Riboprobe System-T7 from Promega

Product Specs
ItemRiboprobe System-T7
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberP1440
Quantity1 system

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Regular Aerobic Exercise Can Effectively Ameliorate the Skeletal Muscle and Mitochondrial Function Impairments Caused by bves Deficiency in ZebrafishInt J Mol SciJune 20, 2026Wanwan Cai, Wanbang Zhou, Xiushan Wu, Junrong Lei, Haochen Wang, Qiong Wu, Song Zhou, Kang Sun, Xiuyan Li, Zhilong Zhang, et al.into digoxigenin-labeled antisense RNA probes using an RNA transcription kit (Promega, Wi, USA, Cat#:P1440) along with Anti-Digoxigenin-AP Fab fragments (Roche, Basel, Switzerland, Cat#:11093274910). The primer

(2) Ribonuclease DIS3 delays aging and senescence by generating tRNA halvesNature CommunicationsMay 21, 2026Seokjun G. Ha, Hanseul Lee, Jisoo Park, Donghun Lee, Gee-Yoon Lee, Seokjin Ham, Sujeong Kwon, Sangsoon Park, Sieun S. Kim, Yoonji Jung, et al.luciferase, firefly luciferase mRNA was transcribed using Riboprobe in vitro transcriptions systems (P1440, Promega, Madison, WI, USA) following the manufacturer’s protocol with minor modifications. Specifically,

(3) Heterogeneous nuclear ribonucleoprotein A1 (hnRNPA1) maintains muscle progenitor identity by stabilizing the Ppp1r1b-lncRNA-PRC2 complexNucleic Acids ResMay 19, 2026Xuedong Kang, Yan Zhao, Stanley F Nelson, April Pyle, Aldons J Lusis, Marlin Toumaassay was performed with C2C12 cell lysates. Riboprobe® System T7 and Riboprobe® System SP6 (Promega, P1440, P1420), and Biotin RNA labeling mix (Roche, 11 685 597 910) were used for the preparation of biotin-labeled

(4) BMPR2 affects valve development via ECM–receptor interaction in zebrafishFrontiers in Cell and Developmental BiologyMarch 23, 2026Yan Shi, Yanli Huang, Yu Xia, Yongqing Li, Yuequn Wang, Wuzhou Yuan, Fang Li, Zhigang Jiang, Yu Chen, Ping Zhu, et al.and subjected to in vitro transcription experiments (Riboprobe® System-T7 Translation Kit (Promega, P1440) to produce sgRNA. The sgRNA (20 ng/μL) was mixed with Cas9 protein (TrueCut Cas9 v2, Thermo Fisher Scientific

(5) Mechanical control of histone serotonylation initiates neural crest migration in vivobioRxivOctober 25, 2025Joana E. Saraiva, Artemis G. Korovesi, Xiaoran Wei, Jaime A. Espina, Miguel Ribeiro, Ian Maze, Elias H. BarrigaPCRs was 62°C. Transcription was performed using the Promega Riboprobe® in vitro transcription system (P1440 for the T7 RNA polymerase kit) as aforementioned. Probes were used at a concentration of 3 μg/ml in

(6) Construction of a Zebrafish Model of Cardiac Hypertrophy Caused by ATIC Gene Deletion and Preliminary Exploration of Aerobic Exercise ImprovementInternational Journal of Molecular SciencesOctober 22, 2025Tianle Yang, Zhilong Zhang, Shuaiwang Huang, Mengchao Cui, Siyuan Liu, Meng Ding, Wenzhi Gu, Boyu Yang, Lan ZhengThe sgRNA was synthesized by in vitro transcription using the Riboprobe® System-T7 Transcription Kit (P1440, Promega, Madison, WI, USA). The sgRNA (final concentration: 20 ng/μL each) was co-injected into single-cell-stage

(7) The Interaction Between the asb5a and asb5b Subtypes Jointly Regulates the L-R Asymmetrical Development of the Heart in ZebrafishInt J Mol SciMarch 19, 2025Wanbang Zhou, Wanwan Cai, Yongqing Li, Luoqing Gao, Xin Liu, Siyuan Liu, Junrong Lei, Jisheng Zhang, Yuequn Wang, Zhigang Jiang, et al.antisense RNA probes via in vitro transcription, utilizing the Riboprobe® System-T7 transcription kit (P1440, Promega, Madison, WI, USA) and the ROACH DIG RNA Labeling Mix (REF 11277073910, Roche, Basel, Switzerland).

(8) Mice deficient in TWIK-1 are more susceptible to kainic acid-induced seizuresiScienceJanuary 17, 2025Ajung Kim, Yeonju Bae, Changdev G. Gadhe, Hyun-Gug Jung, Esther Yang, Hyun Kim, Jaekwang Lee, Chanseob Shim, Young Hoon Sung, Junyeol Noh, et al.generated using 35S-uridine triphosphate (PerkinElmer #NEG039H250UC) and the Riboprobe system (Promega, P1440). The probes were labeled with α-35S-dATP deoxynucleotidyl transferase (GIBCO BRL). The sections were

(9) Exo1 cooperates with Tel1/ATM in promoting recombination events at DNA replication forksiScienceAugust 16, 2024Michela Galli, Chiara Frigerio, Chiara Vittoria Colombo, Erika Casari, Maria Pia Longhese, Michela Clericicommercial assays,Critical commercial assays,Critical commercial assays ,, Riboprobe System-T7,Promega,Cat#P1440 Invitrogen™ DECAprime™ II DNA Labeling Kit,Invitrogen,Cat#AM1455 QIAGEN QIAquick PCR Purification

(10) Proteasome-mediated degradation of long-range nucleases negatively regulates resection of DNA double-strand breaksiScienceJuly 19, 2024Marco Gnugnoli, Carlo Rinaldi, Erika Casari, Paolo Pizzul, Diego Bonetti, Maria Pia Longhesecommercial assays,Critical commercial assays,Critical commercial assays ,, Riboprobe System-T7,Promega,Cat#P1440 DECAprime™ II DNA Labeling Kit,Invitrogen,Cat# AM1456 QIAGEN QIAquick PCR Purification Kit,QIAGEN,Cat#28106 QIAquick

(11) hnRNPM protects against the dsRNA-mediated interferon response by repressing LINE-associated cryptic splicingMolecular CellJune 6, 2024Rong Zheng, Mikayla Dunlap, Georg O.M. Bobkov, Carlos Gonzalez-Figueroa, Khushali J. Patel, Jingyi Lyu, Samuel E. Harvey, Tracey W. Chan, Giovanni Quinones-Valdez, Mudra Choudhury, et al.In-vitro RNA transcripts were produced using Riboprobe® Systems T3 (Promega, P1430; MED15) or T7 (Promega, P1440; TRAPPC10) kits according to the manufacturers manual. After DNAse digest, transcripts were extracted

(12) Evaluation of different genomic regions of rotavirus B and rotavirus C for development of real-time RT‒PCR assaysVirology JournalApril 24, 2024Madhuri S. Joshi, Manohar S. Shinde, Mallika LavaniaHilden, Germany). In vitro, transcribed (IVT) RNA was synthesized using a T7 Riboprobe® System (Cat No: P1440; Promega, USA) according to the manufacturer's instructions. The concentration of synthetic fragments

(13) Sulfated disaccharide protects membrane and DNA damages from arginine-rich dipeptide repeats in ALSScience advancesFebruary 23, 2024Chang YJ, Lin KT, Shih O, Yang CH, Chuang CY, Fang MH, Lai WB, Lee YC, Kuo HC, Hung SC, et al.respectively. Transcription assay Transcription assay in vitro was conducted using Riboprobe Systems (catalog no. P1440, Promega, WI, USA) following the protocol provided by the manufacturer. The active reagent was prepared

(14) asb5a/asb5b Double Knockout Affects Zebrafish Cardiac Contractile FunctionInternational Journal of Molecular SciencesNovember 15, 2023Wanwan Cai, Yuequn Wang, Ying Luo, Luoqing Gao, Jian Zhang, Zhigang Jiang, Xiongwei Fan, Fang Li, Yulian Xie, Xiushan Wu, et al.sgRNA was synthesized via in vitro transcription using the Riboprobe® System-T7 Transcription Kit (P1440, Promega, Madison, WI, USA). The sgRNAs (final concentration: 20 ng/µL each) were co-injected with Invitrogen

(15) Mitochondrial haplotype mutation alleviates respiratory defect of MELAS by restoring taurine modification in tRNA with 3243A > G mutationNucleic Acids ResearchAugust 11, 2023Saori Ueda, Mikako Yagi, Ena Tomoda, Shinya Matsumoto, Yasushi Ueyanagi, Yura Do, Daiki Setoyama, Yuichi Matsushima, Asuteka Nagao, Tsutomu Suzuki, et al.run-off transcription was performaed by using a Riboprobe®in vitro Transcription Systems (Promega, P1440). The transcription reaction was performed at 37°C for 1 h in Transcription Optimized 5 × Buffer, 10

(16) Vaccination prevents severe COVID-19 outcome in patients with neutralizing type 1 interferon autoantibodiesiScienceJuly 21, 2023Wolff ASB, Hansen L, Grytaas MA, Oftedal BE, Breivik L, Zhou F, Hufthammer KO, Sjøgren T, Olofsson JS, Trieu MC, et al.protein expression; In vitro transcription and translation,"Promega, Madison, Wisconsin, US","P1420, P1430, P1440" BioPlex Pro Human IgG SARS CoV Serology assay,"BioRad, Hercules, California, US",12014634 ,, Experimental models

(17) Evaluation of droplet digital qRT-PCR (dd qRT-PCR) for quantification of SARS CoV-2 RNA in stool and urine specimens of COVID-19 patientsFrontiers in MedicineJune 26, 2023Manohar Shinde, Mallika Lavania, Jatin Rawal, Nutan Chavan, Pooja ShindeHilden, Germany). In Vitro Transcribed (IVT) RNA was synthesized using T7 Riboprobe® Systems (Cat No: P1440, Promega, United States) in accordance with the kit’s instructions. Each IVT RNA product was serially

(18) The lysine methyltransferase SMYD5 amplifies HIV-1 transcription and is post-transcriptionally upregulated by Tat and USP11Cell reportsMarch 28, 2023Boehm D, Lam V, Schnolzer M, Ott M.,StemCell Technologies,"Cat# 15062; Lot# 18A86332, 19E101792" "Riboprobe® in vitro Transcription Systems, T7",Promega,"Cat# P1440; Lot# 0000429560, 0000470577" ,, Experimental models: Cell lines,Experimental models: Cell lines,Experimental models

(19) Defective neurite elongation and branching in Nibp/Trappc9 deficient zebrafish and miceInternational journal of biological sciencesJanuary 1, 2023Hu M, Bodnar B, Zhang Y, Xie F, Li F, Li S, Zhao J, Zhao R, Gedupoori N, Mo Y, et al.740955.50 PrimerScript II Reverse Transcriptase,Takara,Cat #: 2690A Riboprobe® System,Promega,Cat #: P1420/P1440 FastStart DNA Master SYBR Green,Roche,Cat #: 12239264001 "Chemicals, peptides, and recombinant proteins","Chemicals,

(20) Efficient Detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) from Exhaled BreathThe Journal of Molecular DiagnosticsDecember 1, 2022Chaorui Duan, Luke Buerer, Jing Wang, Samuel Kaplan, Gavin Sabalewski, Gregory D. Jay, Sean F. Monaghan, Andrea E. Arena, William G. Fairbrotherby agarose gel electrophoresis. IVT was performed using the Riboprobe System-T7 kit (catalog number P1440; Promega) following the manufacturer's recommendations. The DNA template was removed by digestion with

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Riboprobe® in vitro Transcription Systems From Promega
Our lab performs in situ hybridization on a regular basis using RNA probes. We work with frozen ... Read More