I-CeuI from New England Biolabs

Product Specs
ItemI-CeuI
CompanyNew England Biolabs
Price $80.00 Inquire
Catalog NumberR0699S
Quantity500 units
Concentration5,000 units/ml
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

TAACTATAACGGTCCTAA/GGTAGCGA

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Fortilin deficiency induces anti-atherosclerotic phenotypes in macrophages and protects hypercholesterolemic mice against atherosclerosisCommunications biologyJuly 11, 2025Wanachottrakul N, Pinkaew D, Mukherjee S, Chunhacha P, Nakashima M, Brockman AA, Pal U, Halim HB, Wei Y, Tanaka L, et al.verified the integrity of the construct by extensive sequencing, linealized the vector using I-CeuI (R0699S, New England BioLabs, Ipswich, MA, USA), and electroporated it into C57BL6 embryonic stem cells (ESCs).

(2) Combinatorial mapping of E3 ubiquitin ligases to their target substratesMol CellFebruary 20, 2025Chase C. Suiter, Diego Calderon, David S. Lee, Melodie Chiu, Shruti Jain, Florence M. Chardon, Choli Lee, Riza M. Daza, Cole Trapnell, Ning Zheng, et al."DNA Polymerase I, Large (Klenow) Fragment",New England Biolabs,M0210S I-SceI,New England Biolabs,R0694S I-CeuI,New England Biolabs,R0699S rCutSmart Buffer,New England Biolabs,B6004S "Recombinant Albumin, Molecular Biology Grade",New England Biolabs

(3) STK19 positions TFIIH for cell-free transcription-coupled DNA repairCellDecember 12, 2024Tycho E.T. Mevissen, Maximilian Kümmecke, Ernst W. Schmid, Lucas Farnung, Johannes C. WalterR0532S EcoRI-HF,NEB,Cat# R3101S KpnI-HF,NEB,Cat# R3142S XhoI,NEB,Cat# R0146S AflII,NEB,Cat# R0520S I-CeuI,NEB,Cat# R0699S NEBuilder HiFi DNA Assembly Master Mix,NEB,Cat# E2621L Q5 Hot Start High-Fidelity 2X Master Mix,NEB,Cat#

(4) MultiBac System-based Purification and Biophysical Characterization of Human Myosin-7aJournal of Visualized ExperimentsAugust 23, 2024Marvin Wright, Shayna Redford, Jacob Vehar, Kevin C. Courtney, Neil Billington, Rong LiuSolution,Gibco,15710–064,10 mg/mL in distilled water Glycerol,Invitrogen,15514–011, I-CeuI Enzyme,New England Biolabs,R0699S, In-Fusion Snap Assembly Master Mix,TaKaRa,638948, IPTG,Thermo Fisher,15529019, Isopropanol,Fisher

(5) Primase promotes the competition between transcription and replication on the same template strand resulting in DNA damageNature CommunicationsJanuary 2, 2024Weifeng Zhang, Zhuo Yang, Wenjie Wang, Qianwen Sunμl 1x CutSmart (NEB, B7204S) at 37 °C. The agarose pieces were then incubated with 3 μl I-CeuI (NEB, R0699S) in 150 μl 1x CutSmart Buffer at 37 °C for 12 h and washed 3 times with 1x TE buffer. Then the T7 ligation

(6) A Lac Repressor-Inducible Baculovirus Expression Vector for Controlling Adeno-Associated Virus Capsid RatiosVirusesDecember 28, 2023Jeffrey Slack, Christopher Nguyen, Amanda Ibe-Enwoligation steps, which are described as follows. VoyBac1.1 was linearized with the I-CeuI HEN enzyme (#R0699L, New England Biolabs) and then ligated to a synthetically made (GenScript, Piscataway, NJ, USA) I-Ceu-LacO-p10-LacO-VP1-I-CeuI

(7) Chimpanzee adenovirus-mediated multiple gene therapy for age-related macular degenerationiScienceOctober 20, 2023Selena Wei-Zhang, Bohao Cui, Man Xing, Jiaojiao Liu, Yingying Guo, Kai He, Tinghui Bai, Xue Dong, Yi Lei, Wei Zhou, et al.,New England Biolabs,Cat#R0629S PI-SceI,New England Biolabs,Cat#R0696S I-CeuI,New England Biolabs,Cat#R0699S PacI,New England Biolabs,Cat#R0547L ApaI,New England Biolabs,Cat#R0114S MfeI-HF,New England Biolabs,Cat#R3589L XhoI,New England Biolabs

(8) Strand asymmetry influences mismatch resolution during a single-strand annealingGenome BiologyDecember 1, 2022Victoria O. Pokusaeva, ArÃ¡nzazu Rosado Diez, Lorena Espinar, Albert TorellÃ³ PÃ©rez, Guillaume J. Filionthis, non-integrated plasmids were cut by 2 h digestion at 37 °C with I-CeuI restriction enzyme (NEB, R0699S) in a total volume of 70 ul followed by 20 min heat inactivation at 65 °C. All enzymatic reactions were

(9) Systematic analysis of intrinsic enhancer-promoter compatibility in the mouse genomeMolecular CellJuly 7, 2022Miguel Martinez-Ara, Federico Comoglio, Joris van Arensbergen, Bas van Steenselbrief, the combination of barcode and element(s) was excised from the plasmid by digestion with I-CeuI(#R0699S, NEB); this fragment was circularised; remaining linear fragments were destroyed; and circular fragments

(10) MegaGate: A toxin-less gateway molecular cloning toolSTAR ProtocolsDecember 17, 2021Christian Kramme, Alexandru M. Plesa, Helen H. Wang, Bennett Wolf, Merrick Pierson Smela, Xiaoge Guo, Richie E. Kohman, Pranam Chatterjee, George M. Church11789020 I-SceI (5U/ ul),New England Biolabs,Cat: R0694 I-CeuI (5U/ ul),New England Biolabs,Cat: R0699 10× CutSmart Buffer,New England Biolabs,Cat: B7204 T5 Exonuclease,New England Biolabs,Cat: M0663 Gibson Assembly Master Mix

(11) An integrated pipeline for mammalian genetic screeningCell Reports MethodsOctober 25, 2021Christian Kramme, Alexandru M. Plesa, Helen H. Wang, Bennett Wolf, Merrick Pierson Smela, Xiaoge Guo, Richie E. Kohman, Pranam Chatterjee, George M. Church11,791,020)•1 μL (5U) of the MegaNuclease I-SceI (NEB R0694)•1 μL (5U) of the MegaNuclease I-CeuI (NEB #R0699)•5 μL of 10X CutSmart Buffer (NEB B7204).•Nuclease free water was then utilized to bring the reaction

(12) A standard for near-scarless plasmid construction using reusable DNA partsNature CommunicationsDecember 1, 2019Xiaoqiang Ma, Hong Liang, Xiaoyi Cui, Yurou Liu, Hongyuan Lu, Wenbo Ning, Nga Yu Poon, Benjamin Ho, Kang ZhouRE-digestion solution used in Supplementary Fig. 11 contained 1 µL of each RE (NotI [R3189S, NEB] and I-CeuI [R0699S, NEB]), a certain quantity of plasmids (~1 µg), 5 µL of 10X CutSmart® Buffer (B7204S, NEB), and ultrapure

(13) Fortilin binds IRE1α and prevents ER stress from signaling apoptotic cell deathNature communicationsMay 26, 2017Pinkaew D, Chattopadhyay A, King MD, Chunhacha P, Liu Z, Stevenson HL, Chen Y, Sinthujaroen P, McDougal OM, Fujise K.sequencing to verify the integrity of the construct, the targeting vector was linealized by I-CeuI (R0699S, New England BioLabs, Ipswich, MA, USA) and electroporated into C57BL6 embryonic stem cells (ESCs).

(14) Genome-wide mapping of autonomous promoter activity in human cellsNature BiotechnologyFebruary 1, 2017Joris van Arensbergen, Vincent D. FitzPatrick, Marcel de Haas, Ludo Pagie, Jasper Sluimer, Harmen J. Bussemaker, Bas van Steenselassociate the barcodes with the linked genomic fragments, we digested 4 μg SuRE library with I-CeuI (#R0699S; NEB), followed by magnetic bead purification (1:1 ratio beads:DNA solution). Of this, 2 μg was self-ligated

(15) An efficient method of directly cloning chimpanzee adenovirus as a vaccine vectorNature protocolsNovember 1, 2010Zhou D, Zhou X, Bian A, Li H, Chen H, Small JC, Li Y, Giles-Davis W, Xiang Z, Ertl HCJ.cat. no. V0269S) SwaI (New England Biolabs, cat. no. R0604S) I-CeuI (New England Biolabs, cat. no. R0699S) PI-SceI (New England Biolabs, cat. no. R0696S) SgfI (Promega, cat. no. R5104) Eco47III (Promega,

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I-CeuI

I-CeuI from New England Biolabs

Description

Sequence

New England Biolabs

Citations (15)

Reviews

I-CeuI from New England Biolabs

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