Deep VentDNA Polymerase from New England Biolabs

Product Specs
ItemDeep VentDNA Polymerase
CompanyNew England Biolabs
Price $114.00 Inquire
Catalog NumberM0258S
Formatunits/ml
Concentration2,000 units/ml
Quantity200 units
ApplicationsDNA Analysis
TypeDNA Polymerase
Temperature-20 C

Specifications/Features

Comments:
Item name is changed

Buffer:
1X ThermoPol Reaction Buffer:

20 mM Tris-HCl

10 mM (NH₄)₂SO₄

10 mM KCl

2 mM MgSO₄

0.1 % Triton X-100

pH 8.8 @ 25°C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Single cell transcriptional perturbome in pluripotent stem cell modelsMolecular Systems BiologyDecember 10, 2025Elisa Balmas, Maria L Ratto, Kirsten E Snijders, Silvia Becca, Carla Liaci, Irene Ricca, Giorgio R Merlo, Raffaele A Calogero, Luca Alessandrì, Sasha Mendjan, et al.Reaction Buffer (10X),New England Biolabs,#B9004S Deep Vent DNA Polymerase (2 U/µL),New England Biolabs,#M0258S Q5 Hot Start High-Fidelity DNA Polymerase (2 U/µL),New England Biolabs,#M0493 Q5 Reaction Buffer (5X),New

(2) Protocol for quantifying phase-separated condensates in living cells using HILO microscopy imaging and genetic engineeringSTAR ProtocOctober 17, 2025Axel Espinoza, Steven Ingersoll, Xiaojun RenHCl,Gibco,Cat#A11139-03 Ciprofloxacin,Sigma-Aldrich,Cat#17850 Deep Vent DNA polymerase,New England Biolabs,Cat#M0258S dTAG-13,Tocris,Cat#6605 Dulbecco’s modified Eagle’s medium (DMEM),Sigma-Aldrich,Cat#D6429 Ethylenediaminetetraacetic

(3) Mitochondria-targeted oligomeric α-synuclein induces TOM40 degradation and mitochondrial dysfunction in Parkinson’s disease and parkinsonism-dementia of GuamCell Death & DiseaseDecember 18, 2024Velmarini Vasquez, Manohar Kodavati, Joy Mitra, Indira Vedula, Dale J. Hamilton, Ralph M. Garruto, K. S. Rao, Muralidhar L. Hegdefull-length α-Syn, amplified from the pcDNA WT-α-Syn plasmid using DeepVent DNA polymerase (NEB-LABS M0258, USA) and CW WT α-Syn-FLAG F/R primer pair. This fragment was then cloned into the pCW-Cas9 vector (a

(4) An Investigation Into Carbapenem Resistance in Enterobacteriaceae Among Outpatients With Urinary Tract Infection in Southwestern UgandaCureusOctober 25, 2024Barbra Tuhamize, Deusdedit Tusubira, Charles Masembe, Pascal Bessong, Joel Bazirawithin 4 hours [8]. We purchased the PCR kit for gene amplification from New England Biolabs Inc. (M0258L Deep Vent DNA Polymerase, ThermoPol reaction buffer, and magnesium sulfate (MgSO4) solution. Each of

(5) Fluorescent Protein Variants Generated by Reassembly between Skeleton and ChromophoreACS OmegaFebruary 2, 2021Tingting Sun, Tianpeng Li, Ke Yi, Guoquan Yan, Xiaolian Gao(Sangon Biotech, Shanghai, China, Table S2). PCR reactions were performed using DeepVent polymerase (M0258L, NEB) as described in the Supporting Information Methods. The amplified products were digested with

(6) A cell-free antibody engineering platform rapidly generates SARS-CoV-2 neutralizing antibodiesbioRxiv : the preprint server for biologyOctober 30, 2020Chen X, Gentili M, Hacohen N, Regev A.Phusion DNA polymerase (New England Biolabs, M0530L) and Deep Vent DNA polymerase (New England Biolabs, M0258L) were used for both PCRs to ensure efficient amplification. PCR cycle number was chosen to avoid over-amplification

(7) Chronic exposure of a freshwater mussel to elevated p CO ₂ : Effects on the control of biomineralization and ion‐regulatory responsesEnvironmental Toxicology and ChemistryFebruary 1, 2018Jennifer D. Jeffrey, Kelly D. Hannan, Caleb T. Hasler, Cory D. SuskiµL) were 2 µL cDNA, 0.2 µM primer, and 5 µL Taq th o 5X Master Mix (Taq 5X Master Mix, catalog no. M0258L; New England BioLabs). Cycling conditions were 95 °C (30 s), 55 °C (30 s), and 68 °C (30 s) for 38 cycles

(8) An Inducible Alpha-Synuclein Expressing Neuronal Cell Line Model for Parkinson's Disease1Journal of Alzheimer's disease : JADJanuary 1, 2018Vasquez V, Mitra J, Perry G, Rao KS, Hegde ML.bluntended full-length α-Syn amplified from pcDNA-WT-α Syn plasmid using Deep Vent DNA polymerase (M0258, NEB) into a pCW-Cas9 vector (gift from Eric Lander and David Sabatini, Addgene plasmid 50661) [19]

(9) Examining Sources of Error in PCR by Single-Molecule SequencingPLOS ONEJanuary 6, 2017Vladimir Potapov, Jennifer L. OngDNA Polymerase (#M0491), Phusion High Fidelity DNA Polymerase (#M0530), Deep Vent DNA Polymerase (#M0258), and Taq DNA polymerase (#M0273) are supplied by New England Biolabs. Other polymerases were purchased

(10) Chromatin-Bound Oxidized α-Synuclein Causes Strand Breaks in Neuronal Genomes in in vitro Models of Parkinson's DiseaseJournal of Alzheimer's disease : JADJanuary 1, 2017Vasquez V, Mitra J, Hegde PM, Pandey A, Sengupta S, Mitra S, Rao KS, Hegde ML.blunt-ended full-length α-Syn amplified from pcDNA-WT-α-Syn plasmid using Deep Vent DNA polymerase (M0258, NEB) into pCWCas9 vector (gift from Eric Lander and David Sabatini, Addgene plasmid 50661) [32] and digested

(11) BB0347, from the Lyme Disease Spirochete Borrelia burgdorferi, Is Surface Exposed and Interacts with the CS1 Heparin-Binding Domain of Human FibronectinPLoS ONESeptember 27, 2013Robert A. Gaultney, Tammy Gonzalez, Angela M. Floden, Catherine A. Brissetterespectively. Recombinant Protein Production DNA sequences were amplified via PCR using DeepVent DNA Polymerase (NEB, #M0258S; Ipswich, MA) to prevent addition of the 3′ A-overhang onto amplified nucleotide segments. Primers used

(12) Identification of Signaling Protein Complexes by Parallel Affinity Precipitation Coupled with Mass SpectrometryCell Communication InsightsSeptember 11, 2013Heng Lu, Qishan Lin, Jihe Zhao(for HA-tag);1,7–10pHAN mammalian expression vector (for Myc-tag);1,11,12Deep Vent DNA polymerase (#M0258S; New England Biolabs, Ipswich, MA, USA);T4 DNA ligase (#M0202S; New England Biolabs);Aurum Plasmid Mini Kit

(13) Effective modifications for improved homologous recombination and high-efficiency generation of recombinant adenovirus-based vectorsJournal of Virological MethodsNovember 1, 2008Chengxiang Wu, Vivek R. Nerurkar, Richard Yanagihara, Yuanan Lu5′-gcatcaagaacagaagtatgtc-3′ . In this PCR amplification, Deep Vent DNA polymerase (NEB, Beverly, MA, Cat. No. M0258S) was used and the PCR product was directly ligated into pShuttle-CMV-GFP DNA that had been linearized

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