HpyCH4V from New England Biolabs

Product Specs
ItemHpyCH4V
CompanyNew England Biolabs
Price $75.00 Inquire
Catalog NumberR0620S
Quantity100 units
Concentration5,000 units/ml
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

TG/CA

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Highly efficient chromatin conformation capture with post-enrichment in single cells by HiChewGenome BiolApril 27, 2026Zhichao Chen, Yeming Xie, Chen Zhang, Chen Tan, Fengying Ruan, Wenfang Chen, Meng Luo, Mei Guo, Zhe Xie, Huimin Cao, et al.and resuspended in 200 μL of the same buffer. For digestion, 10 μL of HpyCH4V enzyme (NEB, Cat. No. R0620L) was added, and the reaction was incubated at 37 °C with shaking at 1,000 rpm for 4 h. Aliquots were

(2) Topologically Associating Domains Define the 3D Genome Architecture of Mouse Totipotent-like Stem CellsJournal of Biological ChemistryFebruary 5, 2026Yu Fu, Wenyu Ma, Jin Han, Wenhui Zhang, Shenghu Zhang, Yuxiao Chen, Yue Qin, Junyang Li, Ziwei Zhai, Pengli Li, et al.quenched with 3% Triton X-100. The chromatin was digested with AluI (NEB, R0137L) and HpyCH4V (NEB, R0620L) at 37 °C overnight. A-tailing of the digestion ends was achieved by dATP (NEB, N0440S), Klenow Large

(3) Histone H3 lysine methyltransferase activities control compartmentalization of human centromeresbioRxivJuly 4, 2025Pragya Sidhwani, Jacob P. Schwartz, Kelsey A. Fryer, Aaron F. Straightmutations, we PCR’d the site of mutation and performed restriction digestion with HpyCH4V (NEB Cat#R0620S), which would only digest the unedited and not the edited allele. Validation of knockout cell lines

(4) Tri-omic single-cell mapping of the 3D epigenome and transcriptome in whole mouse brains throughout the lifespanNature MethodsMay 1, 2025Haoxi Chai, Xingyu Huang, Guangzhou Xiong, Jiaxiang Huang, Katarzyna Karolina Pels, Lingyun Meng, Jin Han, Dongmei Tang, Guanjing Pan, Liang Deng, et al.37° C for 20 min. Cells/nuclei were then in situ digested by AluI (NEB; R0137L) or AluI+HpyCH4V (NEB; R0620L) restriction enzymes at 37 °C for at least 2 h or overnight by adding 2 μl AluI/ 1 μl AluI + 1 μl HpyCH4V,

(5) Neuropeptide and serotonin co-transmission sets the activity pattern in the C. elegans egg-laying circuitCurrent BiologyOctober 21, 2024Allison Butt, Sara Van Damme, Emerson Santiago, Andrew Olson, Isabel Beets, Michael R. KoelleTransfection Reagent,Invitrogen,Cat#15338-500 spCas9 2NLS Nuclease,Synthego,NA HpyCH4V,New England BioLabs Inc,Cat#R0620 Experimental Models: Cell Lines,Experimental Models: Cell Lines,Experimental Models: Cell Lines CHO/mtAEQ/Gα16,PerkinElmer,ES-000-A24 Experimental Models

(6) Gene editing and reprogramming of human fibroblast cells (hFBs) to human pluripotent stem cells (hiPSCs)NeuromethodsJuly 14, 2024Zhe Zhang, Shinghua DingU151B QIAquick Gel Extraction kit,Qiagen,Cat# 28704 HpyCH4V restriction endonuclease,New England Biolabs,Cat# R0620 Dulbecco’s PBS (DPBS),Thermo Fisher Scientific,Cat# 14190 "Vitronectin, truncated recombinant human",Thermo Fisher Scientific

(7) Single duplex DNA sequencing with CODEC detects mutations with high sensitivityNature GeneticsMay 1, 2023Jin H. Bae, Ruolin Liu, Eugenia Roberts, Erica Nguyen, Shervin Tabrizi, Justin Rhoades, Timothy Blewett, Kan Xiong, Gregory Gydush, Douglas Shea, et al.65 °C for 30 min. We modified ddBTP-blocked ER/AT22 to start with fragmentation with HpyCH4V (NEB, R0620S) and AluI (NEB, R0137S; Extended Data Fig. 6b) in NEB CutSmart buffer at 37 °C for 15 min, purified

(8) ChIATAC is an efficient strategy for multi-omics mapping of 3D epigenomes from low-cell inputsNature communicationsJanuary 13, 2023Chai H, Tjong H, Li P, Liao W, Wang P, Wong CH, Ngan CY, Leonard WJ, Wei CL, Ruan Y.incubated at 37 °C for 20 min. Cells were then in situ digested by AluI (NEB; R0137L) or AluI+HpyCH4V (NEB; R0620L) restriction enzymes at 37 °C for at least 2 h or overnight by adding 2 μl AluI/ 1 μl AluI + 1 μl HpyCH4V,

(9) Reversible epigenetic alterations regulate class I HLA loss in prostate cancerCommunications biologySeptember 1, 2022Rodems TS, Heninger E, Stahlfeld CN, Gilsdorf CS, Carlson KN, Kircher MR, Singh A, Krueger TEG, Beebe DJ, Jarrard DF, et al.10 units/µL (New England Biolabs, Cat# R0137L) and HpyCH4V at 5 units/µL (New England Biolabs, Cat# R0620L)) in 20 µL reactions containing 1x CutSmart Buffer (New England Biolabs, Cat# B7204S) for 15 min at

(10) Single-cell measurement of higher-order 3D genome organization with scSPRITENature BiotechnologyJanuary 1, 2022Mary V. Arrastia, Joanna W. Jachowicz, Noah Ollikainen, Matthew S. Curtis, Charlotte Lai, Sofia A. Quinodoz, David A. Selck, Rustem F. Ismagilov, Mitchell Guttmansame instrument at 1,200 r.p.m. for 60 min at 37 °C. Lastly, 30 μl of 5,000 U ml−1 HpyCH4V (NEB, no. R0620L) was added to the same tube to allow for DNA to be digested in-nuclei. In-nuclei digestion was performed

(11) Somatic mutation landscapes at single-molecule resolutionNatureMay 20, 2021Federico Abascal et al.in: https://github.com/cancerit/NanoSeq https://github.com/fa8sanger/NanoSeq_Paper_Code Introduction Reagents NEBNext® Ultra™ II Q5® Master Mix (NEB: M0544L) HpyCH4V (NEB: R0620L) T4 DNA Ligase (NEB: M0202L) dATP Solution (NEB: N0440S) xGen CS Adapter - Tech Access (IDT: 1080799) CutSmart® Buffer

(12) Mapping the epigenomic and transcriptomic interplay during memory formation and recall in the hippocampal engram ensembleNature NeuroscienceDecember 1, 2020Asaf Marco et al.Life Technologies, 11205D). For the second restriction enzyme we used the 4-base cutter Hpych4V (10 U /per sample, R0620S, New England Biolabs, USA). After A-tailing reaction (see original protocol for details), samples were

(13) Anosmin1 Shuttles Fgf to Facilitate Its Diffusion, Increase Its Local Concentration, and Induce Sensory OrgansDevelopmental CellSeptember 1, 2018John Wang, Yandong Yin, Stephanie Lau, Jagadish Sankaran, Eli Rothenberg, Thorsten Wohland, Martin Meier-Schellersheim, Holger Knaut"Alexa Fluor 594 Carboxylic Acid, tris(triethylammonium) salt",Thermo Fisher,A33082 BsrI,New England Biolabs,R0527S Hpych4v,New England Biolabs,R0620L AciI,New England Biolabs,R0551S ApoI,New England Biolabs,R0566L Taqαi,New England Biolabs,R0149L Brefeldin

(14) Genome-wide mapping of autonomous promoter activity in human cellsNature BiotechnologyFebruary 1, 2017Joris van Arensbergen, Vincent D. FitzPatrick, Marcel de Haas, Ludo Pagie, Jasper Sluimer, Harmen J. Bussemaker, Bas van Steenseldigested for 1 hour with 10 units of frequent cutter Nla III (#R0125S; NEB) or 10 units of HpyCH4V (#R0620L; NEB), bead purified and self-ligated again in a final volume of 1 ml. This material was purified by

(15) In silico analysis identifies novel restriction enzyme combinations that expand reduced representation bisulfite sequencing CpG coverageBMC Research NotesDecember 1, 2014Daniel B Martinez-Arguelles, Sunghoon Lee, Vassilios Papadopoulosnumber AluI,AG|CT,37,CutSmart,80,No,R0137S BfaI,C|TAG,37,CutSmart,80,No,R0568S HaeIII,GG|CC,37,CutSmart,80,No,R0108S HpyCH4V,TG|CA,37,CutSmart,65,No,R0620S MluCI,|AATT,37,CutSmart,No,No,R0538S MseI,T|TAA,37,CutSmart,65,No,R0525S MspI,C|CGG,37,CutSmart,No,No,R0106S TaqI,T|CGA,65,CutSmart,80,dam,R0149S CviQI,G|TAC,25,NEB

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HpyCH4V

HpyCH4V from New England Biolabs

Description

Sequence

New England Biolabs

Citations (15)

Reviews

HpyCH4V from New England Biolabs

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