Monarch DNA Gel Extraction Kit from New England Biolabs

Product Specs
ItemMonarch DNA Gel Extraction Kit
CompanyNew England Biolabs
Price Supplier Page View Company Product Page
Catalog NumberT1020L
Quantity250 preps
ApplicationsDNA Manipulation
SourceReaction, Gel
Nucleic Acid TypeDNA Fragment, PCR Product
Temperature25 C
Brand/TrademarkNew England Biolabs Inc (NEB)

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Functional and antigenic characterization of SARS-CoV-2 spike fusion peptide by deep mutational scanningbioRxivNovember 30, 2023Ruipeng Lei, Enya Qing, Abby Odle, Meng Yuan, Timothy J.C. Tan, Natalie So, Wenhao O. Ouyang, Ian A. Wilson, Tom Gallagher, Stanley Perlman, et al.instruction, followed by purification using the Monarch Gel Extraction Kit (New England Biolabs, catalog no. T1020L). The FPPR mutant library PCR product was introduced into SARS-CoV-2 BAC encoding Wuhan-Hu-1 sequence

(2) Characterisation of a Live-Attenuated Rabies Virus Expressing a Secreted scFv for the Treatment of RabiesVirusesJuly 31, 2023Samuel P. Smith, Rebecca Shipley, Pascal Drake, Anthony R. Fooks, Julian Ma, Ashley C. Banyardmanufacturer’s instructions and products isolated using either NEB Monarch® DNA Gel Extraction Kit (T1020L) or NEB Monarch® PCR & DNA Cleanup Kit (T1030L). To generate cDNA from viral RNA extraction, the QIAGEN

(3) Transcription activator-like effector protects bacterial endosymbionts from entrapment within fungal hyphaeCurrent BiologyJuly 10, 2023Ingrid Richter, Philipp Wein, Zerrin Uzum, Claire E. Stanley, Jana Krabbe, Evelyn M. Molloy, Nadine Moebius, Iuliia Ferling, Falk Hillmann, Christian Hertweckcommercial assays,Critical commercial assays ,, Monarch® DNA Gel Extraction Kit,New England Biolabs,CAT#T1020L NEBuilder® HiFi DNA Assembly Cloning Kit,New England Biolabs,CAT#E2621L MasterPure™ DNA Purification

(4) A self‐propagating, barcoded transposon system for the dynamic rewiring of genomic networksMolecular Systems BiologyJune 12, 2023Max A English, Miguel A Alcantar, James J CollinsSciences,2160250 DNeasy Blood & Tissue Kit,Qiagen,69504 Monarch DNA Gel Extraction Kit,New England Biolabs,T1020L Magnetic Separation Rack,New England Biolabs,S1515S CLARIOstar Plus,BMG Labtech, SpectraMax M5 plate

(5) Protocol to develop a synthetic biology toolkit for the non-model bacterium R. palustrisSTAR ProtocolsJune 1, 2023Mark Kathol, Cheryl Immethun, Rajib Sahaassays,Critical commercial assays ,, Monarch DNA Gel Extraction Kit,New England Biolabs® Inc.,CAT#T1020L PureLink™ Quick Plasmid Miniprep Kit,Thermo Fisher Scientific,CAT#K210011 TURBO DNA-free™ Kit,Invitrogen™,CAT#AM1907 TaqMan®

(6) Functionally distinct promoter classes initiate transcription via different mechanisms reflected in focused versus dispersed initiation patternsThe EMBO JournalMay 15, 2023Leonid Serebreni, Lisa‐Marie Pleyer, Vanja Haberle, Oliver Hendy, Anna Vlasova, Vincent Loubiere, Filip Nemčko, Katharina Bergauer, Elisabeth Roitinger, Karl Mechtler, et al.2014",https://bioconductor.org/packages/release/bioc/html/DESeq2.html Other,Other,Other Mini‐PROTEAN Tetra Vertical Electrophoresis Cell,Bio‐Rad,Cat#1658004 Monarch Gel Extraction,NEB,Cat#T1020L Illumina Truseq small RNA library prep kit,Illumina,Cat#RS‐200‐0012 Power Blotter Station,Thermo Fisher,Cat#PB0010 MaxCyte

(7) Cis-regulatory atlas of primary human CD4+ T cellsBMC GenomicsMay 11, 2023Kurtis Stefan, Artem Barski#28104) and size selected for 150–500 bp fragments on a 2% agarose gel, using 300 mg per column (NEB #T1020L). The eluted ATAC-Seq library was cloned into AgeI- and SalI-digested pLenti-STARR using a 3:1 molar

(8) Evolved bacterial resistance to the chemotherapy gemcitabine modulates its efficacy in co-cultured cancer cellseLifeFebruary 3, 2023Serkan Sayin, Brittany Rosener, Carmen G Li, Bao Ho, Olga Ponomarova, Doyle V Ward, Albertha J M Walhout, Amir Mitchella 3% agarose gel, and the product was extracted using NEB Monarch DNA Gel Extraction Kit (NEB, Cat# T1020L). Next, we used Agilent High Sensitivity DNA Kit (Agilent Technologies, Cat# 5067-4626) to evaluate

(9) Mutational fitness landscape of human influenza H3N2 neuraminidaseCell ReportsJanuary 31, 2023Ruipeng Lei, Andrea Hernandez Hernandez Garcia, Timothy J.C. Tan, Qi Wen Teo, Yiquan Wang, Xiwen Zhang, Shitong Luo, Satish K. Nair, Jian Peng, Nicholas C. WuPureLink HiPure Plasmid Miniprep Kit,Thermo Fisher Scientific,Cat#K210003 Monarch DNA Gel Extraction Kit,New England Biolabs,Cat#T1020L Superscript III reverse transcriptase,Thermo Fisher Scientific,Cat#18080044 Deposited data,, Raw

(10) Genome-wide screen in human plasma identifies multifaceted complement evasion of Pseudomonas aeruginosaPLOS PathogensJanuary 25, 2023Manon Janet-Maitre, Stéphane Pont, Frerich M. Masson, Serena Sleiman, Julian Trouillon, Mylène Robert-Genthon, Benoît Gallet, Chantal Dumestre-Perard, Sylvie Elsen, Christine Moriscot, et al.400 bp were selected by extraction from a 2% agarose gel using a Monarch DNA Gel Extraction Kit (NEB#T1020L). Purified adaptor-DNA fragments were amplified by Phusion polymerase (NEB#M0530) in combination with

(11) Simple Wastewater Preparation Protocol Applied to Monitor the Emergence of the Omicron 21L/BA.2 Variant by Genome SequencingVirusesJanuary 17, 2023Nathalie Wurtz, Maelle Boussier, Louis Souville, Gwilherm Penant, Alexandre Lacoste, Philippe Colson, Bernard La Scola, Sarah AherfiWaltham, MA, USA). The Monarch® DNA Gel Extraction Kit (New England BioLabs, Ipswich, MA, USA; ref T1020L, Evry-Courcouronnes, France) was used for the purification of an interest band at 400 pb with an elution

(12) Insights into the secondary and tertiary structure of the Bovine Viral Diarrhea Virus Internal Ribosome Entry SiteRNA BiologyDecember 31, 2022Devadatta Gosavi, Iwona Wower, Irene K. Beckmann, Ivo L. Hofacker, Jacek Wower, Michael T. Wolfinger, Joanna Sztuba-Solinskafinal libraries were purified on 2% preparative agarose gel by Monarch DNA Gel Extraction kit (NEB, T1020L). The concentration of each library was measured by performing quantitative PCR (qPCR) using PerfeCTa®

(13) Probing the biophysical constraints of SARS-CoV-2 spike N-terminal domain using deep mutational scanningScience AdvancesNovember 25, 2022Wenhao O. Ouyang, Timothy J.C. Tan, Ruipeng Lei, Ge Song, Collin Kieffer, Raiees Andrabi, Kenneth A. Matreyek, Nicholas C. Wuinstruction, followed by purification using the Monarch Gel Extraction Kit (New England Biolabs, catalog no. T1020L). The final insert and the linearized vector were digested by BsmBI-v2 (New England Biolabs, catalog

(14) Interpreting and de-noising genetically engineered barcodes in a DNA virusPLOS Computational BiologyNovember 22, 2022Sylvain Blois, Benjamin M. Goetz, James J. Bull, Christopher S. Sullivan# T1030L) before gel purification using the Monarch DNA gel extraction kit (New England Biolabs, # T1020L). The DNA concentration of each sample was determined using the QUBIT dsDNA BR assay kit (ThermoFisher,

(15) Characterization of multiple interactions between the envelope E protein of SARS-CoV-2 and human BRD4STAR ProtocolsOctober 28, 2022Mohamad Zandian, Suk Min Jang, Catherine Lachance, Arpan Acharya, Siddappa N. Byrareddy, Jacques CÃ´tÃ©, Tatiana G. KutateladzeMotif,Cat # 81093 KOD polymerase,EMD Millipore,Cat # 71086-4 Monarch DNA gel extraction,NEB,Cat # T1020L BamHI,NEB,Cat # R3136 XhoI,NEB,Cat # R0146 CIP (Calf Intestin Phosphatase),NEB,Cat # M0290 T4 DNA Ligase

(16) The miR-15a/16-1 and miR-15b/16-2 clusters regulate early B cell development by limiting IL-7 receptor expressionFrontiers in ImmunologyAugust 25, 2022Katharina Hutter, Thomas Rülicke, Tamas G. Szabo, Lill Andersen, Andreas Villunger, Sebastian HerzogSupplementary Table S1 for details), purified by agarose gel isolation (Monarch DNA Gel Extraction Kit, NEB (T1020L)) and ligated into LMP-destGFP (22) 3′ of the destabilized GFP cDNA via XhoI and ClaI. The pre-B cell

(17) SARS-CoV-2 Testing of Aircraft Wastewater Shows That Mandatory Tests and Vaccination Pass before Boarding Did Not Prevent Massive Importation of Omicron Variant into EuropeVirusesJuly 9, 2022Lorlane Le Targa, Nathalie Wurtz, Alexandre Lacoste, Gwilherm Penant, Priscilla Jardot, Alexandre Annessi, Philippe Colson, Bernard La Scola, Sarah Aherfiaccording to the supplier protocol using the Monarch DNA Gel Extraction Kit (New England BioLabs, ref T1020L, Évry-Courcouronnes, France) with a final elution volume of 40 µL. For passengers’ samples, cDNA was

(18) Efficient suppression of endogenous CFTR nonsense mutations using anticodon-engineered transfer RNAsMolecular Therapy - Nucleic AcidsJune 14, 2022Wooree Ko, Joseph J. Porter, Matthew T. Sipple, Katherine M. Edwards, John D. Lueckrestriction enzymes from NEB, gel purifications were performed using the Monarch DNA Gel Extraction Kit (NEB T1020L), and minipreps were performed using the Monarch Plasmid Miniprep Kit (NEB T1010L). NEB 5-alpha competent

(19) A conserved sequence in the small intracellular loop of tetraspanins forms an M-shaped inter-helix turnScientific reportsMarch 16, 2022Reppert N, Lang T.8.3). The wanted bands were cut out and cleaned up using the Monarch DNA Gel Extraction Kit (NEB, #T1020L). The linear plasmid was phosphorylated using T4 Polynucleotide kinase (NEB, #M0201S) and ligated by

(20) dCas9-based gene editing for cleavage-free genomic knock-in of long sequencesNature cell biologyFebruary 1, 2022Wang C, Qu Y, Cheng JKW, Hughes NW, Zhang Q, Wang M, Cong L.(Thermo Scientific, F-548L). The PCR products were purified using a gel extraction kit (New England BioLabs, T1020L) following the manufacturer’s instructions. A poly(A) tail was added to the purified products using

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(1)

3/13/2020Very Quick and Easy Protocol with This Gel Extraction Kit Xuewei WangRecommended for freshmen performing DNA purification.
(1)

2/18/2020Purification of DNA from Agarose GelDr. Thippeswamy SannasiddappaIt is just OK to have in the lab if you are not worried too much about the quality of DNA.