T4 DNA Ligase Reaction Buffer from New England Biolabs

Product Specs
ItemT4 DNA Ligase Reaction Buffer
CompanyNew England Biolabs
Price $27.00 Inquire
Catalog NumberB0202S
Quantity5 ml
ApplicationsDNA Manipulation
TypeBuffer
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Polyphosphate as a novel regulator of super-enhancer complexes: disruption of phase separation and gene expressionNucleic Acids ResMay 26, 2026Zhiyun Yang, Zongchao Jialigation assay, ~2 nM DNA, 0.96 µg/L YY1, 5 mM polyP700 or ddH2O, and 1× T4 DNA ligase buffer (NEB, #B0202S) were incubated in a buffer composed of 10 mM Tris–HCl (pH 7.5), 100 mM KCl, 10 µM ZnCl2, 1 mM DTT,

(2) An autochthonous CRISPR activation screening platform for characterizing tissue-specific oncogene selectionCell Reports MedicineMay 19, 2026Fredrik I. Thege, Amber Hoskins, Annaliese Kramer, Intisar Salim, Ashwath Seetharaman, Natalie Fowlkes, Kimal I. Rajapakshe, Anirban Maitra, Sonja M. WoermannP,Sigma,Cat# 11213857001 DNase I (bovine pancreas),Sigma,Cat# D5025-150KU 10X T4 ligase buffer,NEB,B0202S T4 PNK,NEB,M0201S T7 ligase,NEB,M0318S 2x StickTogether DNA Ligase Buffer,NEB,M0318S BsmBI-v2,NEB,R0739S KAPA HiFi HotStart ReadyMix

(3) Poliovirus 3Dpol polymerase region is essential for cleavage of poliovirus 3AB in vivoPLoS PathogMay 13, 2026Minetaro Aritausing a NucleoSpin Gel and PCR Clean-up kit (Takara Bio, #740609.250). T4 DNA ligase buffer (NEB, #B0202S) (3 μL) and T4 DNA ligase (NEB, #M0202L) (800 U, 2 μL) were added to the purified RT-PCR products (25

(4) A multiplex, prime editing framework for identifying drug resistance variants at scaleCell GenomicsMay 13, 2026Florence M.C. Abadie, Chase C. Suiter, Nahum T. Smith, Riza M. Daza, Mary C. Rominger, Phoebe Parrish, Troy A. McDiarmid, Jean-Benoît Lalanne, Beth Martin, Diego Calderon, et al.(New England Biolabs, Cat. No. M0202S) and 1 μL of 10X T4 DNA ligase reaction buffer (New England Biolabs, Cat. No. B0202S) in a final volume of 10 μL. 1 μL of the assembly reaction was transformed into 50 μL of stable competent

(5) Light-guided actin polymerization drives directed motility in protocellsbioRxivApril 23, 2026Hideaki T. Matsubayashi, Shiva Razavi, Yuhei O. Tahara, Oghosa H. Akenuwa, T. Willow Rock, Daichi Nakajima, Rina Otsuka-Yamaguchi, Hideki Nakamura, Daniel A. Kramer, Tomoaki Matsuura, et al.sequences)). To anneal oligo DNAs individually, 0.5 μM each DNAs were mixed in 1× T4 Ligase buffer (NEB, B0202S) and heated at 85°C for 10 min and cooled 5°C per 2 min down to 40°C. pGEX-2T, which was kindly gifted

(6) T-cell protrusions enable fast, localised initiation of chimeric antigen receptor signallingThe EMBO JournalApril 21, 2026Carmen Rodilla-Ramirez, Giorgia Carai, Eleanor Fox, Amin Zehtabian, Helen Adam, Katja Dallio, Pia Lazki-Hagenbach, Helge Ewers, Xiaolei Su, Francesca Bottanellideoxycholate,Sigma-Aldrich,D6750 Sodium orthovanadate (Na3VO4),Sigma-Aldrich,450243-5OG Sulphuric acid,Roth,9896.2 T4 ligase buffer,NEB,B0202S Tris-HCl,Roth,T845.2 Triton-X 100,Sigma-Aldrich,T8787 Tween20,Roth,9127.1 Software,Software,Software Affinity

(7) Phage homing endonuclease amplifies anti-defense genes to evade bacterial immunityNat CommunApril 20, 2026Kotaro Chihara, Aa Haeruman Azam, Artyom A. Egorov, Ilya Terenin, Masanori Hashino, Koichi Watashi, Kazuhiro Horiba, Vasili Hauryliuk, Kotaro Kigatemperature. Ligation reactions containing end-prepped DNA, annealed Adapter 1, T4 DNA ligase buffer (NEB, #B0202S), and NEB Quick Ligase (NEB, #M2200S) were incubated at 20 °C for 30 min and then at 16 °C overnight.

(8) Complementary constraints in germ and immune cells shape evolution of gene regulation and phenotypebioRxivMarch 31, 2026Kimberly N Griffin, Kira L Marshall, Gabriel A Russell, John Attanasio, Delaney B Farris, Haoming Yu, Eric Fagerberg, Nivedita R Iyer, Rebecca Lee, Kaelyn D Sumigray, et al.sgRNA oligos were phosphorylated and annealed using T4 PNK (NEB, M0201S) and T4 Ligation Buffer (NEB, B0202S). Oligos were diluted 1:100 and ligated with digested lentiCRISPR v2 plasmid using T4 Ligase (NEB, M0202S)

(9) A critical signaling role for diacylglycerol in phagocytosis of M. tuberculosisbioRxivMarch 26, 2026Alec Griffith, Michelle Garcia, Gaelen Guzman, Fikadu G. Tafesseusing T4 PNK (New England Biolabs, product M0236S) and T4 ligation buffer (New England Biolabs, product B0202S)49. The digested plasmid and annealed oligos were ligated overnight at room temperature in the presence

(10) RF-SIRF reveals a replication stress-specific epigenetic code by spatio-temporal mapping of reversed forksNature CommunicationsMarch 23, 2026Sunetra Roy, Morgan M. Fimreite, Yue Chen, Francesca Citron, Giulio Draetta, Katharina Schlacher(ab64226), Camptothecin (S1288) was from Selleck chemicals, T4 DNA ligase buffer from New England Biolabs (B0202S). Antibodies used for SIRF assays are as follows: mouse anti-biotin (BN-34, 1:100; Sigma-Aldrich), rabbit

(11) Chromatin reorganization drives overexpression of a Btaf1 variant underpinning hematopoietic agingNature CommunicationsMarch 18, 2026Le Zong, Bongsoo Park, Yaqiang Cao, Fei Ma, Ferda Tekin-Turhan, Wakako Kuribayashi, Keji Zhao, Isabel Beerman1.5 h with 1,000 rpm mixing. 450 μl of ligation master mix containing 60 μl of T4 DNA ligase buffer (B0202S, NEB), 47.5 μl of 10% Triton X-100, 3 μl of 20 mg/ml BSA (B9000S, NEB), 5 μl of 400 U/μl T4 DNA Ligase

(12) Synthetic circuits for cell ratio controlNatureMarch 18, 2026Bolin An, Tzu-Chieh Tang, Qian Zhang, Teng Wang, Yanyi Wang, Kesheng Gan, Kun Liu, Daniel L. Zhang, Yuzhu Liu, Yu Kui Pan, et al.cloning. Assembly reactions contained 6.5 μl DNA inserts, 1.0 μl backbone, 1.0 μl T4 ligase buffer (B0202S, NEB), 0.5 μl T4 DNA ligase (M0202L, NEB) and 1.0 μl restriction enzyme, adjusted to a total volume

(13) Multiplexed TrAEL-seq captures DNA replication dynamics in mammalian cellsNucleic Acids ResMarch 14, 2026Neesha Kara, Laura Biggins, Alex Whale, Kieron May, Vera Grinkevich, Paola Garran-Garcia, Jhanavi Srinivasan, Peter J Rugg-Gunn, Claudia Ribeiro de Almeida, Samantha J Walker, et al.reaction volume of 200 µl with 20 µl 100 pM/µl oligonucleotide and 20 µl 10× T4 DNA ligase buffer (NEB, B0202S) and heated at 95°C for 5 min. The reaction was removed from heat and left to cool to room temperature

(14) PEGASUS: Unlocking Polarity in Cell-Permeable Cyclic Peptides Using AI Models Built on Massively Parallel Biological AssaysJournal of Medicinal ChemistryMarch 12, 2026Cole Baker, Francis A. Acquah, Lakshmi G. Chivukula, Liping Wu, Laurence Philippe-Venec, Mostafa Abedi, Yujun Tao, Daniel Ramírez, Matthew D. McCoy, Brandon Moore, et al.purified mRNA was mixed with 20 pM of Puromycin linker (Bio-Synthesis) along with T4 ligation buffer (NEB, B0202S), annealed by heating to 90 °C, cooled to 25 °C, then cross-linked by UV light. The cross-linked product

(15) Holistic determination of ends of cfDNA moleculesCell GenomicsMarch 11, 2026Peiyong Jiang, Mary-Jane L. Ma, Rong Qiao, Yuwei Shi, Jing Liu, Qing Zhou, Wenlei Peng, W.K. Jacky Lam, Jinyue Bai, L.Y. Lois Choy, et al.Bioscience,CBS-UMB-96 T4 Polynucleotide Kinase,New England Biolabs,M0202L T4 DNA ligase buffer,New England Biolabs,B0202S T4 DNA ligase,New England Biolabs,M0202L SMRTbell® enzyme cleanup kit 2.0,Pacific Biosciences,101-932-600 Qubit™

(16) Genetic Modification of Mice Using Prime EditingCurrent ProtocolsMarch 11, 2026Amr R. Salem, Xiaoling Xie, Susan H. Griffin, Lin Gan, Joseph M Miano2006) T4 polynucleotide kinase (PNK) (NEB, cat. no. M0201L) T4 ligase buffer (ATP source) (NEB, cat. no. B0202S) T4 DNA ligase (NEB, cat. no. M0202S) Competent cells (Invitrogen, cat. no. C404006) Agar plates with

(17) Massively parallel reporter assay for mapping gene-specific regulatory regions at single-nucleotide resolutionElifeFebruary 25, 2026Alastair J Tulloch, Ryan Nicholas Delgado, Rinaldo Catta-Preta, Constance L Cepkomolar concentration approximately 100-fold higher than fragments in 1x T4 DNA ligase buffer (NEB, cat. #B0202S). The 5’ adapter sequence included an Illumina adapter sequence and a 25-bp homologous sequence for

(18) Massively parallel reporter assay for mapping gene-specific regulatory regions at single-nucleotide resolutioneLifeFebruary 25, 2026Alastair J Tulloch, Ryan Nicholas Delgado, Rinaldo Catta-Preta, Constance L Cepkomolar concentration approximately 100-fold higher than fragments in 1x T4 DNA ligase buffer (NEB, cat. #B0202S). The 5’ adapter sequence included an Illumina adapter sequence and a 25-bp homologous sequence for

(19) ACTG1 facilitates proliferation and epithelial-mesenchymal transition-mediated metastasis via COL21A1 in gastric canceriScienceFebruary 20, 2026Shoumin Li, Bingxue Lan, Li Pan, Ming Sun, Miaomiao Cui, Yun Huang, Sixi Wei, Hai HuangBuffer,Fermentas,Cat#B64 lentiCas9-Blast,Addgene,Cat#52962 lentiGuide-Puro,Addgene,Cat#52963 Quick Ligase Buffer,NEB,Cat#B6058S Quick Ligase,NEB,Cat#M2200S T4 Ligation Buffer,NEB,Cat#B0202S T4 PNK,NEB,Cat#M0201S T4 DNA Ligase,NEB,Cat#M0202 ,, Deposited data,Deposited data,Deposited data ,, RNA-seq

(20) Multichannel genomic recording of biological information with ENGRAMNature ProtocolsFebruary 11, 2026Jenny F. Nathans, Troy A. McDiarmid, Wei Chen, Jay Shendurecat. no. M1100S)T4 DNA ligase (NEB, cat. no. M0202S)10× T4 DNA ligase reaction buffer (NEB, cat. no. B0202S) CRITICAL Multiple freeze-thaw cycles will cause the ATP in the 10x T4 DNA ligase reaction buffer to

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