SNAP-Surface Alexa Fluor 647 from New England Biolabs

Product Specs
ItemSNAP-Surface Alexa Fluor 647
CompanyNew England Biolabs
Price $419.00 Inquire
Catalog NumberS9136S
Quantity50 nmol
ApplicationsCellular Biology
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Site-specific ribozyme-mediated alkylation of DNA substratesNucleic Acids ResMarch 19, 2026Yuanlin He, Timothy J Wilson, Jie Deng, Yuhang Luo, Kaizhou Yan, Yuting Xie, David M J Lilley, Lin HuangMTR1 ribozyme, 10 μM ssDNA substrate, and 50 μM SNAP-Surface® Alexa Fluor® 647 (New England Biolabs S9136S). The mixture was incubated at 37°C for 12 h. All subsequent steps—including reaction termination, ethanol

(2) MYC modulates TOP2A diffusion to promote substrate detection and activityNature CommunicationsFebruary 9, 2026Donald P. Cameron, Kathryn Jackson, Alessia Loffreda, Carl Möller, Vladislav Kuzin, Matteo Mazzocca, Evanthia Iliopoulou, Hallgerdur Kolbeinsdottir, Andrej Paluda, Evgeniya Pavlova, et al.N-terminal SNAP-tagged MYCWT and MYC200–320 were labeled in 5 μM SNAP-surface Alexa Fluor 647 (NEB, S9136S) at 37 °C for 30 min before being dialyzed into 20 mM Tris pH 8, 100 mM KCl, 1 mM DTT, 1 mM EDTA, 20%

(3) FCHo2, not talin, enables inside-out activation of integrin ɑvβ5 in curved adhesionsNature CommunicationsFebruary 6, 2026Chih-Hao Lu, Christina E. Lee, Wei Zhang, Yang Yang, Luis A. Valencia, He You, Ching-Ting Tsai, Bianxiao Cuipre-warmed 1X DMEM medium supplemented with 1 µM O6-benzylguanine (BG)-coupled Alexa Fluor 647 (NEB, #806 S9136S) at 37 °C with 5% CO2. After 15-min incubation, the samples were washed 5 times with a pre-warmed 1X

(4) Super-Resolution Imaging of Nuclear Pore Responses to Mechanical Stress and Energy DepletionVirusesJanuary 27, 2026Dariana Torres-Rivera, Sobhan Haghparast, Bernd Rieger, Gregory B. Melikyanwere blocked with 20% FBS for 30 min and stained with 0.5 µM SNAP-Surface AF647 (New England Biolabs #S9136S, Ipswich, MA, USA) with 1 µM of dithiothreitol (DTT, American Bioanalytical #AB00490, Natick, MA, USA)

(5) Optimizing Effective Labeling Efficiency in MINFLUX 3D DNA-PAINT Microscopy by Maximizing Marker Detection ProbabilityACS PhotonicsJanuary 7, 2026Christian Soeller, Alexandre F. E. Bokhobza, Javier Casares-Arias, Alexander H. Clowsleyachieved by incubating the sample for 1 h at RT with a 1 μM solution of SNAP-Surface Alexa-647 (NEB, S9136S) diluted in PBS supplemented with 0.5% BSA (Sigma, A9647) and 1 mM DTT (Sigma, D9779). A final round

(6) Reinforced optical cage systems enable drift-free single-molecule localization microscopyCommunications EngineeringDecember 15, 2025Hao Qiu, Matthew C. Tang, Selene K. Roberts, Guoli Li, Rong Su, Marisa L. Martin-Fernandez, David T. Clarke, Shugang Liu, Xiaojie Liu, Lin WangI36933, Thermo Fisher) for 30 min and then stained with 1 μM SNAP-Surface Alexa Fluor 647 (Catalog No. S9136S, New England Biolabs) dissolved in PBS with 0.5% (w/v) BSA and 1 mM DTT. Sample preparation for STORM Prior

(7) FCHo2, instead of talin, enables inside-out activation of integrin ɑvβ5 in curved adhesionsbioRxivDecember 2, 2025Chih-Hao Lu, Christina E. Lee, Wei Zhang, Yang Yang, Luis A. Valencia, He You, Ching-Ting Tsai, Bianxiao Cuipre-warmed 1X DMEM medium supplemented with 1 μM O6-benzylguanine (BG)-coupled Alexa Fluor 647 (NEB, #806 S9136S) at 37 °C with 5% CO2. After 15-min incubation, the samples were washed 5 times with a pre-warmed 1X

(8) Super-resolution microscopy reveals a Rab6a-dependent trafficking hub for rhodopsin at the mammalian rod photoreceptor GolgiBiology OpenNovember 15, 2025Maryam Hekmatara, Samantha L. Thompson, Kristen N. Haggerty, Sydney Hagen, Brooke A. Brothers, Bali Daniels, Guillaume Luxardi, Ala Moshiri, Wen-Tao Deng, Michael A. Robichaux925-32210). Other labeling reagents include SNAP-Surface Alexa Fluor 647 [New England Biolabs (NEB), Cat# S9136S], SNAP-Cell® TMR-Star (NEB, Cat# S9105S) and DAPI (Thermo Fisher Scientific, Cat# 62248). DNA plasmids The

(9) Octopamine signaling regulates the intracellular pattern of the presynaptic active zone scaffold within Drosophila mushroom body neuronsPLOS BiologyOctober 23, 2025Hongyang Wu, Sayaka Eno, Kyoko Jinnai, Ayako Abe, Kokoro Saito, Yoh Maekawa, Darren W. Williams, Nobuhiro Yamagata, Shu Kondo, Hiromu Tanimotobrains. For SNAP or CLIP chemical tagging, brains were incubated in SNAP-Surface 647 (1:1000; NEB; S9136S) or CLIP-Surface 647 (1:1000; NEB; S9234S) in 0.3% PBT for 15 min and washed 3 × 20 min with 0.1% PBT.

(10) A phosphoinositide switch from PI(4,5)P2 to PI4P triggers endocytosis by inducing dynamin-mediated fission in secretory cellsSci AdvOctober 17, 2025Xiaoli Guo, Lisi Wei, Nidhi Kundu, Wonchul Shin, Chung Yu Chan, Sue Han, Ammar Mohseni, Maryam Molakarimi, Min Sun, Xin-Sheng Wu, et al.temperature for 50 min with SNAP substrate dye solution containing 1 μM SNAP-Surface Alexa Fluor 647 (NEB, S9136S), 0.5% bovine serum albumin, and 1 mM dithiothreitol (DTT), resulting in the attachment of SNAP-surface Alexa Fluor 647

(11) Posttranslational microtubule modification alters podocalyxin-trafficking in epithelial cellsFrontiers in Cell and Developmental BiologySeptember 22, 2025Lena-Sophie Gorek, Dominik Trauth, Natalia Kamm, Lana Sophie Schiffke, Carlotta Zerbian, Lina-Marie Mende, Ralf JacobSNAP-Surface Alexa Fluor 546 (S9132S),"New England Biolabs (Ipswich, United States)" SNAP-Surface Alexa Fluor 647 (S9136S),"New England Biolabs (Ipswich, United States)" SNAP-Surface Block (S9143S),"New England Biolabs (Ipswich,

(12) Scalable and lightweight deep learning for efficient high accuracy single-molecule localization microscopyNat CommunAugust 5, 2025Yue Fei, Shuang Fu, Wei Shi, Ke Fang, Ruixiong Wang, Tianlun Zhang, Yiming Lilabeling, cells were incubated in a dye solution containing 1 μM SNAP-tag ligand BG-AF647 (catalog no. S9136S, New England Biolabs), 1 mM DTT (catalog no. 1111GR005, BioFroxx), and 0.5% (w/v) bovine serum albumin

(13) Molecular mapping and functional validation of GLP-1R cholesterol binding sites in pancreatic beta cellsElifeApril 24, 2025Affiong Ika Oqua, Kin Chao, Liliane El Eid, Lisa Casteller, Billy P Baxter, Alba Miguéns-Gómez, Sebastian Barg, Ben Jones, Jorge Bernardino de la Serna, Sarah L Rouse, et al.SNAP/FLAG-hGLP-1R WT or V229A cells were seeded onto glass bottom MatTek dishes and labelled with SNAP-Surface 647 (S9136S, New England Biolabs) for 15 min at 37 °C in full media, washed, and imaged in RMPI-1640 media without

(14) Direct effect of membrane environment on the activation of mGluR2 revealed by single-molecule FRETStructureApril 3, 2025Chiranjib Banerjee, Brandon Wey-Hung Liauw, Reza Vafabakhshgroup,99-50-3 SNAP-Surface 549,New England Biolabs,S9112S SNAP-Surface Alexa Fluor 647,New England Biolabs,S9136S DDM,Anatrace,Cas# 69227-93-6 Mal (11.2),Avanti,Cas# 1219008-64-6 LMNG,Anatrace,Cas# 1257852-96-2 OGNG,Anatrace,Cas#

(15) Conditional Control of Benzylguanine Reaction with the Self-Labeling SNAP-tag ProteinBioconjugate ChemistryMarch 19, 2025Steven E. Caldwell, Isabella R. Demyan, Gianna N. Falcone, Avani Parikh, Jason Lohmueller, Alexander Deiterspenicillin-streptomycin) spinning at 1400 rpm for 2 min. Cells were then stained with 1 μM SNAP surface dye-AF647 (Cat# S9136S, New England Biolabs) for 1 h at 37 °C in a cell culture incubator. Cells were washed twice with FACS

(16) Structural mechanisms for centrosomal recruitment and organization of the microtubule nucleator γ-TuRCNat CommunMarch 12, 2025Qi Gao, Florian W. Hofer, Sebastian Filbeck, Bram J. A. Vermeulen, Martin Würtz, Annett Neuner, Charlotte Kaplan, Maja Zezlina, Cornelia Sala, Hyesu Shin, et al.secondary antibody for 30 min and subsequently incubated with 2.5 μM SNAP-Surface® Alexa Fluor® 647(S9136S, New England Biolabs) for 1 hr at RT. Samples were washed once with PBS. Subsequently, the PBS solution

(17) Impact of physiological ionic strength and crowding on kinesin-1 motilityCell Structure and FunctionFebruary 18, 2025Misaki Sagawa, Kazuhiro Oiwa, Hiroaki Kojima, Ken’ya Furuta, Keitaro ShibataUSA). For fluorescent labeling, K430-3 × SNAP and SNAP-Surface® Alexa Fluor® 647 (New England Biolabs: S9136S; Ipswich, MA, USA) were mixed 1:10 and incubated at room temperature for 60 min. The buffer was replaced

(18) Direct observation of fluorescent proteins in gels: A rapid, cost-efficient, and quantitative alternative to immunoblottingBiol CellFebruary 9, 2025Matthieu Sanial, Ryan Miled, Marine Alves, Sandra Claret, Nicolas Joly, Véronique Proux-Gillardeaux, Anne Plessis, Sébastien Léon#11836170001)] in the presence or absence of SNAP‐surface Alexa fluor 647 substrate (New England Biolabs, # S9136S) at a final concentration of 1.66 µM. LDS sample buffer was added (1X final) and samples were denatured

(19) Sequential recruitment of F-BAR proteins controls cytoskeletal crosstalk at the yeast bud neckCurrent BiologyFebruary 3, 2025Joseph O. Magliozzi, Lucas A. Runyan, Priyanka Dutta, Gregory J. Hoeprich, Bruce L. Goode,New England Biolab,S9112S SNAP-Surface Biotin 549,New England Biolab,N/A SNAP-Surface 647,New England Biolab,S9136S Alexa Fluor 488 Phalloidin,ThermoFisher,A12379 VECTASHIELD Antifade Mounting Media,Vector Laboratories,H-1000-10 2

(20) Engineered protein G variants for multifunctional antibody-based assembliesProtein SciJanuary 25, 2025Tomasz Slezak, Kelly M. O'Leary, Jinyang Li, Ahmed Rohaim, Elena K. Davydova, Anthony A. KossiakoffSNAP‐GLM were conjugated with BG‐Alexa Fluor 647 and BG‐Alexa Fluor 488 (New England Biolabs, Cat # S9136S, S9129S) respectively, according to the manufacturer's protocol. Excess substrate was removed with PD MiniTrap G‐25

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10/21/2024Excellent SnapTag Ligand for Labelling Protein-SnapTag Fusions for Confocal Imaging Studies Pavan Kumar ChodisettiTo understand the role of a cell surface protein in cell morphogenesis during the bacterial cell cycle, I aimed to observe the localization pattern of my protein of interest. For this purpose, I ... Read Review