Description
Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies as they are one of the easiest types of cells to grow in culture. Their durability also makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. There is evidence that fibroblasts in various parts of the body are intrinsically different [1]. Fibroblasts within tissues are exposed to a dynamic mechanical environment, which influences the structure integrity of both healthy and healing soft tissue. Fibroblasts secrete a non-rigid extracellular matrix that is rich in type I and/or type III collagen [2]. Human bladder stromal fibroblasts (HBdSF) also secrete FGF-7, which interact primarily with bladder epithelial cells [3]. FGF-7 secreted by HBdSF is essential for stimulating the formation of a multilayered stratified bladder epithelium [4]. HBdSF from ScienCell Research Laboratories are isolated from human bladder tissue. HBdSF are cryopreserved at passage one and are delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HBdSF are characterized by immunofluorescence with antibodies specific to fibronectin. HBdSF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HBdSF are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories. Recommended Medium: It is recommended to use Fibroblast Medium (FM, Cat. #2301) for the culturing of HBdSF in vitro