HindIII-HF from New England Biolabs

Product Specs
ItemHindIII-HF
CompanyNew England Biolabs
Price $64.00 Inquire
Catalog NumberR3104S
Quantity10000 units
Concentration20,000 units/ml
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

AAGCTT

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) MFF budding from mitochondria regulates melanosome size and maturationNature CommunicationsMarch 14, 2026Ana Paula Magalhães Rebelo, Aurora Maracani, Samuele Greco, Federica Dal Bello, Lucia Santorelli, Marco Gerdol, Alberto Pallavicini, Tomas Knedlik, Sara Schiavon, Luca Scorrano, et al.pEGFP-C1 (Addgene, Cat. No. 6084-1) were digested with HindIII-HF (New England Biolabs NEB Cat. No. R3104S) and BamHI-HF (New England Biolabs NEB Cat. No. R3136S) restriction enzymes, run in a 2% agarose gel

(2) Armored macrophage-targeted CAR-T cells reset and reprogram the tumor microenvironment and control metastatic cancer growthCancer CellMarch 9, 2026Jaime Mateus-Tique, Ashwitha Lakshmi, Bhavya Singh, Rhea Iyer, Alfonso R. Sánchez-Paulete, Chiara Falcomatà, Matthew Lin, Gvantsa Pantsulaia, Alexander Tepper, Trung Nguyen, et al.between the CD3z domain and the T2A-GFP sequence using using EcorI-HF (NEB # R3101S) and HindIII-HF (NEB R3104S) for their respective restriction sites. For the CMV driven IL-12 vector, we used pMSCVsin-PGK-GFP plasmid,

(3) Protocol for targeted gene manipulation and thermogenic evaluation in mouse brown adipocytesSTAR ProtocolsMarch 1, 2026Jakub Bunk, Drishti Soni, Matthias Calderon, Bozena Samborska, Lawrence KazakPfuTurbo DNA Polymerase,Agilent,600250 Deoxynucleotide (dNTP) Solution Mix,New England Biolabs (NEB),N0447S HindIII-HF,New England Biolabs (NEB),R3104S SalI-HF,New England Biolabs (NEB),R3138S 10× rCutSmart Buffer,New England Biolabs (NEB),B6004S "Nuclease-free

(4) Generation and characterization of human induced pluripotent stem cells from neuropathologically confirmed multiple system atrophy patient-derived fibroblastsFrontiers in ImmunologyFebruary 23, 2026Mireia Alemany-Ribes, Alexandra Pérez-Soriano, Almudena Santos, Jacqueline Severino, Adrià Dangla-Valls, Martín Gigirey-Suárez, Dory Cohen, Mario Ezquerra, Ruben Fernandez-Santiago, Manel Fernandez, et al."Insulin solution, human",Sigma-Aldrich,Cat#I9278 HEPES,Sigma-Aldrich,Cat#H4034 Restriction enzyme HindIII high fidelity,New England Biolabs,Cat#R3104S Colcemid™ solution in PBS,Gibco-ThermoFisher Scientific,Cat#15212012 "Paraformaldehyde, 4% (w/v) in

(5) Ring finger protein 213 regulates B-cell receptor signaling, metabolism, and development in B lymphocytesSignal Transduction and Targeted TherapyFebruary 18, 2026Ziyin Zhang, Nanshu Xiang, Qian Liu, Yanfeng Li, Linhua Wang, Xinpu Yang, Ju Liu, Lichen Zhang, Liaoxun Lu, Yinming Liang, et al.amplified DNA fragments were subsequently cloned and inserted into the pcDNA3.1 vector by HindIII (NEB, R3104S) and XbaI (NEB, R0145S) dual digestion, followed by T4 ligation (Takara, 2011B). The plasmids expressing

(6) ERVWE1 Impairs Mitochondrial Homeostasis and Promotes Neuronal Apoptosis via the miR-27b-3p/BNIP3 Axis in SchizophreniaVirusesFebruary 14, 2026Yaru Su, Kexin Zhao, Mengqi Zhang, Jiahang Zhang, Zhao Lv, Fangyi Hou, Xu Zhang, Zhao Zhang, Fan ZhushRNA expression vector using BamHI-HF (R3136S; New England Biolabs, Ipswich, MA, USA) and HindIII (R3104S; New England Biolabs, Ipswich, MA, USA) restriction enzymes. MiR-27b-3p mimic and corresponding negative

(7) Evolution of Translation Initiation Factor 2 Extensions Links Initiation to Bacterial Stress ResponsebioRxivFebruary 11, 2026Evrim Fer, Rajdeep Banerjee, Katsumi Hagino, Kaitlyn M. McGrath, Betül Kaçarlength E. coli infB gene and ΔN IF2 (Δ1–294) genes were cloned into pET21a(+) using HindIII-HF (NEB-R3104S) and EcoRI (NEB-R3101S) restriction enzymes. For in vivo experiments, pTrcHisB were used as backbone.

(8) A prophage-expressed type IV pilus component provides anti-phage defenseCell ReportsJanuary 27, 2026Kristina M. Sztanko, Taylor J. Ellison, Jill Greenlaw, Tatiana Lenskaia, P. Nathael Javorčík, Alexa D. Fitzpatrick, Karen L. Maxwell, Courtney K. Ellison, Alan R. Davidson,New England Biolabs,Cat# R3193S EcoRI-HF,New England Biolabs,Cat# R3101S HindIII-HF,New England Biolabs,Cat# R3104S KpnI-HF,New England Biolabs,Cat# R3142S XbaI-HF,New England Biolabs,Cat# R0145S BsaI-HF v2,New England Biolabs

(9) Construction and Biological Characterization of ORF133-Deletion Mutant of Lumpy Skin Disease VirusVirusesJanuary 24, 2026Qunhua Ke, Miaomiao Li, Yao Peng, Xiangwei Wang, Yuefeng Sun, Xiangping Yin, Yanming WeiTransfection Reagent,C0533,Beyotime,Shanghai/China Beyotime,Shanghai/China,RIPA Lysis Buffer,P0013C HindIII-HF®,R3104S,Neb,Ipswich/MA/USA Neb,Ipswich/MA/USA,EcoRI-HF®,R3101T Neb,Ipswich/MA/USA,KpnI-HF®,R3142S Neb,Ipswich/MA/USA,XbaI,R0145S Neb,Ipswich/MA/USA,T4 DNA Ligase

(10) Loss of ATM causes R-loop-associated transcriptional dysregulation and attenuates the related response to DNA damageJournal of Biological ChemistryJanuary 14, 2026Katherine R. Westover, Yingzi Hou, Feng Wang, Yilin Wang, Yangping Li, Rachel Seong, Jie Xu, Zhexing Wen, Bing Yaorestriction enzymes (BsrGI, EcoRI, HindIII, SspI, and XbaI, 30 units each) (NEB, #3575S, #R3101S, #R3104S, #R3132L, and #R0145S) at 37 °C overnight. The digested nucleic acids were then recovered by phenol–chloroform

(11) Late Stage Mannan Metabolism in Cellvibrio japonicus Requires the Combined Action of a Mannosyl‐Glucose Phosphorylase and a Mannobiose EpimeraseMolecular MicrobiologyJanuary 12, 2026Jessica K. Novak, Jeffrey G. Gardnerkit (Qiagen, cat. no. 27106) and then digested with EcoRI‐HF & HinDIII‐HF (NEB, cat. no. R3101S and R3104S). Primer design used Snapgene (GSL Biotech LLC). Plasmid construction was isothermally assembled using

(12) The gain-of-function TREM2-T96K mutation increases risk for Alzheimer’s disease by impairing microglial functionNeuronJanuary 7, 2026Dominika J. Pilat, Hoang Le, Dmitry Prokopenko, Chih-Chung Jerry Jerry Lin, William A. Eimer, Luisa Quinti, Evan P. Gavrilles, Sheyla N. Garcia, Sara N. Heitman, Alzheimer’s Disease Neuroimaging Initiative (ADNI), et al.pairs (bp) were treated for 3 hours with the restriction enzyme HindIII (New England BioLabs (NEB), #R3104S), which digested only the mutant PCR product into 216- and 286-bp fragments. Brain sample preparation Eight-month-old

(13) FoxP3 forms a head-to-head dimer in vivo and stabilizes its multimerization on adjacent microsatellitesCell ReportsDecember 23, 2025Fangwei Leng, Ryan Clark, Wenxiang Zhang, Thibault Viennet, Cuidie Wang, Haribabu Arthanari, Xi Wang, Sun Hur,New England Biolabs,Cat# R3136 XmaI,New England Biolabs,Cat# R0180S HindIII-HF,New England Biolabs,Cat# R3104S Deoxyribonuclease,Sigma,Cat# DN25 "Bis-Tris native PAGE, 3-12%",Invitrogen,Cat# BN1003BOX "Novex

(14) Identification of intestinal enteroendocrine cell subtypes and their associated hormones in zebrafishPLoS BiolDecember 18, 2025Margaret Morash, Richard G. Kay, Erik J. Soderblom, Grace H. MacLean, Jia Wen, Peyton J. Moore, Colin R. Lickwar, Mujahid Ali Shah, Julia Ganz, Fiona M. Gribble, et al.cttccgcggCACCTTAGTCTTAATTCTTTGCTACATAC. It was then cloned into the p5E-MCS [103] via digestion of insert and backbone with HindIII (NEB, R3104S) and SacII (NEB, R01575S) followed by phosphatase treatment (NEB, M0289S) and ligation (NEB, M0202S).

(15) USPPAR is a cost-effective, scalable, and highly sensitive single-cell RNA sequencing workflow compatible with diverse specimensPLoS BiolDecember 15, 2025Ya-Wen Hsueh, Po-Min ChiangP7070L); PEG8000 (Sigma, P2139); Sepharose CL-6B (GE Healthcare, CAS 62610-50-8); HindIII-HF (NEB, R3104S); ThermoPol buffer (NEB, B9004S); carboxylate paramagnetic particles (Cytiva, 65152105050250); Halt

(16) Sequestration of the phagocyte metabolite itaconate by Pseudomonas aeruginosa RpoN promotes successful pulmonary infectionNature CommunicationsDecember 13, 2025Ayesha Z. Beg, Zihua Liu, Ying-Tsun Chen, Absar Talat, Griffin Gowdy, Jake Miller, Lindsey Florek, Lars Dietrich, Chu Wang, Ian Lewis, et al.reagents Dream Taq PCR mastermix (K1081, Thermo Scientific™), EcoRI-HF (NEB R0101S) and HindIII-HF(NEB, R3104S), QIAprep Spin Miniprep Kit (QIAGEN, 27104), T4 DNA Ligase (5 U/μL) (Thermo Scientific™, EL0011), ReliaPrep™ DNA Clean-Up and Concentration System

(17) Heterochromatome wide analyses reveal MBD2 as a phase separation scaffold for heterochromatin compartmentalization and compositionNucleic Acids ResDecember 11, 2025Hui Zhang, Enes Ugur, Christian Hake, Hector Romero, Maria Arroyo, Marah Mahmoud, Frederik Lermyte, Heinrich Leonhardt, M Cristina Cardososequence was amplified by PCR from peMBD2G (pc2399) using primer pairs containing HindIII (NEB, cat. no.: R3104S) and SalI (NEB, cat. no.: R3138S) restriction enzyme cutting sites for the forward primer and the reverse

(18) Genome editing with the HDR-enhancing DNA-PKcs inhibitor AZD7648 causes large-scale genomic alterationsNature BiotechnologyNovember 1, 2025Grégoire Cullot, Eric J. Aird, Moritz F. Schlapansky, Charles D. Yeh, Lilly van de Venn, Iryna Vykhlyantseva, Susanne Kreutzer, Dominic Mailänder, Bohdan Lewków, Julia Klermund, et al.brief, 400–1,700 ng of gDNA was digested by HindIII-HF (20 U per reaction) and 1× rCutSmart buffer (NEB, R3104S and B6004S), followed by a 3-h incubation at 37 °C. The digested DNA was five-fold diluted in a buffer

(19) Click-code-seq reveals strand biases of DNA oxidation and depurination in human genomeNature Chemical BiologyOctober 31, 2025Vakil Takhaveev, Nikolai J. L. Püllen, Navnit K. Singh, Lucie Lefevre, Emilie A. Aghajani, Sabrina M. Huber, Stefan Schauer, Hailey L. Gahlon, Anna R. Poetsch, Shana J. Sturlaplasmid maxi prep kit (Qiagen, 12162) and digested with BamHI-HF (NEB, R3136S) and HindIII-HF (NEB, R3104S) restriction enzymes, generating sticky ends for subsequent ligation. Specifically, 20 U of each enzyme

(20) The physiological functions of the Cbp2D and Cbp2E proteins are important for insoluble cellulose-dependent growth in Cellvibrio japonicusApplied and Environmental MicrobiologyOctober 22, 2025Baily E. Kakacek, Jiabao Liang, Kyle A. Dickerson, Jeffrey G. Gardnerpurified vector was cut with EcoRI-HF and HindIII-HF restriction enzymes (catalog numbers: R3101S and R3104S, New England Biolabs) and recovered using a QIAquick PCR Purification Kit (catalog no: 28104). The PCR

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HindIII-HF

HindIII-HF from New England Biolabs

Description

Sequence

New England Biolabs

Citations (168)

Reviews

HindIII-HF from New England Biolabs

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