HpaII from New England Biolabs

Product Specs
ItemHpaII
CompanyNew England Biolabs
Price $72.00 Inquire
Catalog NumberR0171S
Concentration10-50 u/µL
Quantity2000 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

C/CGG

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) A Multi-Locus and Machine Learning-Based Assessment of SNCA Variants in Alzheimer's DiseaseInt J Mol SciJune 5, 2026Hatice Segmen, Mustafa Yildizrestriction enzyme HpaII (recognition site: C↓CGG; New England Biolabs, Ipswich, MA, USA; Cat. No. R0171). The A allele remained uncut, yielding a single 316 bp fragment, while the G allele was digested into

(2) Mutual potentiation of IPA1 and OsNPR1 enhances rice immunityPlant CellMay 20, 2026Zhen Wang, Mingming Liu, Rujuan Yan, Xiaohan Zhang, Xiaoguang Song, Guosheng Xiong, Jiayang Li, Zuhua He, Dong-Lei YangData Set S5). The IPA1 DNA fragment was cloned into the pCAMBIA1305-3xFLAG vector digested with HpaII (R0171S, NEB) and HindIII (R3104S, NEB) through homologous recombination using a ClonExpress II One Step Cloning kit (C112

(3) Pluripotent stem cell-based screening uncovers sildenafil as a treatment for mitochondrial diseaseCellMarch 19, 2026Annika Zink, Dao-Fu Dai, Annika Wittich, Marie-Thérèse Henke, Giulia Pedrotti, Sonja Heiduschka, Guillem Santamaria, Tancredi Massimo Pentimalli, Christian Brueser, Sofia Notopoulou, et al.Dye®,Invitrogen™,C1430 MitoSpy™ Orange,BioLegend,424803 Sildenafil-d3,CDN isotopes,D-6366 HpaII,NEB,R0171 StuI,NEB,R0187 XbaI,NEB,R0145 FCCP,Biozol,SEL-S8276 Antimycin A,Sigma-Aldrich,A8674 Oligomycin,Sigma-Aldrich,O4876-5MG CCCP,Sigma-Aldrich,555602 Propidium iodide

(4) An image-based transcriptomics atlas reveals the regional and microbiota-dependent molecular, cellular, and spatial structure of the murine gutCell Host MicrobeFebruary 19, 2026Rosalind J. Xu, Hao Zhang, Paolo Cadinu, Phillip B. Nicol, Uli S. Herrmann, Tyrone Lee, Ludwig Geistlinger, Rafael A. Irizarry, Jeffrey R. Moffitt,ThermoFisher Scientific,Cat#R0151 100 mM dGTP,ThermoFisher Scientific,Cat#R0161 100 mM dTTP,ThermoFisher Scientific,Cat#R0171 Murine RNase inhibitor,NEB,Cat#M0314L RNasin ribonuclease inhibitor,VWR,Cat#N2615 Proteinase K,NEB,Cat#P8107S "Phosphate

(5) DNA methylation and lncRNA control asynchronous DNA replication at specific imprinted gene domainsNature CommunicationsJanuary 21, 2026Yui Imaizumi, François Charon, Caroline Surcis, Christel Picard, Pol Arnau-Romero, Jean-Christophe Andrau, Daan Noordermeer, Benoit Moindrot, Jean-Charles Cadoret, Robert Feilwas split into two Eppendorf tubes subsequently. To one tube, 1 μl of HpaII (New England Biolabs, #R0171) was added, but not to the other. Following 37 °C overnight incubation, DNAs were extracted and enzymes

(6) Bacterial chromatin remodeling associated with transcription-induced domains at pathogenicity IslandsNature CommunicationsJanuary 8, 2026Mounia Kortebi, Mickaël Bourge, Romain Le Bars, Erwin Van Dijk, Charles J. Dorman, Stéphanie Bury-Moné, Frédéric Boccard, Virginia S. LioyAfter removing 80 μL of non-digested control, 200 units of HpaII enzyme (New England Biolabs, Cat# R0171L) were added to the digestion mix. Both tubes were then incubated for 3 h at 37 °C with agitation. Following

(7) Identifying Differentially Methylated Sites for Methylation-Sensitive qPCR-based NIPT of Trisomies 13 and 18Advanced Biomedical ResearchDecember 30, 2025Ali Najafi, Erfaneh Dastbaz, Artin Mehrabani, Morteza Rajabi, Ghazal Sedaghat Shayegan, Mohammad Miryounesi, Mohammad H. Modarressirestriction enzymes. The enzymes used in this study included AciI, HhaI, HpaII, and HpyCH4IV (R0551L, R0139S, R0171S, and R0619S, respectively; New England BioLabs™). This analysis was performed on DNA extracted from

(8) Rat models of lactose intolerance: sexually dimorphic comorbidities with neuropsychiatric disorders modulated by tryptophan hydroxylase and the dopaminergic systemScientific ReportsNovember 26, 2025Marcella C. Galvão, Nathalia A. Galvão, Vanessa S. A. da Silva, Tifany V. Rocha, Daniel N. L. Santos, Liebert B. Carvalho, Rodrigo A. F. da Silva, Thiago Berti Kirstentreated with endonucleases purchased from New England Biolabs, T4-Bgt (M0357S), MspI (R0106T) and HpaII (R0171L)76. Primer sequences were designed in regulatory regions with CpG islands within DnaseI hypersensitive

(9) A ternary-code DNA methylome atlas of mouse tissuesGenome BiolOctober 7, 2025Sol Moe Lee, David C. Goldberg, Cameron Cloud, Jared B. Parker, Christopher Krapp, Christian E. Loo, Elliot Kim, Ivan Zhao, Chengcheng Jin, Rishi Porecha, et al.lambda DNA: A mixture was prepared by combining 20 ng of methylated lambda DNA with 0.2 µl of HpaII (NEB, R0171S), 10X buffer, and autoclaved ultrapure water, resulting in a total volume of 10 µl. The mixture was

(10) Carrot (Daucus carota L.) as Host for Pentastiridius leporinus and Phloem-Restricted Pathogens in GermanyBiology (Basel)September 1, 2025Natasha Witczak, Salma Benaouda, Friederike Wahl, Hendrik Göbbels, Christian Lang, Barbara Jarausch, Michael MaixnerAmplicons of nested PCR of the tuf-gene were subjected to digestion using HpaΙΙ restriction enzyme (R0171S, New England Biolabs, Ipswich, UK) [30]. The Stolbur-specific stamp gene was amplified by nested PCR

(11) An image-based transcriptomics atlas reveals the regional and microbiota-dependent molecular, cellular, and spatial structure of the murine gutbioRxivJuly 30, 2025Rosalind J. Xu, Paolo Cadinu, Phillip B. Nicol, Uli S. Herrmann, Tyrone Lee, Ludwig Geistlinger, Rafael A. Irizarry, Jeffrey R. Moffitt,ThermoFisher Scientific,Cat#R0151 100 mM dGTP,ThermoFisher Scientific,Cat#R0161 100 mM dTTP,ThermoFisher Scientific,Cat#R0171 Murine RNase inhibitor,NEB,Cat#M0314L RNasin ribonuclease inhibitor,VWR,Cat#N2615 Proteinase K,NEB,Cat#P8107S "Phosphate

(12) CpG island demethylation and recruitment of SP1 to the promoter region regulates human thymic stromal lymphopoietin expressionEpigeneticsJuly 14, 2025Krishna Priya Ganti, Milan SurjitHaCaT cells using Qiagen DNeasy kit following manufacturer’s instructions. Isolated DNA was subject to HpaII (R0171S, NEB, MA, USA) and MspI (R0106S, NEB, MA, USA) restriction digestion, followed by PCR amplification

(13) Seizure-related death exhibits a circadian rhythm independent of seizure timing or sleep in a mouse model of Dravet syndromeJ PhysiolJune 2, 2025Benjamin L. Kreitlow, Ana T. Novella-Maciel, Ariana M. Hernández Vázquez, Gordon F. Buchananrestriction digest site. Following PCR‐based amplification, samples were restriction digested (Catalog #R0171; New England Biolabs, Ipswich, MA, USA) and then run on a 2% agarose gel. Wild‐type animals had two

(14) Seizure‐related death exhibits a circadian rhythm independent of seizure timing or sleep in a mouse model of Dravet syndromeJ PhysiolJune 2, 2025Benjamin L. Kreitlow, Ana T. Novella‐Maciel, Ariana M. Hernández Vázquez, Gordon F. Buchananrestriction digest site. Following PCR‐based amplification, samples were restriction digested (Catalog #R0171; New England Biolabs, Ipswich, MA, USA) and then run on a 2% agarose gel. Wild‐type animals had two

(15) From Rare to Common: Genetic Insights into TLR7 Variants in a Multicentric Spanish Study on COVID-19 SeverityJ Clin ImmunolMay 27, 2025Arnau Antolí, Gardenia Vargas-Parra, Angels Sierra-Fortuny, Jose Luis Gomez-Vazquez, Paula Rofes, Elisabet Munté, Julen Viana-Errasti, Raúl Marín-Montes, Adriana López-Doriga, Lidia Feliubadaló, et al.X-inactivation was assessed via the HUMARA assay [54]. DNA samples were either digested with HpaII (R0171S, New England Biolabs, Ipswich, MA, USA) or incubated without the enzyme. The androgen receptor locus

(16) Clinical response to azacitidine in MDS is associated with distinct DNA methylation changes in HSPCsNat CommunMay 13, 2025Julie A. I. Thoms, Feng Yan, Henry R. Hampton, Sarah Davidson, Swapna Joshi, Jesslyn Saw, Chowdhury H. Sarowar, Xin Ying Lim, Andrea C. Nunez, Purvi M. Kakadia, et al.reverse F-oligos plus 40 nM each of forward and reverse outer primers and FAM probe), 2U HpaII (NEB #R0171L), 1U DraI (NEB #R0129L), 0.5U Hot Start Taq DNA Polymerase (NEB #M0495L)) were cycled at 37 °C for 15

(17) MSH2 is not required for either maintenance of DNA methylation or repeat contraction at the FMR1 locus in fragile X syndrome or the FXN locus in Friedreich's ataxiaEpigenetics ChromatinApril 28, 2025Jessalyn Grant-Bier, Kathryn Ruppert, Bruce Hayward, Karen Usdin, Daman Kumarieither only HindIII-HF (New England Biolabs, R3104S) or HindIII-HF and HpaII (New England Biolabs, R0171S). PCR was performed using 5 µL digested DNA (100 ng) in a 50 µL reaction containing 0.8 µM each of Not_FraxC

(18) Foxm1 promotes differentiation of neural progenitors in the zebrafish inner earDevelopmental BiologyApril 1, 2025Maria Ali, James W. Kutlowski, Jorden N. Holland, Bruce B. Rileyreverse primer 5′-CTTTCCCAAGCTCAGACCC-3′, and the resulting amplicon was digested with HpaII (NEB: R0171S). Digestion of wild-type DNA produces ~180 bp fragments, while the mutant amplicon remains uncut due

(19) Deep characterization of females with heterozygous Duchenne muscular dystrophy mutationsJ NeurolMarch 4, 2025Pietro Riguzzi, Daniele Sabbatini, Aurora Fusto, Sara Vianello, Beatrice Merlo, Vittoria Zangaro, Giuliana Capece, Domenico Gorgoglione, Gianni Sorarù, Riccardo Bariani, et al.[19], we were able to identify the more active X chromosome. To do so, the DNA was digested with HpaII (R0171S, NEB) and HhAI (R0139S, NEB) methylation sensitive enzymes and purified by phenol/chloroform and ether

(20) Bisulfite‐free PCDHGB7 methylation in urine enables early noninvasive detection of urothelial carcinomaBioengineering & Translational MedicineFebruary 26, 2025Zhicong Yang, Qing Chen, Shihua Dong, Peng Xu, Zhanrui Mao, Yaping Dong, Wei Li, Wenxuan Li, Yang Han, Lihe Dai, et al.urine was quantified by MSRE‐qPCR using 100 ng gDNA digested with HhaI (NEB, R0139) and HpaII (NEB, R0171) endonuclease. GAPDH gene, absent of any MSRE cutting sites, was designed for normalization (GAPDH N).

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HpaII

HpaII from New England Biolabs

Sequence

New England Biolabs

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HpaII from New England Biolabs

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