SNAP-Surface Alexa Fluor 546 from New England Biolabs

Product Specs
ItemSNAP-Surface Alexa Fluor 546
CompanyNew England Biolabs
Price $419.00 Inquire
Catalog NumberS9132S
Quantity50 nmol
ApplicationsCellular Biology
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) MYC modulates TOP2A diffusion to promote substrate detection and activityNature CommunicationsFebruary 9, 2026Donald P. Cameron, Kathryn Jackson, Alessia Loffreda, Carl Möller, Vladislav Kuzin, Matteo Mazzocca, Evanthia Iliopoulou, Hallgerdur Kolbeinsdottir, Andrej Paluda, Evgeniya Pavlova, et al.buffer. C-terminal SNAP-tagged TOP1 was labeled by labeled in 5 μM SNAP-surface Alexa Fluor 546 (NEB, S9132S) at 37 °C for 30 min. Excess fluorophore was removed through dialysis for 24 h into 100 mM KCl, 20 mM

(2) Posttranslational microtubule modification alters podocalyxin-trafficking in epithelial cellsFrontiers in Cell and Developmental BiologySeptember 22, 2025Lena-Sophie Gorek, Dominik Trauth, Natalia Kamm, Lana Sophie Schiffke, Carlotta Zerbian, Lina-Marie Mende, Ralf Jacob(A22283),"Thermo Fisher Scientific (Waltham, United States)" substrate,provider SNAP-Surface Alexa Fluor 546 (S9132S),"New England Biolabs (Ipswich, United States)" SNAP-Surface Alexa Fluor 647 (S9136S),"New England Biolabs

(3) SH2-mediated steric occlusion of the C2 domain regulates autoinhibition of SHIP1 inositol 5-phosphatasebioRxivSeptember 11, 2025Emma E. Drew, Hunter G. Nyvall, Matthew A.H. Parson, Reed K. Talus, John E. Burke, Scott D. HansenFor labeling, Btk-SNAP was combined with a 1.5x molar excess of SNAP-Surface Alexa546 dye (NEB, Cat# S9132S) and incubated overnight at 4°C. The next day, Btk-SNAP-AF546 was desalted into buffer containing 20

(4) Spatial Gradient of Axon Terminal Maturation Reveals the Sequential Active Zone Assembly in Adult Drosophila Mushroom BodiesMicroPubl BiolAugust 15, 2025Hongyang Wu, William Constance, Ayako Abe, Shu Kondo, Darren Williams, Hiromu TanimotoRRID: AB_2534072). For SNAP chemical tagging, samples were incubated in SNAP-Surface 546 (1:10000; NEB; S9132S) in 0.3% PBT for 15 min and washed 3×20 min in 0.1% PBT. Samples are mounted on microscope slides using

(5) Subcellular activation of β-adrenergic receptors using a spatially restricted antagonistProceedings of the National Academy of SciencesOctober 1, 2024Federica Liccardo, Johannes Morstein, Ting-Yu Lin, Julius Pampel, Di Lang, Kevan M. Shokat, Roshanak Irannejadalbumin (BSA) and dry milk powder are from Research Products International. SNAP-Surface Alexa Fluor 546 (S9132S), SNAP-Cell TMR-Star (S9105S), and SNAP-Cell 647 SiR (S9102S) are from New England Biolabs. Heparin

(6) Single-molecule imaging reveals the mechanism of bidirectional replication initiation in metazoaCellJuly 25, 2024Riki Terui, Scott E. Berger, Larissa A. Sambel, Dan Song, Gheorghe Chistol(HRV) 3C Protease,this paper,N/A Sortase,Sparks et al. 37,N/A SNAP-Surface Alexa Fluor 546,NEB,Cat# S9132S SNAP-Surface Alexa Fluor 647,NEB,Cat# S9136S Alexa Fluor 546 C5 Maleimide,Thermo Fisher Scientific,Cat#

(7) Dissecting the molecular basis for the modulation of neurotransmitter GPCR signaling by GINIPStructureJanuary 1, 2024Alex Luebbers, Alberto J. Gonzalez-Hernandez, Myles Zhou, Stephen J. Eyles, Joshua Levitz, Mikel Garcia-Marcosfor 30 min at 37°C with 1 μM SNAP-Surface Alexa Fluor 546 non-permeable dye (New England Biolabs; #S9132S) in a saline solution containing, in mM: 135 NaCl, 5.4 KCl, 10 HEPES, 2 CaCl2, 1 MgCl2. Coverslip was

(8) Selective activation of intracellular β1AR using a spatially restricted antagonistbioRxivNovember 27, 2023Federica Liccardo, Johannes Morstein, Ting-Yu Lin, Julius Pampel, Kevan M. Shokat, Roshanak Irannejadalbumin (BSA) and dry milk powder are from Research Products International. SNAP-Surface Alexa Fluor 546 (S9132S), SNAP-Cell TMR-Star (S9105S), SNAP-Cell 647 SiR (S9102S) are from New England Biolabs. Heparin solution

(9) Molecular basis for differential activation of p101 and p84 complexes of PI3Kγ by Ras and GPCRsCell ReportsMarch 28, 2023Manoj K. Rathinaswamy, Meredith L. Jenkins, Benjamin R. Duewell, Xuxiao Zhang, Noah J. Harris, John T. Evans, Jordan T.B. Stariha, Udit Dalwadi, Kaelin D. Fleming, Harish Ranga-Prasad, et al.we combined 1 mL of 20 μM protein with 25 μM Alexa 546-SNAP surface dye (New England Biolabs, Cat# S9132S). SNAP dye labeling was performed in buffer containing 20 mM HEPES [pH 7], 200 mM NaCl, 10% glycerol,

(10) Potent microtubule-depolymerizing activity of a mitotic Kif18b–MCAK–EB networkJournal of Cell ScienceMarch 1, 2023Toni McHugh, Julie P. I. WelburnAlexa Fluor647,NEB,Cat. #: S9136S,Chemical fluorophore other,SNAP-Surface Alexa Fluor546,NEB,Cat. #: S9132S,Chemical fluorophore other,Catalase from Bovine liver,Sigma-Aldrich,Cat# : C40,enzyme other,Glucose

(11) High hedgehog signaling is transduced by a multikinase-dependent switch controlling the apico-basal distribution of the GPCR smoothenedeLifeSeptember 9, 2022Marina GonÃ§alves Antunes, Matthieu Sanial, Vincent Contremoulins, Sandra Carvalho, Anne Plessis, Isabelle Becam,New England Biolabs,Cat#S9124S,3.3 µM "Chemical compound, drug",SNAP-Surface Alexa Fluor 546,New England Biolabs,Cat#S9132S,3.3 µM "Chemical compound, drug",SNAP-Surface Block,New England Biolabs,Cat#S9143S,2 µM "Chemical

(12) Genome-wide CRISPR screen identified a role for commander complex mediated ITGB1 recycling in basal insulin secretionMolecular metabolismSeptember 1, 2022Yang L, Fye MA, Yang B, Tang Z, Zhang Y, Haigh S, Covington BA, Bracey K, Taraska JW, Kaverina I, et al.5′-TAACAACTGTGGTCGCTGCGAAGTATGAGGGA-3’. SNAP substrates were purchased from NEB (SNAP-Surface® Block, S9143S; SNAP-Surface® Alexa Fluor® 546, S9132S; SNAP-Surface® Alexa Fluor® 647, S9136S). 2.2 Lentivirus production HEK293FT cells (a gift frorm David

(13) Synergistic phase separation of two pathways promotes integrin clustering and nascent adhesion formationeLifeJanuary 20, 2022Lindsay B Case, Milagros De Pasquale, Lisa Henry, Michael K Rosen,Alexa Fluor 568 C5 Maleimide,Invitrogen,Cat#A20341, "Chemical compound, drug",SNAP-Surface Alexa Fluor 546,New England BioLabs,Cat#S9132S, "Chemical compound, drug",SNAP-Surface Alexa Fluor 488,New England BioLabs,Cat#S9129S, "Chemical

(14) A fine-tuned azobenzene for enhanced photopharmacology in vivoCell Chemical BiologyNovember 18, 2021Vanessa A. Gutzeit, Amanda Acosta-Ruiz, Hermany Munguba, Stephanie HÃ¤fner, Arnaud Landra-Willm, Bettina Mathes, JÃ¼rgen Mony, Dzianis Yarotski, Karl BÃ¶rjesson, Conor Liston, et al.GTP,MilliporeSigma™,Cat#G8877-25MG biocytin,MilliporeSigma™,Cat#B4261-250MG SNAP-Surface Alexa Fluor 546,New England Biolabs,Cat#S9132S Lipofectamine 2000,Invitrogen,Cat#11668027 Trypsin,Sigma-Aldrich,Cat# T1763 DMEM/F12,Sigma-Aldrich,Cat#

(15) Colloidal structure and proton conductivity of the gel within the electrosensory organs of cartilaginous fishesiScienceSeptember 24, 2021Molly Phillips, Alauna C. Wheeler, Matthew J. Robinson, Valerie Leppert, Manping Jia, Marco Rolandi, Linda S. Hirst, Chris T. Amemiya104586; http://n2t.net/addgene:104586; RRID:Addgene_104586) SNAP Alexa Fluor-546,New England Biolabs,Cat#S9132S MS-222 (Tricaine),"Western Chemical, Inc.",Cat#NC0872873 Proteinase K,ThermoFisher,Cat#EO0492 "DAPI

(16) GIPR antagonist antibodies conjugated to GLP-1 peptide are bispecific molecules that decrease weight in obese mice and monkeysCell reports. MedicineMay 18, 2021Lu SC, Chen M, Atangan L, Killion EA, Komorowski R, Cheng Y, Netirojjanakul C, Falsey JR, Stolina M, Dwyer D, et al.,Cell Signaling Technology,Cat#5589; RRID:AB_10693925 SNAP-Surface® Alexa Fluor® 546,New England Biolabs,Cat#S9132S SNAP-Surface® Alexa Fluor® 647,New England Biolabs,Cat#S9136S Mouse monoclonal anti-human IgG Fc,Amgen,Clone

(17) Mechanisms of differential desensitization of metabotropic glutamate receptorsCell reportsApril 27, 2021Abreu N, Acosta-Ruiz A, Xiang G, Levitz J.,Thermo Fisher Scientific,Cat#10437028 SNAP-Surface Alexa Fluor 546 (BG-Alexa 546),New England Biolabs,Cat#S9132S SNAP-Surface Alexa Fluor 488 (BG-Alexa 488),New England Biolabs,Cat#S9232S Lipofectamine 2000 Transfection Reagent

(18) Combined kinesin-1 and kinesin-3 activity drives axonal trafficking of TrkB receptors in Rab6 carriersDevelopmental cellFebruary 22, 2021Zahavi EE, Hummel JJA, Han Y, Bar C, Stucchi R, Altelaar M, Hoogenraad CC.Bio,635056 Lipofectamine 2000,Thermo Fisher,11668019 Surface-SNAP-Alexa-488,NEB,S9129S Surface-SNAP-Alexa-546,NEB,S9132S Surface-SNAP-Alexa-647,NEB,S9136S SYLGARD 184 Silicone Elastomer Kit,Dow,N/A ,, Experimental Models:

(19) Genome-wide alterations of uracil distribution patterns in human DNA upon chemotherapeutic treatmentseLifeSeptember 21, 2020Hajnalka L Pálinkás et al.synthase inhibitor, treatment: 100 nM, 48 hr" "Chemical compound, drug",SNAP-Surface Alexa Fluor 546,NEB,S9132S,SNAP substrate for superresolution imaging "Chemical compound, drug",SNAP-Surface Alexa Fluor 647,NEB,S9136S,SNAP

(20) RNA-Induced Conformational Switching and Clustering of G3BP Drive Stress Granule Assembly by CondensationCellApril 16, 2020Guillén-Boixet J, Kopach A, Holehouse AS, Wittmann S, Jahnel M, Schlüßler R, Kim K, Trussina IREA, Wang J, Mateju D, et al.AppliChem,Cat#A2760 Polyethylene glycol 20000,Merck,Cat#817018 SNAP-Surface Alexa Fluor 546,New England Biolabs,Cat#S9132S T4 RNA ligase 1,New England Biolabs,Cat#M0204S pCp-Cy5,Jena Bioscience,Cat#NU-1706-CY5 Lambda Protein

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