Fixation Buffer from BioLegend

Product Specs
ItemFixation Buffer
CompanyBioLegend
Price $59.00Supplier Page View Company Product Page
Catalog Number420801
ApplicationsICFC - Quality tested, ICC
Quantity100 mL

Add to Compare List

BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Evaluation of Safety and Immunogenicity of High-Dose Quadrivalent Seasonal Influenza Split Vaccine: A Preclinical StudyVaccines (Basel)May 17, 2026Lanxin Jia, Ran Qiu, Jing Liu, Bo Liu, Xuanxuan Nian, Yang Le, Xixin Han, Qingmei Zhang, Xuedan Li, Zheng Gong, et al.min). Each sample tube received 500 μL of Fixation Buffer (BioLegend, San Diego, CA, USA; Cat. No. 420801) to fix the cells, which were incubated at room temperature in the dark for 20 min. After fixation,

(2) Activation-gated, T cell-restricted silencing of Dapk1 enhances Bacille Calmette-Guérin-elicited protective CD4+ memoryiScienceMay 15, 2026Zixuan Liu, Weihuang Liu, Chenyu Zhao, Xuanchang Bai, Fangting Zhou, Hang Sun, Jiahe Shi, Pinru Chen, Min Liu, Qin Pansubstrate,Biosharp,Cat# BL523B Zombie NIRTM Fixable Viability Kit,Biolegend,Cat# 423105 Fixation Buffer,Biolegend,Cat# 420801 Intracellular Staining Perm Wash Buffer(10×),Biolegend,Cat# 421002 Brefeldin A,Absin,Cat# abs810012 Cell lysis buffer

(3) NDRG3 is essential for sustaining antigen-driven T cell responses by protecting against restimulation-induced cell deathJ ImmunolMay 14, 2026Brian J Stack, Susan M KaechFoxp3/Transcription Factor Staining Buffer Set,00-5523-00,Thermo Fisher Scientific Fixation Buffer,420801,BioLegend Lonza P3 kit,V4XP-3032,Lonza S.p. Cas9 Nuclease V3,1081059,Integrated DNA Technologies "Goat

(4) Endogenous CD28 drives the persistent activity of CAR T cells in myeloma and lymphoma modelsBlood Cancer DiscovMay 5, 2026Mackenzie M. Lieberman, Jason H. Tong, Nkechi U. Odukwe, Colin A. Chavel, Gina G. Bishara, Kimberly M. Crasti, Megan M. Herr, Payal Goala, Terence J. Purdon, Rebecca Burchett, et al.following fixation and permeabilization according to manufacturer’s instructions (BioLegend, catalog #420801 & #421002). Antibodies used in phenotypic analysis are included in the Key Resources Table and gating

(5) A mouse model to study persistent fatigueResearch SquareMay 4, 2026Adam J. Janowski, Kazuhiro Hayashi, Ashley N. Plumb, Joseph B. Lesnak, Angela F. Smith, Lynn Rasmussen, Elizabeth Gross, Andrew A. Post, Giovanni Berardi, Marguerita E. Klein, et al.flow cytometry staining buffer and fixed using a 4% paraformaldehyde solution (BioLegend Fixation Buffer, Cat. #420801; San Diego, CA, USA). Samples underwent a final wash and were resuspended in flow cytometry staining

(6) Single-cell analysis highlights the significance of malignant cell IFN/MHC-II for immunotherapy response in head and neck squamous cell carcinomaCell Reports MedicineApril 21, 2026Michael Mints, Reilly A. Sample, Anuraag S. Parikh, Jesse M. Zaretsky, Zongtai Qi, Travis Law, Fudong Wang, Thomas F. Barrett, Riley Mullins, Ashley Reeb, et al.of phospho-STAT1, live cells were fixed at 37°C for 15 min in pre-warmed Fixation Buffer (BioLegend, 420801). Fixed cells were washed 3 times in PBS +0.5% BSA and subsequently permeabilized in pre-chilled True-Phos Perm Buffer

(7) Novel NK cell-like phenotype expressing CCR5 and its ligands elicits tumor-specific acquired immunity to carcinomatous peritonitis via host NK-derived IFN-γJ Immunother CancerApril 20, 2026Situo Zheng, Yui Harada, Yosuke Morodomi, Noriko Yasuda, Kenta Ishimoto, Yoshikazu YonemitsuUSA). After 6 hours, following surface staining, the cells were fixed with fixation buffer (BioLegend, 420801, California, USA) for 20 min at room temperature, then washed two times using 1x Intracellular Staining

(8) Sunitinib induces immunogenic cell death through eIF2α phosphorylation to potentiate immunotherapy in HCCiScienceApril 17, 2026Yimei Gu, Hanbing Mai, Peijian Huang, Hui Yuan, Mingxuan Sun, Huixin Gao, Chenyu Shang, Yangfen Ou, Jizhou Tan, Xianzhang Huang, et al.incubation, cells were washed for intracellular staining. Cells were then fixed with Fixation Buffer (Cat#420801, BioLegend) and permeabilized with Permeabilization Buffer (Cat#00-8333-56, Invitrogen). Cells were

(9) Enhanced Th1 Cellular Immunity Induced by an RSV-F mRNA Vaccine Rationally Designed Using NLP AlgorithmsVaccines (Basel)April 16, 2026Zhi-Wu Xia, Qi Tang, Jun-Jie Pan, Jing Liu, Lan-Xin Jia, Guo-Mei Zhang, Man-Ni Xie, Jia-Hao Zheng, Chuan-Shuo Lv, Lei Zhang, et al.CD8 (Biolegend, Cat. #100706) for 15–30 min at RT. After fixation (Biolegend Fixation Buffer, Cat. #420801) and permeabilization (Biolegend Intracellular Staining Permeabilization Wash Buffer, Cat. #421002),

(10) Payload Diversification Overcomes Resistance and Guides Sequential Antibody-Drug Conjugate Therapy in Breast CancerClin Cancer ResApril 15, 2026Dileep Reddy Rampa, Minji Seo, Nanae Ogata, Zhaoxuan Yang, Nithya Sridhar, Takeo Fujii, Chalothorn Wannaphut, Jennifer A. Maynard, Kyoji Tsuchikama, George W. Sledge, et al.then replaced with antibody-free culture medium, cells were fixed with fixation buffer (BioLegend, 420801), mounted with DAPI-containing medium (ibidi, 50011), and 0 h images were acquired. A parallel plate

(11) Phenanthrene Exposure Induces Immune Dysfunction and Metabolic Disruption in MiceACS OmegaApril 15, 2026Qiaojuan Liu, Yan Zhang, Fenfang Deng, Wenxue Li, Guangyu Yang, Jun Yuan, Yuehong Wei, Juntao Li, Lei Tanand permeabilized using the Intracellular Fixation/Permeabilization Buffer Set (BioLegend, Cat. No. 420801/421002), followed by staining with fluorochrome-labeled antibodies against IFN-γ and TNF-α. All stained

(12) Payload Diversification Overcomes Resistance and Guides Sequential Antibody–Drug Conjugate Therapy in Breast CancerClinical Cancer ResearchApril 15, 2026Dileep Reddy Rampa, Minji Seo, Nanae Ogata, Zhaoxuan Yang, Nithya Sridhar, Takeo Fujii, Chalothorn Wannaphut, Jennifer A. Maynard, Kyoji Tsuchikama, George W. Sledge, et al.then replaced with antibody-free culture medium, cells were fixed with fixation buffer (BioLegend, 420801), mounted with DAPI-containing medium (ibidi, 50011), and 0 h images were acquired. A parallel plate

(13) Naturally self-reactive B cells are poised to cross the selection barrier into autoimmune germinal centersCell ReportsApril 8, 2026Danni Yi-Dan Yi-Dan Zhu, Maya Sangesland, Vintus Okonkwo, Zhenrui Zhang, Victoria Rosado, Larance Ronsard, Caitlin McCarthy, Caroline Alexander, Ralston M. Barnes, Daniel Rohrer, et al.dye Fixable live/dead stain Efluor 780,eBioscience,cat# 65–0865-18 Fixation buffer,Biolegend,cat# 420801 HBSS (Hanks Balanced Salt Solution),Corning,cat# 21–022-CV "HEPES, liquid 1M solution",Corning,cat#

(14) A 3D microfluidic model of exchange between perfused blood and lymphatic microvascular networksLab on a ChipApril 7, 2026Delaney Gray-Scherr, Terry Ching, Katherine Beran, Sarah C. Adams, Emily Davis, Abraham C. I. van Steen, Trisha Raman, Wilson W. Wong, Jeroen Eyckmans, Christopher S. Chenimaging studies. Immunostaining and imaging Devices were fixed with 4% paraformaldehyde (BioLegend, 420801) in PBS for 15 minutes, followed by PBS wash and permeabilization with 0.1% Triton X-100 (Sigma, T8787)

(15) EBV Triggers a Distinct Antiviral Response in HMC3 CellsbioRxivApril 7, 2026Noah E. Berkowitz, Alexander Nosov, Mark Nosov, Fryda Solis Roldan, Anuj Ahuja, Miranda McGaskey, Ethel Cesarman, Douglas F. Nixon, Nicholas Dopkinsroom temperature conditions using a fixation buffer containing 4% paraformaldehyde (BioLegend, Cat# 420801). Fixation buffer was then decanted and 200μL of DAPI Fluoromount-G® (SouthernBiotech, Cat# 0100–20)

(16) A periplasmic protein complex mediates arabinofuranosyltransferase activity and intrinsic drug resistance in Mycobacterium tuberculosisSci AdvApril 3, 2026Thaís Klevorn, Christopher Brown, Christine D. Hardy, Bonnie J. Cuthbert, Amanda Spencer, Adrián Jinich, Luming Chan, Shiva K. Angala, Jordan Manzer, Jessica Mendoza, et al.supernatant containing DMN-Tre was discarded. Samples were fixed with 4% paraformaldehyde (PFA; BioLegend, #420801) for 4 hours at room temperature. After two washes in PBS with 0.05% Tween 80, bacteria were resuspended

(17) Krüppel-like factors 2 and 3 regulate T cell exhaustion by directing T cell residency and migrationImmunityMarch 27, 2026Jian Shen, Ryan J. Brown, Yuqi Zhang, Kexin Gai, Ashley K. Brown, Ashley M. Bauer, Ashley Zhu, Siying Lin, Hongshen Niu, Arjun Kharel, et al.19853 P3 Primary Cell 96-well Nucleofector Kit,Lonza,Cat# V4SP-3096 Fixation Buffer,BioLegend,Cat# 420801 True-Nuclear Transcription Factor Buffer Set,BioLegend,Cat# 424401 Foxp3 / Transcription Factor Staining

(18) Recombinant human adenovirus type 5 synergizes with anti-PD-L1 antibody to promote anti-hepatocellular carcinoma effects through multilevel remodeling of the immune microenvironmentFrontiers in ImmunologyMarch 24, 2026Qiu Zhao, Min Xiao, Jian Ma, Xiaoqian Li, Cong Fu, Wenjing Ji, Lingna Ni, Jialin Fan, Qianqian Gao, Yanzhi BiAPC-CD86 (BioLegend, 105011), Alexa Fluor® 700-CD206 (BioLegend, 141733), fixation buffer (BioLegend, 420801), and intracellular staining permeabilization wash buffer (BioLegend, 421002). 2.7 HE, immunohistochemistry The

(19) Peripheral immune landscape in pancreatic ductal adenocarcinoma reveals expansion of effector states with disease progressioniScienceMarch 20, 2026Chen-Hsuan Yang, Yun-Yi Lung, Chieh-Chang Chen, I-Hsuan Lo, Chia-Jung Lien, Wei-Kai Wu, Ming-Shiang Wu, Wei-Chih Liao, Jian-Da Linstain,NanoEnTek,Cat# EBT-001 Brilliant Stain Buffer,BD Biosciences,Cat# 566385 Fixation Buffer,Biolegend,Cat# 420801 ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, No critical

(20) Strain-level antigen variation facilitates immune evasion in Bacteroides thetaiotaomicronJ ImmunolMarch 18, 2026Robert W P Glowacki, Jessica M Till, Orion D Brock, Vladimir Makarov, Morgan J Engelhart, Philip P Ahernby pipetting in 100 µl of a commercially available formaldehyde-based fixative (BioLegend, Cat. no. 420801; used when no intracellular staining was subsequently performed) or (ThermoFisher, Cat. # 00-5123-43

Be the First to Write a Review!

Fixation Buffer from BioLegend

Description

BioLegend

Citations (547)

Reviews

Fixation Buffer from BioLegend

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map