DraIII-HF from New England Biolabs

Product Specs
ItemDraIII-HF
CompanyNew England Biolabs
Price $75.00 Inquire
Catalog NumberR3510S
Quantity1000 units
Concentration20,000 units/ml
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

CACNNNGTG

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Human antibodies against West Nile and related orthoflavivirusesbioRxivApril 6, 2026Tomás Cervantes Cervantes Rincón, Tereza Frckova, Zaria I. Contejean, Jasmine Cantergiani, Kevin Groen, Benedetta Cena, Simone G. Moro, Filippo Bianchini, Luca Simonelli, David Jarrossay, et al.pWNVII-Rep-REN-IB, previously digested with MluI (New England Biolabs, R3198S) and DraIII (New England Biolabs, R3510S), resulting in plasmid pWNVII-Rep-NanoLuc. The plasmid expressing the WNV CprME was previously reported

(2) RNA polymerase II is a polar roadblock to a progressing DNA forkNature CommunicationsSeptember 29, 2025Taryn M. Kay, James T. Inman, Lucyna Lubkowska, Tung T. Le, Jin Qian, Porter M. Hall, Sahil Batra, Dirk Remus, Dong Wang, Mikhail Kashlev, et al.from pBR322 (NEB N3033S) and enzymatically digested with both PpuMI (NEB R0506) and DraIII-HF (NEB R3510S) to generate a 0.65 kb strand. The downstream segment was amplified from pRL574 and digested with BstXI

(3) A nucleosome switch primes hepatitis B virus infectionCellApril 17, 2025Nicholas A. Prescott, Tracy Biaco, Andrés Mansisidor, Yaron Bram, Justin Rendleman, Sarah C. Faulkner, Abigail A. Lemmon, Christine Lim, Rachel Tiersky, Eralda Salataj, et al.HY-114268 CBL0137 HCl,Selleckchem,Cat# S8483 "Collagen I, rat tail",Gibco,Cat# A1048301 DraIII,NEB,Cat# R3510S "DNase I, RNase-free",Thermo Scientific,Cat# EN0521 Doxorubicin HCl,Thermo Scientific Chemicals,Cat#

(4) Control of DNA replication in vitro using a reversible replication barrierNature ProtocolsJuly 1, 2024Emma J. Vontalge, Tamar Kavlashvili, Steven N. Dahmen, Matthew T. Cranford, James M. Dewar(Thermo Fisher Scientific, cat. no. FLA322500) XhoI (NEB, cat. no. R0146S) DraIII-HF (NEB, cat. no. R3510S) Loading dye: Bromocresol green (Fisher Chemical, cat. no. B383–5) Loading dye: Xylene cyanol (Thermo Scientific

(5) Single-molecule RNA sizing enables quantitative analysis of alternative transcription terminationNature CommunicationsFebruary 24, 2024Gerardo Patiño-Guillén, Jovan Pešović, Marko Panić, Dušanka Savić-Pavićević, Filip Bošković, Ulrich Felix Keysercharacterized using nanopore sensing. Circular DNA plasmid digestion DraIII-HF (New England Biolabs (NEB), R3510S) was used to linearize the circular DNA plasmid (sequence in Supplementary Table 1) following the manufacturer’s

(6) Age-dependent loss of cohesion protection in human oocytesCurrent biology : CBJanuary 8, 2024Mihalas BP, Pieper GH, Aboelenain M, Munro L, Srsen V, Currie CE, Kelly DA, Hartshorne GM, Telfer EE, McAinsh AD, et al.above) and Addgene, #20972,53 respectively. H2B-mCherry was linearized by DraIII (New England Biolabs, R3510S). After linearization, the digests were purified using Promega PCR Purification kit (Wizard® SV Gel and PCR Clean-Up System

(7) Structural and functional basis of the universal transcription factor NusG pro-pausing activity in Mycobacterium tuberculosisMolecular CellMay 1, 2023Madeleine Delbeau, Expery O. Omollo, Ruby Froom, Steven Koh, Rachel A. Mooney, Mirjana Lilic, Joshua J. Brewer, Jeremy Rock, Seth A. Darst, Elizabeth A. Campbell, et al.the relevant species. The amplified fragments were then cloned into DraIII-HF (New England Biolabs, #R3510S)–digested plRL91. Identification of desired clones was confirmed by whole-plasmid sequencing with Plasmidsaurus.

(8) The essential M. tuberculosis Clp protease is functionally asymmetric in vivoScience advancesMay 6, 2022d'Andrea FB, Poulton NC, Froom R, Tam K, Campbell EA, Rock JM.amplified, CRISPRi-resistant allele fragments were then cloned into HF–Dra III (New England Biolabs, #R3510S)–digested plRL91 or HpaI (New England Biolabs, #R0105S)–digested plRL125 backbones by Gibson assembly

(9) Serine ADP-ribosylation marks nucleosomes for ALC1-dependent chromatin remodelingeLifeDecember 7, 2021Jugal Mohapatra, Kyuto Tashiro, Ryan L Beckner, Jorge Sierra, Jessica A Kilgore, Noelle S Williams, Glen Liszczak,Phusion High-Fidelity DNA Polymerase,NEB,M0530L, "Chemical compound, drug",PstI enzyme,NEB,R0140M, "Chemical compound, drug",DraIII enzyme,NEB,R3510S, "Chemical compound, drug",T4 Polynucleotide Kinase,NEB,M0201S, "Chemical compound, drug",T4 DNA Ligase,NEB,M0202L, "Chemical

(10) Serine ADP-ribosylation marks nucleosomes for ALC1-dependent chromatin remodelingElifeDecember 7, 2021Jugal Mohapatra, Kyuto Tashiro, Ryan L Beckner, Jorge Sierra, Jessica A Kilgore, Noelle S Williams, Glen Liszczak,Phusion High-Fidelity DNA Polymerase,NEB,M0530L, "Chemical compound, drug",PstI enzyme,NEB,R0140M, "Chemical compound, drug",DraIII enzyme,NEB,R3510S, "Chemical compound, drug",T4 Polynucleotide Kinase,NEB,M0201S, "Chemical compound, drug",T4 DNA Ligase,NEB,M0202L, "Chemical

(11) Mapping enzyme catalysis with metabolic biosensingNature CommunicationsDecember 1, 2021Linfeng Xu et al.restriction enzyme digestion and ligation (Supplementary Fig. 1). The pTEF-2PS plasmid is linearized by DraIII and AflII (R3510S and R0520S, New England Biolabs) digestion. The library amplicons are cloned into linearized pTEF-2PS

(12) Cas9 interrogates DNA in discrete steps modulated by mismatches and supercoilingProceedings of the National Academy of SciencesMarch 17, 2020Ivan E. Ivanov, Addison V. Wright, Joshua C. Cofsky, Kevin D. Palacio Aris, Jennifer A. Doudna, Zev BryantLinear and nicked versions of the template were generated by treatment with DraIII (New England Biolabs, R3510S) and Nb.BsrDI (New England Biolabs, R0648S), respectively. The SPD640 template used in time course experiments

(13) High-resolution and high-accuracy topographic and transcriptional maps of the nucleosome barriereLifeJuly 31, 2019Zhijie Chen, Ronen Gabizon, Aidan I Brown, Antony Lee, Aixin Song, César Díaz-Celis, Craig D Kaplan, Elena F Koslover, Tingting Yao, Carlos Bustamantekit Phusion high-fidelity DNA polymerase NEB Cat# M0530S Commercial assay or kit DraIII-HF NEB Cat# R3510S Commercial assay or kit BsaI-HF NEB Cat# R3535L Commercial assay or kit BglI NEB Cat# R0143S Commercial assay

(14) Quantification of cytosolic plasmid DNA degradation using high-throughput sequencing: implications for gene deliveryThe Journal of Gene MedicineMay 28, 2014Rahul Rattan, Anna U. Bielinska, Mark M. Banaszak Holl0816– 015). Three restriction endonucleases, SacI (catalogue number R3156S), DraIII (catalogue number R3510S) and StuI (catalogue number R0187S), were obtained from New England BioLabs, Inc. (Beverly, MA, USA).

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DraIII-HF

DraIII-HF from New England Biolabs

Description

Sequence

New England Biolabs

Citations (14)

Reviews

DraIII-HF from New England Biolabs

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