iQue® 3 High-Throughput Screening Cytometry Platform from Sartorius

Product Specs
ItemiQue® 3 High-Throughput Screening Cytometry Platform
CompanySartorius
Price Get Pricing Get Quote for this Product
Catalog NumberiQue3
QuantityEA
ApplicationsHigh-Throughput Screening (HTS) by Cytometry
Absorbance / Excitation405 nm, 488 nm, 561 nm, 640 nm
Excitation Wavelength405 nm, 488 nm, 561 nm, 640 nm
Measuring Range15 parameters / 13 fluorescence channels
CapacityMicroplates (96-well, 384-well, 1536-well)
Data Analysis35,000 events/sec
Dimensions(WxDxH) 99 x 63 x 66 cm
Channels8 or 15
Plate Formats96-well and 384-well
Throughput35,000 events/second, < 5 minutes/96 wells, < 20 minutes/384 wells
DetectionUp to 15 detection channels
Lasers2 or 3 lasers

Specifications/Features

Configurations: The iQue® 3 comes in multiple optical configurations: iQue® 3 (BR), iQue® 3 (VBR), iQue® 3 (VYB)

Enhanced Rinse Station:
48 hour uninterrupted run time before replacing reagent cartridges
Automated vortexing just prior to sampling for improved Q.C. results

Dynamic Range: > 7 Decades

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Local Office

Sartorius

5 Orville Drive

Bohemia, NY 11716

United States

Company Profile

International Office

Sartorius

Otto-Brenner-Straße 20

37079 Göttingen

Germany

Phone: +49 551 308 0

Company Profile

Website: www.sartorius.com

(1) Antigen-directed single domain antibody-based TNFR1 agonists elicit preferential killing of HER2-overexpressing cancer cellsiScienceApril 17, 2026Laura Unmuth, Britta Lipinski, Alicia Hoerr, Julia Harwardt, Enrico Guarnera, Michal Szczepek, Stefan Becker, Andreas Menrad, Patrick Scheerer, Andreas Evers, et al.cell staining and incubated for 15 min at room temperature protected from light. Cells were analyzed using the iQue3 system (Sartorius). Single cells as well as viable cells were gated and overlay histogram plots for

(2) Affinity-optimized TROP2 antibodies support potent antitumor activity in antibody–drug conjugatesAntibody TherapeuticsMarch 13, 2026Aiko Yamaguchi, Junping Hong, Leike Li, Kiyotaka Kobayashi, Chisato M Yamazaki, Summer Y Y Ha, Yasuaki Anami, Wei Xiong, Junquan Liu, Zhiqiang An, et al.(Thermo Fisher Scientific) at 4 °C for 1 h. Following three washes, stained cells were analyzed by flow cytometry on an iQue3 instrument (Sartorius), and the data were evaluated with FlowJo software X 10.0.7 (BD Biosciences). Western blot

(3) An intranasal adenoviral-vectored vaccine protects against highly pathogenic avian influenza H5N1 in naive and antigen-experienced animalsCell Reports MedicineFebruary 17, 2026Baoling Ying, Kelly Pyles, Tamarand L. Darling, Kuljeet Seehra, Truc Pham, Lin-Chen Huang, Houda H. Harastani, Ashish Sharma, Pritesh Desai, Elena A. Kashentseva, et al.the dark. After two washes with FACS buffer, beads were resuspended in FACS buffer and acquired on an iQue3 flow cytometer (Sartorius). The mean fluorescent intensity (MFI) was recorded. The geometric MFI of

(4) Enhancement of gemcitabine toxicity and specificity through PI3K/Akt/Nrf2 pathway inhibition in pancreatic cancerFrontiers in PharmacologyFebruary 16, 2026Yu-Shan Chen, Stephen O’Hagan, Philip J. R. Day(640922, BioLegend) and PI in the dark for 30 min at RT. The samples were analysed through an Intellcyt iQue3 flow cytometer (Sartorius) with emission filters of 530 nm for FITC (green) and 615 nm for PI (red).

(5) Drivers of clinical resistance to venetoclax and hypomethylating agents in acute myeloid leukemia and strategies for improving efficacyHemaSphereJanuary 11, 2026Ida Vänttinen, Joseph Saad, Tanja Ruokoranta, Sari Kytölä, Guangrong Qin, Bahar Tercan, Pia Ettala, Anu Partanen, Marja Pyörälä, Johanna Rimpiläinen, et al.Median fluorescence intensity (MFI) of each intracellular marker was measured using flow cytometry (iQue3, Sartorius, Germany) from AML blasts gated as shown in Supporting Information: Figure S1. MFIs were

(6) Discovery and characterization of two anti-PD-1 antibodies with a unique binding mechanism to human PD-1mAbsDecember 31, 2025Keyla María Gómez Castellano, Alejandra Montes Luna, Gregorio de Jesús Carballo Uicab, Frida Daniela Ramírez Villedas, Luis Javier Elizarrarás Rodríguez, Said Kayum Vázquez Leyva, Stefany Daniela Rodríguez Luna, Edith González González, María Martha Pedraza Escalona, Ben Holland, et al.Jose, CA, USA). Binding to hPD-1 by Intellicyt® Binding to hPD-1 was evaluated using the Intellicyt® iQue3 system (Sartorius; Göttingen, Germany) as follows. Biotinylated hPD-1 (Acro Biosystems Cat. No. PD-1-H82E4)

(7) Engineered antibodies that stabilize drug-modified KRASG12C neoantigens enable selective and potent cross-HLA immunotherapyNat CommunDecember 17, 2025Lorenzo Maso, Sarah A. Mosure, Sergio A. Rodriguez-Aponte, Angelina Pizzo, Diamond N. Mensah, Matthew Southard, Samantha Sze, Tanvir Ahmed, Brian Vash, Takamitsu Hattori, et al.1:500, BioLegend, 316305). Flow cytometry data were collected using a BD FACSymphony A3 or an IntelliCyt iQue3 flow cytometer (Sartorius). The signal intensity of PE was normalized to the values in the absence of

(8) mRNA-1010 influenza vaccine elicits distinct and enhanced humoral immunity compared to adjuvanted inactivated vaccinesnpj VaccinesDecember 15, 2025Paulina Kaplonek, Roger Vargas, Jessica Shih-Lu Lee, Harry Bertera, Eleanor Astley, Bethany Girard, Jaap Oostendorp, Carole Henry, Robert Paris, Wen-Han Yu, et al.at 37 °C. After overnight incubation, cells were fixed with 4% PFA. Fluorescence was acquired on an iQue3 (Sartorius). A phagoscore was calculated as: (%bead+ THP1 * GeoMean bead+ cells)/10,0000. Antibody-dependent

(9) A high-throughput platform for biophysical antibody developability assessment to enable AI/ML model trainingMAbsDecember 2, 2025Ammar Arsiwala, Rebecca Bhatt, Lood van Niekerk, Porfirio Quintero-Cadena, Xiang Ao, Adam Rosenbaum, Aanal Bhatt, Alexander Smith, Yaoyu Yang, K C Anderson, et al.F(ab′)2 AF488 (H10120; Invitrogen). Beads were washed again with PBSB and analyzed via flow cytometry (iQue3; Sartorius) to measure median fluorescence intensity (MFI). MFI values were normalized between 0 and

(10) Collateral hypersensitivity between ZY19489 and piperaquine neutralizes PfCRT-mediated drug efflux and Plasmodium falciparum resistanceResearch SquareOctober 9, 2025David Fidock, Tolla Okombo, Tarrick Ndiaye, Igor M. R. Qahash, Igor Gil-Iturbe, Laura M. Gil-Iturbe, Jessica L. Bridgford, Vinicius Bridgford, Vinicius Bonatto, Tomas Ward, et al.MitoTracker Deep Red FM staining (Life Technologies) and subsequent flow-cytometric analysis (IntelliCyt iQue3, Sartorius). IC50 values were derived from growth inhibition data using nonlinear regression (Prism

(11) Improving dual targeting selectivity in T-cell engagers via synapse-gated and affinity-tuned trispecific antibody designMAbsOctober 8, 2025Peng Zhao, John Schardt, Chi-I Chiang, Pooja Shah, Gee Sung Eun, Jan Martinek, Matthew Cyr, Yoshimi Johnson, Bismark Amofah, Xiaoying Ye, et al.were washed three times before resuspension in FACS buffer with DAPI and subsequently analyzed on an iQUE3 flow cytometer (Sartorius). Cellular binding study Experiments were conducted using a previously established

(12) The Long-term Effects of Acute Total-Body Irradiation on Pre-irradiation Measles-vaccine-induced Immunological MemoryRadiation ResearchOctober 1, 2025Erica L. Stover, Michelle L. Rock, John D. Olson, George W. Schaaf, Thomas H. Oguin, J. Mark Cline, Andrew N. MacintyreCytoPerm/Wash and then stored in 4% paraformaldehyde (Pierce) in PBS (Invitrogen). Cells were analyzed using a iQue3 flow cytometer (Sartorius) running ForeCyte v7.1 (Sartorius). Data were gated using FlowJo v.10.9 (BDBiosciences).

(13) CUSP06, a Novel CDH6-Targeted Antibody-Drug Conjugate, Demonstrates Antitumor Efficacy in Multiple CDH6-Expressing Human Cancer ModelsPharmaceuticsAugust 13, 2025Wei Lu, Jing Shi, Wentao Zhang, Nicole Covino, Amy Penticoff, Robert Phillips, John Cogswell, Laurie Tatalick, Stephanie Pasas-Farmer, Jianjian Zhang, et al.amount of GPF-positive expressed cells and GFP-negative cells was determined by flow cytometry (FASC, iQue3, Sartorius, Göttingen, Germany). For flowcytometry cell gating, cell population was first gated by FSC-H

(14) A Machine Learning-Based Strategy Predicts Selective and Synergistic Drug Combinations for Relapsed Acute Myeloid LeukemiaCancer ResearchJuly 15, 2025Yingjia Chen, Liye He, Aleksandr Ianevski, Kristen Nader, Tanja Ruokoranta, Nora Linnavirta, Juho J. Miettinen, Markus Vähä-Koskela, Ida Vänttinen, Heikki Kuusanmaki, et al.APC/Fire 750 Annexin V (BioLegend) and DRAQ7 (BD Biosciences). The cells were then analyzed with an iQue3 flow cytometer (Sartorius). Remaining live cells after drug treatments were gated using Forecyt (Sartorius).

(15) An mRNA vaccine encoding five conserved Group A Streptococcus antigensNat CommunJuly 1, 2025Nichaela Harbison-Price, Ismail Sebina, Rhiannon A. Bolton, Meredith Finn, Amanda J. Cork, Isabel G. Courtney, Steven Hancock, Ruby Pelingon, Johanna Richter, Olivia Ericsson, et al.µL CytoFixTM buffer (BD Biosciences) for 30 min and washed by FACS for flow cytometry assessment by iQUE3 (Sartorius). mRNA-LNP preparation and in vitro expression For each vaccine antigen, T7 RNA polymerase-mediated

(16) Structural basis for plasticity in receptor engagement by an encephalitic alphavirusCellMay 29, 2025Saravanan Raju, Sathvik Palakurty, Alan Sariol, Ngan Wagoner, Lucas J. Adams, Sean Hui, William B. Klimstra, Daved H. Fremont, Michael S. Diamond4’4,6-diamidino-2-phenylindole (DAPI, 1 μg/mL) to stain dead cells and subjected to flow cytometry analysis using an iQue3 flow cytometer (Sartorius). Ectopic expression and stable cell line generation. K562 cells stably

(17) Systems serology-based comparison of humoral immune responses induced by liposome or aluminum hydroxide adjuvanted SARS-CoV-2 spike proteinSci RepMay 28, 2025Soo Ji Kim, Taewoo Kim, Satyanarayana Bejjani, Mi Sun Kim, Jung Hyuk Lee, Yuna Shin, Sun-Je Woo, Beom Min Cheon, Doyoung Kim, Sumin Lee, et al.the washing stations. The PE MFI for each sample was obtained using a high-throughput flow cytometer (iQue3, Sartorius). The results are presented as the average MFI of duplicate tests for each sample. A high-throughput

(18) Comparing functional and genomic-based precision medicine in blood cancer patientsHemasphereApril 24, 2025Lukas Kazianka, Alexander Pichler, Christiane Agreiter, Johannes Rohrbeck, Christoph Kornauth, Edit Porpaczy, Christian Sillaber, Wolfgang R. Sperr, Karoline V. Gleixner, Alexander Hauswirth, et al.the addition of DAPI (BioLegend, Lot B205177) to the staining panel. The assay plates were read on an iQue3 HT flow cytometer screener (Sartorius). The data were first gated to remove noise, doublets, and dead

(19) Enhancing NK cell-mediated tumor killing of B7-H6+ cells with bispecific antibodies targeting allosteric sites of NKp30Molecular Therapy: OncologyMarch 20, 2025Léxane Fournier, Paul Arras, Lukas Pekar, Harald Kolmar, Stefan Zielonka, Lars Toleikis, Stefan Becker37°C. Functional binding of the expressed antibodies on NKp30-expressing cells was evaluated using an iQue3 (Sartorius). As an initial binding test, 105 NK-92 cells/well were seeded, and after two washing steps

(20) NI-3201 Is a Bispecific Antibody Mediating PD-L1-Dependent CD28 Co-stimulation on T Cells for Enhanced Tumor ControlCancer immunology researchMarch 4, 2025Majocchi S, Lloveras P, Nouveau L, Legrand M, Viandier A, Malinge P, Charreton M, Raymond C, Pace EA, Millard BL, et al.Cat. #A-865, RRID: AB_2536211, 1:500 diluted) was used for detection. Analysis was performed on an iQue3 screener equipment (Sartorius). Positive clones were reformatted from scFvs into human IgG1 using standard

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High-Throughput Screening by Cytometry

iQue® 3 High-Throughput Screening Cytometry Platform from Sartorius

Description

Specifications/Features

Sartorius

Sartorius

Citations (62)

Reviews

Bench Tips

How to Streamline iPSC Research and Development Workflows

Streamline Cell-Line Development with Advanced Flow Cytometry

Enhance Your Workflow with Advanced Flow Cytometry

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Streamlining Research Workflows with Advanced Flow Cytometry

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iQue® 3 High-Throughput Screening Cytometry Platform from Sartorius

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