CytoStim™, human 130-092-172 from Miltenyi Biotec

(1) Reproducible detection of antigen-specific T cells and Tregs via standardized and automated activation-induced marker assay workflowsCell Reports MethodsJuly 20, 2026Torin Halvorson, Gabrielle Boucher, Daniel Yokosawa, Jessica Qing Huang, Rosa Garcia, Lieke Sanderink, Lan Chen, Lorraine Liu, J. Ernesto Fajardo-Despaigne, Jasper Halvorson, et al.recombinant proteins","Chemicals, peptides, and recombinant proteins" ,, "CytoStim, human",Miltenyi,Cat#130-092-172 "PepTivator CMV pp65, human",Miltenyi,Cat#130-093-438 PepTivator SARS-CoV-2 Prot_S_complete,Miltenyi,Cat#130-127-951 INFANRIX

(2) An In Vitro test battery using human whole blood for immunotoxicity hazard identification: Proof of concept studies with dexamethasone and benzo(a)pyreneToxicologyAugust 1, 2025Victor J. Johnson, Michael I. Luster, Amber Edwards, Michael Kashon, Gary R. Burleson, Florence G. Burleson, Dori R. Germolechuman antibody cocktail that binds the T-cell receptor and crosslinks to MHC; Miltenyi Biotec, Cat# 130-092-172), 3) 50 μL of SARS-CoV-2 peptide pool, or 4) 50 μL of influenza peptide pool (see the section 2.3 for

(3) No evidence of immune exhaustion after repeated SARS-CoV-2 vaccination in vulnerable and healthy populationsNat CommunJune 5, 2025Jenna M. Benoit, Jessica A. Breznik, Ying Wu, Allison Kennedy, Li-Min Liu, Braeden Cowbrough, Barbara Baker, Megan Hagerman, Catherine M. Andary, Maha Mushtaha, et al.and used to stimulate PBMCs at a final concentration of 1 µg/mL for 44 h at 37 °C/5% CO2. Cytostim (#130-092-172, Miltenyi Biotec) was used as a positive stimulation control for each sample at 0.25 µL per well, and

(4) Human LY9 governs CD4 + T cell IFN-γ immunity to Mycobacterium tuberculosisScience ImmunologyMay 30, 2025Masato Ogishi, Julia Puchan, Rui Yang, Andrés Augusto Arias, Ji Eun Han, Tina Nguyen, Rebeca Gutiérrez-Cózar, Clément Conil, Yoann Seeleuthner, Darawan Rinchai, et al.non-stimulated or were stimulated with filtered HKMTb (InVivoGen, 400 μg/mL), CytoStim (Miltenyi Biotec, Cat: 130–092-172, 1:100), HKMTb + CytoStim, ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (STEMCELL, 1:100), or Cell

(5) Distinct HLA Haplotypes Are Associated With an Altered Strength of SARS-CoV-2-Specific T-Cell Responses and Unfavorable Disease CoursesEur J ImmunolApril 21, 2025C. Dörnte, A. Datsi, V. Traska, J. Kostyra, M. Wagner, O. Brauns, C. Lamsfuß, H. Winkels, V. Balz, J. Enczmann, et al.unstimulated control, as well as two positive controls stimulated with CytoStim (Miltenyi Biotec, cat. no. 130‐092‐172) and SARS‐CoV‐2 PepTivators (PepTivator SARS‐CoV‐2 Prot_S Complete, cat. no. 130‐127‐951; PepTivator SARS‐CoV‐2 Prot_M

(6) T-Cell Responses to COVID-19 Vaccines and Breakthrough Infection in People Living with HIV Receiving Antiretroviral TherapyVirusesApril 24, 2024Sneha Datwani, Rebecca Kalikawe, Rachel Waterworth, Francis M. Mwimanzi, Richard Liang, Yurou Sang, Hope R. Lapointe, Peter K. Cheung, Fredrick Harrison Omondi, Maggie C. Duncan, et al.media only (no peptide) as a negative control or two µg/mL of a CytoStim reagent (Miltenyi Biotec, Cat#130-092-172) as a positive control. Following stimulation, the cells were labeled with CD8-APC/Cyanine7 (Biolegend,

(7) Identification of immune subsets with distinct lectin binding signatures using multi-parameter flow cytometry: correlations with disease activity in systemic lupus erythematosusFrontiers in immunologyJanuary 1, 2024Szabó E, Faragó A, Bodor G, Gémes N, Puskás LG, Kovács L, Szebeni GJ.supplemented with 10% FBS (200 μL/well). After two hours, the stimulating CytoStim (100x dilution, Cat. 130-092-172, Miltenyi Biotec, Auburn, USA) reagent was added in complete RPMI to the “activated state” samples along

(8) Dynamics of T-cell Responses Following COVID-19 mRNA Vaccination and Breakthrough Infection in Older AdultsPathogens and ImmunityNovember 17, 2023Sneha Datwani, Rebecca Kalikawe, Francis Mwimanzi, Sarah Speckmaier, Richard Liang, Yurou Sang, Rachel Waterworth, Fatima Yaseen, Hope R. Lapointe, Evan Barad, et al.with DMSO only (no peptide) as a negative control and 2 µg/mL Cytostim reagent (Miltenyi Biotec, Cat#130-092-172) as a positive control. Following stimulation, cells were labeled with CD8-APC/Cyanine7 (Biolegend,

(9) Cytotoxicity of WT1-reactive T cells against Wilms tumor: An implication for antigen-specific adoptive immunotherapyBioImpactsJune 12, 2023Seyed Mostafa Monzavi, Amir Ali Hamidieh, Mohammad Vasei, Jafar Ai, Naser Ahmadbeigi, Hamid Arshadi, Samad Muhammadnejad, Abdol-Mohammad Kajbafzadeh#130-054-201), LS columns (Miltenyi Biotec, Germany, #130-042-401), CytoStimTM (Miltenyi Biotec, Germany, #130-092-172), TexMACSTM medium (Miltenyi Biotec, Germany, #130-097-196), recombinant human IL-2 (Miltenyi Biotec,

(10) Immunological response after SARS-CoV-2 infection and mRNA vaccines in patients with myasthenia gravis treated with RituximabNeuromuscular DisordersMarch 1, 2023Valentina Damato, Gregorio Spagni, Gabriele Monte, Letizia Scandiffio, Paola Cavalcante, Nicole Zampetti, Marco Fossati, Silvia Falso, Renato Mantegazza, Alessandra Battaglia, et al.control (without antigen) and a positive control (polyclonally stimulated with CytoStim, Miltenyi Biotec, 130–092–172), were included. After 2 h of incubation at 37 °C (5% CO2), Brefeldin A (1:1000, Sigma Aldrich) was

(11) A protocol to detect human CD4+ T cell extracellular traps using scanning electron microscopySTAR ProtocolsMarch 1, 2023Nam Phuong N. Nguyen, Nicole Chizara Oparaugo, Kelsey Ouyang, George W. AgakSciences,20816-12-0 "Ethyl alcohol, anhydrous",Electron Microscopy Sciences,64-17-5 CytoStim,Miltenyi Biotec,130-092-172 RPMI 1640,Miltenyi Biotec,130-091-440 "Human AB serum, Gemini Bio",Fisher Scientific,50-753-3010 autoMACS Rinsing Solution

(12) Antigen presentation safeguards the integrity of the hematopoietic stem cell poolCell Stem CellMay 5, 2022Pablo Hernández-Malmierca, Dominik Vonficht, Alexandra Schnell, Hannah J. Uckelmann, Alina Bollhagen, Mohamed A.A. Mahmoud, Sophie-Luise Landua, Elise van der Salm, Christine L. Trautmann, Simon Raffel, et al.Violet,ThermoFisher,Cat#C34557 Dynabeads Mouse T-Activator,ThermoFisher,Cat#11452D CytoStim,Miltenyi,Cat#130-092-172 PepMix CEFX Ultra SuperStim MHC-II Subset Pool,JPT,Cat#PM-CEFX-3 Mouse TPO,PreproTech,Cat#315-14 Mouse

(13) Fc-based Duokines: dual-acting costimulatory molecules comprising TNFSF ligands in the single-chain format fused to a heterodimerizing Fc (scDk-Fc)OncoimmunologyJanuary 1, 2022Aschmoneit N, Kocher K, Siegemund M, Lutz MS, Kühl L, Seifert O, Kontermann RE.CellTrace™ Far Red Cell Proliferation Kit (C34564) were purchased from Thermo Fisher Scientific. CytoStim (130–092-172) was purchased from Miltenyi Biotec. Stable transfected HT1080-CD40, HT1080-CD27, HT1080-OX40 and HT1080-4-1BB

(14) Long-Lasting Immunity Against SARS-CoV-2: Dream or Reality?Frontiers in medicineJanuary 1, 2021Gussarow D, Bonifacius A, Cossmann A, Stankov MV, Mausberg P, Tischer-Zimmermann S, Gödecke N, Kalinke U, Behrens GMN, Blasczyk R, et al.ng/ml, #P1585) and ionomycin (500 ng/ml, Sigma Aldrich, #I9657) as well as CytoStim (Miltenyi Biotec, #130-092-172) served as a positive control whereas unstimulated PBMCs were used as a negative control. After one

(15) Identification of hnRNP-A1 as a pharmacodynamic biomarker of type I PRMT inhibition in blood and tumor tissuesScientific reportsDecember 17, 2020Noto PB, Sikorski TW, Zappacosta F, Wagner CD, Montes de Oca R, Szapacs ME, Annan RS, Liu Y, McHugh CF, Mohammad HP, et al.concentrations either in the absence (non stimulated) or presence of human CytoSTIM (TCR-activated) (Miltenyi, #130-092-172) at a final concentration of 1 µg/mL for 72 h. Jurkat and Toledo cells (ATCC, TIB-152 and CRL-2631,

(16) Transcriptional and Functional Analysis of CD1c⁺ Human Dendritic Cells Identifies a CD163⁺ Subset Priming CD8⁺CD103⁺ T CellsImmunityAugust 18, 2020Bourdely P, Anselmi G, Vaivode K, Ramos RN, Missolo-Koussou Y, Hidalgo S, Tosselo J, Nuñez N, Richer W, Vincent-Salomon A, et al.and 105 cells were plated on top of mononuclear phagocytes in presence of CytoStim (Miltenyi, Cat# 130-092-172), according to manufacturer’s instructions. For mixed leukocyte reaction (MLR) experiments, isolated

(17) Unsupervised capture and profiling of rare immune cells using multi-directional magnetic ratchetingLab on a chipAugust 7, 2018Murray C, Miwa H, Dhar M, Park DE, Pao E, Martinez J, Kaanumale S, Loghin E, Graf J, Raddassi K, et al.N-Acetyl-L-cysteine (Cat No. A9165-25G, Sigma Aldrich, USA). T cells were activated using CytoStim (Cat No. 130-092-172, Miltenyi Biotec, Bergisch Gladbach, Germany). PBMCs in serum containing culture media at a cell density

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CytoStim™, human from Miltenyi Biotec

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CytoStim™, human from Miltenyi Biotec

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