DTT, Molecular Grade from Promega

Product Specs
ItemDTT, Molecular Grade
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberP1171
Quantity100microl

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Technical and biological sources of noise confound multiplexed enhancer AAV screeningNat CommunApril 28, 2026Avery C. Hunker, John K. Mich, Naz Taskin, Amy Torkelson, Trangthanh Cardenas, Jean-Benoît Lalanne, Joseph T. Mahoney, Darren Bertagnolli, Anish Bhaswanth Chakka, Rushil Chakrabarty, et al.Dounces were placed on ice and filled with 1.5 mL of Homogenization Buffer containing 0.1 mM DTT (Promega P1171), 1x Protease Inhibitor Cocktail (Promega G6521), 0.2 U/µL RNasin Plus RNase Inhibitor (Promega N2615),

(2) Evolution of neurohormone function revealed by actions of kisspeptin-type peptides in an echinodermBMC BiologyFebruary 18, 2026Tabinda Islam, Luis A. Yañez-Guerra, Dean C. Semmens, Riley T. Beskeen, Michaela Egertová, Maurice R. ElphickNo. 11277073910), transcription buffer (NEB, Cat. No. M0251S), 5-mM dithiothreitol (Promega, Cat. No. P1171), 1 U/μl placental ribonuclease inhibitor (NEB, Cat. No. M0307S), and 5 U/μl of SP6 RNA polymerase (NEB,

(3) State-specific enhancer landscapes govern microglial plasticityImmunityFebruary 10, 2026Nicole Hamagami, Dvita Kapadia, Kia M. Barclay, Yanlong Huang, Nora Abduljawad, Zuolin Cheng, Liam McLaughlin, Darsh Singhania, Xukai Ding, Jin Yang, et al.,Thermo Fisher Scientific,Cat#15630106 SMARTScribe™ Reverse Transcriptase,Clontech,Cat#639538 "DTT, Molecular Grade",Promega,Cat#P1171 1M Betaine,Sigma-Aldrich,Cat#B0300-5VL Lambda Exonuclease,New England BioLabs,Cat#M0262S 10mM dNTP

(4) Long-read RNA sequencing of transposable elements from single cells using CELLO-seqNature ProtocolsJuly 16, 2025Sophie A. Marlow, Lauryn A. Deaville, Rebecca V. Berrenscauses serious eye damage. Handle it using appropriate safety equipment.-DTT, Molecular Grade (Promega: P1171)CAUTION: DTT is toxic if swallowed. DTT causes skin corrosion and irritation as well as eye irritation

(5) Protocol for in vivo CRISPR knockout screening of autophagy genes to identify breast cancer metastasis suppressorsSTAR ProtocApril 30, 2025Mingang Hao, Peixin Lu, Jun-Lin Guanand recombinant proteins" ,, Polyethylenimine HCl MAX,Polysciences,Cat# 24765-1 DTT,Promega,Cat# P1171 ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, ZymoPURE

(6) Microglial plasticity governed by state-specific enhancer landscapesbioRxivFebruary 1, 2025Nicole Hamagami, Dvita Kapadia, Nora Abduljawad, Zuolin Cheng, Liam McLaughlin, Darsh Singhania, Kia M. Barclay, Jin Yang, Zhixin Sun, Peter Bayguinov, et al.,Thermo Fisher Scientific,Cat#15630106 SMARTScribeTM Reverse Transcriptase,Clontech,Cat#639538 "DTT, Molecular Grade",Promega,Cat#P1171 1M Betaine,Sigma-Aldrich,Cat#B0300–5VL Lambda Exonuclease,New England BioLabs,Cat#M0262S 10mM dNTP

(7) Proteomic profiling of kidney biopsies in nephrotic syndromeWellcome Open ResearchDecember 24, 2024Emily Williams, Maryline Fresquet, Anna S Li, Craig Lawless, David Knight, Elizabeth Colby, Judy Watson, Gavin I Welsh, Moin A Saleem, Rachel Lennon(Covaris LE220-plus focused ultrasonicator). Samples were reduced using 5mM dithiothreitol (DTT; Promega #P1171) for 10 minutes at 60°C, then alkylated with 15mM iodoacetamide (SLS, #I1149-5G) for 30 minutes (in

(8) A Vulnerable Subtype of Dopaminergic Neurons Drives Early Motor Deficits in Parkinson's DiseasebioRxivDecember 23, 2024Akira Fushiki, David Ng, Zachary R. Lewis, Archana Yadav, Tatiana Saraiva, Luke A. Hammand, Christoph Wirblich, Bosiljka Tasic, Vilas Menon, Joaquim Alves da da Silva, et al.inhibitor (Promega G6521), 0.5% RNasin Plus RNase inhibitor (Promega N2615) and 0.1mM DTT (Promega P1171). Tissues were placed into a 2ml dounce homogenizer (Sigma D8938) and homogenized using 10 strokes of

(9) Protocol for developing Pseudomonas aeruginosa type III secretion system-neutralizing monoclonal antibodies from human B cellsSTAR ProtocolsDecember 20, 2024Alexandra Albus, Christoph Kreer, Florian Klein, Jan Rybniker, Alexander Simonismix,Thermo Fisher Scientific,Cat.#R1122 DPBS,Thermo Fisher Scientific,Cat.# 14190250 DTT,Promega,Cat.#P1171 EcoRI-HF,NEB,Cat.#R3101 EDTA,Thermo Fisher Scientific,Cat.# AM9260G ELISA coating buffer,BioLegend,Cat.#

(10) Single-cell transcriptomics of vomeronasal neuroepithelium reveals a differential endoplasmic reticulum environment amongst neuronal subtypeseLifeDecember 13, 2024G V S Devakinandan, Mark Terasaki, Adish Danibuffer,Promega,P1181, "Peptide, recombinant protein",RNasin Plus,Promega,N2611, "Chemical compound, drug",100 mM DTT,Promega,P1171, "Chemical compound, drug",Diethyl pyrocarbonate,Sigma,40718–25 ML, "Chemical compound, drug",Akoya

(11) Modeling Glioma Intratumoral Heterogeneity with Primary Human Neural Stem and Progenitor CellsbioRxivOctober 25, 2024Daniel Gao, Daniel Dan Liu, Anna E. Eastman, Nicole L. Womack, Benjamin F. Ohene-Gambill, Michelle Baez, Irving L. Weissman(Thermo Fisher Scientific, AM9760G), 2.5 mM MgCl2 (Thermo Fisher Scientific, AM9530G), 8 mM dithiothreitol (DTT) (Promega, P1171), 0.5 U/μL RNase inhibitor (Clontech, 2313B), 1 mM GTP (Thermo Fisher Scientific, R0461), 2 μM TSO (Integrated

(12) Segregated basal ganglia output pathways correspond to genetically divergent neuronal subclassesbioRxivSeptember 22, 2024Alana I. Mendelsohn, Laudan Nikoobakht, Jay B. Bikoff, Rui M. CostaTris-HCl, 250 mM sucrose, 25 mM KCl, 5mM MgCl2, 10mM Tris-HCl ), 3.5 μl of 100 mM DTT stock (Promega P1171), 70 μl of 50X Protease inhibitor cocktail (Promega G6521), 17.5 μl of 40 U/μl RNAsin Plus (Promega

(13) Protocol to dissect and dissociate the mouse brainstem for single-cell RNA-seq applicationsSTAR ProtocMarch 15, 2024Wiktor S. Phillips, Naify Ramadan, Athina Samara, Eric Herleniuspyruvate,Merck/Sigma-Aldrich,E47808 Lyophilized bovine serum albumin,Merck/Sigma-Aldrich,10735078001 Dithiothreitol (100 mM),Promega,P1171 D-glucose,VWR,97061-166 Glycerol,VWR,4388.320 HEPES acid,Fischer Scientific,BP310-100 Hoechst 33342,Thermo

(14) Cell type-specific gene expression dynamics during human brain maturationbioRxivOctober 6, 2023Christina Steyn, Ruvimbo Mishi, Stephanie Fillmore, Matthijs B. Verhoog, Jessica More, Ursula K. Rohlwink, Roger Melvill, James Butler, Johannes M. N. Enslin, Muazzam Jacobs, et al.NUC101] or Nuclei PURE Lysis buffer [Sigma-Aldrich, NUC201] with 1 mM dithiothreitol [DTT, Promega, P1171, US] and 0.1% Triton X-100 [Sigma-Aldrich, NUC201–1KT, US]). Homogenisation was done 20 times with the

(15) KRAS(G12D) drives lepidic adenocarcinoma through stem-cell reprogrammingNatureJuly 1, 2023Juul NH, Yoon JK, Martinez MC, Rishi N, Kazadaeva YI, Morri M, Neff NF, Trope WL, Shrager JB, Sinha R, et al.RNase inhibitor (Takara, cat. #2313B), First Strand Buffer, 8 mM dithiothreitol (DTT) (Promega, cat. #P1171), 1M Betaine (), 2.5 mM MgCl2 (Thermo Fisher Scientific, cat. #AM9530G), and 2 μM TSO (Integrated DNA

(16) Histone modification analysis reveals common regulators of gene expression in liver and blood stage merozoites of Plasmodium parasitesEpigenetics & chromatinJune 15, 2023Reers AB, Bautista R, McLellan J, Morales B, Garza R, Bol S, Hanson KK, Bunnik EM.#BP366-500), 0.1 mM EDTA (Fisher #BP2482100), 0.1 mM EGTA (Boston BioProducts #BM1511), 1 mM DTT (Promega #P1171), 200 mM AEBSF (GoldBio #A-540–500), cOmplete protease inhibitor cocktail (1 tablet per 10 ml), PhosSTOP

(17) A systems biology approach uncovers novel disease mechanisms in age-related macular degenerationCell GenomicsJune 14, 2023Luz D. Orozco, Leah A. Owen, Jeffrey Hofmann, Amy D. Stockwell, Jianhua Tao, Susan Haller, Vineeth T. Mukundan, Christine Clarke, Jessica Lund, Akshayalakshmi Sridhar, et al.Reagent,Ventana,Cat# 760-2037 Discovery Purple,Ventana,Cat# 760-229 "DTT, molecular grade, 100uM",Promega,Cat# P1171 Hematoxylin II,Ventana,Cat# 790-2208 RNAlater,Ambion,Cat# AM7020 RNase inhibitor,Life Technologies,Cat#

(18) Protocol to determine the subcellular localization of protein interactions in murine keratinocytesSTAR protocolsMay 17, 2023Müller L.D-(+)-glucose solution,Sigma-Aldrich,Cat#G8769 Dimethyl sulfoxide (DMSO),Sigma-Aldrich,Cat#D2650 Dithiothreitol (DTT),Promega,Cat#P1171 "DMEM/Ham’s F12 (3.5:1.1) medium containing 50 μM CaCl2; customized formulation, w/o: Glucose, w/o:

(19) Human striatal glia differentially contribute to AD- and PD-specific neurodegenerationNature AgingMarch 30, 2023Jinbin Xu, Huifangjie L. Farsad, Yiran Hou, Kia Barclay, Ben Anthony Lopez, Shinnosuke Yamada, Ibrahim Olabayode Saliu, Yiming Shi, William C. Knight, Randall J. Bateman, et al.glass Dounce grinder in 4 ml ice-cold homogenization buffer (HB; consisting of 0.1 mM DTT (Promega, P1171), 1X Protease inhibitor cocktail (Promega, G6521), 0.2 U μl−1 RNasin Plus RNase Inhibitor (Promega,

(20) Coupling live-cell imaging and in situ isolation of the same single cell to profile the transient states of predicted drug-tolerant cellsSTAR ProtocolsSeptember 16, 2022Benjamin Bian, AgnÃ¨s Paquet, Marie-Jeanne Arguel, Mickael Meyer, Ludovic Peyre, Asma Chalabi, Marielle PÃ©rÃ©, Kevin Lebrigand, Rainer Waldmann, Pascal Barbry, et al.Scientific,15651859 Dimethyformamide,Sigma,227056 DMEM,Gibco,31966-021 DMEM without phenol red,Gibco,21063-029 dNTP,Promega,U1511 DTT,Promega,P1171 Dynabeads® MyOneTM Streptavidin C1 beads,Life Technologies,65001 EDTA,Invitrogen,15575-038 EtOH,Sigma,51976 FACS

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