Description
Skeletal muscle regenerates by the proliferation of mononuclear myogenic precursor cells called myoblasts that ultimately fuse and become incorporated into multinucleated myotubes. The myotubes then later mature into myofibers. This process occurs during the embryonic histogenesis of muscle, in postnatal muscle regeneration in response to injury, or in diseases such as Duchenne muscular dystrophy. The fusion of myoblasts is specific to skeletal muscle and myoblasts that do not form muscle fibers differentiate into satellite cells. Skeletal muscle myoblasts express the FGF receptor [1] and IGF expression is increased during myoblast differentiation in culture [2, 3]. The human skeletal muscle myoblast culture is a convenient in vitro model for the study of cellular development and differentiation, insulin metabolism and tissue repair. HSkMM from ScienCell Research Laboratories are isolated from human muscle of the pectoral girdle. HSkMM are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HSkMM are characterized by immunofluorescence with antibodies specific to myosin, actin and actinin. HSkMM are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HSkMM are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research laboratories. Recommended Medium: It is recommended to use Skeletal Muscle Cell Medium (SkMCM, Cat. #3501) for the culturing of HSkMM in vitro