Mouse Embryonic Fibroblast (MEF) Conditioned Media from R&D Systems, a Bio-Techne Brand

Product Specs
ItemMouse Embryonic Fibroblast (MEF) Conditioned Media
CompanyR&D Systems, a Bio-Techne Brand
Price $222.00Supplier Page View Company Product Page
Catalog NumberAR005
Quantity100 mL
TypeMedia
SpeciesMouse
StorageStore the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Modelling inflammation-induced peripheral sensitization in a dish—more complex than expected?PainFebruary 25, 2025Yuening Li, Amy Lock, Laura Fedele, Irene Zebochin, Alba Sabate, Matthew Siddle, Silvia Cainarca, Pascal Röderer, Katharina Montag, Paola Tarroni, et al.medium was changed to mouse embryonic fibroblast conditioned medium (MEF, Bio-Techne, United Kingdom, #AR005) supplemented with 10 ng/mL human recombinant FGF-2 (Miltenyi, #130-093-839). 24 hours later (day [D]

(2) Induction of Meiotic Initiation in Long-Term Mouse Spermatogonial Stem Cells Under Retinoid Acid and Nutrient Restriction ConditionsMethods in Molecular BiologyJune 27, 2024Xiaoyu Zhang, Ning Wangshould be used for the initial culture. Commercially available MEF cells, e.g., R&D system Catalog No. AR005, are used. According to the manufacturer’s website, MEF cells are collected from outbred CF1 mice, mitotically

(3) Quiescence enables unrestricted cell fate in naive embryonic stem cellsNature communicationsFebruary 26, 2024Khoa LTP, Yang W, Shan M, Zhang L, Mao F, Zhou B, Li Q, Malcore R, Harris C, Zhao L, et al.

(4) Naïve-like pluripotency to pave the way for saving the northern white rhinoceros from extinctionScientific reportsMarch 8, 2022Zywitza V, Rusha E, Shaposhnikov D, Ruiz-Orera J, Telugu N, Rishko V, Hayashi M, Michel G, Wittler L, Stejskal J, et al.on one well of a Geltrex (Life Technologies, #A1413202) coated 6-well plate in mTeSR1 (R&D System, #AR005) for 3–4 days until the cells were 90–100% confluent. Thereafter, the medium was changed to RPMI medium

(5) Synapse alterations precede neuronal damage and storage pathology in a human cerebral organoid model of CLN3-juvenile neuronal ceroid lipofuscinosisActa neuropathologica communicationsDecember 30, 2019Gomez-Giro G, Arias-Fuenzalida J, Jarazo J, Zeuschner D, Ali M, Possemis N, Bolognin S, Halder R, Jäger C, Kuper WFE, et al.minus three). Media was exchanged at day minus two for MEF conditioned media (R&D Systems, cat no. AR005) supplemented with 10 ng/mL bFGF (PreproTech, cat no. 100-18B). At day minus one, media was refreshed

(6) Trisomy silencing by XIST normalizes Down syndrome cell pathogenesis demonstrated for hematopoietic defects in vitroNature communicationsDecember 5, 2018Chiang JC, Jiang J, Newburger PE, Lawrence JB.

(7) Embryos and embryonic stem cells from the white rhinocerosNature communicationsJuly 4, 2018Hildebrandt TB, Hermes R, Colleoni S, Diecke S, Holtze S, Renfree MB, Stejskal J, Hayashi K, Drukker M, Loi P, et al.(#S1049, SelleckChem) on one well of a Geltrex-coated 6-well plate in MEF conditioned media (R&D System AR005) with 10 ng/ml FGF 2 for 3–4 days until the cells were 90–100% confluent. Thereafter the GSK 3 inhibitor

(8) Multicellular tumor spheroids of human uveal melanoma induce genes associated with anoikis resistance, lipogenesis, and SSXsMolecular visionJanuary 1, 2017Ness C, Garred Ø, Eide NA, Kumar T, Olstad OK, Bærland TP, Petrovski G, Moe MC, Noer A.

(9) Modulating innate immunity improves hepatitis C virus infection and replication in stem cell-derived hepatocytesStem cell reportsJuly 8, 2014Zhou X, Sun P, Lucendo-Villarin B, Angus AG, Szkolnicka D, Cameron K, Farnworth SL, Patel AH, Hay DC.

(10) Mitf is the key molecular switch between mouse or human melanoma initiating cells and their differentiated progenyOncogeneMay 19, 2011Cheli Y, Giuliano S, Botton T, Rocchi S, Hofman V, Hofman P, Bahadoran P, Bertolotto C, Ballotti R.

(11) Mitochondrial function controls proliferation and early differentiation potential of embryonic stem cellsStem CellsMarch 1, 2011Mandal S, Lindgren AG, Srivastava AS, Clark AT, Banerjee U.

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