2X Rapid Ligation Buffer from Promega

Product Specs
Item2X Rapid Ligation Buffer
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberC6711
Quantity1.5ml
Molecular Weight~80kDa
TypeBuffer

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Comprehensive benchmarking of genome editing quantification methods for plant applicationsiScienceApril 29, 2025Zheng Gong, Yan Zhang, Di Xia, Sohye Yoon, Peter Alexander Crisp, José Ramón BotellaMix,New England Biolabs,M0492S GoTaq Green PCR Master Mix,Promega,M7122 Rapid ligation buffer,Promega,C6711 T4 DNA ligase,Promega,M1801 T7 Endonuclease 1,New England Biolabs,M0302S Exonuclease 1,New England Biolabs

(2) Evolutionary Co-Option of an Ancestral Cloacal Regulatory Landscape During the Emergence of Digits and GenitalsbioRxivMarch 30, 2024Aurélie Hintermann, Christopher Chase Bolt, M. Brent Brent Hawkins, Guillaume Valentin, Lucille Lopez-Delisle, Sandra Gitto, Paula Barrera Gómez, Bénédicte Mascrez, Thomas A. Mansour, Tetsuya Nakamura, et al.X52326.1)73 were digested with XhoI or SalI and ligated together with Promega 2× Rapid Ligation kit (#C6711). Sanger sequencing confirmed the correct sequences were inserted upstream of the promoter. Maxipreps

(3) Srd5a1 is Differentially Regulated and Methylated During Prepubertal Development in the Ovary and HypothalamusJournal of the Endocrine SocietyAugust 28, 2023Ben Bar-Sadeh, Lilach Pnueli, Sarai Keestra, Gillian R Bentley, Philippa Melamedexpress mCherry fluorescent protein, with T4 DNA ligase (M180B, Promega) in T4 Rapid Ligation Buffer X2 (C6711, Promega), for 60 minutes at room temperature. Cells were transfected with this plasmid as described

(4) Context-dependent enhancer function revealed by targeted inter-TAD relocationNature CommunicationsDecember 1, 2022Christopher Chase Bolt, Lucille Lopez-Delisle, Aurélie Hintermann, Bénédicte Mascrez, Antonella Rauseo, Guillaume Andrey, Denis Dubouleconstruct were digested with XhoI and HindIII and ligated together with Promega 2× Rapid Ligation kit (C6711) to produce pSK-II1-LoxP-LacZ construct. This vector (15 μg) was cut with XbaI and XhoI to release the

(5) Endogenous C-terminal Tagging by CRISPR/Cas9 in Trypanosoma cruziBio-protocolMay 20, 2017Lander N, Chiurillo MA, Vercesi AE, Docampo R.catalog number: M1801) (storage temperature: −20 °C) 2× rapid ligation buffer (Promega, catalog number: C6711) (storage temperature: −20 °C) LB broth (Sigma-Aldrich, catalog number: L3022) LB broth with agar

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