Fnu4HI from New England Biolabs

Product Specs
ItemFnu4HI
CompanyNew England Biolabs
Price $75.00 Inquire
Catalog NumberR0178S
Concentration5 u/µL
Quantity200 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

GC/NGC

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Renalase inhibition defends against acute and chronic β cell stress by regulating cell metabolismMolecular MetabolismMay 1, 2025Tara L. MacDonald, Birgitta Ryback, Jéssica Aparecida da Silva Pereira, Siying Wei, Bryhan Mendez, Erica P. Cai, Yuki Ishikawa, Meagan Arbeau, Gordon Weir, Susan Bonner-Weir, et al.as listed on Jackson Labs). The PCR product was incubated with restriction enzyme Fnu4HI (NEB cat. R0178) for >4hrs at 37C and run on a 3% gel. Akita heterozygotes have bands at 140 and 280bp; WT mice at 140bp. 4.1.2

(2) An antireductant approach ameliorates misfolded proinsulin-induced hyperglycemia and glucose intolerance in male Akita miceGeroScienceSeptember 19, 2024Dwight A. L. Mattocks, Naidu B. Ommi, Virginia L. Malloy, Sailendra N. NichenametlaDNA was digested with the restriction enzyme Fnu4HI (New England Biolabs, Ipswich, MA, Catalog No. R0178S). Genotypes were determined based on the number and size of DNA fragments obtained after 2% agarose gel electrophoresis

(3) Compression-dependent microtubule reinforcement enables cells to navigate confined environmentsNature Cell BiologySeptember 1, 2024Robert J. Ju, Alistair D. Falconer, Christanny J. Schmidt, Marco A. Enriquez Martinez, Kevin M. Dean, Reto P. Fiolka, David P. Sester, Max Nobis, Paul Timpson, Alexis J. Lomakin, et al.created by restriction digestion using KpnI (New England Biolabs, R3142) and NotI (New England Biolabs, R0178) of pENTR1a–GFP-N2 (FR1) (a gift from E. Campeau and P. Kaufman; Addgene plasmid #19364; RRID:Addgene_19364)

(4) Loss of CFTR function is associated with reduced bitter taste receptor-stimulated nitric oxide innate immune responses in nasal epithelial cells and macrophagesFrontiers in ImmunologyJanuary 18, 2023Ryan M. Carey, James N. Palmer, Nithin D. Adappa, Robert J. Leesamples from cells that were being cultured; cDNA was digested with FNu4HI (New England Biolabs; cat # R0178S) for 1hr at 37°C, and run on an agarose gel. The presence of bands at 364 bp and 531 bp demonstrated

(5) Poorly-Controlled Type 1 Diabetes Mellitus Impairs LH-LHCGR Signaling in the Ovaries and Decreases Female Fertility in MiceYonsei medical journalJuly 1, 2019Lee J, Lee HC, Kim SY, Cho GJ, Woodruff TK.Thermo Fisher Scientific, Waltham, MA, USA) and was followed by digestion with the restriction enzyme Fnu4HI (R0178, New England Biolabs, Ipswich, MA, USA) at 37℃ overnight. PCR products of 140 base pairs (bp) and 280

(6) Association of hOGG1 Ser326Cys, ITGA2 C807T, TNF-A -308G>A and XPD Lys751Gln polymorphisms with the survival of Malaysian NPC patientsPLoS ONEJune 18, 2018Eng-Zhuan Ban, Munn-Sann Lye, Pei Pei Chong, Yoke-Yeow Yap, Siew Ying Crystale Lim, Hejar Abdul Rahman

(7) Integrin α1/Akita double-knockout mice on a Balb/c background develop advanced features of human diabetic nephropathyKidney InternationalJune 1, 2012Ling Yu, Yan Su, Paisit Paueksakon, Huifang Cheng, Xiwu Chen, Hongtao Wang, Raymond C. Harris, Roy Zent, Ambra Pozziantisense 5′-TGGTCCCACATATGCACATG-3′ primers, the PCR products were digested with Fnu4HI enzyme (NEB, R0178s) at 37C overnight and run onto 2% agarose gels (Sigma, St. Louis, MO) for detection of the wild-type

(8) A Sensitized Screen of N-ethyl-N-nitrosourea–Mutagenized Mice Identifies Dominant Mutants Predisposed to Diabetic NephropathyJournal of the American Society of NephrologyJanuary 1, 2007Elena E. Tchekneva et al.30 s, 61°C for 30 s, and 72°C for 30 s for 40 cycles. Restriction analysis with Fnu4HI enzyme (NEB, R0178s) at 37°C overnight allowed differentiation of the wild-type (140 bp) and the mutant (280 bp) alleles

(9) HPC2/ELAC2 polymorphisms and prostate cancer risk: analysis by age of onset of diseaseBritish Journal of CancerOctober 7, 2002J C Meitz et al.were described by Rebbeck et al (2000). The PCR product was digested overnight with 2 U Fnu4HI (NEB, R0178L) at 378C. The digested 495 bp PCR product was visualised as either 250+110 bp fragment (Ala/Ala genotype)

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Fnu4HI

Fnu4HI from New England Biolabs

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Fnu4HI from New England Biolabs

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