HpaI from New England Biolabs

Product Specs
ItemHpaI
CompanyNew England Biolabs
Price $70.00 Inquire
Catalog NumberR0105S
Concentration5 u/µL
Quantity500 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

GTT/AAC

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Tumor metabolic adaptation induced by L-asparaginase reveals a vulnerability to PARP1/2 inhibitor in B-cell lymphomasNature CommunicationsFebruary 27, 2026Anaïs Aussel, Ivan Nemazanyy, Ashaina Vandenberghe, Pauline Meola, Noémie Dorat, Rachel Paul, Lama Habbouche, Laurie Exposito, Nicolas Nottet, Virginie Petrilli, et al.5′-GGTTAACTCACGTAGAATCGAGACCGAGGAGAGGGTTAGGGATAGGCTTACCGAAGCAGAACTGGAAAGCCTCCAATAC-3′. Then, the amplicon was digested using SalI (R3136S, New England Biolabs) and HpaI (R0105S, New England Biolabs) restriction enzymes. The pMIG vector was linearized using XhoI (R0146S, New England Biolabs

(2) PRISM-Seq: An Ultra-sensitive Sequencing Approach For Mapping Lentiviral Integration SitesbioRxivFebruary 7, 2026V K Pal, M Canis, E Stone, N Board, K Lenart, M Fumagalli, C Kovacs, R B Jones, M Nussenzweig, T Hatziioannou, et al.sample. Purified MDA products were digested using three blunt-end restriction enzymes - HpaI (NEB #R0105L), SspI (NEB #R3132L), and DraI (NEB #R0129L) - to fragment genomic DNA and facilitate subsequent ligation-based

(3) A Rapid and Cost-Effective Pipeline to Identify and Capture BGCs From Bacterial Draft GenomesBIO-PROTOCOLDecember 20, 2025Marco A. Campos-Magaña, Vitor A. P. Martins Dos Santos, Luis Garcia-MoralesGenomic DNA Plug kits (Bio-Rad, catalog number: 170-3592) 37. HpaI (New England Biolabs, catalog number: R0105S) 38. BamHI-HF (New England Biolabs, catalog number: R3136S) 39. NaCl (Sigma-Aldrich, catalog number:

(4) Comprehensive benchmarking of genome editing quantification methods for plant applicationsiScienceApril 29, 2025Zheng Gong, Yan Zhang, Di Xia, Sohye Yoon, Peter Alexander Crisp, José Ramón Botella,New England Biolabs,R3589S RsaI,New England Biolabs,R0167S BsrDI,New England Biolabs,R0574S HpaI,New England Biolabs,R0105S BstNI,New England Biolabs,R0168S NuSieve GTG™ agarose,Lonza Bioscience,50084 ,, Critical commercial

(5) In Vitro Analysis of AKR1D1 Interactions with Clopidogrel: Effects on Enzyme Activity and Gene ExpressionBalkan J Med GenetMarch 12, 2025K Shutevska, Panovska T Kadifkova, Z Zhivikj, Nestorovska A Kapedanovskaassays.–Expression Assay ReagentsHpaI Restriction Enzyme (New England Biolabs, Ipswich, MA, USA; Cat# R0105S): Used for linearizing the AKR1D1 plasmid to generate a calibration curve for qRT-PCR quantification.Shrimp

(6) CRISPR-Enabled Autonomous Transposable Element (CREATE) for RNA-based gene editing and deliveryEMBO ReportsJanuary 9, 2025Yuxiao Wang, Ruei-Zeng Lin, Meghan Harris, Bianca Lavayen, Neha Diwanji, Bruce McCreedy, Robert Hofmeister, Daniel Gettsbly mix,New England BioLabs,E2621L QIAprep Spin Miniprep Kit,Qiagen,27106 HpaI,New England BioLabs,R0105L HiScribe T7 mRNA Kit with CleanCap Reagent AG,New England BioLabs,E2080S GeneJET PCR Purification Kit

(7) Fluorescence‐based CRISPR interference system for controlled genetic repression and live single‐cell imaging in mycobacteriaFEBS LettersDecember 1, 2024Janïs Laudouze, Vanessa Point, Wafaa Achache, Céline Crauste, Stéphane Canaan, Pierre SantuccipJL30 templates. DNA fragments were purified and ligated into HpaI‐digested pIRL117 and pIRL2 (NEB; #R0105L) to generate the pJL31/pJL32 and pJL33/pJL34 vectors, respectively. All vectors generated were fully

(8) K103N, V106M and Y188L Significantly Reduce HIV-1 Subtype C Phenotypic Susceptibility to DoravirineVirusesSeptember 20, 2024Nikita Reddy, Maria Papathanasopoulos, Kim Steegen, Adriaan Erasmus Basson(New England Biolabs (NEB), Ipswich, MA, USA, Cat No. R3150S) and HpaI (NEB, Ipswich, MA, USA, Cat. No. R0105S). The digested vectors were dephosphorylated using Shrimp Alkaline Phosphatase (NEB, Ipswich, MA, USA,

(9) Long non-coding RNAs involved in Drosophila development and regenerationNAR Genomics and BioinformaticsAugust 16, 2024Carlos Camilleri-Robles, Raziel Amador, Marcel Tiebe, Aurelio A Teleman, Florenci Serras, Roderic Guigó, Montserrat CorominasBiotek,D7043-01 Fast Start Universal SYBR Green Master (Rox),Roche,4913850001 HpaI,New England Biolabs,R0105S KpnI,Promega,R6341 M-MLV Reverse Transcriptase,Invitrogen,10338842 MinElute PCR purification kit,Qiagen,28004 MinElute

(10) Protocol for scarless genome editing of human pluripotent stem cell based on orthogonal selective reportersSTAR ProtocolsJune 21, 2024Yun Zhao, Zihang Pan, Zixuan Hong, Mengze Sun, Yi Hong, Xiaohong Peng, Xiaoxia Li, Xi Wang, Kai Wang,New England Biolabs,Cat#R3195 HindIII-HF,New England Biolabs,Cat#R3104 XbaI,New England Biolabs,Cat#R0145 HpaI,New England Biolabs,Cat#R0105 T4 DNA ligase,New England Biolabs,Cat#M0202 EDTA,Thermo Fisher Scientific,Cat15575020 Bovine serum albumin

(11) The Atad5 RFC-like complex is the major unloader of proliferating cell nuclear antigen in Xenopus egg extractsJournal of Biological ChemistryJanuary 1, 2024Yoshitaka Kawasoe, Sakiko Shimokawa, Peter J. Gillespie, J. Julian Blow, Toshiki Tsurimoto, Tatsuro S. TakahashiSupporting Table S1. The amplified fragment was digested with BamHI and HpaI (New England Biolabs, #R0105) and inserted between the BamHI and HpaI sites in pCSII-EF-mAG-hATAD5-FLAG, resulting in pCSII-EF-mAG-hATAD5-K1138E-FLAG. Protein expression

(12) Understanding cancer drug resistance with Sleeping Beauty functional genomic screens: Application to MAPK inhibition in cutaneous melanomaiScienceOctober 20, 2023Eliot Y. Zhu, Jacob L. Schillo, Sarina D. Murray, Jesse D. Riordan, Adam J. DupuyTaq,Thermofisher,Catalog #: 10966018 LMPCR reagent: ATP,Thermofisher,Catalog #: R0441 Enzyme: HpaI,NEB,Catalog #: R0105S Enzyme: EcoNI,NEB,Catalog #: R0521S Enzyme: BamH1,NEB,Catalog #: R3136S Enzyme: dsDNA Fragmentase,NEB,Catalog

(13) Mutational screens highlight glycosylation as a modulator of colony-stimulating factor 3 receptor (CSF3R) activityJournal of Biological ChemistryJune 1, 2023Michael J. Hollander, Stacy A. Malaker, Nicholas M. Riley, Idalia Perez, Nayla M. Abney, Melissa A. Gray, Julia E. Maxson, Jennifer R. Cochran, Carolyn R. BertozziScientific NC1240162)/BsaBI (Thermo Fisher Scientific 50-812-207) (IL-31Rα), HpaI (New England Biolabs R0105S)/AgeI-HF (LEPR), or BamHI-HF/PsiI-v2 (IL-2Rγ), and the backbones were purified by gel extraction. The

(14) IGF-1 Therapy Improves Muscle Size and Function in Experimental Peripheral Arterial DiseaseJACC: Basic to Translational ScienceJune 1, 2023Gengfu Dong, Chatick Moparthy, Trace Thome, Kyoungrae Kim, Feng Yue, Terence E. RyanThe scAAV-CMV-GFP plasmid was digested with EcoRI and HpaI (New England Biolabs, cat. nos. R3101 and R0105) and the linearized scAAV-CMV plasmid without the GFP was gel purified. The rat IGF1 coding sequence

(15) Restraint of IFN-γ expression through a distal silencer CNS-28 for tissue homeostasisImmunityMay 9, 2023Cui K, Chen Z, Cao Y, Liu S, Ren G, Hu G, Fang D, Wei D, Liu C, Zhu J, et al.,New England Biolabs,Cat#M0203L rCutSmart buffer,New England Biolabs,Cat# B6004S Hpa I,New England Biolabs,Cat# R0105L MluCI,New England Biolabs,Cat# R0538L NlaIII,New England Biolabs,Cat# R0125L Proteinase K,Roche,Cat#

(16) Next-generation plasmids for transgenesis in zebrafish and beyondDevelopmentApril 15, 2023Cassie L. Kemmler, Hannah R. Moran, Brooke F. Murray, Aaron Scoresby, John R. Klem, Rachel L. Eckert, Elizabeth Lepovsky, Sylvain Bertho, Susan Nieuwenhuize, Sibylle Burger, et al.vector pDestTol2A2 (Tol2kit 394) (Kwan et al., 2007) was digested with BglII (NEB R0144S) and HpaI (NEB R0105S) restriction enzymes. The digested plasmid was purified using the Wizard SV Gel and PCR Clean-Up System

(17) The TRESLIN-MTBP complex couples completion of DNA replication with S/G2 transitionMolecular cellSeptember 15, 2022Zonderland G, Vanzo R, Gadi SA, Martín-Doncel E, Coscia F, Mund A, Lerdrup M, Benada J, Boos D, Toledo L.Kit,Agilent,Cat# 200523 "BamHI, EcoRI, HpaI, rCutsmart",New England Biolabs,"Cat# R0136S, R3101S, R0105S, B6004S"

(18) Biological features of TcM: A new Trypanosoma cruzi isolate from Argentina classified into TcV lineage✰Current Research in Microbial SciencesAugust 1, 2022Santiago José Martinez, Gonzalo Nicolás Nardella, Matías Exequiel Rodríguez, Cynthia Vanesa Rivero, Fernán Agüero, Patricia Silvia Romano20 µl of TcSC5D amplification products (832 bp) were simultaneously digested with 1 U of HpaI (NEB R0105) and 1 U of SphI (NEB R0182) for 1 h at 37 °C. Restriction fragments were then resolved in 2% TBE-agarose

(19) Tumoral microenvironment prevents de novo asparagine biosynthesis in B cell lymphoma, regardless of ASNS expressionScience advancesJuly 8, 2022Grima-Reyes M, Vandenberghe A, Nemazanyy I, Meola P, Paul R, Reverso-Meinietti J, Martinez-Turtos A, Nottet N, Chan WK, Lorenzi PL, et al.[DNA Polymerase I, Large (Klenow) Fragment, M0210S, New England Biolabs], and the mAsns-v5 sequence was cloned into the Hpa I (R0105S, New England Biolabs) restriction site of the retroviral GFP-encoded pMIG vector (9044, Addgene) previously

(20) U1A is a positive regulator of the expression of heterologous and cellular genes involved in cell proliferation and migrationMolecular therapy. Nucleic acidsJune 14, 2022Rovira E, Moreno B, Razquin N, Hjerpe R, Gonzalez-Lopez M, Barrio R, Ruiz de Los Mozos I, Ule J, Pastor F, Blazquez L, et al.described.10 The RL sequences were extracted from these plasmids by digestion with NheI (R3131; NEB) and HpaI (R0105; NEB) restriction enzymes and were then cloned into the EcoRV and HpaI sites of pAC5.1 (Invitrogen).

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HpaI

HpaI from New England Biolabs

Sequence

New England Biolabs

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HpaI from New England Biolabs

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