Description
- Isolated from a recombinant source
- Supplied with 10X Reaction Buffer
- Specific for single-stranded DNA or RNA
Description:
Exonuclease T (Exo T), also known as RNase T, is a single-stranded DNA or RNA specific nuclease that requires a free 3' terminus and removes nucleotides in the 3' → 5' direction. Exonuclease T can be used to generate blunt ends from a DNA molecule that has a 3' extension. The efficiency of this reaction is sequence dependent and may result in a mixture of products having 1 or 2 base 3' recessed ends as well as blunt ends.
Source:
Exonuclease T is overexpressed and purified as a C-terminal fusion to maltose-binding protein (MBP). MBP is removed from Exonuclease T by Factor Xa cleavage and Exonuclease T is then purified away from Factor Xa and MBP. Exonuclease T cleaved from MBP has an additional amino acid on the N-terminus and a Phe instead of a Met (i.e., Glu-Phe-Exo T instead of Met-Exo T).
Reagents Supplied:
NEBuffer 4 (10X)