FokI from New England Biolabs

Product Specs
ItemFokI
CompanyNew England Biolabs
Price $308.00 Inquire
Catalog NumberR0109L
Concentration4 u/µL
Quantity5000 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

GGATG(9/13)

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Integrative Single-Cell Epigenomic Atlas Annotates the Regulatory Genome of the Adult Mouse BrainbioRxivFebruary 7, 2026Zhaoning Wang, Songpeng Zu, Ethan J. Armand, Timothy H. Loe, Jonathan A. Rink, Wanying Wu, Yang Xie, Lei Chang, Chenxu Zhu, Nicholas D. Johnson, et al.and RNA sublibraries For each DNA sublibrary tube, 1 μL SbfI-HF (NEB, R3642L) and 1 μL FokI (NEB, R0109L) were added. For each RNA sublibrary tube, 1 μL NotI-HF (NEB, R3189L) was added. The restriction enzyme digestion

(2) Association of Apolipoprotein C-III Gene Polymorphisms (rs2854116 and rs2854117) with Susceptibility to Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD) in a Turkish PopulationMedicina (Kaunas)August 18, 2025Damla Karaagac, Suat Morkuzu, Naci Senkal, Ersel Bilgin, Yasemin Oyacı, Tufan Tükek, Sacide Pehlivan, Alpay Medetalibeyoglurs2854116 variant, and MspI enzyme was used for the rs2854117 variant (New England Biolabs, cat no: R0109S, R0106T, respectively) (New England Biolabs, Ipswich, MA, USA). Restriction products were run on 3.5%

(3) Exploring the Influence of VDR Genetic Variants TaqI, ApaI, and FokI on COVID-19 Severity and Long-COVID-19 SymptomsJournal of Personalized MedicineNovember 28, 2023Ghayda’ Alhammadin, Yazun Jarrar, Abdalla Madani, Su-Jun LeeBiotechnology (Seongnam, Republic of Korea). For the PCR-RFLP analysis, the restriction enzymes FokI (cat. no. R0109S) and TaqI-v2 (cat. no. R0149S) were obtained from New England Biolabs (Ipswich, MA, USA); ApaI (cat.

(4) Status of Vitamin D Receptor Gene Polymorphism and 25-Hydroxy Vitamin D Deficiency with Essential HypertensionIndian Journal of Clinical BiochemistryJuly 1, 2022M. Prasad, D. Rajarajeswari, P. Aruna, K. Ramalingam, R. Viswakumar, Nusrath Fathima, Sandeep Kumar Vishwakarma, Aleem Ahmed Khanof PCR mixture of VDR gene amplicons was subjected to RFLP using 0.4 µL of Fok 1 restriction enzyme (R0109S, NEB) in a reaction mixture of 25 µL with 2.5 µL reaction buffer and 2.1 µL of molecular biology grade

(5) Enhanced Detection of Viral RNA Species Using FokI-Assisted Digestion of DNA Duplexes and DNA/RNA HybridsAnalytical ChemistryMay 10, 2022Juan R. Tejedor et al.#B7204), 50 nM (1 pmol) of the duplex or heteroduplex substrate, and either 5 units of the RE FokI (NEB, #R0109S) or 10 units of BanI (NEB, #R0118S) in a final volume of 20 μl. Further details of this experimental

(6) Imaging cytoplasmic lipid droplets in vivo with fluorescent perilipin 2 and perilipin 3 knock-in zebrafisheLifeAugust 13, 2021Meredith H Wilson, Stephen C Ekker, Steven A Farberembryos, and cutting efficiency of each pair was assessed by monitoring the loss of either FokI (NEB R0109) or HphI (NEB R0158) digestion due to TALEN nuclease activity. Nuclease activity was higher for plin2

(7) Joint profiling of histone modifications and transcriptome in single cells from mouse brainNature MethodsMarch 1, 2021Chenxu Zhu, Yanxiao Zhang, Yang Eric Li, Jacinta Lucero, M. Margarita Behrens, Bing Renrespectively. Add 2.5 µL 10X Cutsmart buffer [NEB, M7204S], 1 µL SbfI-HF [NEB, R3642] and 1 µL FokI [NEB, R0109S] and 3.5 µL H2O to DNA-tube. Add 2 µL 10X Cutsmart buffer and 1 µL NotI-HF [NEB, R3189] to RNA-tube.

(8) 25-hydroxyvitamin D deficiency, exacerbation frequency and human rhinovirus exacerbations in chronic obstructive pulmonary diseaseBMC Pulmonary MedicineDecember 1, 2012Jennifer K Quint, Gavin C Donaldson, Nancy Wassef, John R Hurst, Michael Thomas, Jadwiga A Wedzichapolymorphism (rs2228570), a RFLP analysis was carried out by using the FokI restriction endonuclease enzyme (R0109S New England BioLabs). The reaction consisted of; 2μl buffer, 7.5μl dH2O, 0.5μl RE (FokI) and 10μl PCR

(9) Preparation of Group I Introns for Biochemical Studies and Crystallization Assays by Native Affinity PurificationPLoS ONEAugust 27, 2009Quentin Vicens, Anne R. Gooding, Luis F. Duarte, Robert T. Bateyexperiments were transcribed by run-off transcription from a plasmid linearized using the FokI enzyme (NEB #R0109S), as described above, but for 4 h at 37°C and in 10 mL-reaction mixes. The RNAs were ethanol precipitated

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FokI

FokI from New England Biolabs

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New England Biolabs

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FokI from New England Biolabs

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