MCF 10A from ATCC

Product Specs
ItemMCF 10A
CompanyATCC
Price $431.00Supplier Page View Company Product Page
Catalog NumberCRL-10317
TypeCell Biology
SpeciesHomo sapiens
TissueMammary gland, Breast
Cell LineMCF 10A
Validated Species ReactivityHuman (Hu)
Tissue (standardized)Breast
Cell Typeepithelial cell
Age36 years
DiseaseFibrocystic Disease
ApplicationsThis line is suitable as a transfection host

Specifications/Features

Post freeze viability ≥ 50%

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ATCC

10801 University Boulevard

Manassas, Virginia 20110-2209

United States

Phone: (703) 365-2700
Fax: (703) 365-2701

Company Profile

Website: http://www.atcc.org

(1) Passive nuclear transport deviates from Fickian behavior in prostate and breast cell typesNucleusDecember 31, 2026Nicholas R. Scott, Alexander J. Lin, Brian Belardi, Sapun H. Parekh11,875–093) with 10% FBS and no antibiotics. MCF-10A (female breast fibrocystic line, ATCC catalog # CRL-10317, RRID:CVCL0598) was grown in DMEM/F12 (thermofisher catalog # 11,320,033) with 5% horse serum, no antibiotics,

(2) Promotion of breast cancer by the DNPH1 enzymeiScienceApril 17, 2026Sangphil Oh, Yuan Sui, Sook Shin, Ralf Janknecht,Experimental models: cell lines,Experimental models: cell lines ,, Mouse: 4T1,ATCC,CRL-2539 Human: MCF10A,ATCC,CRL-10317 Human: SKBR3,ATCC,HTB-30 Human: MDA-MB-453,ATCC,HTB-131 Human: MCF7,ATCC,HTB-22 Human: T47D,ATCC,HTB-133 Human:

(3) Regulatory BC200 RNA promotes breast carcinogenesis by repressing BRCA1 gene expressioniScienceApril 17, 2026Valerio Berardi, Ilham A. Muslimov, Dan Sun, Giuseppe Militello, Prem Premsrirut, Henri TiedgeCell lines,Experimental models: Cell lines,Experimental models: Cell lines ,, MCF-10A cells,ATCC,CRL-10317; RRID: CVCL_0598 MCF-7 cells,ATCC,HTB-22; RRID: CVCL_0031 SUM229PE cells,BIOIVT,SUM229PE; RRID: CVCL_5594 ,, Oligonucleotides,Oligonucleotides,Oligonucleotides ,, shRNAs

(4) Anti-Cancer Effect of a New 5-FU Derivative Containing Triazole-Bearing Mannose (5-FUD-MAN) Against Human Breast Cancer Cells Through LC3B-Mediated Cell DeathJ Biochem Mol ToxicolMarch 29, 2026Ebru Şanci, Azada Aliyeva, Buket Bakan, Mustafa Özkaraca, Erkan Halay, Kadir Ay, Tamer Karayildirim, N. Ulku Karabay YavasogluFigure 2. 2.2 Cell Culture and Cytotoxicity Assay MCF‐10A (Human breast epithelial cell line, ATCC‐CRL‐10317) and MCF‐7 (Human breast epithelial cancer cell line, ATCC‐HTB‐22) cells were cultured in Dulbecco′s

(5) CXCL12-mediated T cell infiltration drives breast cancer metastasisClinical and Experimental MedicineMarch 28, 2026Xianfu Liu, Chunchun Chen, Lu Liu, Jingwei Tang, Hao Zhang, Gongsheng Jin, Yansong Chenfor breast cancer metastasis. Materials and methods Cell culture The MCF-10 A cell line (ATCC, CRL-10317) was cultured with MEGM™ Mammary Epithelial Cell Growth Medium. The MDA-MB-231 cell line (ATCC, CRM-HTB-26)

(6) The ATC12 small molecule inhibits the Aurora-A/TPX2 interaction and impairs the proliferation of breast cancer cellsCell Death & DiseaseMarch 24, 2026Dalila Boi, Giulia Fianco, Federica Polverino, Francesco Fiorentino, Anna Mastrangelo, Simone Rossi, Elisabetta Rubini, Serena Rosignoli, Francesca Troilo, Maria Rosaria Antonelli, et al.the American Type Culture Collection (ATCC, Manassas, VA, USA; MCF7 HTB-22, MDA-MB-231 HTB-26, MCF10A CRL-10317). U2OS were from ATCC (HTB-96). Cells were grown at 37 °C and 5% CO2 in complete DMEM (U2OS and MCF7 cells

(7) Discovery of Two Novel Pyrazole Derivatives as Anticancer Agents Targeting Tubulin Polymerization and MAPK Signaling PathwaysOncology ResearchMarch 23, 2026Denisse A. Gutierrez, Elisa Robles-Escajeda, Jose A. Lopez-Saenz, Robert A. Kirken, Edgar A. Borrego, Ana P. Betancourt, Soumya Nair, Sourav Roy, Armando Varela-Ramirez, Renato J. Aguilerawith 15% FBS for MiNo and 20% for Jeko-1 and HL-60. Lastly, PC-3 (ATCC, CRL-1435), and MCF10A (ATCC, CRL-10317), cell lines were grown in Dulbecco’s Modified Eagle’s Medium-F12 (DMEM-F12) media (Corning, MT10090CV)

(8) Porphyrin-coated gold nanoparticles associated with X-rays and proton therapy in the treatment of triple-negative breast cancerNanoscale AdvancesMarch 17, 2026Camila Ramos Silva, Guillaume Berionni, Martha Simões Ribeiro, Anne-Catherine Heuskinwith 10% FBS, 100 U mL−1 penicillin, and 100 µg mL−1 streptomycin (Merck, Belgium). MCF10A cells (ATCC CRL-10317) were grown in DMEM/F-12 (Merck, Belgium) with special supplementation: 5% horse serum (Invitrogen,

(9) Tumour specific HORMAD1 expression perturbs mitotic arrest and drives sensitivity to mitotic kinase inhibitorsNat CommunMarch 11, 2026Callum Walker, Gabriel Kollarovic, Daniel Weekes, Jennifer Trendell, Ricarda M. Hoffmann, Alexia Martin, Riccardo Ferro, Blanca Navarro-Llinas, Luke Hitchen, Mercedes Pardo Calvo, et al.−80 °C. Cell culture Cell lines obtained from ATCC: RPE1 hTERT (CRL-4000), HeLa (CCL-2), MCF10A (CRL-10317), MDA-MB-436 (HTB-130) NCI-H1299 (CRL-5803). Cell lines obtained from BioIVT/Asterand: SUM159PT (HUMANSUM-0003006),

(10) Development of a Prognostic Stratification Model and Identification of BDH1 as an Oncoprotein in Breast Cancer Based on Subcluster-Specific Markers of B-Cell SubsetsBreast Cancer (Dove Med Press)March 10, 2026Shujuan Kang, Zhongxin Li, Chao Lv, Fenghua Zhangvisualized using the PyMOL software. Cell Culture Human immortalized mammary gland cell lines MCF-10A (CRL-10317) and BC cell lines BT-474 (HTB-20), MCF-7 (HTB-22), MDA-MB-231 (HTB-26), BT-549 (HTB-122), T-47D (HTB-133),

(11) Protocol to identify mechanosensitive nuclear proteins using tunable actomyosin contractility and proximity biotinylation in mammalian cellsSTAR ProtocolsMarch 1, 2026Pei-Li Tseng, Weiwei Sun, Jiawei Li, Mark O. Collins, Kai S. Erdmann,Experimental models: Cell lines ,, Flp-In T-REX HEK293,Thermo Fisher Scientific,RRID: CVCL_U427 MCF10A,ATCC,#CRL-10317; RRID: CVCL_0598 NIH3T3,ATCC,#CRL-1658; RRID: CVCL_0594 HEK293-tet-RhoA-TurboID,This study,N/A ,, "Chemicals,

(12) MiR-5100 promotes the malignant phenotype of triple-negative breast cancer cells by targeting SMAD4 and predicts poor prognosisWorld Journal of Surgical OncologyFebruary 28, 2026Yaxi Song, Haiyan Wei, Jing Zhai, Peng Dai, Lei Hanhigh-glucose DMEM supplemented with 10% FBS, whereas the normal breast epithelial cell line MCF-10 A (ATCC, CRL-10317) was maintained in standard DMEM with 10% FBS. All cells were maintained at 37 °C in an incubator with

(13) Discovery of a Potent, Selective, and In Vivo Efficacious Covalent Inhibitor for Lysine Methyltransferase SETD8Journal of Medicinal ChemistryFebruary 26, 2026He Chen, Rudra Prasad Dutta, Zhizhong Li, Yue Zhong, Anqi Ma, Kwang-Su Park, Jithesh Kottur, Alison Park, Nicolas Babault, Ke Wang, et al.Parekh’s lab. PNT2 normal cells were purchased from Sigma-Aldrich (cat# 95012613), and MCF10A cells (CRL-10317) were purchased from ATCC (American Type Culture Collection). MM.1S, KMS20, EJM, PBMC and PNT2 cells

(14) The nuclease EXO1 promotes genomic instability by degrading nascent DNA in BRCA-proficient cellsNature CommunicationsFebruary 25, 2026Alexandra Nusawardhana, Claudia M. Nicolae, George-Lucian MoldovanHTB-133), MDA-MB-231 (ATCC HTB-26), MCF7 (ATCC HTB-22), MDA-MB-468 (ATCC HTB-132), and MCF10A (ATCC CRL-10317) cells, obtained from ATCC, were grown in Dulbecco’s modified Eagle’s media (DMEM) supplemented with

(15) SFPQ directs histone H3.3 deposition to R-loops in DNA repeats to protect genome stabilityNature CommunicationsFebruary 24, 2026Alessandro Ferrando, Michele Giaquinto, Luisa M. R. Napolitano, Giulia Canarutto, Alessandro Framarini, Alice Gambelli, Pamela Veneziano Broccia, Annie Zappone, Eleonora Petti, Chiara Boncristiani, et al.(Euroclone), 1% L-glutamine (Gibco), 1% penicillin/streptomycin (Gibco). MCF 10A (breast epithelial, ATCC CRL-10317) were cultured in high glucose Dulbecco’s modified Eagle’s (DMEM) medium and HAMS F12 (1:1 ratio, Euroclone),

(16) Lamin B receptor upregulation in metastatic melanoma causes nuclear envelope fragility in confined migration during cancer invasionProceedings of the National Academy of SciencesFebruary 24, 2026Michelle A. Baird, Cayla E. Jewett, Daniela A. Malide, Lisa Kratz, Alexander X. Cartagena-Rivera, Robert S. Fischer, Clare M. Watermanmedia (ATCC, PCS-200-030), supplemented with melanocyte growth kit (ATCC, PCS-200-041). MCF10A (ATCC, CRL-10317), PC-3 (ATCC, CRL-1435), HEK293FT (ATCC, CRL-3216), and T47D (NIH, NCI-60) cells were all cultured in

(17) Functional effects of EpCAM N-glycosylation in MDA-MB-468 breast cancer cellsScientific ReportsFebruary 20, 2026Nicole M. Jenkinson, Harsh Oza, Kevin J. Yarema, Kristine Glundenon-malignant immortalized human breast cell lines, MCF10A (female, American Type Culture Collection/ATCC #CRL-10317, Manassas, VA, USA) and MCF12A (female, ATCC #CRL-3598), four immortalized human breast cancer cell

(18) The microcephaly-associated protein YIPF5 differentially regulates ER exportiScienceFebruary 20, 2026Francesca Bruno, Mihaela Anitei, Domenico Di Fraia, William Durso, Therese Dau, Emilio Cirri, Mara Sannai, Christina Valkova, Julija Maldutyte, Elizabeth A. Miller, et al.10270106), and 1% penicillin-streptomycin (Thermo Fisher Scientific, cat. no. 15140122). MCF10A cells (ATCC CRL-10317) were maintained in DMEM/F-12 supplemented with 5% horse serum (Thermo Fisher Scientific, cat. no. 16050122),

(19) Precise control of transcription condensates across S phase balances linker histone expression with DNA replication, ensuring genome stabilityMolecular CellFebruary 19, 2026Carlos Origel Marmolejo, Celina Sanchez, Erin Helms, Melissa J. McEvoy, Juyoung Lee, Marcel Werner, Paige Roberts, Stephan Hamperl, Joshua C. Saldivarisolated in 1984 from the mammary gland of a 36-year-old Caucasian female with fibrocystic breasts (ATCC, CRL-10317). MCF10A cells were cultured in DMEM:F12 (Thermo Fisher Scientific, 11320082) base medium, supplemented

(20) Dual Targeting of FAP-Directed Nanoparticles and FRα-Specific CAR-T Cells Induces Additive Anti-Tumor Effects in Triple-Negative Breast CancerInt J Biol SciFebruary 18, 2026Tanva Thongkleang, Suyanee Thongchot, Kamonlatth Rodponthukwaji, Piriya Luangwattananun, Kwanruthai Tadpetch, Pa-Thai Yenchitsomanus, Peti Thuwajit, Primana Punnakitikashem, Chanitra ThuwajitUniversity (COA no. Si 182/2023). Cell lines and cell culture Normal mammary epithelial MCF-10A cells (#CRL-10317, ATCC) were cultured in Dulbecco's Modified Eagle's Medium/F12 (DMEM/F12; Gibco). The medium was supplemented

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