Odyssey® XF Infrared Imaging System from LICORbio

Product Specs
ItemOdyssey® XF Infrared Imaging System
CompanyLICORbio
Price Get Pricing Get Quote for this Product
Catalog NumberOdyssey XF
QuantityEA
ApplicationsNear-Infrared Fluorescent Western Blot, Chemiluminescent Western Blot, Protein Gel Documentation, Nucleic Acid Gel Documentation
Resolution125 µm
Light Source525 nm LED
SoftwareLI-COR® Acquisition Software and Empiria Studio® Software
Wavelength(s)Solid-state diode laser at 685 nm, Solid-state diode laser at 785 nm
Imaging Size(WxD) 12 x 10 cm (4.7 x 3.9 in.)
Range(Dynamic) >6 logs

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LICORbio

4647 Superior

St. Lincoln, NE 68504

United States

Phone: 800-645-4267
402-467-0700

Company Profile

Website: Website: www.licor.com/bio

Website: Reagents: www.licor.com/bio/reagents

Website: Special offers: www.licor.com/bio/special-offers

(1) Endoxifen downregulates AKT phosphorylation through protein kinase C beta 1 inhibition in ERα+ breast cancerNPJ Breast CancerDecember 19, 2023Swaathi Jayaraman, Xinyan Wu, Krishna R. Kalari, Xiaojia Tang, Mary J. Kuffel, Elizabeth S. Bruinsma, Shahrzad Jalali, Kevin L. Peterson, Cristina Correia, Rachel A. Kudgus, et al.West Pico (Thermo Scientific #34580) or West Femto (Thermo Scientific #34096) reagents and a Li-Cor Odyssey® XF imager. Protein lysates from the MCF7AC1 xenograft model were obtained from a previous study11. Quantitation

(2) Transcriptome analysis reveals molecular signature and cell-type difference of Homo sapiens endothelial-to-mesenchymal transitionG3: Genes, Genomes, GeneticsDecember 6, 2023Ronald Bronson, Junfang Lyu, Jianhua Xiongor SuperSignal West Pico PLUS Chemiluminescent Substrate (34,579, Thermo Fisher Scientific) under an Odyssey XF Imager (LI-COR Biosciences) and analyzed using Empiria Studio software (LI-COR Biosciences). The following

(3) Senescence-associated secretory phenotypes in mesenchymal cells contribute to cytotoxic immune response in oral lichen planusImmun AgeingDecember 5, 2023Shogo Ijima, Yuki Saito, Sena Yamamoto, Kentaro Nagaoka, Taiki Iwamoto, Arisa Kita, Maki Miyajima, Tsukasa Sato, Akihiro Miyazaki, Takako S. Chikenji680RD, 1:20000, LI-COR) secondary antibodies. Fluorescence-based detection was performed using LI-COR Odyssey XF (LI-COR). SA-β-Gal staining To detect cellular senescence, we performed SA-β-Gal staining using the

(4) Cyclin D1 extensively reprograms metabolism to support biosynthetic pathways in hepatocytesJournal of Biological ChemistryDecember 1, 2023Heng Wu, Betsy T. Kren, Andrew N. Lane, Teresa A. Cassel, Richard M. Higashi, Teresa W.M. Fan, George S. Scaria, Laurie L. Shekels, Mark A. Klein, Jeffrey H. Albrechtrecommended by the manufacturer. Western blot images and quantification were performed using the Licor Odyssey XF Imaging System. Quantified protein expression was normalized to actin expression in each specimen. For reverse phase

(5) A CRISPR-drug perturbational map for identifying compounds to combine with commonly used chemotherapeuticsNature CommunicationsNovember 13, 2023Hyeong-Min Lee, William C. Wright, Min Pan, Jonathan Low, Duane Currier, Jie Fang, Shivendra Singh, Stephanie Nance, Ian Delahunty, Yuna Kim, et al.HRP-conjugated anti-rat at RT for 30 min. After rinsing three times, the membrane was imaged by Licor Odyssey XF to measure DNA adduct (Supplementary Data Fig. 11). Genetic validation of sensitized candidates Three

(6) Low Glycolysis Is Neuroprotective during Anoxic Spreading Depolarization (SD) and Reoxygenation in LocustseNeuroNovember 6, 2023Yuyang Wang (王宇扬), Alexander G. Little, Maria J. Aristizabal, R. Meldrum Robertson(LI-COR), before acquiring the signal for anti-dinitrophenyl (DNP; supplied in ab178020, 1:5000). An Odyssey XF imaging system was used to image the membrane; the whole-lane signals were quantified and then normalized against total

(7) Cytokine polarized, alternatively activated bone marrow neutrophils drive axon regenerationResearch SquareNovember 1, 2023Andrew D. Jerome, Andrew R. Jerome, Andrew Wang, Jing Wang, Jeffrey R. Atkinson, Jeffrey Atkinson, Amy Webb, Benjamin M. Segal1:2500). Blots were visualized using SuperSignal West Femto (ThermoFischer) reagent and imaged on an Odyssey XF Imager (LiCor). Blot analysis was performed using Image Lab (Bio-Rad). Immunohistochemistry and quantification

(8) CXCL17 binds efficaciously to glycosaminoglycans with the potential to modulate chemokine signalingFrontiers in ImmunologyOctober 24, 2023Sean P. Giblin, Sashini Ranawana, Shyreen Hassibi, Holly L. Birchenough, Kyle T. Mincham, Robert J. Snelgrove, Tomoko Tsuchiya, Shiro Kanegasaki, Douglas Dyer, James E. Peasesubstrate and was imaged using an iBright™ instrument (ThermoFisher). Fluorescence was detected using an Odyssey XF imager (LI-COR, Cambridge UK). 2.11 Glycosaminoglycan solid-phase binding assays Heparin from porcine

(9) Canagliflozin and irbesartan ameliorate renal fibrosis via the TGF-β1/Smad signaling pathway in Dahl salt-sensitive ratsJournal of International Medical ResearchOctober 20, 2023Jianlong Zhai, Zhongli Wang, Tingting Zhang, Lili He, Sai Ma, Qingjuan Zuo, Guorui Zhang, Xinyu Wang, Yifang Guoenhanced chemiluminescence (Thermo Fisher Scientific), the antigen–antibody complexes were detected using Odyssey XF (LI-COR, Lincoln, NE, USA). The signal intensities of target bands were analyzed using ImageJ 8.0. The

(10) Multi-Omics Profiling of Human Endothelial Cells from the Coronary Artery and Internal Thoracic Artery Reveals Molecular but Not Functional HeterogeneityInternational Journal of Molecular SciencesOctober 9, 2023Alexey Frolov, Arseniy Lobov, Marsel Kabilov, Bozhana Zainullina, Alexey Tupikin, Daria Shishkova, Victoria Markova, Anna Sinitskaya, Evgeny Grigoriev, Yulia Markova, et al.a 1:1000 dilution. Incubation with the antibodies and fluorescent detection was performed using an Odyssey XF imaging system (LI-COR Biosciences, Lincoln, NE, USA) at a 700 nm channel (685 nm excitation and 730 nm emission).

(11) Disruption of Epithelium Integrity by Inflammation-Associated Fibroblasts through Prostaglandin Signaling: IAFs disrupt colon epithelium via PGE2-EP4bioRxivOctober 5, 2023Yi Dong, Blake A. Johnson, Linhao Ruan, Maged Zeineldin, Albert Z. Liu, Sumana Raychaudhuri, Ian Chiu, Jin Zhu, Barbara Smith, Nan Zhao, et al.settings. For imaging, ECL substrate (BioRad, Cat# 170–5060) was used as the detection reagent and the Odyssey XF imaging system (LI-COR Biosciences) was used to record images. Restore Plus Western blot stripping buffer (ThermoFisher

(12) Mutations Causing X-Linked Amelogenesis Imperfecta Alter miRNA Formation from Amelogenin Exon4Journal of Dental ResearchOctober 1, 2023R. Shemirani, M.H. Le, Y. NakanoFisher Scientific) and the 5% Mini-PROTEAN TBE Gel (Bio-Rad). Bands were scanned and visualized with the Odyssey XF scanner (Licor) and Empiria Software (Licor). Electrophoretic Mobility Shift Assay Cy5.5-labeled RNA

(13) Biochemical evidence that the whole compartment activity behavior of GAPDH differs between the cytoplasm and nucleusPLOS ONEAugust 31, 2023Helen S. Tang, Chelsea R. Gates, Michael C. Schultzby exposure to film (GE Healthcare, Amersham HyperfilmTM ELC) or by use of a LI-COR digital imager (Odyssey® XF Imaging System). Quantitative analysis was performed according to the updated method outlined above, except that the

(14) Small Heterodimer Partner Modulates Macrophage Differentiation during Innate Immune Response through the Regulation of Peroxisome Proliferator Activated Receptor Gamma, Mitogen-Activated Protein Kinase, and Nuclear Factor Kappa B PathwaysBiomedicinesAugust 28, 2023Forkan Ahamed, Natalie Eppler, Elizabeth Jones, Lily He, Yuxia ZhangSuperSignal West Femto Chemiluminescent Substrate (Fisher, PI34094). Images were captured using an Odyssey XF LI-COR imaging system (Lincoln, NE, USA). To ensure equal protein loading, loading controls such as

(15) Dickkopf1 induces enteric neurogenesis and gliogenesis in vitro if apoptosis is evadedCommun BiolAugust 2, 2023Melanie Scharr, Simon Scherer, Bernhard Hirt, Peter H. NeckelFast Western-Blot Kit and the SuperSignal West Femto Substrate. The blots were scanned with the LICOR Odyssey XF Imaging System with an exposure time of 10 minutes each. The optical density was analysed using ImageJ (Supplementary

(16) A conserved STRIPAK complex is required for autophagy in muscle tissueMolecular Biology of the CellAugust 1, 2023Yungui Guo, Qiling Zeng, David Brooks, Erika R. GeisbrechtBiosciences, Lincoln, NE) was used as a loading control. Membranes were developed using the LI-COR Odyssey XF and quantitation of relative protein levels was performed in Empiria Studio Software (LI-COR Biosciences,

(17) Structure and function of a dual antagonist of the human growth hormone and prolactin receptors with site-specific PEG conjugatesThe Journal of biological chemistryAugust 1, 2023Basu R, Brody R, Sandbhor U, Kulkarni P, Davis E, Swegan D, Caggiano LJ, Brenya E, Neggers S, Kopchick JJ.Chemiluminescence detection reagents (Thermo Fisher Scientific). Chemiluminescence signals were detected using an Odyssey XF (LI-COR) imaging system. Densitometry analyses of the blots were performed using ImageJ Software (https://imagej.nih.gov/ij/)

(18) Disease modeling and pharmacological rescue of autosomal dominant Retinitis Pigmentosa associated with RHO copy number variationmedRxivJuly 19, 2023Sangeetha Kandoi, Cassandra Martinez, Kevin Xu Chen, Miika Mehine, Brian C. Mansfield, Jacque L. Duncan, Deepak A. Lambawere washed another three times with TBS-T buffer (10 mins interval each) and visualized using LiCor Odyssey XF scanner. For quantification of protein expression, the background was subtracted for each band and normalized

(19) IKK2/NFkB signaling controls lung resident CD8+ T cell memory during influenza infectionNature CommunicationsJuly 19, 2023Curtis J. Pritzl, Dezzarae Luera, Karin M. Knudson, Michael J. Quaney, Michael J. Calcutt, Mark A. Daniels, Emma TeixeiroHercules, CA) and probed with specific primary and secondary antibodies. Blots were imaged on a Li-Cor Odyssey XF (Li-Cor, Lincoln, NE) and analyzed using Image Studio v5.2.5 (Li-Cor, Lincoln, NE). A table of antibodies

(20) Regulation of Rad52-dependent replication fork recovery through serine ADP-ribosylation of PolD3Nature communicationsJuly 18, 2023Richards F, Llorca-Cardenosa MJ, Langton J, Buch-Larsen SC, Shamkhi NF, Sharma AB, Nielsen ML, Lakin ND.Immobilon Western Chemiluminescent HRP substrate (Millipore) was detected using autoradiography or the Odyssey® XF Imaging system (LI-COR Biosciences). LI-COR images were quantified with Image StudioTM. Antibodies are listed in Supplementary

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Odyssey® XF Infrared Imaging System from LICORbio

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Odyssey® XF Infrared Imaging System from LICORbio

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