy for persistence in gut epithelial cells of its vector insectPLoS pathogensJanuary 1, 2023Zhang L, Liu W, Wu N, Wang H, Zhang Z, Liu Y, Wang X.Cellfection II was added to each well and incubated in unsupplemented Grace’s Insect Medium (Thermo Fisher, 11595030) at 27°C for 5 h. Then the transfection mixture was replaced with Sf-900 III serum-free medium to incubate

(14) A lipid transfer protein ensures nematode cuticular impermeabilityiScienceNovember 18, 2022Ferdinand Ngale Njume, Adria Razzauti, Miguel Soler, Veronika Perschin, Gholamreza Fazeli, Axelle Bourez, Cedric Delporte, Stephen M. Ghogomu, Philippe Poelvoorde, Simon Pichard, et al.lines ,, Adapted Sf9 cells,Thermofisher,Cat# B82501 Grace Medium supplemented with 10% FCS,Thermofisher,Cat# 11595030 Adapted High Five cells,Thermofisher,Cat# B85502 Express Five medium,Thermofisher,Cat#10486025 ,, Experimental models

(15) Dot-Blotting: A Quick Method for Expression Analysis of Recombinant ProteinsCurr ProtocSeptember 1, 2022Mishra V.Thermo Fisher Scientific, cat. no: 12658019 Grace’s Insect Medium, Unsupplemented, Thermo Fisher Scientific, cat.no: 11595030 FuGENE® HD Transfection Reagent, Promega, cat.no. E2311 Gibco™ Antibiotic-Antimycotic, cat. no:15240112

(16) Extracellular domain of PepT1 interacts with TM1 to facilitate substrate transportStructureJuly 1, 2022Jiemin Shen, Miaohui Hu, Xiao Fan, Zhenning Ren, Corinne Portioli, Xiuwen Yan, Mingqiang Rong, Ming ZhouHealthcare,Cat#SH30278.02 Grace’s Insect Medium Supplemented,Gibco,Cat#11605–094 Grace’s Insect Medium Unsupplemented,Gibco,Cat#11595030 Fetal Bovine Serum,Gibco,Cat#A4736301 n-Dodecyl-β-D-Maltopyranoside,Anatrace,Cat#D310 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1’-rac)-choline

(17) Genetic Code Expansion System for Tight Control of Gene Expression in Bombyx mori Cell LinesInsectsDecember 1, 2021Wei Lu, Ruolin Wang, Pan Wang, Sanyuan Ma, Qingyou XiaLaboratory of Silkworm Genome Biology. The cells were grown on Grace’s insect medium (Gibco, NY, USA, 11595030) and TC-100 insect medium (Sigma, St. Louis, MO, USA, T3160) supplemented with 10% (v/v) fetal bovine

(18) piRNA-independent transposon silencing by the Drosophila THO complexDevelopmental cellSeptember 27, 2021Zhang G, Yu T, Parhad SS, Ho S, Weng Z, Theurkauf WE.dUTP Solution (100 mM),ThermoFisher Scientific,Cat# R0133 "Grace's Insect Medium, unsupplemented",ThermoFisher Scientific,Cat# 11595030 Hybridase,Lucigen,Cat# H39500 dNTP mix,NEB,Cat# N0447L DNA polymerase I,NEB,Cat# M0209S T4 DNA polymerase,NEB,Cat#

(19) Fibronectin-based nanomechanical biosensors to map 3D surface strains in live cells and tissueNature CommunicationsDecember 1, 2020Daniel J. Shiwarski, Joshua W. Tashman, Alkiviadis Tsamis, Jaci M. Bliley, Malachi A. Blundon, Edgar Aranda-Michel, Quentin Jallerat, John M. Szymanski, Brooke M. McCartney, Adam W. Feinbergday, ovaries were extracted from the female flies in 22 °C unsupplemented Grace’s media (Thermo, Cat#: 11595030) using standard Dumont forceps. For each set of ovaries, the outer fibrous sheath was removed and individual

(20) Expression and Purification of Functionally Active Serotonin 5-HT2A Receptor in Insect Cells Using Low-titer Viral StockBIO-PROTOCOLAugust 5, 2020Sukanya Mozumder, Gopa Mahesh, Krishnamoorthi Srinivasan, Jayati Sengupta, Sujoy Mukherjeecatalog number: 96-001-01) 11. Grace’s Insect cell culture medium (Life Technologies, catalog number: 11595030) 12. Production Boost Additive (Expression Systems, catalog number: 95-006-100) 13. Cellfectin II (Life

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Grace's Insect Medium, unsupplemented from Thermo Fisher Scientific

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Grace's Insect Medium, unsupplemented from Thermo Fisher Scientific

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