HhaI from New England Biolabs

Product Specs
ItemHhaI
CompanyNew England Biolabs
Price $67.00 Inquire
Catalog NumberR0139S
Concentration20 u/µL
Quantity2000 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

GCG/C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Screening of Hepatocellular Carcinoma in Hepatic Cirrhosis Patients by a Novel Blood-Based Multi-Omics TestTechnol Cancer Res TreatMarch 23, 2026Lei Chen, Jun Liu, Mengcheng Long, Pinghai HuThermo Fisher Scientific). Then, the samples were digested with methylation-sensitive restriction endonuclease Hha I (R0139L, New England BioLabs) at 37 °C for 30 min and at 65 °C for 20 min. Next, the UltraII DNA Library Prep

(2) Identifying Differentially Methylated Sites for Methylation-Sensitive qPCR-based NIPT of Trisomies 13 and 18Advanced Biomedical ResearchDecember 30, 2025Ali Najafi, Erfaneh Dastbaz, Artin Mehrabani, Morteza Rajabi, Ghazal Sedaghat Shayegan, Mohammad Miryounesi, Mohammad H. Modarressirestriction enzymes. The enzymes used in this study included AciI, HhaI, HpaII, and HpyCH4IV (R0551L, R0139S, R0171S, and R0619S, respectively; New England BioLabs™). This analysis was performed on DNA extracted

(3) Sequence and trajectory of early Alzheimer's disease-related tau inclusions in the hippocampal formation of cases without amyloid-β depositsActa NeuropatholMay 23, 2025Heiko Braak, Benjamin Mayer, Simone Feldengut, Michael Schön, Kelly Del TrediciR0541S, Ipswich, MA; 10U/µl), HaeII (New England BioLabs R0107S; 20U/µl), and HaeI (New England BioLabs R0139S; 20U/µl). Incubation took place at 37 °C for 3 h, heat inactivation at 80 °C for 20 min. For genotype

(4) Apolipoprotein E abundance is elevated in the brains of individuals with Down syndrome-Alzheimer's diseaseActa NeuropatholMay 19, 2025Clíona Farrell, Yazead Buhidma, Paige Mumford, Wendy E. Heywood, Jenny Hällqvist, Lisi Flores-Aguilar, Elizabeth J. Andrews, Negin Rahimzadah, Orjona Stella Taso, Eric Doran, et al.incubation at 72 ℃ for 10 min. PCR product was digested using restriction enzyme HhaI (New England Biolabs, R0139S) at 37 ℃ for 2 h 30 min, followed by 68 ℃ for 20 min. Digested PCR product of unknown and positive control

(5) Deep characterization of females with heterozygous Duchenne muscular dystrophy mutationsJ NeurolMarch 4, 2025Pietro Riguzzi, Daniele Sabbatini, Aurora Fusto, Sara Vianello, Beatrice Merlo, Vittoria Zangaro, Giuliana Capece, Domenico Gorgoglione, Gianni Sorarù, Riccardo Bariani, et al.identify the more active X chromosome. To do so, the DNA was digested with HpaII (R0171S, NEB) and HhAI (R0139S, NEB) methylation sensitive enzymes and purified by phenol/chloroform and ether extraction. An equal

(6) Apolipoprotein E abundance is elevated in the brains of individuals with Down syndrome-Alzheimer’s diseasebioRxivMarch 1, 2025Clíona Farrell, Yazead Buhidma, Paige Mumford, Wendy E. Heywood, Jenny Hällqvist, Lisi Flores-Aguilar, Elizabeth Andrews, Negin Rahimzadah, Orjona Stella Taso, Eric Doran, et al.incubation at 72 °C for 10 min. PCR product was digested using restriction enzyme HhaI (New England Biolabs, R0139S) at 37 °C for 2 h 30 min, followed by 68 °C for 20 min. Digested PCR product of unknown and positive

(7) Bisulfite‐free PCDHGB7 methylation in urine enables early noninvasive detection of urothelial carcinomaBioengineering & Translational MedicineFebruary 26, 2025Zhicong Yang, Qing Chen, Shihua Dong, Peng Xu, Zhanrui Mao, Yaping Dong, Wei Li, Wenxuan Li, Yang Han, Lihe Dai, et al.PCDHGB7 methylation in urine was quantified by MSRE‐qPCR using 100 ng gDNA digested with HhaI (NEB, R0139) and HpaII (NEB, R0171) endonuclease. GAPDH gene, absent of any MSRE cutting sites, was designed for

(8) Neurovascular Decoupling Is Associated With Lobar Intracerebral Hemorrhages and White Matter HyperintensitiesJournal of the American Heart AssociationFebruary 18, 2025Suzanne E. van Dijk, Nadieh Drenth, Anne Hafkemeijer, Gerda Labadie, Marie‐Noëlle W. Witjes‐Ané, Frank Baas, Jeroen P. Vreijling, Gerard J. Blauw, Serge A. R. B. Rombouts, Jeroen van der Grond, et al.using 5 μL PCR‐product, 5 μL rCutSmart‐Buffer and 20 U HhaI, recombinant restriction enzyme (NEB; ref. R0139L), the PCR‐product was cleaved by overnight incubation at 37° C. Products were visualized by Agarose Gel electrophoresis

(9) Re-engineered guide RNA enables DNA loops and contacts modulating repression in E. coliNucleic Acids ResearchAugust 27, 2024Yunshi Yang, Iris Rocamonde-Lago, Boxuan Shen, Ieva Berzina, Johanna Zipf, Björn Högbergdigested overnight in a 200 μl reaction containing 120 μl of the cell lysate, 480 U of HhaI (NEB, #R0139L), and 1x rCutSmart™ Buffer (NEB, #B6004S). The digestion reaction was carried out for 18 h at 37°C,

(10) Novel β-amyloid PET Imaging Study of [18F]92 in Patients with Cognitive DeclineACS OmegaAugust 13, 2024Ming Ni, Xingxing Zhu, Kaixuan Wang, Wenliang Guo, Qin Shi, Yuying Li, Mengchao Cui, Qiang Xieplatforms (Quanterix), following the manufacturer’s instructions. Using PCR amplification and HhaI (NEB, R0139S) digestion to determine the Apolipoprotein E (APOE) genotype according to the previous study.51 All

(11) A Blood Hepatocellular Carcinoma Signature Recognizes Very Small Tumor Nodules with Metastatic TraitsJournal of Clinical and Translational HepatologyJune 28, 2024Kun Chen, Junxiao Wang, Liping Jiang, Fei Zhao, Ruochan Zhang, Zhiyuan Wu, Dongmei Wang, Yuchen Jiao, Hui Xie, Chunfeng Quisolation kit. The cfDNA (5–40 ng) was digested using the methylation-sensitive restriction enzyme Hha I (R0139L, New England BioLabs) and subsequently ligated to customized MCP adapters with random DNA barcodes.

(12) Correlation of blood–brain barrier leakage with cerebral small vessel disease including cerebral microbleeds in Alzheimer's diseaseFrontiers in NeurologyFebruary 16, 2023Zhaozhao Cheng, Linbin Dai, Yan Wu, Yuqin Cao, Xianliang Chai, Peng Wang, Chang Liu, Ming Ni, Feng Gao, Qiong Wang, et al.DNA fragment using the primers F-5′GGCACGGCTGTCCAAGGA and R- 5′CTCGCGGATGGCGCTGAG. Next, HhaI (NEB, R0139S) was added to the mixture of the PCR products for restriction enzyme digestion. Finally, the reaction

(13) Molecular diagnosis of pancreatobiliary tract cancer by detecting mutations and methylation changes in bile sampleseClinicalMedicineJanuary 1, 2023Shun He, Fanxin Zeng, Huihui Yin, Pei Wang, Yinlei Bai, Qianqian Song, Jiangtao Chu, Zhen Huang, Yumeng Liu, Hong Liu, et al.technology.21 Briefly, DNA fragments were digested with the methylation-sensitive restriction enzyme HhaI (R0139S, New England Biolabs, MA, USA), which cleaves unmethylated, but not methylated DNA at the 4 bp restriction

(14) Characterization of Cme and Yme thermostable Cas12a orthologsCommunications BiologyDecember 1, 2022Ryan T. Fuchs, Jennifer L. Curcuru, Megumu Mabuchi, Audrey Noireterre, Peter R. Weigele, Zhiyi Sun, G. Brett Robbdigesting with BstXI (NEB #R0113) for 30 min at 37 °C. Substrates were also digested with HhaI (NEB #R0139), HinP1I (NEB #R0124), or FspI (NEB #R0135) to assess the ability of the method to identify 3′ overhang,

(15) Reversible epigenetic alterations regulate class I HLA loss in prostate cancerCommunications biologySeptember 1, 2022Rodems TS, Heninger E, Stahlfeld CN, Gilsdorf CS, Carlson KN, Kircher MR, Singh A, Krueger TEG, Beebe DJ, Jarrard DF, et al.DNA was digested with AluI and HhaI restriction enzymes (New England Biolabs, Cat# R0137L and Cat# R0139L) and enriched with MBD2-MBD protein (Takara Bio, Cat# 631962). Enriched DNA was subjected to qPCR with

(16) Mitosis of hepatitis B virus-infected cells in vitro results in uninfected daughter cellsJHEP ReportsSeptember 1, 2022Thomas Tu, Benno Zehnder, Jochen M. Wettengel, Henrik Zhang, Sally Coulter, Vikki Ho, Mark W. Douglas, Ulrike Protzer, Jacob George, Stephan UrbanCutSmart buffer (B7204, New England Biolabs [NEB], Ipswich, MA, USA), 7.5 U RecJf (M0264, NEB), and 10 U HhaI (R0139, NEB). The combined restriction digestion and exonuclease reaction was incubated for 4 rounds of a 15-min

(17) YAP induces an oncogenic transcriptional program through TET1-mediated epigenetic remodeling in liver growth and tumorigenesisNature geneticsAugust 1, 2022Wu BK, Mei SC, Chen EH, Zheng Y, Pan D.was confirmed by digestion with methylation sensitive restriction enzymes Hha I (New England Biolabs, R0139S) and Hpa II (New England Biolabs, R0171S) and methylation insensitive restriction enzymes Msp I (New England Biolabs

(18) Z-DNA is remodelled by ZBTB43 in prospermatogonia to safeguard the germline genome and epigenomeNature cell biologyJuly 1, 2022Meng Y, Wang G, He H, Lau KH, Hurt A, Bixler BJ, Parham A, Jin SG, Xu X, Vasquez KM, et al.We incubated 1.5 pmoles of probe DNA (CC, CL or LL) with 2 U (or 4 U when indicated) of HhaI (NEB, R0139S) restriction enzyme at 37 °C, Bsiwl (NEB, R0553S) at 55 °C or with TaiI (Thermo, ER1141) at 65 °C for

(19) Hypermethylated PCDHGB7 as a Biomarker for Early Detection of Endometrial Cancer in Endometrial Brush Samples and Cervical ScrapingsFrontiers in Molecular BiosciencesJanuary 4, 2022Jiangjing Yuan et al.For each digestion reaction, 100 ng gDNA was taken as input, and every two units of the HhaI (NEB, R0139) and HpaII (NEB, R0171) were added, making the final volume 25 μL, followed by digestion at 37°C for

(20) A sensitive and Specific PCR-based Assay to Quantify Hepatitis B Virus Covalently Closed Circular (ccc) DNA While Preserving Cellular DNABIO-PROTOCOLApril 20, 2021Benno Zehnder, Stephan Urban, Thomas Tu3125000036) 20. 10× Cutsmart buffer (NEB, catalog number: B7204, store at -20 °C) 21. HhaI (NEB, catalog number: R0139, store at -20 °C) 22. PCR-grade H2O (e.g., B. Braun Melsungen, for injection purposes) 23. RecJf (NEB,

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HhaI

HhaI from New England Biolabs

Sequence

New England Biolabs

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HhaI from New England Biolabs

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