Description
Nerve Terminal Staining Kits contain Biotium's nerve terminal dyes and background reducing agents for washing away or quenching extracellular dye fluorescence. Kits are available with SynaptoGreen™ C4 (equivalent to FM®1-43), the fixable green nerve terminal dye AM1-43, or SynaptoRed™ C2 (equivalent to FM®4-64). ADVASEP-7 is a sulfonated β-cyclodextrin derivative that has been reported to reduce background fluorescence when using SynaptoGreen C4 (equivalent to FM®1-43) to stain brain slices. SCAS (4-Sulfonato calix[8]arene, sodium salt) is a quencher developed by Biotium to reduce background fluorescence. Unlike ADVASEP-7, SCAS dramatically lowers background fluorescence as soon as it is added to the preparation without the need for repeated wash steps. Sulforhodamine 101 has been used as a quencher dye to reduce SynaptoGreen™ C4 fluorescence. Nerve Terminal Staining Kit Components Nerve Terminal Staining Kit I (70030): SynaptoGreen™ C4 (5 x 1 mg ) ADVASEP-7 (250 mg) Nerve Terminal Staining Kit II (A) (70031): AM1-43 (1 mg) ADVASEP-7 (100 mg) Nerve Terminal Staining Kit II (B) (70031-1): AM1-43 (1 mg) SCAS (100 mg) Nerve Terminal Staining Kit III (70032): SynaptoGreen™ C4 (5 x 1 mg) Sulforhodamine 101 (100 mg) Nerve Terminal Staining Kit IV (70034): SynaptoRed™ C2 (5 x 1 mg) ADVASEP-7 (250 mg)
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