DraI from New England Biolabs

Product Specs
ItemDraI
CompanyNew England Biolabs
Price $294.00 Inquire
Catalog NumberR0129L
Concentration20 u/µL
Quantity10000 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

TTT/AAA

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Fluorescence-Guided Spatial Mapping of p21-Expressing Senescent Cells in Aged Liver at Single-Cell ResolutionBiomoleculesApril 14, 2026Jer-En Hsu, Binsheng Wang, Yongha Hwang, Weiqiu Cheng, Qingyang Zhao, Yichen Si, Ming Xu, Hyun-Min Kang, Jun Hee Leewas washed with nuclease-free water three times initially, followed by overnight incubation of DraI (R0129, NEB, Ipswich, MA, USA) and CIAP (M0525, NEB) enzyme mixture at 37 °C. The flow cell was treated with

(2) Genetic Modification of Mice Using Prime EditingCurrent ProtocolsMarch 11, 2026Amr R. Salem, Xiaoling Xie, Susan H. Griffin, Lin Gan, Joseph M Mianoplasmid purification kit (Invitrogen, cat. no. A33073) 50% (v/v) glycerol solution DraI (NEB, cat. no. R0129S) Mass ruler DNA ladder (Thermo Fisher, cat. no. SM0403) RNAsecure reagent (Ambion, cat. no. AM7005) MEGAshortscript T7 transcription kit

(3) PRISM-Seq: An Ultra-sensitive Sequencing Approach For Mapping Lentiviral Integration SitesbioRxivFebruary 7, 2026V K Pal, M Canis, E Stone, N Board, K Lenart, M Fumagalli, C Kovacs, R B Jones, M Nussenzweig, T Hatziioannou, et al.using three blunt-end restriction enzymes - HpaI (NEB #R0105L), SspI (NEB #R3132L), and DraI (NEB #R0129L) - to fragment genomic DNA and facilitate subsequent ligation-based integration site analysis. Briefly,

(4) MagIC-Cryo-EM: Structural determination on magnetic beads for scarce macromolecules in heterogeneous samplesElifeMay 20, 2025Yasuhiro Arimura, Hide A Konishi, Hironori Funabikiprotein",HaeII,New England Biolabs,R0107S, "Peptide, recombinant protein",DraI,New England Biolabs,R0129S, "Peptide, recombinant protein",EcoRI,New England Biolabs,R3101S, "Peptide, recombinant protein",XbaI,New England Biolabs

(5) Clinical response to azacitidine in MDS is associated with distinct DNA methylation changes in HSPCsNat CommunMay 13, 2025Julie A. I. Thoms, Feng Yan, Henry R. Hampton, Sarah Davidson, Swapna Joshi, Jesslyn Saw, Chowdhury H. Sarowar, Xin Ying Lim, Andrea C. Nunez, Purvi M. Kakadia, et al.nM each of forward and reverse outer primers and FAM probe), 2U HpaII (NEB #R0171L), 1U DraI (NEB #R0129L), 0.5U Hot Start Taq DNA Polymerase (NEB #M0495L)) were cycled at 37 °C for 15 min; 90 °C for 5 s; 95

(6) Identification of microproteins with transactivation activity by polyalanine motif selection†RSC Chemical BiologyMay 8, 2025Archita Agrawal, Alan Saghatelianbackbone vector. Restriction digestion for GAL4-ERα was performed using PvuI (NEB #R0150S) and DraI (NEB #R0129S) to remove the ERα-encoding fragment, and the desired vector product was gel purified for use as a backbone

(7) Variant ribosomal DNA is essential for female differentiation in zebrafishPhilos Trans R Soc Lond B Biol SciMarch 6, 2025Tim V. Moser, Donna M. Bond, Timothy A. Hore(New England Biolabs, R3733). The resulting pDR274:GFPsgRNA vector was digested with DraI (New England Biolabs, R0129) and used as an in vitro transcription template. All sgRNA templates were in vitro transcribed using

(8) Protocol for high-resolution 3D spatial transcriptomics using Open-STSTAR ProtocolsMarch 1, 2025Marie Schott, Daniel León-Periñán, Elena Splendiani, Elisabetta Ferretti, Giuseppe Macino, Nikos Karaiskos, Nikolaus Rajewskypeptides, and recombinant proteins" ,, RNase AWAY,Thermo Fisher Scientific,Cat#7003 Dra I enzyme,NEB,Cat#R0129 Alkaline phosphatase calf intestinal (CIAP) enzyme,Promega,Cat#M1821 Exonuclease I enzyme,NEB,Cat#M0293 "NaOH

(9) Seq-Scope: repurposing Illumina sequencing flow cells for high-resolution spatial transcriptomicsNature ProtocolsMarch 1, 2025Yongsung Kim, Weiqiu Cheng, Chun-Seok Cho, Yongha Hwang, Yichen Si, Anna Park, Mitchell Schrank, Jer-En Hsu, Angelo Anacleto, Jingyue Xi, et al.Gibco/Thermo Fisher, cat. no. 14190-144) Dra I restriction enzyme (New England Biolabs, cat. no. R0129) 0.5 M EDTA pH 8.0 (Invitrogen™/Thermo Fisher, Carlsbad, CA, USA; cat. no. AM9261) Eosin Y solution

(10) Lentiviral vectors for precise expression to treat X-linked lymphoproliferative diseaseMolecular therapy. Methods & clinical developmentDecember 12, 2024Ayoub PG, Gensheimer J, Lathrop L, Juett C, Quintos J, Tam K, Reid J, Ma F, Tam C, McAuley GE, et al.1863010; Bio-Rad, Hercules, CA), 400 nmol/L primers, and 100 nmol/L probe for each set, 40 U DraI (R0129S; New England Biolabs, Ipswich, MA) and 30–100 g of the gDNA were prepared and incubated at 37°C for

(11) Stabilization of a single-stranded DNA of adeno-associated virus by inverted terminal repeatsScientific ReportsNovember 12, 2024Yuzhe Yuan, Kiyoko Higashiyama, Emi Ito-Kudo, Kyoko Masumi-Koizumi, Keisuke Yusa, Kazuhisa Uchidaenzymes were purchased from New England Biolabs (USA). After restriction enzyme treatment, DraI (cat. #R0129), HindIII (cat. #R3104), MlyI (cat. #R0610), MluI (cat. # R3198), PstI (cat. #R3140), PvuII (cat. #R3151),

(12) High-Resolution Spatial Transcriptomic Atlas of Mouse Soleus Muscle: Unveiling Single Cell and Subcellular Heterogeneity in Health and DenervationbioRxivFebruary 29, 2024Jer-En Hsu, Lloyd Ruiz, Yongha Hwang, Steve Guzman, Chun-Seok Cho, Weiqiu Cheng, Yichen Si, Peter Macpherson, Mitchell Schrank, Goo Jun, et al.Seq-Scope array. The flow cell was washed with nuclease-free water three times and incubated with DraI (R0129, NEB) and CIAP (M0525, NEB) enzyme mixture in 37 °C overnight to expose oligo-dT end. The flow cell

(13) Grk7 but not Grk1 undergoes cAMP-dependent phosphorylation in zebrafish cone photoreceptors and mediates cone photoresponse recovery to elevated cAMPThe Journal of biological chemistryDecember 1, 2022Chrispell JD, Xiong Y, Weiss ER.Sanger sequencing (Genewiz), plasmid clones containing the correct insert were linearized with DraI (#R0129S, New England Biolabs) and sgRNA was synthesized in vitro using a MAXIscript T7 Transcription Kit (#AM1312,

(14) A transcriptional roadblock protects yeast centromeresNucleic Acids ResearchAugust 12, 2022Sabrine Hedouin, Glennis A Logsdon, Jason G Underwood, Sue Biggins7.6, 0.1% NP-40, 175 mM K-glutamate and 15% glycerol) supplemented with 0.1% BSA, 10 U of DraI (NEB, #R0129S) and 2.5 U of SspI (NEB, #R0132S) and incubated at 30°C for 15 min to allow the cleavage of kinetochore-unbound

(15) Structural basis of RNA conformational switching in the transcriptional regulator 7SK RNPMolecular CellMay 1, 2022Yuan Yang, Shiheng Liu, Sylvain Egloff, Catherine D. Eichhorn, Tanya Hadjian, James Zhen, Tamás Kiss, Z. Hong Zhou, Juli Feigon2005",N/A Spermidine,Sigma-Aldrich,Cat. # S2626 BamHI-HF restriction enzyme,NEB,Cat # R3136M DraI restriction enzyme,NEB,Cat # R0129S T4 Polynucleotide Kinase,NEB,Cat # M0201L Dulbecco’s Modified Eagle Medium (DMEM),"GIBCO, Invitrogen",Cat

(16) The Australian dingo is an early offshoot of modern breed dogsScience AdvancesApril 22, 2022Matt A. Field et al.Supermix (Bio-Rad), 1 μl of each 20× primer/probe, 1 μl of Dra I restriction enzyme (New England Biolabs #R0129S), 5 μl of DNA template (4 ng/μl), and 2 μl of ddH2O. Copy number data were rounded to the nearest whole

(17) Chromosome-length genome assembly and structural variations of the primal Basenji dog (Canis lupus familiaris) genomeBMC GenomicsDecember 1, 2021Richard J. Edwards et al.Supermix (Bio-rad), 1 μl of each 20x primer/probe, 1 μl of DraI restriction enzyme (New England BioLabs #R0129S), 5 μl of DNA template (4 ng/μl) and 2 μl ddH2O. Primer sequence for Amy2B: forward 5′-CCAAACCTGGACGGACATCT-3′

(18) Microscopic examination of spatial transcriptome using Seq-ScopeCellJune 24, 2021Cho CS, Xi J, Si Y, Park SR, Hsu JE, Kim M, Jun G, Kang HM, Lee JH.nuclease-free water 3 times. Then, the flow cell was treated with DraI enzyme cocktail (1U DraI enzyme (#R0129, NEB) in 1X CutSmart buffer) in 37 °C overnight to completely cut out the P5 sequence and expose oligo-dT.

(19) DNA length tunes the fluidity of DNA-based condensatesBiophysical journalApril 6, 2021Muzzopappa F, Hertzog M, Erdel F.(NEB, R3136), and KpnI (NEB, R0142); or 4) AciI (NEB, R0551), HindIII (NEB, R0104), and DraI (NEB, R0129). The 1 kb and 5 kb fragments were generated by PCR using the oligonucleotides LambdaFW and Lambda1_rv/Lambda5_rv

(20) Topological stress is responsible for the detrimental outcomes of head-on replication-transcription conflictsCell reportsMarch 2, 2021Lang KS, Merrikh H.and recombinant proteins",, EcoRV-HF,NEB,R0195 HindIII-HF,NEB,R0104 EcoRI-HF,NEB,R0101 DraI,NEB,R0129 RNase H,NEB,M0297 Protein A Sepharose,GE,GE17-0780-01 Critical commercial assays,, Nextera XT DNA Library Preparation

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DraI

DraI from New England Biolabs

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DraI from New England Biolabs

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