EcoP15I from New England Biolabs

Product Specs
ItemEcoP15I
CompanyNew England Biolabs
Price $75.00 Inquire
Catalog NumberR0646S
Quantity500 units
Concentration10,000 units/ml
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

CAGCAG(ACGT)25/27

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) The insertion of an ATTTC repeat in an Alu element hyperactivates a neurodevelopmental enhancer in spinocerebellar ataxia type 37Cell ReportsApril 1, 2026Joana R. Loureiro, Ana F. Castro, Ana S. Figueiredo, Ana Eufrásio, Ashutosh Dhingra, Mafalda Galhardo, Hugo Marcelino, Catarina C. Rodrigues, Paula Sampaio, Maria Azevedo, et al.synthesis was performed to generate double-stranded cDNA. This cDNA was then digested with EcoP15I (#R0646S, NEB) to generate short tags, that were ligated to adapters and amplified by PCR, purified and sequenced

(2) Polysome-CAGE of TCL1-driven chronic lymphocytic leukemia revealed multiple N-terminally altered epigenetic regulators and a translation stress signatureeLifeAugust 8, 2022Ogran A, Havkin-Solomon T, Becker-Herman S, David K, Shachar I, Dikstein R.to the 5′ ends of the original mRNA. 5′ linkers harboring barcodes sequences and EcoP15I (NEB, cat. R0646S) recognition site were ligated, and second-strand synthesis was performed. Then, fragments of the first

(3) m6Am-seq reveals the dynamic m6Am methylation in the human transcriptomeNature CommunicationsDecember 1, 2021Hanxiao Sun, Kai Li, Xiaoting Zhang, Jun’e Liu, Meiling Zhang, Haowei Meng, Chengqi Yisecond-strand cDNA was synthesized using the biotin-modified primer. The cDNA was digested with EcoP15I (NEB, R0646S) and the 3′ linker was ligated to the 3′ end of cDNA. The cDNA was performed PCR amplification. The

(4) An improved method of constructing degradome library suitable for sequencing using Illumina platformPlant MethodsDecember 1, 2019Yong-Fang Li, Miao Zhao, Menglei Wang, Junqiang Guo, Li Wang, Jie Ji, Zongbo Qiu, Yun Zheng, Ramanjulu Sunkarligase (ThermoFisher, Cat. No. 15224-017)T4 RNA ligase (NEB, Cat. No. M0204S)EcoP15I (NEB, Cat. No. R0646S)Platinum® Taq DNA polymerase high fidelity (ThermoFisher, Cat. No. 11304011)20 bp DNA ladder (Takara,

(5) CAGE (Cap Analysis of Gene Expression): A Protocol for the Detection of Promoter and Transcriptional NetworksMethods in Molecular BiologyJune 4, 2014Hazuki Takahashi, Sachiko Kato, Mitsuyoshi Murata, Piero CarninciTaq (TAKARA, Cat# RR002A, 125 U)Antarctic Phosphatase (NEB, Cat# M0289L, 5000 U)EcoP15I (NEB, Cat# R0646S, 500 U)Sinefungin (Calbiochem-Novabiochem international, Cat# 567051, 2 mg)Phusion™ High-Fidelity DNA

(6) Type III restriction endonucleases are heterotrimeric: comprising one helicase–nuclease subunit and a dimeric methyltransferase that binds only one specific DNANucleic Acids ResearchApril 1, 2014Annika Butterer, Christian Pernstich, Rachel M. Smith, Frank Sobott, Mark D. Szczelkun, Júlia Tóthtypically yielded 10–15 mg/l of culture. The commercially available EcoP15I was from NEB (catalogue no: R0646S) supplied as a 10 000 U/ml solution in 10 mM Tris–HCl pH 7.4, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 200

(7) Microarray and Degradome Sequencing Reveal MicroRNA Differential Expression Profiles and Their Targets in Pinellia pedatisectaPLoS ONESeptember 25, 2013Miao Dong, Dongfeng Yang, Qiulei Lang, Wei Zhou, Shaowei Xu, Tao Xuto a chimeric 5′ RNA adapter (5′-GUUCAGAGUUCUACAGUCCGACGAUCCAGCAG-3′) containing a 3′ Ecop 15I (NEB, R0646S) recognition site using T4 RNA ligase (Promega, M1051). Reverse transcription was carried out to generate

(8) 5’ end-centered expression profiling using Cap-analysis gene expression (CAGE) and next-generation sequencingNature ProtocolsMarch 1, 2012Hazuki Takahashi, Timo Lassmann, Mitsuyoshi Murata, Piero Carnincicat. no. RR002A, 125 U)·Antarctic Phosphatase (NEB, cat. no. M0289L, 5000 U)·EcoP15I (NEB, cat. no. R0646S, 500 U)·Sinefungin (Calbiochem-Novabiochem international, cat. no. 567051, 2 mg)·Phusion™ High-Fidelity

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EcoP15I

EcoP15I from New England Biolabs

Description

Sequence

New England Biolabs

Citations (8)

Reviews

EcoP15I from New England Biolabs

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