EcoRV-HF from New England Biolabs

Product Specs
ItemEcoRV-HF
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberR3195S
Quantity4000 units
Concentration20,000 units/ml
ApplicationsDNA Manipulation
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeRestriction Enzyme
Temperature-20 C

Sequence

GAT⇓ATC
CTA⇑TAG

Add to Compare List

New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Genome sequence and characterization of a novel Pseudomonas putida phage, MiCathSci RepDecember 9, 2023James Jaryenneh, Joseph S. Schoeniger, Catherine M. MageeneyR3101S), BamHI-HF (NEB, catalog no. R3136S), BstXI (NEB, catalog no. R0113S), EcoRV-HF (NEB, catalog no. R3195S), HaeIII (NEB, catalog no. R0108T), NdeI (NEB, catalog no. R0111S), SwaI (NEB, catalog no. R0604S),

(2) A Cell Cycle-Dependent Ferroptosis Sensitivity Switch Governed by EMP2bioRxiv : the preprint server for biologyJuly 19, 2023Rodencal J, Kim N, Li VL, He A, Lange M, He J, Tarangelo A, Schafer ZT, Olzmann JA, Sage J, et al.gift of Jan Carette (Stanford School of Medicine). pSD292 was linearized by digestion with EcoRV (Cat# R3195S, New England Biolabs, Ipswich, MA) as per manufacturer protocol. The MBOAT1 insert was cloned into pSD292

(3) Long-range DNA end resection supports homologous recombination by checkpoint activation rather than extensive homology generationeLifeJune 30, 2023Michael T Kimble, Matthew J Johnson, Mattie R Nester, Lorraine S Symingtonenzyme other,BglII,New England BioLabs,Cat# R0144S,Restriction enzyme other,EcoRV-HF,New England BioLabs,Cat# R3195S,Restriction enzyme other,rCutSmart buffer,New England BioLabs,Cat# B6004S,Restriction enzyme buffer other,NEBuffer

(4) The conserved histone chaperone Spt6 is strongly required for DNA replication and genome stabilityCell ReportsMarch 28, 2023Catherine L.W. Miller, Fred Winstonverticillus,Sigma,P9564 3-Indoleacetic acid (IAA),Sigma,12886 RnaseH,NEB,M0297L AccI,NEB,R0161S EcoRV,NEB,R3195S NcoI,NEB,R3193S HaeIII,NEB,R0108S BsrG1,NEB,R3575S SigmaFAST protease inhibitor cocktail tablets,Sigma,S8820-20TAB RNaseA/T1

(5) Retrospective identification of intrinsic factors that mark pluripotency potential in rare somatic cellsbioRxiv : the preprint server for biologyFebruary 10, 2023Jain N, Goyal Y, Dunagin MC, Cote CJ, Mellis IA, Emert B, Jiang CL, Dardani IP, Reffsin S, Raj A.restriction sites just after the stop codon of GFP. We digested this vector backbone with EcoRV (NEB #R3195S) and gel purified the resulting linearized vector. We ordered PAGE-purified ultramer oligonucleotides

(6) T-REX17 is a transiently expressed non-coding RNA essential for human endoderm formationeLifeJanuary 31, 2023Landshammer A, Bolondi A, Kretzmer H, Much C, Buschow R, Rose A, Wu HJ, Mackowiak SD, Braendl B, Giesselmann P, et al.(Promega, E6701) w/o any promoter for promoter-assays were first digested using EcoRV (New England Biolabs, R3195S). Next, full eSOX17 (Addgene plasmid # 195498), eSOX17.1 or eSOX17.2 (Addgene plasmid # 195499) for

(7) Eisosome disruption by noncoding RNA deletion increases protein secretion in yeastPNAS NexusNovember 1, 2022Matthew Wenjie Feng, Daniela Delneri, Catherine B Millar, Raymond T O'Keefevector pRS403 were digested with XhoI (NEB, catalogue number R0146S) and EcoRV (NEB, catalogue number R3195S) restriction enzymes at 37°C for 1 h. The digested plasmid was treated with 1 μL alkaline phosphatase,

(8) The SMC-family Wadjet complex protects bacteria from plasmid transformation by recognition and cleavage of closed-circular DNAMolecular CellNovember 1, 2022Amar Deep, Yajie Gu, Yong-Qi Gao, Kaori M. Ego, Mark A. Herzik, Huilin Zhou, Kevin D. Corbett,New England Biolabs,M0203S T4 Polynucleotide kinase,New England Biolabs,M0201S EcoRV-HF,New England Biolabs,R3195S Calf intestinal phosphatase,New England Biolabs,M0525S Critical commercial assays,Critical commercial

(9) Protocol for constructing large size human antibody heavy chain variable domain (VH) library and selection of SARS-CoV-2 neutralizing antibody domainsSTAR ProtocolsSeptember 17, 2021Chuan Chen, Zehua Sun, Xianglei Liu, Wei Li, Dimiter S. DimitrovScientific,BP906-5 "Dextrose (D-Glucose), Anhydrous",Fisher Scientific,D16-1 SfiI,NEB,R0123L EcoRV-HF,NEB,R3195S EcoRI-HF,NEB,R3101S Polyethylene Glycol 8000,Fisher Scientific,BP233-1 Sodium Chloride,Fisher Scientific,S271-3 PEI

(10) A new family of globally distributed lytic roseophages with unusual deoxythymidine to deoxyuridine substitutionCurrent BiologyJuly 26, 2021Branko Rihtman et al.M0280S Endonuclease VIII,NEB,Catalog # M0299S XbaI,ThermoFisher,Catalog # ER0682 EcoRV,NEB,Catalog # R3195S HindIII,ThermoFisher,Catalog # ER0501 ,, Deposited data,Deposited data,Deposited data ,, DSS3_VP1

(11) SeqStain is an efficient method for multiplexed, spatialomic profiling of human and murine tissuesCell Rep MethodsJune 21, 2021Rajagopalan A, Venkatesh I, Aslam R, Kirchenbuechler D, Khanna S, Cimbaluk D, Kordower JH, Gupta V.EcoRV de-staining buffer (deionized water containing 1X cut-smart buffer and 200 units of EcoRV (NEB, #R3195S) in 500ul), SmaI de-staining buffer (de-ionized water containing 1X cut smart buffer and 200 units of

(12) Optimizing Integration and Expression of Transgenic Bruton's Tyrosine Kinase for CRISPR-Cas9-Mediated Gene Editing of X-Linked AgammaglobulinemiaThe CRISPR journalApril 1, 2021Gray DH, Villegas I, Long J, Santos J, Keir A, Abele A, Kuo CY, Kohn DB.delivered to recognize the UC462 region of the X chromosome. One microliter of EcoRV-HF endonuclease (Cat: R3195S; New England Biolabs) was added to the reaction mixture (an enzyme that does not disrupt the experimental

(13) Stepwise accumulation of mutations in CesA3 in Phytophthora sojae results in increasing resistance to CAA fungicidesEvolutionary ApplicationsApril 1, 2021Meng Cai, Can Zhang, Weizhen Wang, Qin Peng, Xi Song, Brett M. Tyler, Xili Liua 1305‐bp fragment of the CesA3 gene from the wild‐type P. sojae isolate P6497 into the EcoRV (NEB, R3195S) site of the pBluescript II KS + vector, using the In‐Fusion® HD Cloning Kit (Clontech). The primers

(14) Engineering essential genes with a ""jump board"" strategy using CRISPR/Cas9MicroPubl BiolOctober 8, 2020Ye Duan, Sungwook Choi, Charles Nelson, Victor Ambrosafter egg laying, and the PCR products were analyzed by restriction digestion using EcoRV (NEB, Cat:R3195S) at 1 unit/µl PCR product for 30 min. In the first step (Fig. 1A), samples from the broods with jump

(15) A versatile reporter system for multiplexed screening of effective epigenome editorsNature ProtocolsOctober 1, 2020Maria Silvia Roman Azcona, Yongxing Fang, Antonio Carusillo, Toni Cathomen, Claudio MussolinoPlus DNA ladder kit (NEB, cat. no. N3200L) ● Orange G (Roth, cat. no. 0318) ● EcoRV-HF (NEB, cat. no. R3195S) ● PspOMI (NEB, cat. no. R0653S) ● CutSmart buffer (NEB, cat. no. B7204S) NATURE PROTOCOLS | VOL 15

(16) Rapid and Efficient Synthetic Assembly of Multiplex Luciferase Reporter Plasmids for the Simultaneous Monitoring of Up to Six Cellular Signaling PathwaysCurrent Protocols in Molecular BiologyJune 26, 2020Alejandro Sarrion‐Perdigones, Yezabel Gonzalez, Koen J.T. Venkenno. R0580S), EcoRI-HF (New England Biolabs, cat. no. R3101S), EcoRV-HF (New England Biolabs, cat. no. R3195S), KpnI-HF (New England Biolabs, cat. no. R3142S), SphI-HF (New England Biolabs, cat. no. R3182S), XhoI

(17) AMOTL1 enhances YAP1 stability and promotes YAP1-driven gastric oncogenesisOncogeneMay 28, 2020Yuhang Zhou et al.ATC TAG CTT GG-3′. According to homologous recombination, between site SalI (R3138S, NEB) and EcorV (R3195S, NEB), the fragments were subsequently inserted into pENTR1A, from which the DEST vectors were created

(18) Determination of Chemical Inhibitor Efficiency against Intracellular Toxoplasma Gondii Growth Using a Luciferase-Based Growth AssayJournal of Visualized ExperimentsMay 19, 2020Melanie Key, Amy Bergmann, Chiara Micchelli, L. Brock Thornton, Sophie Millard, Zhicheng DouHiFi PCR premix,Takara Bio,639298, Coelenterazine h,Prolume,301-10 hCTZ, EcoRV,New England BioLabs,R3195S, Phire Tissue Direct PCR Master Mix,Thermo Scientific,F170L, Plasmid miniprep kit,Zymo Research,D4054, Q5 Site-Directed Mutagenesis kit

(19) Dual Fluorescence Cytometry Assay to Assess Cellular Protein LevelsBIO-PROTOCOLApril 20, 2020Ashley M Ngo, Ruofan Wang, Andreas S PuschnikKaufman; Addgene plasmid, catalog number: 17452) 16. EcoRV-HF (New England Biolabs, catalog number: R3195S) 17. Quick CIP (New England Biolabs, catalog number: M0525S) 18. S.O.C. Medium (Teknova, catalog number:

(20) Integrative analysis suggests cell type-specific decoding of NF-κB dynamicsScience signalingFebruary 25, 2020Martin EW, Pacholewska A, Patel H, Dashora H, Sung MH.pSF-EF1α-Ub-Neo plasmid were digested with restriction enzymes from NEB (EcoR1-HF, R3101S; EcoRV-HF, R3195S; BsiW1, R0553S; BsrGI-HF, R3575S), where applicable. The plasmid was dephosphorylated with Antarctic Phosphatase

Write a Review

(1)

10/7/2015High Fidelity Restriction Enzyme for Cloning Prabhakaran KumarI used this EcoRV restriction enzyme along with BglII for the cloning of my gene of interest into a plasmid vector. Subsequently I used this enzyme for colony screening also. This enzyme completely ... Read Review

EcoRV-HF

EcoRV-HF from New England Biolabs

Description

Sequence

New England Biolabs

Citations (26)

Reviews

EcoRV-HF from New England Biolabs

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map