PathScan ® Sandwich ELISA Lysis Buffer (1X) from Cell Signaling Technology

Product Specs
ItemPathScan ® Sandwich ELISA Lysis Buffer (1X)
CompanyCell Signaling Technology
Price $55.00 Inquire
Catalog Number7018S
Quantity30 ml
ApplicationsELISA
Research AreaRelated Products
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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

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Website: http://www.cellsignal.com/

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(1) Development of the First Small-Molecule Inhibitor Targeting Oncostatin M for Treatment of Breast CancerJ Med ChemAugust 14, 2025Cody L. Wolf, Andrea Feci, Joseph P. Tuccinardi, Grace H. Coughlin, Kelsey A. Holdaway, Thaaer Muhammed, Clyde Pruett, Darren Lighter, Cooper McGrath, Terrell Engmann, et al.afterward, the cells were lysed using 1x PathScan Sandwich ELISA Lysis Buffer (Cell Signaling Technology; #7018S), collected, and stored at −20 °C. Lysates were analyzed for pSTAT3 levels using either the PathScan Phospho-STAT3 (Tyr705) Sandwich ELISA Antibody Pair

(2) αB-crystallin enhances radioresistance in basal-like breast cancer cells by suppressing caspase-3 activationSci ProgMay 20, 2025Yan Yuan, Xubaihe Zhang, Ying Xu, Yangyang Ge, Anqing Wuradiation exposure, the cells were harvested and lysed on ice for 5 minutes using a lysis buffer (Cat. No: 7018S; Cell Signaling Technology, USA). The lysates were then centrifuged at 10,000 × g for 10 minutes at

(3) Combination of RNA-sequencing data analysis, network pharmacology, molecular docking techniques to investigate the mechanism of Prunella vulgaris L. in the treatment of non-small cell lung cancerDiscov OncolMay 12, 2025Yanggang Dan, Yangli Jin, Jing Wang, Lu Wang, Dawei Zhengconcentrations of morin (50 μM or 100 μM) for 24 h. The cells were harvested and lyzed with 50 µL lysis buffer (#7018; Cell Signaling Technology, Shanghai, China). Subsequently, after centrifugation at 14,000×g for 10

(4) Chemokine-derived oncolytic peptide induces immunogenic cancer cell death and significantly suppresses tumor growthCell Death DiscoveryApril 2, 2024Natsuki Furukawa, Wendy Yang, Alex R. Chao, Akash Patil, Adam C. Mirando, Niranjan B. Pandey, Aleksander S. Popelovernight. After CKS1 treatment, the cells were lysed using a lysis buffer (Cell Signaling Technology, #7018). The activity of caspase-3 in the cell lysate was quantified using the caspase-3 activity assay kit

(5) Pan-cancer analysis of NUDT21 and its effect on the proliferation of human head and neck squamous cell carcinomaAging (Albany NY)February 12, 2024Wenjing Liu, Yingna Pang, Xiaolu Yu, Doudou Lu, Yating Yang, Fandi Meng, Chengbi Xu, Ling Yuan, Yi Nanintracellular signaling. PathScan® Intracellular Signaling Array Kit (Cell Signaling Technology, USA, #7018) was used to detect changes in signaling molecules. All experiments followed the experimental process

(6) The Rac inhibitor HV-107 as a potential therapeutic for metastatic breast cancerMolecular MedicineJune 14, 2023Grace Velez Crespo, Jescelica Ortiz, Eliud Hernández O’Farrill, Cornelis P. Vlaar, Mikhail Inyushin, Yuriy Kucheryavykh, Lilia KucheryavykhLincoln, NE, USA). Western blot analysis Cells were lysed in 1X PathScan Sandwich ELISA lysis buffer (#7018, Cell Signalling, Denver, MA, USA) supplemented with a 1 mM protease inhibitor cocktail. Clarified cell

(7) Tau Regulates Glioblastoma Progression, 3D Cell Organization, Growth and Migration via the PI3K-AKT AxisCancersNovember 19, 2021Pagano A, Breuzard G, Parat F, Tchoghandjian A, Figarella-Branger D, De Bessa TC, Garrouste F, Douence A, Barbier P, Kovacic H.least three times. 2.7. Western Blot Analysis Cells or MCS were lysed in Pathscan lysis buffer (#7018, Cell Signaling Technology, Danvers, MA, USA): 20 mM Tris-HCl pH 7.5, 150 mM NaCl, 1 mM disodium EDTA,

(8) Bioactive recombinant human oncostatin M for NMR-based screening in drug discoveryScientific reportsAugust 10, 2021Mass OA, Tuccinardi J, Woodbury L, Wolf CL, Grantham B, Holdaway K, Pu X, King MD, Warner DL, Jorcyk CL, et al.cell lysates were collected with 1X Cell Lysis Buffer (Cell Signaling Technology, Danvers, MA; Cat# 7018). Lysates were run on an SDS-PAGE gel and transferred onto a nitrocellulose membrane via semi-dry transfer.

(9) Long-chain polyphosphates impair SARS-CoV-2 infection and replicationScience signalingJuly 6, 2021Ferrucci V, Kong DY, Asadzadeh F, Marrone L, Boccia A, Siciliano R, Criscuolo G, Anastasio C, Quarantelli F, Comegna M, et al.the manufacturer’s instructions. Briefly, the cells were lysed in 30 μl of PathScan lysis buffer (no. 7018, Cell Signaling Technology). Cell lysates (4 μg) were mixed with substrate solution (N-acetyl-Asp-Glu-Val-Asp-7-amino-4-methylcoumarin;

(10) Downregulation of long non‑coding RNA GACAT1 suppresses proliferation and induces apoptosis of NSCLC cells by sponging microRNA‑422aInternational Journal of Molecular MedicineDecember 22, 2020Youqing Zhong, Hui Lin, Qi Li, Chang Liu, Lei ZhongsiRNA-GACAT1. After transfection for 48 h, cells were harvested and lysed with 50 µl lysis buffer (cat. no. 7018; Cell Signaling Technology, Inc.). A total of 25 µl lysate solution was mixed with 200 µl substrate

(11) Insight into the Interactome of Intramitochondrial PKA Using Biotinylation-Proximity LabelingInternational journal of molecular sciencesNovember 5, 2020Ould Amer Y, Hebert-Chatelain E.the indicated plasmids were resuspended in lysis buffer (PathScan® Sandwich ELISA 1X, Cell Signaling #7018) supplemented with 1% protease inhibitor cocktail, 2 mM sodium orthovanadate and 1 mM sodium fluoride.

(12) Polarized human cholangiocytes release distinct populations of apical and basolateral small extracellular vesiclesMol Biol CellOctober 15, 2020Brian A. Davies, Leslie O. Morton, John R. Jefferson, Cody N. Rozeveld, Luke C. Doskey, Nicholas F. LaRusso, David J. Katzmannwere washed in cold PBS and lysed in PathScan Sandwich ELISA Lysis Buffer (Cell Signaling Technologies 7018) with the addition of Complete EDTA-free Protease Inhibitor Cocktail Tablet (Roche 04693132001), PhosSTOP

(13) Polarized human cholangiocytes release distinct populations of apical and basolateral small extracellular vesiclesMolecular Biology of the CellOctober 15, 2020Brian A. Davies, Leslie O. Morton, John R. Jefferson, Cody N. Rozeveld, Luke C. Doskey, Nicholas F. LaRusso, David J. Katzmannwere washed in cold PBS and lysed in PathScan Sandwich ELISA Lysis Buffer (Cell Signaling Technologies 7018) with the addition of Complete EDTA-free Protease Inhibitor Cocktail Tablet (Roche 04693132001), PhosSTOP

(14) ABTB2 Regulatory Variant as Predictor of Epirubicin-Based Neoadjuvant Chemotherapy in Luminal A Breast CancerFrontiers in OncologySeptember 25, 2020Yajie Gong et al.were rinsed with ice-cold phosphate-buffered saline and subsequently cell lysis buffer was added (#7018, Cell Signaling Technology, Danvers, MA, USA). Caspase activity assay (Caspase-3 Activity Assay Kit

(15) Dendritic cell derived exosomes loaded with immunoregulatory cargo reprogram local immune responses and inhibit degenerative bone disease in vivoJournal of extracellular vesiclesAugust 7, 2020Elashiry M, Elashiry MM, Elsayed R, Rajendran M, Auersvald C, Zeitoun R, Rashid MH, Ara R, Meghil MM, Liu Y, et al.computationally predicted miRNA-mRNA target relationships [37]. ELISA EXO lysed with ELISA lysis buffer (#7018, Cell signaling) or intact were suspended in PBS and analysed according to the manufacturer, using ELISA

(16) Evolution of tumor cells during AsiDNA treatment results in energy exhaustion, decrease in responsiveness to signal, and higher sensitivity to the drugEvolutionary ApplicationsAugust 1, 2020Pierre‐Marie Girard, Nathalie Berthault, Maria Kozlac, Sofia Ferreira, Wael Jdey, Srividya Bhaskara, Sergey Alekseev, Frederic Thomas, Marie Dutreixcell scraper and boiled for 10 min in PathScan Sandwich ELISA Lysis Buffer (Cell Signaling Technology, 7018) and 1 mM PMSF (phenylmethanesulfonyl fluoride; Sigma, P7626) after treatment. Quantification of total

(17) Treatment with catalpol protects against cisplatin‑induced renal injury through Nrf2 and NF‑κB signaling pathwaysExperimental and Therapeutic MedicineJuly 29, 2020Jun Zhang, Li Liu, Furong Li, Zongqian Wang, Jinghong Zhaowere homogenized with 1 ml PBS (pH 7.4, 100 mM) and PathScan® Sandwich ELISA lysis buffer (cat. no. 7018; Cell Signaling Technology, Inc.) was used to lyse the tissues before centrifugation at 3,660 x g for

(18) Cordyceps cicadae Prevents Renal Tubular Epithelial Cell Apoptosis by Regulating the SIRT1/p53 Pathway in Hypertensive Renal InjuryEvidence-based complementary and alternative medicine : eCAMJanuary 1, 2020Huang YS, Wang X, Feng Z, Cui H, Zhu Z, Xia C, Han X, Liu WJ, Liu YN.Assay of Cells Cells were treated for 72 h and then lysed in PathScan Sandwich ELISA lysis buffer (1×; 7018, Cell Signaling Technology). Cell lysate was added to the assay plates containing the substrate solution,

(19) Knockdown Of CCDC132 Attenuates Gastric Cancer Cells Proliferation And Tumorigenesis By Facilitating DNA Damage SignalingCancer Manag ResNovember 12, 2019Xiaowu Xu, Weilang Cao, Wei Sun, Zhaohong Wang, Hui Chen, Zhiqiang Zheng, Xiaomin Yangextracts from the indicated cells were prepared by PathScan® Sandwich ELISA Lysis Buffer (cat. no. 7018; Cell Signaling, USA). Protein concentrations were determined using the BCA Protein Assay Kit (cat.

(20) Knockdown Of CCDC132 Attenuates Gastric Cancer Cells Proliferation And Tumorigenesis By Facilitating DNA Damage SignalingCancer Management and ResearchNovember 12, 2019Xiaowu Xu, Weilang Cao, Wei Sun, Zhaohong Wang, Hui Chen, Zhiqiang Zheng, Xiaomin Yang16-Nov-2021 For personal use only. Xu et al prepared by PathScan® Sandwich ELISA Lysis Buffer (cat. no. 7018; Cell Signaling, USA). Protein concentrations were determined using the BCA Protein Assay Kit (cat.

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PathScan ® Sandwich ELISA Lysis Buffer (1X) from Cell Signaling Technology

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PathScan ® Sandwich ELISA Lysis Buffer (1X) from Cell Signaling Technology

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