DTT, Molecular Grade (Dry Powder) from Promega

Product Specs
ItemDTT, Molecular Grade (Dry Powder)
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberV3155
Quantity25g

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Breaking the cancer code: a novel DNA minicircle to disable STAT3 in ovarian cancer cells SKOV3Frontiers in PharmacologySeptember 15, 2025Adina-Gabriela Vasilescu, Andrei-Mihai Vasilescu, Livia Elena Sima, Natalia Baran, Ștefan-Eugen Szedlacsekcool down to room temperature (RT, 25 °C) to facilitate annealing. Subsequently, DTT (Promega; Cat no V3155) was added to a final concentration of 10 mM, ATP (NEB; Cat no P0756L) to a final concentration of 100

(2) Biophysical, Molecular and Proteomic Profiling of Human Retinal Organoid-Derived ExosomesPharmaceutical researchApril 1, 2023Arthur P, Kandoi S, Sun L, Kalvala A, Kutlehria S, Bhattacharya S, Kulkarni T, Nimma R, Li Y, Lamba DA, et al.were lysed with 2X SDS lysis buffer (10% SDS, 100 mM TEAB pH 8.5), reduced with 20 mM dithiothreitol (V3155, Promega, USA) at 95°C for 10 min and alkylated with 40 mM iodoacetamide (RPN6302V, Amresco, USA) at

(3) The Investigation of Protein Profile and Meat Quality in Bovine Longissimus thoracic Frozen under Different Temperatures by Data-Independent Acquisition (DIA) StrategyFoodsJune 17, 2022Xia Li, Shuyi Qian, Feng Huang, Kaimin Li, Yu Song, Jiqian Liu, Yujie Guo, Chunhui Zhang, Christophe BleckermM TEAB solution (this step was repeated two times). Each sample was digested with trypsin (cat. no. V3155, Promega, Madison, WI, USA) in a 1:50 ratio and incubated overnight at 37 °C. The desalted peptide mixture

(4) Capillary Nano-immunoassay for Quantification of Proteins from CD138-purified Myeloma CellsBio-protocolJune 20, 2019Misiewicz-Krzeminska I, Isidro I, Gutiérrez NC.(Santa Cruz Biotechnology, catalog number: sc29130) 22. Deionized water 23. DTT (Promega, catalog number: V3155), store at -20 °C 24. Ethanol 70% (see Recipes) 25. 5 M NaCl (see Recipes) 26. 0.2 M NaOH (see Recipes) 27.

(5) H2 drives metabolic rearrangements in gas-fermenting Clostridium autoethanogenumBiotechnology for BiofuelsDecember 1, 2018Kaspar Valgepea et al.resuspended in 500 µL of lysis buffer (pH 7.6) containing 2% (w/v) SDS (L4390; Sigma-Aldrich), 0.1 M DTT (V3155; Promega), 0.1 M Trizma® base (T1503; Sigma-Aldrich), and vortexed. The cell suspension was transferred

(6) Guanine Nucleotide Exchange Assay Using Fluorescent MANT-GDPBIO-PROTOCOLApril 5, 2018Tomoharu Kanie, Peter K. Jackson(MgCl2·6H2O) (Sigma-Aldrich, catalog number: M2670) 42. Dithiothreitol (DTT) (Promega, catalog number: V3155) 43. Ethylenediaminetetraacetic acid (EDTA) (Fisher Scientific, catalog number: S311) 44. LB broth (Fisher Scientific

(7) Optimization of overexpression of a chaperone protein of steroid C25 dehydrogenase for biochemical and biophysical characterizationProtein Expression and PurificationJune 1, 2017Ewa Niedzialkowska, Beata Mrugała, Agnieszka Rugor, Mateusz P. Czub, Anna Skotnicka, Julien J. H. Cotelesage, Graham N. George, Maciej Szaleniec, Wladek Minor, Krzysztof Lewińskicoenzyme A lithium salt,10 mM,Sigma H0261 ,,, Reducing agents,DL-Dithiothreitol (DTT),5 mM,Promega V3155 ,2-Mercaptoethanol,5 mM,Aldrich M6250 ,Tris(2-carboxyethyl)phosphine hydrochloride (TCEP),5 mM,Aldrich

(8) In Primary Aldosteronism, Mineralocorticoids Influence Exosomal Sodium-Chloride Cotransporter AbundanceJournal of the American Society of NephrologyJanuary 1, 2017Martin J. Wolley, Aihua Wu, Shengxin Xu, Richard D. Gordon, Robert A. Fenton, Michael Stowasserwhole cells and debris, and the supernatant was then incubated with 1 ml 200 mg/ml dithiothreitol (V3155; Promega, Madison, WI) per 24-ml sample for 10 minutes at 37°C. The samples then underwent ultracentrifugation

(9) Integrated mass spectrometry–based analysis of plasma glycoproteins and their glycan modificationsNature ProtocolsMarch 5, 2011Hong Wang et al.guidelines.Trypsin (mass spectrometry grade; Promega, cat. no. V511A; store at 4 °C)DL-DTT (Promega, cat. no. V3155) (store at −20 °C)Acrylamide (Fluka, cat. no. 01696) (store at 4 °C, in dark)Octyl β-D-glucopyranoside

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