EcoRI-HF from New England Biolabs

Product Specs
ItemEcoRI-HF
CompanyNew England Biolabs
Price $64.00 Inquire
Catalog NumberR3101S
Quantity10000 units
Concentration20,000 units/ml
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

G⇓AATTC
C⇑CATGG

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Presentation of identical tumor antigen peptides by canine and human MHC class IiScienceJune 19, 2026Saghar Kaabinejadian, Hooman Yari, Steven Cate, William Berry, Mengyuan Zhang, Hannah Lorelle Cavanagh, Shaying Zhao, William H. Hildebrand5,Polysciences,Cat# 26008 Xho1,NEB,Cat# R0146S Salt-T4 DNA Ligase,NEB,Cat# M0467S EcoR1,NEB,Cat# R3101S LR Clonase II,ThermoFisher,Cat# 11791020 Polybrene,Sigma-Aldrich,Cat# TR-1003-50UL Blasticidin,ThermoFisher,Cat#

(2) Mutual potentiation of IPA1 and OsNPR1 enhances rice immunityPlant CellMay 20, 2026Zhen Wang, Mingming Liu, Rujuan Yan, Xiaohan Zhang, Xiaoguang Song, Guosheng Xiong, Jiayang Li, Zuhua He, Dong-Lei Yangfragment of OsNPR1 was cloned into the pCAMBIA1305-3xFLAG vector, which had been digested with EcoRI (R3101S, NEB) and KpnI (R3142S, NEB) enzymes, using T4 DNA ligase (M0202V, NEB). For OsNPR1-GFP, the coding

(3) A Wnt-induced conformational phospho-switch in DVL3 controls association with Frizzled receptors and Wnt/β-catenin signalingSci AdvMay 15, 2026Miroslav Micka, Jitender Kumar, Petra Paclíková, Zuzana Hayek, Kateřina Hanáková, Cherine Bechara, Hana Plešingerová, Ondrej Šedo, Sara Bologna, Elise Del Nero, et al.via restriction digestion between the 5′-NcoI and 3′-EcoRI sites, using NcoI (R0193L, NEB) and EcoRI (R3101S, NEB) restriction enzymes. The DNA segment encoding CK1ε core was excised from our in-house pETM11-CK1ε

(4) ThermiQuant™ AquaStream: A portable instrument for quantitative colorimetric isothermal nucleic acid amplification reactions in paper and tube formatsPLoS OneMay 15, 2026Bibek Raut, Gopal Palla, Jiangshan Wang, Skyler Campbell, Virendra Kumar, Kyungyeon Ra, Mohit S. VermaFAM-labeled probe; see S3 Table in S1 File), 0.5 μL of EcoRI-HF restriction enzyme (New England Biolabs, R3101S), 20.5 μL of nuclease-free water, and 5 μL of DNA template. Reaction mixtures were loaded into a 26K

(5) Rhythmic protein condensation in the circadian regulation of glycogen metabolismiScienceMay 15, 2026Lily A. Burton, Gopal K. Pattanayak, Michael J. Rustproteins" ,, Q5 High-Fidelity 2X Master Mix,New England Biolabs,Cat# M0492S EcoRI-HF,New England Biolabs,Cat# R3101S Gibson Assembly Master Mix,New England Biolabs,Cat# E2611S "cOmplete, EDTA free cocktail tablets",Sigma-Aldrich,Cat#

(6) A proximity-labeling map of PI5P4K phosphoinositide kinases interaction networksJournal of Biological ChemistryApril 25, 2026Alicia Llorente, Gurpreet K. Arora, Ryan M. Loughran, Rabi Murad, Svetlana Maurya, Sophia Crabtree, Benji Portillo, Pratima Chapagain, Hundaol Huluka, Scott Eron, et al.plasmid #91980, https://www.addgene.org/91980/) using NheI and EcoRI restriction enzymes (R3131S and R3101S, New England Biolabs), Phusion Hot Start II DNA Polymerase (F549S, Thermo Fisher Scientific) and In-Fusion

(7) Evaluating splicing factor and kinase network crosstalk through global phosphoproteomicsbioRxivApril 24, 2026Sam Crowl, Sameek Singh, Tian Zhang, Kristen M. Naegleremoved from the pLKO backbone by cutting with EcoR1 and Kpn1 restriction enzymes (New England Biolabs #R3101S, #R0142S). The pLKO backbone was isolated via gel electrophoresis and extracted with the Qiagen Gel

(8) T-cell protrusions enable fast, localised initiation of chimeric antigen receptor signallingThe EMBO JournalApril 21, 2026Carmen Rodilla-Ramirez, Giorgia Carai, Eleanor Fox, Amin Zehtabian, Helen Adam, Katja Dallio, Pia Lazki-Hagenbach, Helge Ewers, Xiaolei Su, Francesca Bottanellilab Enzymes,Enzymes,Enzymes BamHI-HF,New England Biolabs (NEB),R3131S BpiI,Thermo Fisher,FD1014 EcoRI-HF,NEB,R3101S HindIII-HF,NEB,R3104S Nhe-HF,NEB,R3131S NotI,NEB,R3189S Pfu polymerase,Roboklon,E1114 Quick CIP

(9) Single-cell Analysis Reveals Essential lncRNAs Regulating Human Trophoblast Lineage DifferentiationLife MedicineApril 17, 2026Weiyi Zhang, Chunyan Le, Shanru Yi, Wenqiang Liu, Xiaoyu Liu, Zhen Yin, Hong Wang, Rui Gao, Shaorong Gao, Jiayu Chenlinearized by double digestion with AgeI (New England Biolabs, R3552L)/EcoRI (New England Biolabs, R3101S) restriction endonuclease and purified for subsequent cloning. Using an online website DSIR (biodev.extra.cea.fr/DSIR/DSIR.html)

(10) Unique DNA impacts on transient Expi293TMPRO expression for enhancing biotherapeutic protein productionFront Bioeng BiotechnolApril 14, 2026Bo Hee Shin, Jing Zhou, Audrey M. Vogt, Lauren Gebhardt, Molica Abel, Hamza Sahil, Fabrice Lamour, Anna Kimeu, Ian R. Wallace-Moyer, Sean Lim, et al.with restriction enzyme cutting with EcoRI-HF (New England BioLabs, Ipswich, MA, United States, Cat. #R3101S) and BamHI-HF (New England BioLabs, Cat. #R3136L). The final constructs were verified by Nanopore sequencing

(11) Synthetic circRNAs employ IRES activity for translation in cells and in cell-free translation systemsbioRxivMarch 30, 2026Philipp Koch, Frederic S. W. Arendrup, Chaehee Lim, Samyukta Narayanan, Amina Adam, Massimiliano Clamer, Anders H. Lund, Chun-Kan Chen, Kathrin Leppekrecommendations (NEB Q5 Polymerase, M0493S). The plasmid backbone was digested using EcoRI-HF (NEB, R3101S) and BsiWI-HF (NEB, R3553S) restriction enzymes according to manufacturer’s instructions. The Gibson

(12) GTPase Rab11b and effector Rab11-FIP2 promote NLRP3 stability during inflammasome primingThe EMBO JournalMarch 25, 2026Caroline S Gravastrand, Maria Yurchenko, Stine Kristensen, Astrid Skjesol, Carmen Chen, Sindre Ullmann, Zunaira Iqbal, Karoline Ruud Dahlen, Kashif Rasheed, Unni Nonstad, et al.recommendations for three DNA fragments. The kit was used to combine the EcoRI-HF (New England Biolabs, R3101S) cleaved empty pCMV-DYKDDDDK-N vector (Takara Bio, 635688) with 2 PCR-products to change the NLRP3 KMKK

(13) Hemocyte-secreted Papilin bearing mucin-type O-glycans regulates peripodial stalk formation via epidermal JAK/STAT signaling in DrosophilaiScienceMarch 20, 2026Takashi J. Fuwa, Kazuyoshi Itoh, Tomomi Ichimiya, Yoshihiro Akimoto, Shoko NishiharaFluor-647-conjugated streptavidin,Thermo Fisher Scientific,Cat# S21374 EcoRI-HF,New England Biolabs,Cat# R3101S KpnI-HF,New England Biolabs,Cat# R3142S XhoI,New England Biolabs,Cat# R0146S Cellfectin™ II Reagent,Thermo Fisher Scientific

(14) Disease-causing mutations in Synaptotagmin can act via dominant-negative, gain-of-function or haploinsufficient mechanismsbioRxivMarch 19, 2026Zhuo Guan, Maria Bykhovskaia, J. Troy Littletonassays QuikChange Lightning Site-Directed Mutagenesis Kit,Agilent,Cat# 210518 EcoRI-HF,New England BioLabs,Cat# R3101S XbaI,New England BioLabs,Cat# R0145S Experimental models: Organisms/strains,Experimental models: Organisms/strains,Experimental models

(15) Interdependent RNA structural motifs at the 3ʹ-terminus of the West Nile virus genome regulate viral growthbioRxivMarch 14, 2026Lucille H. Tsao, Doug E. Brackney, Anna Marie PyleSLII and DBI mutants, 3’-Terminus) (NEB, cat #R0145S) and EcoRI (SLIV and DBII mutants) (NEB, cat #R3101S). The linearized plasmid served as a template for in vitro transcription in conditions previously described

(16) Armored macrophage-targeted CAR-T cells reset and reprogram the tumor microenvironment and control metastatic cancer growthCancer CellMarch 9, 2026Jaime Mateus-Tique, Ashwitha Lakshmi, Bhavya Singh, Rhea Iyer, Alfonso R. Sánchez-Paulete, Chiara Falcomatà, Matthew Lin, Gvantsa Pantsulaia, Alexander Tepper, Trung Nguyen, et al.Bioscience (CA, USA) and cloned between the CD3z domain and the T2A-GFP sequence using using EcorI-HF (NEB # R3101S) and HindIII-HF (NEB R3104S) for their respective restriction sites. For the CMV driven IL-12 vector,

(17) Cytosine base editing of LPA in transgenic mice averts large deletionsMol TherMarch 3, 2026Marcel A. Chuecos, So Hyun Park, Madhvi M. Bhakta, Usosa Too-Chiobi, Daniel Betancourth, Mingming Cao, Marco De Giorgi, Christopher J. Walkey, Anjana Tiwari, Biana Godin, et al.total of 400 ng of HEK293-Apo(a) genomic DNA was digested with EcoRI-HF (New England Biolabs, cat. #R3101S) prior to PCR. EvaGreen droplet digital PCR reactions were performed to quantify the copy number of

(18) Hypofucosylation promotes pertussis toxin binding to cell surface glycococonjugates and pertussis toxin-induced intracellular ERK signalingGlycobiologyMarch 3, 2026Rohit Sai Reddy Konada, Nicole Nischan, Aurora Silva, Jennifer J KohlerpLVX-IRES-puromycin vector (Takara Bio, cat # 632183) linearized by EcoRI-HF and BamHI-HF (NEB, cat # R3101S and R3136S) using HiFi DNA assembly master mix (NEB cat # E5520S). The FUT3 expression plasmid also

(19) Viral and Host Factors Involved in Host Gain and Host Loss by Tomato Leaf Curl Begomoviruses in Tomato and CucumbersMol Plant PatholFebruary 27, 2026Svenning Rune Möller, M. N. Maruthiwith primer p330 and p378. pGBKT7‐BD was linearized with EcoRI‐HF and BamHI‐HF (New England Biolabs: R3101S and R3136S). Full length cDNA fragments of plant genes were obtained using CloneAmp HiFi PCR Premix

(20) Fn14 is an activity-dependent, Bmal1-regulated cytokine receptor that induces rod-like microglia and restricts neuronal activity in vivoCell ReportsFebruary 24, 2026Austin Ferro, Dominic J. Vita, Trevor Fallon, Anosha Arshad, Leah Boyd, Tess Stanley, Qianyu Lin, Adrian Berisha, Uma Vrudhula, Adrian M. Gomez, et al.elongation and amplification, the eBFP2 insert was assembled into Addgene plasmid 50465 after EcoRI-HF (NEB:R3101s) and BamHI-HF (NEB:R3136s) restriction digest using a Gibson reaction (NEB: E2621S). The subsequent

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(1)

10/1/2015High Fidelity Restriction Enzyme for Cloning Prabhakaran KumarI used this EcoRI-HF restriction enzyme for one way (Single enzyme digestion) cloning of my gene of interest in to plasmid vector. Subsequently I used this enzyme for colony screening also. This ... Read Review

EcoRI-HF

EcoRI-HF from New England Biolabs

Description

Sequence

New England Biolabs

Citations (309)

Reviews

EcoRI-HF from New England Biolabs

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