HpyCH4III from New England Biolabs

Product Specs
ItemHpyCH4III
CompanyNew England Biolabs
Price $75.00 Inquire
Catalog NumberR0618S
Quantity250 units
Concentration10 u/µL
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

ACN^GT

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) An in vitro transcription-based protocol for mapping HIV integration sites using lentiviral integration site sequencingSTAR ProtocolsMarch 1, 2026Kamil Więcek, Janusz Wiśniewski, Heng-Chang ChenAllPrep DNA/RNA Mini kit(50)],QIAGEN,Cat. No. 80204 RNase-free DNase I,New England Biolabs,M0303S HpyCH4III,New England Biolabs,R0618S BplI,Thermo Fisher Scientific,ER1311 MfeI-HF,New England Biolabs,R3589S Hpy166II,New England Biolabs,R0616S Agarose

(2) The influence of HIV sense and antisense transcripts on stochastic HIV transcription and reactivationComput Struct Biotechnol JAugust 6, 2025Kamil Więcek, Janusz Wiśniewski, Heng-Chang Chenfollowing the manufacturer’s instructions and digested using 2 µL 5000 U/mL HpyCH4III (New England Biolabs, R0618S) and 2 µL 10 U/µL BplI (Thermo Scientific, Cat. No. ER1011) in Tango buffer complemented with 1 × SAM

(3) Deletion of taf1 and taf5 in zebrafish capitulate cardiac and craniofacial abnormalities associated with TAFopathies through perturbations in metabolismBiology OpenJuly 15, 2023Jamison Leid, Ryan Gray, Peter Rakita, Andrew L. Koenig, Rohan Tripathy, James A. J. Fitzpatrick, Charles Kaufman, Lilianna Solnica-Krezel, Kory J. Lavine333-bp region including the corazoncito mutation, using the restriction enzyme HpyCh4III (NEB; cat. No. R0618) that bifurcates the amplicon into ∼161-bp doublets. All lines used in this study were raised and maintained

(4) Association study of polymorphisms in ABCA7, clusterin, and MS4A6A genes with Alzheimer’s disease in the Egyptian populationTurkish Journal of BiochemistryDecember 23, 2020Hanem G. Abd Elrahman, Abeer Ramadan, Eman M. Saleh, Alia A. Saleh, Noha A. Sabry, Mohamed R. Mohamedvariant) was 642 bp; it was digested by HpyCH4III restriction enzyme (New England, BioLabs, catalog no #R0618S). The ampliﬁed PCR product for Clusterin rs11136000 (intronic variant) SNP was 389 bp; it was digested

(5) Real-time kinetics and high-resolution melt curves in single-molecule digital LAMP to differentiate and study specific and non-specific amplificationNucleic Acids ResearchApril 17, 2020Justin C Rolando, Erik Jue, Jacob T Barlow, Rustem F IsmagilovCAC8I (#R0579S), Hpy166II (#R0616S), ACCI (#R0161S), AciI (#SR0551S), MseI (#R0525S) and HpyCH4III (#R0618S) purchased from New England Biolabs and were conducted in 50 μl reaction volumes containing 1 μl enzyme,

(6) The homozygous CX3CR1-M280 mutation impairs human monocyte survivalJCI insightFebruary 8, 2018Collar AL, Swamydas M, O'Hayre M, Sajib MS, Hoffman KW, Singh SP, Mourad A, Johnson MD, Ferre EM, Farber JM, et al.by incubating the resulting PCR reaction product with 1.5 U of Bst4CI (New England BioLabs, catalog R0618S) in a 20-μl reaction as per the manufacturer’s instructions. This resulted in 107- and 204-bp fragments

(7) Natural variation in a single amino acid substitution underlies physiological responses to topoisomerase II poisonsPLOS GeneticsJuly 12, 2017Stefan Zdraljevic, Christine Strand, Hannah S. Seidel, Daniel E. Cook, John G. Doench, Erik C. Andersenusing oECA1087 and oECA1124. The PCR products were digested using the HpyCH4III restriction enzyme (R0618L, New England Biolabs, Ipswich, MA). Differential band patterns signified successfully edited strains

(8) Position effects influence HIV latency reversalNature Structural & Molecular BiologyJanuary 1, 2017Heng-Chang Chen, Javier P Martinez, Eduard Zorita, Andreas Meyerhans, Guillaume J Filiondigested by 2 µL 10 U/µL BplI (Thermo Fisher Scientific, ER1311) and 2 µL 5,000 U/mL HpyCH4III (NEB, R0618S) in buffer Tango complemented with 1X SAM in 50 µL final volume at 37 ℃ for 3 hours. The reaction was stopped

(9) Detecting ultralow-frequency mutations by Duplex SequencingNature ProtocolsNovember 1, 2014Scott R Kennedy et al.AM9937)T4 polynucleotide kinase (NEB, cat. no. M0201; includes reaction buffer)HpyCH4III (NEB, cat. no. R0618; includes CutSmart buffer)dNTPs, 2.5 mM each (Promega, cat. no. U1511)Ethanol (100%, 200 proof; Sigma-Aldrich,

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HpyCH4III

HpyCH4III from New England Biolabs

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HpyCH4III from New England Biolabs

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