HpyCH4IV from New England Biolabs

Product Specs
ItemHpyCH4IV
CompanyNew England Biolabs
Price $75.00 Inquire
Catalog NumberR0619S
Quantity500 units
Concentration10,000 units/ml
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

A/CGT

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) A quantitative in vivo CRISPR-imaging platform identifies regulators of hyperplastic and hypertrophic adipose morphology in zebrafisheLifeApril 22, 2026Rebecca Wafer, Panna Tandon, James EN MinchinThe PCR product was then digested with the restriction enzyme HpyCH4IV (New England Biolabs, Cat. # R0619S). Additionally, KASP genotyping assays were designed for the ed116 and ed125 alleles (KASP on demand,

(2) Deaminase-Assisted Sequencing for the Identification of 5-glyceryl-methylcytosineBIO-PROTOCOLFebruary 20, 2026Bao-Dan He, Fan-Chen Wang, Jian-Huang XueRNase A/T1 mix (Thermo Fisher Scientific, catalog number: EN0551) 3. HpyCH4IV (TaiI) (NEB, catalog number: R0619S) 4. FastDigest SsiI (AciI) (Thermo Fisher Scientific, catalog number: FD1794) 5. 5-methyl-dCTP (2.5

(3) Identifying Differentially Methylated Sites for Methylation-Sensitive qPCR-based NIPT of Trisomies 13 and 18Advanced Biomedical ResearchDecember 30, 2025Ali Najafi, Erfaneh Dastbaz, Artin Mehrabani, Morteza Rajabi, Ghazal Sedaghat Shayegan, Mohammad Miryounesi, Mohammad H. ModarressiThe enzymes used in this study included AciI, HhaI, HpaII, and HpyCH4IV (R0551L, R0139S, R0171S, and R0619S, respectively; New England BioLabs™). This analysis was performed on DNA extracted from CVS and PBL

(4) Macrophage/microglia-dependent mechanisms drive retinal pigment epithelium regeneration in zebrafishCell ReportsOctober 28, 2025Lyndsay L. Leach, Rebecca G. Gonzalez, Sayuri U. Jayawardena, Jeffrey M. GrossScientific,Cat#D128 "6-(octylamino)-2,4(1H,3H)-pyrimidinedione (6-OAU)",Cayman Chemical Company,Cat#17687 HpyCH4IV,New England Biolabs,Cat#R0619 SpeI-HF,New England Biolabs,Cat#R3133 MspI,New England Biolabs,Cat#R0106 SacII,New England Biolabs,Cat#R0157 "DNase

(5) MeCP2 binds to methylated DNA independently of phase separation and heterochromatin organisationNature CommunicationsMay 8, 2024Raphaël Pantier, Megan Brown, Sicheng Han, Katie Paton, Stephen Meek, Thomas Montavon, Nicholas Shukeir, Toni McHugh, David A. Kelly, Tino Hochepied, et al.(M. musculus and M. spretus) genomic DNA was digested with methylation-sensitive (HpyCH4IV, NEB cat. R0619L) or methylation-insensitive (ApoI, NEB cat. R3566L) restriction enzymes cutting within major satellite

(6) Non-small Cell Lung Cancer Epigenomes Exhibit Altered DNA Methylation in Smokers and Never-smokersGenomics, Proteomics & BioinformaticsSeptember 22, 2023Jennifer A. Karlow, Erica C. Pehrsson, Xiaoyun Xing, Mark Watson, Siddhartha Devarakonda, Ramaswamy Govindan, Ting Wangperformed with four enzymes: HpaII, SsiI, Hin6I, and HpyCH4IV (Catalog Nos. R0171S, R0551S, R0124S, and R0619S, New England Biolabs, Ipswich, MA). A base offset of 3 was used during sequencing. Adapter trimming,

(7) Generation and characterization of a novel gne Knockout Model in ZebrafishFrontiers in Cell and Developmental BiologyOctober 24, 2022Hagay Livne, Tom Avital, Shmuel Ruppo, Avi Harazi, Stella Mitrani-Rosenbaum, Alon DayaTypically, 2–5 µL of the PCR reaction were used for digestion with the HpyCH4IV restriction enzyme (#R0619L, New England Biolabs Inc.) or for other screening methods. Sexually mature F0 CRISPR injected fish were

(8) Sensitive and reproducible cell-free methylome quantification with synthetic spike-in controlsCell Reports MethodsSeptember 19, 2022Samantha L. Wilson, Shu Yi Shen, Lauren Harmon, Justin M. Burgener, Tim Triche, Scott V. Bratman, Daniel D. De Carvalho, Michael M. HoffmanPurification Kit,"Qiagen, Hilden, Germany",Cat# 28004 HpyCH4IV,"New England Biolabs, Ipswitch, MA, USA",Cat# R0619S HpaII,"New England Biolabs, Ipswitch, MA, USA",Cat# R171S AfeI,"New England Biolabs, Ipswitch, MA,

(9) A comprehensive approach for genome-wide efficiency profiling of DNA modifying enzymesCell Reports MethodsMarch 28, 2022Charalampos Kyriakopoulos et al.Streptavidin,ThermoFisher,11205D AMPureXP® beads,Beckman Coulter,A63880 R.AluI,NEB,R0137 R.HaeIII,NEB,R0108 R.HpyCH4V,NEB,R0619 Klenow exo-,NEB,M0212 T4 DNA Ligase,NEB,M0202 HotStarTaq DNA Polymerase,Qiagen,203207 ,, Critical

(10) The role of Niemann-Pick type C2 in zebrafish embryonic developmentDevelopmentMarch 15, 2021Wei-Chia Tseng, Ana J Johnson Escauriza, Chon-Hwa Tsai-Morris, Benjamin Feldman, Ryan K. Dale, Christopher A. Wassif, Forbes D. Porterand npc2y537 were digested with restriction enzyme BglII (R0144, New England BioLabs) and HpyCH4IV (R0619, New England BioLabs), respectively, at 37°C for 8 h. Restriction enzyme digestion products were resolved

(11) DNA Methylation Bisubstrate Inhibitors Are Fast-Acting Drugs Active against Artemisinin-Resistant Plasmodium falciparum ParasitesACS Central ScienceJanuary 22, 2020Flore Nardella et al.PBST 0.5 M NaCl and PBST, an unmethylated DNA specific restriction was induced by 5U of HpyCH4IV (NEB R0619L) for 1 h at 37 °C. After washing, the fluorescence was evaluated on an EnVision Multilabel reader (PerkinElmer).

(12) REC114 Partner ANKRD31 Controls Number, Timing, and Location of Meiotic DNA BreaksMolecular CellJune 1, 2019Michiel Boekhout et al.followed with digestion by AflII (NEB, R0520L) which recognizes em1 (+A) allele or with HrpCh4IV (NEB, R0619L), which recognizes em2 (+C) allele. The PCR product is 339 bp and restriction digestion products are

(13) Long-Term In Vitro Expansion of Epithelial Stem Cells Enabled by Pharmacological Inhibition of PAK1-ROCK-Myosin II and TGF-β SignalingCell reportsOctober 16, 2018Zhang C, Lee HJ, Shrivastava A, Wang R, McQuiston TJ, Challberg SS, Pollok BA, Wang T.

(14) Comprehensive Whole DNA Methylome Analysis by Integrating MeDIP-seq and MRE-seqMethods in Molecular BiologyJune 11, 2018Xiaoyun Xing, Bo Zhang, Daofeng Li, Ting Wangcat. no. R0171S).HinP1I (NEB; cat. no. R0124S).AciI (NEB; cat. no. R0551S).HpyCH4IV (NEB; cat. no. R0619S).Bsh1236I (Thermo Scientific; cat. no. ER0921). Gel electrophoresis system for size selection (Thermo

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HpyCH4IV

HpyCH4IV from New England Biolabs

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Sequence

New England Biolabs

Citations (14)

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HpyCH4IV from New England Biolabs

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