EcoRI-HF from New England Biolabs

Product Specs
ItemEcoRI-HF
CompanyNew England Biolabs
Price $64.00 Inquire
Catalog NumberR3101S
Quantity10000 units
Concentration20,000 units/ml
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

G⇓AATTC
C⇑CATGG

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Rhythmic protein condensation in the circadian regulation of glycogen metabolismiScienceMay 15, 2026Lily A. Burton, Gopal K. Pattanayak, Michael J. Rustproteins" ,, Q5 High-Fidelity 2X Master Mix,New England Biolabs,Cat# M0492S EcoRI-HF,New England Biolabs,Cat# R3101S Gibson Assembly Master Mix,New England Biolabs,Cat# E2611S "cOmplete, EDTA free cocktail tablets",Sigma-Aldrich,Cat#

(2) Single-cell Analysis Reveals Essential lncRNAs Regulating Human Trophoblast Lineage DifferentiationLife MedicineApril 17, 2026Weiyi Zhang, Chunyan Le, Shanru Yi, Wenqiang Liu, Xiaoyu Liu, Zhen Yin, Hong Wang, Rui Gao, Shaorong Gao, Jiayu Chenlinearized by double digestion with AgeI (New England Biolabs, R3552L)/EcoRI (New England Biolabs, R3101S) restriction endonuclease and purified for subsequent cloning. Using an online website DSIR (biodev.extra.cea.fr/DSIR/DSIR.html)

(3) Unique DNA impacts on transient Expi293TMPRO expression for enhancing biotherapeutic protein productionFront Bioeng BiotechnolApril 14, 2026Bo Hee Shin, Jing Zhou, Audrey M. Vogt, Lauren Gebhardt, Molica Abel, Hamza Sahil, Fabrice Lamour, Anna Kimeu, Ian R. Wallace-Moyer, Sean Lim, et al.with restriction enzyme cutting with EcoRI-HF (New England BioLabs, Ipswich, MA, United States, Cat. #R3101S) and BamHI-HF (New England BioLabs, Cat. #R3136L). The final constructs were verified by Nanopore sequencing

(4) Synthetic circRNAs employ IRES activity for translation in cells and in cell-free translation systemsbioRxivMarch 30, 2026Philipp Koch, Frederic S. W. Arendrup, Chaehee Lim, Samyukta Narayanan, Amina Adam, Massimiliano Clamer, Anders H. Lund, Chun-Kan Chen, Kathrin Leppekrecommendations (NEB Q5 Polymerase, M0493S). The plasmid backbone was digested using EcoRI-HF (NEB, R3101S) and BsiWI-HF (NEB, R3553S) restriction enzymes according to manufacturer’s instructions. The Gibson

(5) Hemocyte-secreted Papilin bearing mucin-type O-glycans regulates peripodial stalk formation via epidermal JAK/STAT signaling in DrosophilaiScienceMarch 20, 2026Takashi J. Fuwa, Kazuyoshi Itoh, Tomomi Ichimiya, Yoshihiro Akimoto, Shoko NishiharaFluor-647-conjugated streptavidin,Thermo Fisher Scientific,Cat# S21374 EcoRI-HF,New England Biolabs,Cat# R3101S KpnI-HF,New England Biolabs,Cat# R3142S XhoI,New England Biolabs,Cat# R0146S Cellfectin™ II Reagent,Thermo Fisher Scientific

(6) Interdependent RNA structural motifs at the 3ʹ-terminus of the West Nile virus genome regulate viral growthbioRxivMarch 14, 2026Lucille H. Tsao, Doug E. Brackney, Anna Marie PyleSLII and DBI mutants, 3’-Terminus) (NEB, cat #R0145S) and EcoRI (SLIV and DBII mutants) (NEB, cat #R3101S). The linearized plasmid served as a template for in vitro transcription in conditions previously described

(7) Armored macrophage-targeted CAR-T cells reset and reprogram the tumor microenvironment and control metastatic cancer growthCancer CellMarch 9, 2026Jaime Mateus-Tique, Ashwitha Lakshmi, Bhavya Singh, Rhea Iyer, Alfonso R. Sánchez-Paulete, Chiara Falcomatà, Matthew Lin, Gvantsa Pantsulaia, Alexander Tepper, Trung Nguyen, et al.Bioscience (CA, USA) and cloned between the CD3z domain and the T2A-GFP sequence using using EcorI-HF (NEB # R3101S) and HindIII-HF (NEB R3104S) for their respective restriction sites. For the CMV driven IL-12 vector,

(8) Hypofucosylation promotes pertussis toxin binding to cell surface glycococonjugates and pertussis toxin-induced intracellular ERK signalingGlycobiologyMarch 3, 2026Rohit Sai Reddy Konada, Nicole Nischan, Aurora Silva, Jennifer J KohlerpLVX-IRES-puromycin vector (Takara Bio, cat # 632183) linearized by EcoRI-HF and BamHI-HF (NEB, cat # R3101S and R3136S) using HiFi DNA assembly master mix (NEB cat # E5520S). The FUT3 expression plasmid also

(9) Cytosine base editing of LPA in transgenic mice averts large deletionsMol TherMarch 3, 2026Marcel A. Chuecos, So Hyun Park, Madhvi M. Bhakta, Usosa Too-Chiobi, Daniel Betancourth, Mingming Cao, Marco De Giorgi, Christopher J. Walkey, Anjana Tiwari, Biana Godin, et al.total of 400 ng of HEK293-Apo(a) genomic DNA was digested with EcoRI-HF (New England Biolabs, cat. #R3101S) prior to PCR. EvaGreen droplet digital PCR reactions were performed to quantify the copy number of

(10) Viral and Host Factors Involved in Host Gain and Host Loss by Tomato Leaf Curl Begomoviruses in Tomato and CucumbersMol Plant PatholFebruary 27, 2026Svenning Rune Möller, M. N. Maruthiwith primer p330 and p378. pGBKT7‐BD was linearized with EcoRI‐HF and BamHI‐HF (New England Biolabs: R3101S and R3136S). Full length cDNA fragments of plant genes were obtained using CloneAmp HiFi PCR Premix

(11) Fn14 is an activity-dependent, Bmal1-regulated cytokine receptor that induces rod-like microglia and restricts neuronal activity in vivoCell ReportsFebruary 24, 2026Austin Ferro, Dominic J. Vita, Trevor Fallon, Anosha Arshad, Leah Boyd, Tess Stanley, Qianyu Lin, Adrian Berisha, Uma Vrudhula, Adrian M. Gomez, et al.elongation and amplification, the eBFP2 insert was assembled into Addgene plasmid 50465 after EcoRI-HF (NEB:R3101s) and BamHI-HF (NEB:R3136s) restriction digest using a Gibson reaction (NEB: E2621S). The subsequent

(12) Enhanced editing of Bifidobacterium lactis using the endogenous Type I-G CRISPR-Cas systemApplied and Environmental MicrobiologyFebruary 18, 2026Ourania Raftopoulou, Kendall Malmstrom, Meichen Pan, Rodolphe Barrangouwas digested with BamHI (NEB, Ipswich, MA, USA; Cat# R3136S) and EcoRI (NEB, Ipswich, MA, USA; Cat# R3101S) to replace the spectinomycin resistance gene with a chloramphenicol resistance cassette (promoter-cat-terminator),

(13) Systematic annotation of orphan RNAs reveals blood-accessible molecular barcodes of cancer identity and cancer-emergent oncogenic driversCell Reports MedicineFebruary 17, 2026Jeffrey Wang, Jung Min Suh, Brian J. Woo, Albertas Navickas, Kristle Garcia, Keyi Yin, Lisa Fish, Taylor Cavazos, Benjamin Hänisch, Daniel Markett, et al.for 90 min using AgeI-HF (New England Biolabs Cat. #R3552S) and EcoRI-HF (New England Biolabs Cat. #R3101S). Digested inserts were then run on a 8% TBE gel, extracted, and then ethanol precipitated overnight

(14) USP30-mediated Deubiquitination of Hexokinase 2 controls the metabolic fate of glucose and tumor progressionCell Death & DiseaseFebruary 14, 2026Zhang Haowei, Xiaolin Li, Weijie Liao, Xuan Qin, Yapei Jiang, Haitao Yang, Hongli Zeng, Yuetong Li, Weidong Xie, Yaou Zhang, et al.cloned into the pLKO.1-TRC vector (Addgene, #10878) digested with AgeI (NEB, R3552S) and EcoRI (NEB, R3101S). The resulting plasmids were verified by restriction enzyme digestion and DNA sequencing (BGI). Lentivirus

(15) Evolution of Translation Initiation Factor 2 Extensions Links Initiation to Bacterial Stress ResponsebioRxivFebruary 11, 2026Evrim Fer, Rajdeep Banerjee, Katsumi Hagino, Kaitlyn M. McGrath, Betül Kaçargene and ΔN IF2 (Δ1–294) genes were cloned into pET21a(+) using HindIII-HF (NEB-R3104S) and EcoRI (NEB-R3101S) restriction enzymes. For in vivo experiments, pTrcHisB were used as backbone. E. coli infB gene, ΔN

(16) Regulatory grammar in human promoters uncovered by MPRA-based deep learningNatureFebruary 4, 2026Lucía Barbadilla-Martínez, Noud Klaassen, Vinícius H. Franceschini-Santos, Jérémie Breda, Hatice Yücel, Miguel Hernández-Quiles, Tijs van Lieshout, Carlos G. Urzua Traslaviña, Minh Chau Luong Boi, Maryam Akbarzadeh, et al.DNA End-Repair kit (Lucigen, ER81050), subsequently bead-purified and digested with EcoRI-HF (NEB, R3101S) and NheI-HF (NEB, R3131S) for 1 h at 37 °C. Fragments were ligated in NheI-HF/EcoRI-HF double-digested

(17) Protein kinase a regulates cyclooxygenase-2 expression through the RNA-binding proteins HuR and TTPJournal of Biological ChemistryFebruary 1, 2026Sendi Rafael Adame-Garcia, Thomas S. Hoang, Pham Thuy Thien Vo, Valeria Burghi, Rodolfo Daniel Cervantes-Villagrana, Lennis Beatriz Orduña-Castillo, Dana J. Ramms, JoAnn Trejo, J. Silvio GutkindpHTN-HaloTag-CMV-neo-Vector (Promega, G7721) using Gibson Assembly. The vector was previously digested with EcoRI-HF (NEB, R3101S), dephosphorylated, and purified. A complete list of primers used for cloning and mutagenesis is provided

(18) Cancer cachexia in STK11/LKB1-mutated non-small cell lung cancer is dependent on tumor-secreted GDF15Nature CommunicationsJanuary 30, 2026Jinhai Yu, Tong Guo, Arun Gupta, Ernesto M. Llano, Thomas Salisbury, Naureen Wajahat, Dianne Zhao, Sean Slater, Qing Deng, Esra A. Akbay, et al.into the pLVX-EF1α-IRES-Puro vector (Clontech, #631988) by first treating with the EcoR1-HF (NEB, #R3101S) and BamH1-HF (NEB, #R3136S) restriction enzymes followed by In-Fusion Snap Assembly (Takara, #638947)

(19) Integrating traditional omics and AI-driven approaches for discovery and validation of novel MicroRNA biomarkers and therapeutic targets in thyroid cancerFrontiers in PharmacologyJanuary 28, 2026Yi Wan, Dan Xie, Min Zhang, Shiyu Yang, Zhantian Zhang, Xiaomin Fu, Meiling Wang, Yongfu ZhaoThe vector and insert were digested using EcoRI-HF and BamHI-HF restriction enzymes (catalog numbers R3101S and R3136S, respectively, New England Biolabs, Ipswich, MA, USA) at 37 °C for 2 h. Ligation was performed

(20) A prophage-expressed type IV pilus component provides anti-phage defenseCell ReportsJanuary 27, 2026Kristina M. Sztanko, Taylor J. Ellison, Jill Greenlaw, Tatiana Lenskaia, P. Nathael Javorčík, Alexa D. Fitzpatrick, Karen L. Maxwell, Courtney K. Ellison, Alan R. Davidson,Cell Signaling Technologies,Cat# 51854S NcoI-HF,New England Biolabs,Cat# R3193S EcoRI-HF,New England Biolabs,Cat# R3101S HindIII-HF,New England Biolabs,Cat# R3104S KpnI-HF,New England Biolabs,Cat# R3142S XbaI-HF,New England Biolabs

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10/1/2015High Fidelity Restriction Enzyme for Cloning Prabhakaran KumarI used this EcoRI-HF restriction enzyme for one way (Single enzyme digestion) cloning of my gene of interest in to plasmid vector. Subsequently I used this enzyme for colony screening also. This ... Read Review

EcoRI-HF

EcoRI-HF from New England Biolabs

Description

Sequence

New England Biolabs

Citations (288)

Reviews

EcoRI-HF from New England Biolabs

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