Trypsin Neutralizing Solution from ATCC

Product Specs
ItemTrypsin Neutralizing Solution
CompanyATCC
Price $26.00Supplier Page View Company Product Page
Catalog NumberPCS-999-004
Format5% FBS in phosphate buffered saline with calcium and magnesium; pH: 7.5 ± 0.3; Osmolality: 290 ± 20 20 mOsm.
ApplicationsPlease refer to the product sheet supplied with each primary cell for details as to use, or contact us
TypeMedia Sera Reagents
Volume100 mL

Specifications/Features

Sterility:
Negative for bacteria, fungi and yeast.

Storage:
-20°C or colder

Functional Testing:
Each lot is assessed for cell passaging activity.

Add to Compare List

ATCC

10801 University Boulevard

Manassas, Virginia 20110-2209

United States

Phone: (703) 365-2700
Fax: (703) 365-2701

Company Profile

Website: http://www.atcc.org

(1) High-Content Imaging and Machine Learning Classify Phenotypical Change in Coronary Artery Endothelial Cells Caused by BPSInt J Mol SciApril 3, 2026Lavinia Eugenia Ferariu, Gheorghe Movileanu, Giulia Gaggi, Barbara Ghinassi, Angela di Baldassarre, Andrea Di CredicoEDTA; ATCC® PCS-999-003™, USA), followed by neutralisation with Trypsin Neutralising Solution (ATCC® PCS-999-004™, USA). Cells were fed every 48 h with fresh complete medium. All experiments were conducted with cells

(2) Houttuynia cordata Exhibits Anti-Inflammatory Activity Against Interleukin-1β-Induced Inflammation in Human Gingival Epithelial Cells: An In Vitro StudyDentistry journalAugust 7, 2025Kunimatsu R, Ikeoka S, Koizumi Y, Odo A, Tanabe I, Kawashima Y, Kiso A, Hashii Y, Tsuka Y, Tanimoto K.Trypsin-EDTA for Primary Cells (PCS-999-003, ATCC), neutralized with Trypsin Neutralizing Solution (PCS-999-004, ATCC), and subcultured in experimental plates. 2.3. Cell Proliferation Assay Precultured PGKs were

(3) Hyaluronic Acid Microparticles Promote Uniform Differentiation and Survival of Stem Cells in Spheroid CulturesACS OmegaJuly 28, 2025Hye-Eun Shim, Yong-Jin Kim, Kyoung Hwan Park, Jean Choi, Yu Bin Lee, Kang Moo Huh, Sun-Woong Kangconfluence using Trypsin-EDTA for Primary Cells (ATCC, PCS-999-003) and Trypsin Neutralizing Solution (ATCC, PCS-999-004). To validate the identity of the AdMSCs, flow cytometry (FCM) analysis was performed to assess the

(4) The proliferation rates of HT-1080 human fibrosarcoma cells can be accelerated or inhibited by weak static and extremely low frequency magnetic fieldsFront Public HealthJune 12, 2025Marek Bajtoš, Nhat Dang, Isabel Lopez de Mingo, Jason Keller, Hakki Gurhan, Ladislav Janoušek, Frank Barnes(D-PBS; ATCC 30–2,200) at a volume of 5 mL per flask. After discarding the D-PBS, 1.5 mL of trypsin (ATCC PCS-999-004) was added per flask and the flasks/cells were returned to the incubator for 5 min. Cell detachment

(5) Integrated analysis of transcriptional and metabolic responses to mitochondrial stressCell Reports MethodsApril 21, 2025Liam P. Kelley, Song-Hua Hu, Sarah A. Boswell, Peter K. Sorger, Alison E. Ringel, Marcia C. HaigisFibroblast Growth Kit-Low serum,ATCC,PCS-201-041 Trypsin-EDTA for Primary Cells,ATCC,PCS-999-003 Trypsin Neutralizing Solution,ATCC,PCS-999-004 "Penicillin-Streptomycin (10,000 U/mL)",Gibco,15140122 "RPMI 1640 Medium w/o Amino Acids, Sodium Phosphate",US

(6) Upregulation of HPV16E1 and E7 expression and FOXO3a mRNA downregulation in high-grade cervical neoplasiaPeerJDecember 6, 2024Thanayod Sasivimolrattana, Aileen Gunawan, Warattaya Wattanathavorn, Chavis Pholpong, Arkom Chaiwongkot, Pattarasinee Bhattarakosol, Parvapan Bhattarakosolwith Trypsin-EDTA for Primary Cells (ATCC PCS-999-003) and the Trypsin Neutralizing Solution (ATCC PCS-999-004). Cervical Epithelial Cell Basal Medium (ATCC PCS-480-032) was used as a complete growth medium. The

(7) Almond Hull Extract Valorization: From Waste to Food Recovery to Counteract Staphylococcus aureus and Escherichia coli in Formation and Mature BiofilmFoodsNovember 28, 2024Sara D’Arcangelo, Debora Santonocito, Luciano Messina, Valentina Greco, Alessandro Giuffrida, Carmelo Puglia, Mara Di Giulio, Rosanna Inturri, Susanna Vaccarofollowed by harvesting with Trypsin-EDTA (ATCC® PCS-999-003) and Trypsin Neutralizing Solution (ATCC® PCS-999-004). The fresh medium was added to the cells to reach a final concentration of approximately 1.0 × 104

(8) Ferroptosis of select skin epithelial cells initiates and maintains chronic systemic immune-mediated psoriatic diseaseJournal of Clinical InvestigationNovember 21, 2024Kavita Vats, Hua Tian, Kunal Singh, Yulia Y. Tyurina, Louis J. Sparvero, Vladimir A. Tyurin, Oleg Kruglov, Alexander Chang, Jiefei Wang, Felicia Green, et al.confluence at or below passage 5. Trypsin-EDTA (ATCC PCS-999-003) and Trypsin Neutralizing Solution (ATCC PCS-999-004) were used to detach the primary cells. Human tissue. Tissue samples from pulmonary airways of patients

(9) Three-Dimensional Cultured Human Dermal Papilla Cells in HGC-Coated Environments Enhance Hair Regeneration and Artificial Skin IntegrationBiomaterials ResearchApril 12, 2024Thuy Trang Truong, Heejin Park, Kyoung Hwan Park, Jin Jung Song, Byoung-Seok Lee, Kang Moo Huh, Sun-Woong Kangupon reaching 80% confluency, utilizing trypsin EDTA/Neutralizing Solution (ATCC PCS-999-003/ ATCC PCS-999-004, Manassas, VA, USA). All cells were cultured at 37 °C in a humidified atmosphere with 5% CO₂. The medium

(10) Development of a Probability-Based In Vitro Eye Irritation Screening PlatformBioengineering (Basel)March 26, 2024Seep Arora, Anna Goralczyk, Sujana Andra, Soon Yew John Lim, Yi-Chin Tohcells were subsequently neutralized by Trypsin neutralizing solution (TNS, ATCC, Manassas, VA, USA, PCS-999-004) and re-suspended in the complete medium after centrifugation for 5 min at 1000 rpm and later counted

(11) Exploiting the Features of Short Peptides to Recognize Specific Cell Surface MarkersInternational Journal of Molecular SciencesOctober 26, 2023Michela Buonocore, Manuela Grimaldi, Angelo Santoro, Verdiana Covelli, Carmen Marino, Enza Napolitano, Sara Novi, Mario Felice Tecce, Elena Ciaglia, Francesco Montella, et al.80–90%, cells were detached using 0.05% solution of trypsin-EDTA and trypsin neutralizing solution (ATCC® PCS-999-004) was used to stop trypsin digestion of cells. The split cells were centrifuged for 5 min at 1500 rpm

(12) Weak Radiofrequency Field Effects on Chemical Parameters That Characterize Oxidative Stress in Human Fibrosarcoma and Fibroblast CellsBiomoleculesJuly 13, 2023Hakki Gurhan, Marek Bajtoš, Frank BarnesPhosphate-Buffered Saline (D-PBS) (ATCC 30-2200) (5 mL per flask). D-PBS was removed, and 1.5 mL of trypsin (ATCC PCS-999-004) was added per flask. The cells were placed in the incubator for five minutes. The culture flask was

(13) Effects of the Co-Overexpression of the BCL and BDNF Genes on the Gamma-Aminobutyric Acid-Ergic Differentiation of Wharton's-Jelly-Derived Mesenchymal Stem CellsBiomedicinesJune 18, 2023Borkowska P, Morys J, Zielinska A, Kowalski J.solution (5% FBS (fetal bovine serum) in phosphate-buffered saline without calcium and magnesium) (#PCS-999-004; ATCC®, University Blvd, Manassas, VA, USA) was used. The volume of trypsin and trypsin inactivator

(14) Conserved transcription factors promote cell fate stability and restrict reprogramming potential in differentiated cellsNature communicationsMarch 27, 2023Missinato MA, Murphy S, Lynott M, Yu MS, Kervadec A, Chang YL, Kannan S, Loreti M, Lee C, Amatya P, et al.trypsin-EDTA solution for primary cells (ATCC, PCS-999-003) for 3 min at 37 °C, washed with trypsin-neutralizing solution (ATCC, PCS-999-004), resuspended in VCBM/kit-BBE medium, added to 384-well plates at 3 × 103 cells/well, and transfected

(15) HIF-1α is a negative regulator of interferon regulatory factors: Implications for interferon production by hypoxic monocytesProceedings of the National Academy of SciencesJune 29, 2021Travis Peng, Shin-Yi Du, Myoungsun Son, Betty DiamondPCS999003) containing 0.05% trypsin and 0.02% EDTA followed by Trypsin Neutralizing Solution (ATCC, PCS999004) according to the manufacturer’s recommendation. All reagents for cell cultures were purchased from

(16) Intracellular Ca2+-Mediated AE2 Is Involved in the Vectorial Movement of HaCaT KeratinocyteInternational Journal of Molecular SciencesNovember 10, 2020Soyoung Hwang, Dong Min Shin, Jeong Hee Hong(PCS-999-003) for 6 min. When cells appear to have detached, add the trypsin neutralizing solution (ATCC PCS-999-004) to flask. The dispersed primary keratinocytes were transferred to new culture dishes for qRT-PCR. 4.3.

(17) Cystathione β-synthase regulates HIF-1α stability through persulfidation of PHD2Science advancesJuly 1, 2020Dey A, Prabhudesai S, Zhang Y, Rao G, Thirugnanam K, Hossen MN, Dwivedi SKD, Ramchandran R, Mukherjee P, Bhattacharya R.from ATCC). Trypsin-EDTA for primary cells (PCS-999-003 from ATCC) and trypsin neutralizing solution (PCS-999-004 from ATCC) were used for HAOEC subculture. All experiments were performed only between passages 3 and

(18) Cytotoxicity of Formulated Graphene and Its Natural Rubber Nanocomposite Thin Film in Human Vaginal Epithelial Cells: An Influence of Noncovalent InteractionACS Biomaterials Science & EngineeringApril 13, 2020Thenmozhi Sukumar, Jeslin Varghese, Kiran S, Suja Bhargavan, Parvathy Jayasree, Vemparthan Suvekbala, Kumaran Alaganandam, Lakshminarayanan Ragupathyvaginal epithelial (HVE) cells [obtained from ATCC (American Type Culture Collection), #PCS-999-003, #PCS-999-004] was conducted as described above with slight modifications, such as 4000 cells/well in 96-well plates

(19) Deep-Ultraviolet Raman Spectroscopy for Cancer Diagnostics: A Feasibility Study with Cell Lines and TissuesCancer Studies and Molecular Medicine – Open JournalOctober 29, 2019Nicole M. Ralbovsky et al.the cells from the surface of the flask. An equal volume of the Trypsin Neutralizing Solution (ATCC PCS-999-004) was added to the flask and the cells were collected in a 15 mL tube. The tube containing the trypsin-EDTA-dissociated

(20) The alpha-1 subunit of the Na+,K+-ATPase (ATP1A1) is required for macropinocytic entry of respiratory syncytial virus (RSV) in human respiratory epithelial cellsPLoS pathogensAugust 1, 2019Lingemann M, McCarty T, Liu X, Buchholz UJ, Surman S, Martin SE, Collins PL, Munir S.trypsin-neutralizing solution formulated specifically for primary cells (ATCC PCS-999-003 and ATCC PCS-999-004. The primary cells were passaged a maximum of two times. HAE-ALI cultures (EpiAirway, AIR-100) were

Be the First to Write a Review!

Trypsin Neutralizing Solution from ATCC

Description

Specifications/Features

ATCC

Citations (26)

Reviews

Trypsin Neutralizing Solution from ATCC

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map