EcoRV from New England Biolabs

Product Specs
ItemEcoRV
CompanyNew England Biolabs
Price $273.00 Inquire
Catalog NumberR0195L
Concentration20-100 u/µL
Quantity20000 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

GAT/ATC

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Impact of Tau overexpression on DNA replication dynamics in centromeres of human neural progenitor cellsiScienceFebruary 20, 2026Elisa Balzano, Shyam Twayana, Settapong T. Kosiyatrakul, Glennis A. Logsdon, Bhushan L. Thakur, Evan E. Eichler, Bettina Bohl, Philipp Koch, Simone Sidoli, Anupama Kumari, et al.with modifications to detect rDNA repeats. pB plasmid described in78 was digested with EcoRV (NEB, R0195S) and labeled with digoxigenin using BioPrime DNA Labeling System (Ref# 18094-011). The biotinylated

(2) Ribosome-induced mRNA pseudoknot interactions visualized by DMS MaP-SeqbioRxivOctober 6, 2025Preston A. Kellenberger, Zhenwei Song, Xiao Heng, Peter V. Cornishand ligation independent cloning (SLIC). Purification and digestion of the plasmid with EcoRV (NEB, R0195S) produced a linear DNA fragment containing the T7 promoter and ScYLV sequence (Supporting Information).

(3) Kif11-haploinsufficient oocytes reveal spatially differential requirements for chromosome biorientationEMBO ReportsAugust 20, 2025Tappei Mishina, Aurélien Courtois, Shuhei Yoshida, Kohei Asai, Hiroshi Kiyonari, Tomoya S Kitajimakit,invitrogen,AM1344 3-isobutyl-1-methyl-xanthine,Sigma-Aldrich,15879 monastrol,Sigma-Aldrich,M815 EcoRV,New England Biolabs,R0195S SNAP-Cell 647-SiR,New England Biolabs,S9102S Hoechst 33342,Invitrogen,H3570 SMART-Seq v4 Ultra Low

(4) Quantitative essentiality in a reduced genome: a functional, regulatory and structural fitness mapMolecular Systems BiologyAugust 13, 2025Samuel Miravet-Verde, Raul Burgos, Eva Garcia-Ramallo, Marc Weber, Luis Serranoreagents" Puromycin,Gibco,A1113803 Chloramphenicol,Sigma,C0378 Tetracycline,Sigma,T7660 Gentamicin,Sigma,G1397 Anhydrotetracycline,Takara,631310 BamHI,NEB,R0136S EcoRV,NEB,R0195S Phusion High-Fidelity DNA Polymerase,Thermo Scientific,F530S HEPES,Sigma,H4034 Sucrose,Sigma,84097 Glucose,Sigma,G-8270 PPLO

(5) Skin mycobiota-mediated antagonism against Staphylococcus aureus through a modified fatty acidCurrent BiologyMay 19, 2025Caitlin H. Kowalski, Uyen Thy Nguyen, Susannah Lawhorn, T. Jarrod Smith, Rebecca M. Corrigan, Won Se Suh, Lindsay Kalan, Matthew F. Barberpolymerase,NEB,Cat#M0530S T4 Polynucleotide Kinase,NEB,Cat#M0201S SmaI,NEB,Cat#R0141S EcoRV,NEB,Cat#R0195L XbaI,NEB,Cat#R0145S Novagen Pellet Paint Co-precipitant,EMB Millipore,Cat#69049-3 Quick Ligation

(6) Dynamic sampling of a surveillance state enables DNA proofreading by Cas9Cell Chemical BiologyFebruary 20, 2025Viviane S. De Paula, Abhinav Dubey, Haribabu Arthanari, Nikolaos G. Sgourakismg of pNL003 plasmid59 was linearized by incubation at 37 °C for 2–4 h in the presence of EcoRV (NEB R0195S). Transcription reactions (30 mL) were conducted in buffer containing 50 mM Tris, pH 7.9, 25 mM MgCl2,

(7) Enhanced replication of a hepatitis A virus vaccine strain via adaptation in Vero cellsClinical and Experimental Vaccine ResearchJanuary 22, 2025Sang Hwan Seo, Jung-ah Choi, Mi Sun Kim, Eunji Yang, Sumin Choi, Dong Won Seo, Manki Songplasmid DNA was linearized using MluI (R0198S; New England Biolabs [NEB], Ipswich, MA, USA) and EcoRV (R0195S; NEB), and the linearized DNA was purified using a QIAquick PCR & Gel Cleanup Kit (#28506; Qiagen, Hilden,

(8) Training data diversity enhances the basecalling of novel RNA modification-induced nanopore sequencing readoutsNat CommunJanuary 15, 2025Ziyuan Wang, Ziyang Liu, Yinshan Fang, Hao Helen Zhang, Xiaoxiao Sun, Ning Hao, Jianwen Que, Hongxu Dingcollection. Specifically, curlcake-containing pUC57 plasmids were digested using EcoRV (New England Biolabs, R0195L) and BamHI (New England Biolabs, R0136L) restriction enzymes for at least two hours at 37 °C, and further

(9) Novel role of zinc-finger protein 518 in heterochromatin formation on α-satellite DNANucleic Acids ResearchJanuary 11, 2025Shinya Ohta, Jun-Ichirou Ohzeki, Nobuko Sato, Hideki Tanizawa, Claire Yik-Lok Chung, Ken-Ichi Noma, Hiroshi Masumoto#A10465, Thermo Fisher Scientific) between the XmnI (#R0194S, New England BioLabs [NEB]) and EcoRV (#R0195S, NEB) sites using the In-Fusion HD Cloning Kit (#Z9648N, Takara Bio), yielding entry plasmids pENTR4XE153,

(10) Efficient sex hormone biosensors in Saccharomyces cerevisiae cells to evaluate human aromatase activity and inhibitionSci RepJanuary 3, 2025Jie Wu, Matthias Bureik, Mario Andrea Marchisionucleotide sequences were synthesized by GENEWIZ (Soochow, China). Acc651 (R0599S), BstXI (R0113S), EcoRV (R0195S), Sacl (R0156S), Stul (R0187S) restriction enzymes, O5 Hot-start polymerase (M0491S), T4 DNA ligase

(11) Allele-specific DNA demethylation editing leads to stable upregulation of allele-specific gene expressioniScienceOctober 18, 2024Nivethika Rajaram, Katharina Benzler, Pavel Bashtrykov, Albert Jeltschng of the genomic DNA was subjected to an overnight enzymatic fragmentation by EcoRV (NEB Catalog #R0195L) which does not cleave within any of the genomic regions of interest. The digested genomic DNA was bisulfite

(12) Rli51 Attenuates Transcription of the Listeria Pathogenicity Island 1 Gene mpl and Functions as a Trans-Acting sRNA in Intracellular BacteriaInternational Journal of Molecular SciencesAugust 29, 2024Álvaro Morón, Laura Ortiz-Miravalles, Marcos Peñalver, Francisco García-del Portillo, M. Graciela Pucciarelli, Alvaro Darío Ortega(M0202S), and restriction enzymes (KpnI: R3142S, SalI: R3138S, EcoRI: R3101S, BglII: R0144S, EcoRV: R0195S, DpnI: R0176S) were supplied by New England Biolabs. Kits for extracting plasmidic DNA (NucleoSpin Plasmid:

(13) Jorvik: A membrane-containing phage that will likely found a new family within VinaviralesiScienceNovember 17, 2023Pavol Bárdy, Conor I.W. MacDonald, Roman Pantůček, Alfred A. Antson, Paul C.M. Foggstain,NBS Biologicals,Cat#NBS-SB1L Dnase I,, PEG8000,Promega,Cat#V3011 EcoRV,New England Biolabs,Cat#R0195S ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, NEBuilder

(14) Visualization of Actin Cytoskeleton in Cellular Protrusions in Medaka EmbryosBIO-PROTOCOLJuly 5, 2023Toru Kawanishi, Ann Kathrin Heilig, Atsuko Shimada, Hiroyuki Takedanumber: R0152S). 10× CutSmart buffer is included in the productEcoRV (New England Biolab, catalog number: R0195S). 10× CutSmart buffer is included in the product0.5% phenol red in DPBS (Sigma-Aldrich, catalog number:

(15) Sparse and stereotyped encoding implicates a core glomerulus for ant alarm behaviorCellJuly 1, 2023Taylor Hart, Dominic D. Frank, Lindsey E. Lopes, Leonora Olivos-Cisneros, Kip D. Lacy, Waring Trible, Amelia Ritger, Stephany Valdés-Rodríguez, Daniel J. C. Kronauer#104875),122 from double restriction digest with SpeI (New England Biolabs [NEB] #R3133S) and EcoRV (NEB #R0195S). ie1-A: An enhancer/promoter from pGL3-IE1 (Addgene ID #52894)62 (primers: forward 5’- ttatcgaattcctgcagcccgggggatccaACTAGTTGTTCGCCGAGCTCTTACGCGC

(16) A transgenic mouse line for assaying tissue-specific changes in endoplasmic reticulum proteostasisTransgenic researchJune 1, 2023Svarcbahs R, Blossom SM, Baffoe-Bonnie HS, Trychta KA, Greer LK, Pickel J, Henderson MJ, Harvey BK.E. Bergles (Agarwal et al. 2017). Briefly, backbone was digested with MluI (#R0198S; NEB) and EcoRV (R0195S; NEB), gel purified and recombined with inserts using an In-Fusion HD cloning kit (639,645, Clontech).

(17) Insights into the secondary and tertiary structure of the Bovine Viral Diarrhea Virus Internal Ribosome Entry SiteRNA BiologyDecember 31, 2022Devadatta Gosavi, Iwona Wower, Irene K. Beckmann, Ivo L. Hofacker, Jacek Wower, Michael T. Wolfinger, Joanna Sztuba-Solinskawhile the 3′ end pair added an EcoRV site. Double digestion with EcoRI (NEB, R0101S) and EcoRV (NEB, R0195S) was used for the initial screening of clones. All putative correct clones were verified by sequencing.

(18) DNA methyltransferases are complementary in maintaining DNA methylation in embryonic stem cellsiScienceSeptember 16, 2022Liu Y, Xu Z, Shi J, Zhang Y, Yang S, Chen Q, Song C, Geng S, Li Q, Li J, et al.dye,"Tiangen, China",Cat. # RT210 EDTA,"Amethyst,China",Cat. # 976151-500G EcoRV,"NEB, USA",Cat. #R0195S HindIII,"NEB, USA",Cat. #R0104S TaqI,"Monad, China",Cat. # MF02801S ClaI,"Monad, China",Cat. # MF00501M HhaI,"NEB,

(19) Interspecies effectors of a transgenerational memory of bacterial infection in Caenorhabditis elegansiScienceJuly 15, 2022Marcela LegÃ¼e, Mauricio Caneo, Blanca Aguila, Bernardo Pollak, Andrea CalixtoL4440-rsmZ A 500 ng mass of L4440 vector (Fire et al., 1998) was digested using EcoRV (NEB Cat. No. R0195S) and dephosphorylated using Antarctic Phosphatase (NEB Cat. No. M0289S) according to the manufacturer’s

(20) Efficient isolation of mouse deletion mutant embryonic stem cells by CRISPRSTAR ProtocolsJune 17, 2022Yuhan Liu, Qian Chen, Chenglin Song, Zhen Xu, Shuting Yang, Xiajun LiChina",A88490 GeneGreen nucleic acid dye,"TIANGEN, China",RT210 EDTA,"Amethyst, China",976151-500G EcoRV,"NEB, USA",R0195S HindIII,"NEB, USA",R0104S ,, Critical commercial assays,Critical commercial assays,Critical commercial

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EcoRV

EcoRV from New England Biolabs

Sequence

New England Biolabs

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EcoRV from New England Biolabs

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