Normal Goat Serum from Cell Signaling Technology

Product Specs
ItemNormal Goat Serum
CompanyCell Signaling Technology
Price $87.00 Inquire
Catalog Number5425S
Quantity10 ml
ApplicationsIHC-P, IF-F, IF-IC
DiseaseNormal
TypeSerum
Source/Expression SystemGoat
Research AreaRelated Products

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Cell Signaling Technology

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Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

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Website: http://www.cellsignal.com/

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(1) CircSMAD4 shapes matrix-remodeling TAMs in lung adenocarcinomaNon-coding RNA ResearchAugust 1, 2026Zhengwei Yu, Xinyue Wang, Yiqian Zheng, Yifan He, Jiayu Lin, Yue Xiao, Bin Mo, Haoyu Xie, Sitong Hang, Xia Gao, et al.Cat# PK001), and nonspecific binding was blocked with normal goat serum blocking solution (CST, Cat# 5425S). Sections were incubated with primary antibodies against Ki-67 (Servicebio, Cat# GB111499), E-cadherin

(2) Schizophrenia risk gene ZNF804A controls ribosome localization and synaptogenesis in developing human neuronsSci AdvMay 22, 2026Laura Sichlinger, Maximilian Hausherr, Sara Guerrisi, Lucia Dutan-Polit, George Chennell, Roland Nagy, Rugile Matuleviciute, Fatema Nasser, Szidonia Farkas, Rosemary A. Bamford, et al.permeabilization and blocking solution containing Triton X-100 and 2% normal goat serum (Cell Signaling: 5425S) for 1 hour at room temperature. Primary antibodies were applied overnight at 4°C, followed by washing

(3) Targeting PIP5K ameliorates hepatic cancer by inhibiting PI3K/AKT and the autophagy mechanism and enhancing ROS-mediated apoptosisFront PharmacolMay 8, 2026P. A. Shantanu, Bishal Rajdev, N. P. Syamprasad, Jagadeesh Kumar Gangasani, Arijit Mandal, Samir Ranjan Panda, Ramakrishna Sistla, Sai Balaji Andugulapati, Shiv Kumar Sarin, Dinesh Mani Tripathi, et al.13054); anti-rabbit HRP-conjugate secondary antibody (cat no. 7074S); and normal goat serum (cat no. 5425) were purchased from Cell Signaling Technologies (CST, Massachusetts, United States). Beclin-1 (cat

(4) Poldip2 deficiency attenuates lung disease severity in a mouse model of COVID-19PLoS OneApril 29, 2026Ruinan Hu, Alejandra Valdivia, Taylor White, Willy Ju, Maegan L. Brockman, Zhan Zhang, Hongyan Qu, Georgette Gafford, Giji Joseph, Samantha Burton, et al.sections were blocked with 2% IgG-free BSA (001-000-162, Jackson ImmunoResearch) and 5% normal goat serum (5425S, Cell Signaling) in PBS for 1 hour at room temperature. Primary antibodies against Poldip2 (rabbit,

(5) Linking Mitochondrial Dysfunction to the Immune Microenvironment in HFpEF: An Integrated Bioinformatics and Experimental ApproachImmun Inflamm DisApril 22, 2026Yingying Xie, Haoming He, Yike Li, Qiang Chen, Yanxiang Gao, Jingang Zhengdeparaffinization, antigen retrieval with citrate buffer (pH 6.0), and blocking with normal goat serum (CST, 5425). Subsequently, they were incubated with primary antibodies targeting EFHD1 (dilution 1:100, Abmart)

(6) Zinc-induced exposure of an LxL motif drives clathrin-mediated endocytosis of the zinc transceptor ZIP4Journal of Biological ChemistryApril 17, 2026Tianqi Wang, Chi Zhang, Yao Zhang, Majid Jafari, Kenneth M. Merz, Jian HuCat#9002-93-1) and blocked for 1 h in DPBS containing 5% goat serum (Cell Signaling Technology, Cat#5425S), followed by incubation with Alexa Fluor 568–conjugated goat anti-mouse IgG (1:500; Thermo Fisher Scientific,

(7) Dispensable players: N-WASP and WASP are not crucial for homology-directed DNA repairEMBO ReportsApril 10, 2026Thu Han Le Phan, Anders Buchard, Cord Brakebuschtemperature, followed by permeabilization and blocking in PBS containing 5% normal goat serum (Cell Signaling, 5425) and 0.3% Triton X-100 for 1 h. Cells were then processed for EdU detection, if required, or incubated

(8) Age-dependent H3K9 trimethylation by dSetdb1 impairs mitochondrial UPR leading to degeneration of olfactory neurons and loss of olfactory function in DrosophilaElifeMarch 26, 2026Francisco Muñoz-Carvajal, Nicole Sanhueza, Mario Sanhueza, Felipe A Courtwith PBS with 0.1% Triton X-100, and blocked for 1 hr with Normal goat Serum (NGS) (Cell Signaling, 5425 S) at 5% in PBS, 0.1% Triton X-100 and stained overnight at 4 °C with primary and after three washes

(9) A comparative analysis of CD70-directed CAR-T cells for glioblastoma treatment demonstrates a superior efficacy of the ligand-based constructMolecular Therapy OncologyMarch 19, 2026Alexandros Kourtesakis, Hiu Nam Hannah Chow, Eileen Bailey, Sandra Horschitz, Ammar Jabali, Rainer Will, Christoph Schifflers, Abigail K. Suwala, Hannah Rohdjess, Melissa Hahn, et al.supplemented with 0.25% (w/v) bovine serum albumin (BSA; #T844.2, Roth) and 10% (v/v) normal goat serum (#5425, Cell Signaling Technology) at RT. Next, sections were incubated O/N at 4°C with the following primary

(10) Potential treatment benefits of a GLP‑1R antagonist in combination with immune checkpoint inhibitors in colorectal cancerOncology LettersFebruary 10, 2026Zengqin Zhan, Chuncha Zhan, Lianqiao Li, Dan Lan, Guya Meng, Fan ChenX-100 in PBS for 10 min at room temperature. Blocking was performed with 5% normal goat serum (cat. no. 5425; Cell Signaling Technology, Inc.) for 30 min at room temperature and the sections were incubated overnight

(11) CDK2 inhibition promotes neuronal differentiation in neuroblastomaSci RepFebruary 6, 2026Mohammad Alzrigat, Loay Mahmoud, Ada Nursel Topçu, Marta Valenti, Lu Zhang, Puck Veen, Fabian John, Wesam Bazzar, Kaisa Lehti, Lars-Gunnar Larssonwith PBS, and blocked for 1 h in blocking buffer consisting of 0.1% Triton X-100 and 5% goat serum (5425 S; Cell Signaling Technology) in PBS. For immunostaining, cells were incubated overnight at 4 °C with

(12) Distinct contributions of Etv2+ and Flk1+ progenitors to endothelial, hematopoietic, and cardiac lineagesCell ReportsJanuary 27, 2026Dereck Alleyne, Minseo Kim, Jun Wu, Yoojung Kwon, Ye-Ram Kim, Ashraf Ul Kabir, Matthew Ishahak, Jeffrey R. Millman, Changxu Fan, Hyung Joo Lee, et al.Bovine Serum Albumin,Cell Signaling Technology,Cat# 9998 Normal Goat Serum,Cell Signaling Technology,Cat# 5425 "Fetal bovine serum, embryonic stem cell-qualified",R&D Systems,Cat# S10250 L-Glutamine,ThermoFisher Scientific

(13) Characterizing molecular and behavioral changes arising from ROMK potassium channel deficiency in the cerebellumFrontiers in Behavioral NeuroscienceJanuary 26, 2026Jacopo Agrimi, Aidan J. Dunphy, Justin C. Zhong, Wenxi Zhang, Naeem Sbaiti, Daniel R. Turner, Lucia Bernardele, D. Brian Foster, Brian O’Rourke, Nazareno Paolocci, et al.temperature in a solution containing 10% normal goat serum (NGS, Cell Signaling Technology, catalog number: 5425) and 0.05% Triton X-100 in PBS. Following this step, sections were incubated with primary antibodies

(14) Intestinal epithelial TLR5 signaling promotes barrier-supportive macrophagesScience ImmunologyJanuary 23, 2026Ming-Ting Tsai, Ryann Callaghan, Charles Ng, Lisette Peres-Tintin, Dean B. Matthews, Nikketa Stanford, Karuna Ganesh, Mary K. Estes, Gretchen E. DiehlO.C.T. compound and then blocked with DPBS containing 5% normal goat serum (Cell Signaling Technology, #5425S), and 0.3% Triton X-100 (Fisher Bioreagents, #BP150-100) for 1 hour at room temperature (RT). Slides

(15) Myelin is repaired by constitutive differentiation of oligodendrocyte progenitorsScienceJanuary 22, 2026Yevgeniya A. Mironova, Brendan Dang, Dongeun Heo, Yu Kang T. Xu, Angela Yu-Huey Hsu, Jaime Eugenin Eugenin von Bernhardi, Gian Carlo Molina-Castro, Anya A. Kim, Jing-Ping Lin, Daniel S. Reich, et al.10 min, then incubated in blocking solution containing 10% normal goat serum (NGS, Cell signaling, 5425S) + 0.3% Triton-X in 1x PBS for 1 hour at RT on a shaker. For ASPA staining, an additional 10-minute

(16) Oncostatin M orchestrates collective epithelial migration via HIF1A activationbioRxivJanuary 16, 2026Ian C. McLean, Sean M. Gross, Tiera A. Liby, Mark A. Dane, Daniel S. Derrick, Laura M. Heiserligand treatment. Cells were blocked for 1 hour in a PBS solution (5% Normal Goat Serum (Cell Signaling #5425), 0.3% Triton X (Thermo Fisher Scientific #85111), then incubated overnight with the primary conjugated

(17) Transcriptomic Analysis in a Model of Aniridia-Associated Keratopathy for Target Discovery and Evaluation of Duloxetine TherapyInvestigative Ophthalmology & Visual ScienceJanuary 16, 2026Dina Javidjam, Petros Moustardas, Ava Dashti, Daniel Aberdam, Arnaud Schweitzer-Chaput, Salvatore Cisternino, Dominique Bremond-Gignac, Neil Lagalistaining. Sections were blocked with BSA (Cat. no. A7906; Sigma-Aldrich Corp.) and goat serum (Cat. no. CST-5425S; Cell Signaling Technology, Danvers, MA, USA) to prevent nonspecific binding, incubated with primary

(18) Vibration‐Mediated Recovery of Irradiated Osteocytes and Their Regulatory Role in Breast Cancer Bone MetastasisAdvanced Healthcare MaterialsJanuary 1, 2026Xin Song, Kimberly Seaman, Amel Sassi, Chun‐Yu Lin, Tiankuo Chu, Liyun Wang, Yu Sun, Lidan Youand 5% CO2. Cells were fixed with 4% formaldehyde for 15 min and blocked with 5% normal goat serum (5425, Cell Signaling) and 0.3% Triton X‐100 in PBS for 1 h. They were then incubated overnight at 4 °C with

(19) Mycobacteria trehalose dimycolate interactions with host Mincle remodel blood-brain barrier junctions for brain invasionCell ReportsDecember 23, 2025Megan I. Hayes, Sumedha Ravishankar, Jonathan K. Shanahan, Adam J. Fountain, Lalita Ramakrishnan, Cressida A. Madigan1 mL PBSTx, and blocked with 1 mL blocking solution made with 10% normal goat serum (Cell Signaling 5425S) and 1% bovine serum albumin (Sigma, #A9647) in PBSTx for 5 h at room temperature. Larvae were then

(20) Phosphatidylserine receptors TIM-1 and AXL mediate tick-borne Powassan virus entryiScienceDecember 19, 2025Maria Daskou, Anne K. Zaiss, Arjit Vijey Jeyachandran, Kari-Ann Takano, Ryan L. Kan, Ramya Paravastu, Ephrem Gerald, Nivedha Satheeshkumar, Jennifer Rios-Rodriguez, Brandy Russell, et al.Normal Donkey Serum,Jackson ImmunoResearch,Cat#017-000-121 Normal Goat Serum,Cell Signaling Technology,Cat#5425S Fc Receptor Blocker,INNOVEX biosciences,Cat#NB309-30 Trizol,Thermo Fisher Scientific,Cat#1596018 ,, Experimental models

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Normal Goat Serum from Cell Signaling Technology

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Normal Goat Serum from Cell Signaling Technology

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