Riboprobe System-SP6 from Promega

Product Specs
ItemRiboprobe System-SP6
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberP1420
Quantity1 system

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Mechanical control of histone serotonylation initiates neural crest migration in vivobioRxivOctober 25, 2025Joana E. Saraiva, Artemis G. Korovesi, Xiaoran Wei, Jaime A. Espina, Miguel Ribeiro, Ian Maze, Elias H. Barrigaantisense probe against sox862 was transcribed with a Riboprobe® in vitro Transcription System (Promega, P1420), according to the manufacturer’s instructions. DIG RNA Labeling mix (Roche 11277073910) was used. RNA cleanup

(2) Competence for neural crest induction is controlled by hydrostatic pressure through YapNature Cell BiologyApril 1, 2024Delan N. Alasaadi, Lucas Alvizi, Jonas Hartmann, Namid Stillman, Prachiti Moghe, Takashi Hiiragi, Roberto Mayorin situ hybridization was performed in accordance with previous protocol69. Riboprobe system (Promega P1420) was used to generate digoxigenin antisense probes for snai2 (ref. 10), foxd3 (ref. 17), keratin70,

(3) Vaccination prevents severe COVID-19 outcome in patients with neutralizing type 1 interferon autoantibodiesiScienceJuly 21, 2023Wolff ASB, Hansen L, Grytaas MA, Oftedal BE, Breivik L, Zhou F, Hufthammer KO, Sjøgren T, Olofsson JS, Trieu MC, et al.free protein expression; In vitro transcription and translation,"Promega, Madison, Wisconsin, US","P1420, P1430, P1440" BioPlex Pro Human IgG SARS CoV Serology assay,"BioRad, Hercules, California, US",12014634 ,, Experimental models

(4) Neural crest E-cadherin loss drives cleft lip/palate by epigenetic modulation via pro-inflammatory gene-environment interactionNature communicationsMay 24, 2023Alvizi L, Nani D, Brito LA, Kobayashi GS, Passos-Bueno MR, Mayor R.digoxigenin riboprobes was transcribed using the Riboprobe in vitro Transcription System (Promega, P1420). Embryos were fixed in MEMFA, bleached in 6% hydrogen peroxide, and then incubated with probes for

(5) Defective neurite elongation and branching in Nibp/Trappc9 deficient zebrafish and miceInternational journal of biological sciencesJanuary 1, 2023Hu M, Bodnar B, Zhang Y, Xie F, Li F, Li S, Zhao J, Zhao R, Gedupoori N, Mo Y, et al.740955.50 PrimerScript II Reverse Transcriptase,Takara,Cat #: 2690A Riboprobe® System,Promega,Cat #: P1420/P1440 FastStart DNA Master SYBR Green,Roche,Cat #: 12239264001 "Chemicals, peptides, and recombinant

(6) Cell clusters softening triggers collective cell migration in vivoNature MaterialsNovember 1, 2022Cristian L. Marchant, Abdul N. Malmi-Kakkada, Jaime A. Espina, Elias H. Barrigaa template and by following the instructions of a commercial in vitro Transcription System (Promega P1420). Templates for wild-type α-Tubulin-GFP (Addgene 56450); hyperacetylated α-Tubulin (K40Q-eGFP, Addgene

(7) Construction and characterization of two SARS‐CoV‐2 minigenome replicon systemsJournal of Medical VirologyJune 1, 2022Hu Zhang, Douglas K. Fischer, Masahiro Shuda, Patrick S. Moore, Shou‐Jiang Gao, Zandrea Ambrose, Haitao Guoamplify the DNA template from pUC57‐F1. The purified PCR product served as the template for SP6 IVT (P1420; Promega) of the radiolabeled F1 riboprobe in the following reaction mixture: 2 μl of purified F1 riboprobe

(8) Wolframin-1-expressing neurons in the entorhinal cortex propagate tau to CA1 neurons and impair hippocampal memory in miceScience translational medicineSeptember 15, 2021Delpech JC, Pathak D, Varghese M, Kalavai SV, Hays EC, Hof PR, Johnson WE, Ikezu S, Medalla M, Luebke JI, et al.Roche Diagnostics GmbH, Mannheim, Germany) and Riboprobe In Vitro Transcription Systems (catalog no. P1420, Promega, Madison, WI) and purified using ProbeQuant G-50 microcolumns (catalog no. GE28–9034, GE Healthcare,

(9) Inhibition of colony stimulating factor 1 receptor corrects maternal inflammation-induced microglial and synaptic dysfunction and behavioral abnormalitiesMolecular psychiatryJune 1, 2021Ikezu S, Yeh H, Delpech JC, Woodbury ME, Van Enoo AA, Ruan Z, Sivakumaran S, You Y, Holland C, Guillamon-Vivancos T, et al.073, Roche Diagnostics GmbH, Mannheim, Germany) and Riboprobe In Vitro Transcription Systems (cat. no. P1420, Promega, Madison, WI) and purified using ProbeQuant G-50 micro columns (cat. no. GE28-9034, GE Healthcare,

(10) Rapid humoral immune responses are required for recovery from haemorrhagic fever with renal syndrome patientsEmerging Microbes & InfectionsOctober 21, 2020Yaoni Li et al.PET-20 vector and mRNA was synthesized following in vitro transcription using the Promega Kit (Cat.P1420). The concentration of the synthesized mRNA was measured using a NanoDrop ND-1000 spectrophotometer,

(11) 14-3-3 targets keratin intermediate filaments to mechanically sensitive cell-cell contactsMolecular biology of the cellApril 15, 2020Mariani RA, Paranjpe S, Dobrowolski R, Weber GF.Fraser, 2003). RNA constructs and microinjection RNA was prepared via in vitro transcription (Promega, P1420). DNA or RNA was diluted to microinject concentrations of 200–500 pg in 5-nl pulses for the following

(12) Cellular DNA Topoisomerases Are Required for the Synthesis of Hepatitis B Virus Covalently Closed Circular DNAJournal of virologyJune 1, 2019Sheraz M, Cheng J, Tang L, Chang J, Guo JT.in vitro transcription of the linearized recombinant plasmid with RiboProbe System-SP6 (catalog no. P1420; Promega) and used for Southern blot hybridization of human mtDNA. An mtDNA fragment of 7,366 nucleotides

(13) Regulation of HIV-1 Gag-Pol Expression by Shiftless, an Inhibitor of Programmed -1 Ribosomal FrameshiftingCellJanuary 24, 2019Wang X, Xuan Y, Han Y, Ding X, Ye K, Yang F, Gao P, Goff SP, Gao G.Dual-Luciferase® Reporter Assay System,Promega,Cat# E1910 T7 Riboprobe® Systems,Promega,Cat# P1440 Sp6 Riboprobe® Systems,Promega,Cat# P1420 SuperReal PreMix Plus (SYBR Green),Tiangen,Cat# Fp205-02 CD14 Microbeads,MACS Miltenyi Biotec,130-050-201 Glutathione Sepharose 4B

(14) Tissue stiffening coordinates morphogenesis by triggering collective cell migration in vivoNatureFebruary 14, 2018Elias H. Barriga, Kristian Franze, Guillaume Charras, Roberto MayorThe riboprobe against snail2 was transcribed with a Riboprobe® in vitro Transcription System (Promega P1420). All animal experiments were approved by the Biological Service Unit at University College London and

(15) Depletion of microglia and inhibition of exosome synthesis halt tau propagationNature neuroscienceNovember 1, 2015Asai H, Ikezu S, Tsunoda S, Medalla M, Luebke J, Haydar T, Wolozin B, Butovsky O, Kügler S, Ikezu T.073, Roche Diagnostics GmbH, Mannheim, Germany) and Riboprobe In Vitro Transcription Systems (cat. no. P1420, Promega, Madison, WI) and purified using ProbeQuant G-50 micro columns (cat. no. GE28-9034, GE Healthcare,

(16) Circadian genes, xBmal1 and xNocturnin, modulate the timing and differentiation of somites in Xenopus laevisPloS oneJanuary 1, 2014Curran KL, Allen L, Porter BB, Dodge J, Lope C, Willadsen G, Fisher R, Johnson N, Campbell E, VonBergen B, et al.linearized (Not1) xBMal1-CS2+, xNocturnin-CS2+, GFP-CS2+ was performed using Riboprobe system SP6 (Promega P1420). RNA levels were quantified using UV spectrophotometry (Nanodrop 2000c) and gel electrophoresis. We

(17) Diffuse brain injury induces local expression of Na +/ myo-inositol cotransporter in the rat brainMolecular Brain ResearchOctober 10, 2000Takashi Ueda, Akira Iwata, Hiroaki Komatsu, Noritaka Aihara, Kazuo Yamada, Shinya Ugawa, Shoichi Shimada

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