Flexi Rabbit Reticulocyte Lysate System from Promega

Product Specs
ItemFlexi Rabbit Reticulocyte Lysate System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberL4540
Quantity30 reactions
SystemRabbit Reticulocyte
SpeciesRabbit

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) A Novel Eukaryotic Ribosome Factor Enables Translation Restart Following Cellular DormancybioRxivMarch 27, 2026Maciej Gluc, Higor Rosa, Maria Bozko, Lesley A Turner, Cassidy R Prince, Yelena Peskova, Heather A Feaga, Kathleen L Gould, Simone Mattei, Ahmad JomaaN-terminal 3x Flag-tagged reporter was translated in rabbit reticulocyte lysate (RRL; Promega, cat. no. L4540). The lysate was diluted to 66.7% (v/v) with a translation mix containing purified RNA (final concentration

(2) Mechanism of ribosome stalling by the AMD1 C-terminal tail arrest peptideSci AdvMarch 27, 2026Emir Maldosevic, Fabio S. Boiocchi, Michal I. Swirski, Kyle A. Meiklejohn, Martina M. Yordanova, Pavel V. Baranov, Ahmad Jomaaand XBP1 control were in vitro translated in FLEXI rabbit reticulocyte lysate (Promega, catalog no. L4540) diluted to 66.7% (v/v) in buffer [mRNA (0.5 μg/μl), 81 mM KCl, 0.67 mM Mg(OAc)2, 24 μM amino acid mix,

(3) A ribosome-bound pseudoknot in the HCV coding region stimulates viral growth by tuning viral translationCell ReportsJanuary 27, 2026Han Wan, Harim Jang, Ling Xu, Kyrillos S. Abdallah, Wendy V. Gilbert, Anna Marie Pylepull down” section, with modifications. Specifically, 200 μL of Rabbit reticulocyte lysate (Promega L4540) was used, spiked with 5 mM MgCl2 and 100 mM KCl. The elution sample was diluted to 0.1 mg/mL and 4

(4) RNA G-quadruplexes promote codon repeat-associated ribosomal frameshifting in human genesNucleic Acids ResearchJanuary 16, 2026Xiuwen Li, Zhanbiao Li, Yingshui Zhou, Shimin Gong, Zhenjing Chen, Jingyang Li, Miaomiao Guo, Xiaoqian Gu, Fei Li, Jiayu Wei, et al.lysis buffer (Promega, E1941). For in vitro translation using rabbit reticulocyte lysate (Promega, L4540), a modified reaction system was employed. Each 10 μl reaction contained: 7 μl of lysate, 0.2 μl of

(5) NAC couples protein synthesis with nascent polypeptide myristoylation on the ribosomeThe EMBO journalNovember 1, 2025Zdancewicz S, Maldosevic E, Malezyna K, Jomaa A.crude RNCs were generated by reacting RRL from a Flexi Rabbit Reticulocyte Lysate System (Promega, L4540) with mRNA synthesized by in vitro transcription as described above. For the in vitro translation reaction

(6) A flexible, high-throughput system for studying live mRNA translation with HiBiT technologyNucleic Acids ResJune 16, 2025Camilla Ascanelli, Elsa Lawrence, Christopher A P Batho, Catherine H Wilsonperformed by adapting the protocol of Promega’s Flexi Rabbit Reticulocyte Lysate Kit (nuclease treated, L4540). Given the different sizes of the mRNA products, the comparison between constructs must be made with

(7) The clinical antiprotozoal drug halofuginone promotes weight loss by elevating GDF15 and FGF21Science advancesMarch 28, 2025Xu S, Liu Z, Tian T, Zhao W, Wang Z, Liu M, Xu M, Zhang F, Zhang Z, Chen M, et al.experiment with rabbit reticulocytes The Flexi Rabbit Reticulocyte Lysate System kit from Promega (L4540, WI, USA) was used for the experiments per the user guide. In summary, all required components for in vitro translation

(8) Coordination of cytochrome bc1 complex assembly at MICOSEMBO ReportsDecember 2, 2024Ralf M Zerbes, Lilia Colina-Tenorio, Maria Bohnert, Karina von der Malsburg, Christian D Peikert, Carola S Mehnert, Inge Perschil, Rhena F U Klar, Rinse de Boer, Anita Kram, et al.Biosciences,60105 [35S]Methionine,PerkinElmer,NEG009T005MC KOD Hot Start Master Mix,Novagen,71842-3 Flexi Rabbit Reticulocyte Lysate,Promega,L4540 TNT Quick Coupled Reaction Mix,Promega,L2080 CNBr-activated Sepharose 4B,GE Healthcare,17-0430-01 Mitochondrial

(9) The microprotein HDSP promotes gastric cancer progression through activating the MECOM-SPINK1-EGFR signaling axisNature CommunicationsSeptember 27, 2024Yuli Chen, Qiuhui Li, Xiang Yu, Lu Lu, Zihan Zhou, Mingjie Li, Rui Xia, Xiongkang Gan, Yanming Hu, Guoqing Guo, et al.to in vitro transcription/translation using a rabbit reticulocyte-coupled system (Promega, WI, USA, L4540) supplemented with [35S] methionine for a duration of 1 h. Following the incubation period, the translated

(10) RNA-modifying enzyme Alkbh8 is involved in mouse embryonic developmentiScienceSeptember 20, 2024Manami Nakai, Hiroaki Hase, Yutong Zhao, Katsuya Okawa, Kohei Honda, Kaori Ikuma, Kaori Kitae, Kazutake Tsujikawa,Thermo Fisher SCIENTIFIC,Cat#11755050 THUNDERBIRD® SYBR™ qPCR Mix,TOYOBO,QPS-201 Flexi® Rabbit Reticulocyte Lysate System,Promega,Cat#L4540 Luciferase Assay Reagent,Promega,Cat#E1483 BCA Protein Assay,Thermo Fisher SCIENTIFIC,Cat#23227 "Clariom™

(11) Deep mutational scanning of hepatitis B virus reveals a mechanism for cis-preferential reverse transcriptionCellMay 23, 2024Yingpu Yu, Maximilian A. Kass, Mengyin Zhang, Noor Youssef, Catherine A. Freije, Kelly P. Brock, Lauren C. Aguado, Leon L. Seifert, Sanjana Venkittu, Xupeng Hong, et al.mutant RNAs (0.1 μg) were translated using the Lexi® Rabbit Reticulocyte Lysate System (Promega, cat. # L4540) in a 20 μl reaction volume supplemented with 25 mM KCl, 1.25 mM MgOAc, and 0.2 mCi/ml [35S]-methionine

(12) A highly optimized human in vitro translation systemCell Rep MethodsApril 22, 2024Bothe A, Ban N.IVT kit,Thermo Fisher Scientific,Cat# 88881 Flexi® Rabbit Reticulocyte Lysate System,Promega,Cat# L4540 EX-CELL® HeLa Serum-Free Medium,SAFC,Cat# 14591C ,, Critical commercial assays,Critical commercial

(13) Identification of MIMAS, a multifunctional mega-assembly integrating metabolic and respiratory biogenesis factors of mitochondriaCell ReportsMarch 26, 2024Patrick Horten, Kuo Song, Joshua Garlich, Robert Hardt, Lilia Colina-Tenorio, Susanne E. Horvath, Uwe Schulte, Bernd Fakler, Martin van der Laan, Thomas Becker, et al.commercial assays ,, KOD Hot Start Master Mix,Novagen,71842–3 Flexi Rabbit Reticulocyte Lysate,Promega,L4540 TNT Quick Coupled Reaction Mix,Promega,L2080 ,, Experimental models: Organisms/strains,Experimental models

(14) An acetyltransferase effector conserved across Legionella species targets the eukaryotic eIF3 complex to modulate protein translationmBioMarch 13, 2024Lukas Syriste, Deepak T. Patel, Peter J. Stogios, Tatiana Skarina, Dhruvin Patel, Alexei Savchenkoprotein translation assays were performed using a Promega Flexi rabbit reticulocyte lysate system (L4540). The experiment was performed following the protocol of the manufacturer. In brief summary, tubes were

(15) N1-methylpseudouridylation of mRNA causes +1 ribosomal frameshiftingNatureJanuary 4, 2024Thomas E. Mulroney, Tuija Pöyry, Juan Carlos Yam-Puc, Maria Rust, Robert F. Harvey, Lajos Kalmar, Emily Horner, Lucy Booth, Alexander P. Ferreira, Mark Stoneley, et al.were translated using the Flexi Rabbit Reticulocyte Lysate System using nuclease-treated RRL (Promega L4540). For co-translational labelling, 0.33 µl translation-grade [35S]methionine (Hartman Analytic KSM-01)

(16) Single-molecule imaging reveals distinct elongation and frameshifting dynamics between frames of expanded RNA repeats in C9ORF72-ALS/FTDNature communicationsSeptember 11, 2023Latallo MJ, Wang S, Dong D, Nelson B, Livingston NM, Wu R, Zhao N, Stasevich TJ, Bassik MC, Sun S, et al.Then, 500 ng mRNAs were in vitro translated with Flexi Rabbit Reticulocyte Lysate System (Promega, L4540), following the manufacturer’s protocol. The reactions were incubated at 30 °C for 30 min before termination

(17) G-quadruplex in hepatitis B virus pregenomic RNA promotes its translationJournal of Biological ChemistrySeptember 1, 2023Jingjing Wang, Haiyan Huang, Kaitao Zhao, Yan Teng, Li Zhao, Zaichao Xu, Yingcheng Zheng, Lu Zhang, Conghui Li, Yurong Duan, et al.in vitro translation were performed with Flexi Rabbit Reticulocyte Lysate System (Promega Corporation, L4540) according to the manufacturer’s protocol. Lysates were then subjected to Western blot. Statistical

(18) In vitro Analysis of Stalled Ribosomes Using Puromycin IncorporationBio ProtocAugust 20, 2023MaKenzie R. Scarpitti, Michael G. Kearse(Thermo Scientific, catalog number: 15585011)Flexi Rabbit Reticulocyte Lysate System (Promega, catalog number: L4540)37% (w/v) formaldehyde (Fisher Chemical, catalog number: F79-500)Glycerol, biotechnology grade (Amresco,

(19) FUS regulates RAN translation through modulating the G-quadruplex structure of GGGGCC repeat RNA in C9orf72-linked ALS/FTDeLifeJuly 18, 2023Yuzo Fujino, Morio Ueyama, Taro Ishiguro, Daisaku Ozawa, Hayato Ito, Toshihiko Sugiki, Asako Murata, Akira Ishiguro, Tania Gendron, Kohji Mori, et al.,Thermo Fisher Scientific,Cat# AM1344, Commercial assay or kit,Flexi Rabbit Reticulocyte Lysate System,Promega,Cat# L4540, "Chemical compound, drug",RU486 (mifepristone),Wako,M3321; CAS: 84371-65-3, "Chemical compound, drug",Formula

(20) Translation re-initiation after uORFs does not fully protect mRNAs from nonsense-mediated decayRNAJune 1, 2023Paul J. Russell, Jacob A. Slivka, Elaina P. Boyle, Arthur H.M. Burghes, Michael G. Kearsein the linear range using 3 nM mRNA in the Flexi Rabbit Reticulocyte Lysate (RRL) System (Promega # L4540) with final concentrations of reagents at 30% RRL, 10 µM amino acid mix minus leucine, 10 µM amino acid

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