Advanced DMEM/F-12 from Thermo Fisher Scientific

Product Specs
ItemAdvanced DMEM/F-12
CompanyThermo Fisher Scientific
Price $397.00Get Pricing Get Quote for this Product
Catalog Number12634028
Quantity500 ml
ApplicationsCELL CULTURE
Regulatory StatusFor Research Use Only. Not for use in diagnostic procedures

Specifications/Features

Contains:
Contains 3151 ml/L D-glucose non-essential amino acids (NEAA) and 110 mg/L sodium pyruvate but no L-glutamine.

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Thermo Fisher Scientific

168 Third Avenue

Waltham, MA 02451

United States

Phone: 800-678-5599

Company Profile

Website: www.thermofisher.com

(1) Tumor-targeted bispecific antibodies effectively inhibit oncogenic pathways while minimizing toxicitySci AdvMay 22, 2026Yvonne T. Kschonsak, Wei-Ching Liang, Joyce Chan, David Kan, Thao Nguyen, Karen Ruiz, Yee-Seir Kee, Wen-Ting K. Tsai, Hongkang Xi, Marina Moskalenko, et al.presence of crypts could be confirmed. Afterward, 30 ml of Advanced DMEM/F12 (Thermo Fisher Scientific, 12634028) containing 10% heat-inactivated FBS was added to the dissociation buffer. The crypts were then centrifuged

(2) An autochthonous CRISPR activation screening platform for characterizing tissue-specific oncogene selectionCell Reports MedicineMay 19, 2026Fredrik I. Thege, Amber Hoskins, Annaliese Kramer, Intisar Salim, Ashwath Seetharaman, Natalie Fowlkes, Kimal I. Rajapakshe, Anirban Maitra, Sonja M. Woermannserum,GibcoSigma,Cat# 10082147 Opti-MEM,Thermofisher,Cat# 31985062 Advanced DMEM/F-12,Gibco,Cat# 12634028 HEPES,Gibco,Cat# 15630-080 GlutaMAX,Gibco,Cat# 35-050-061 Sodium pyruvate,Sigma,Cat# S8636 Dimethyl Sulfoxide

(3) Modeling human enterovirus A71 infection using an intestinal microphysiological systemJournal of VirologyMay 19, 2026Hiroki Futatsusako, Sayaka Deguchi, Kaori Kosugi, Rina Hashimoto, Noriyo Nagata, Tadaki Suzuki, Takuya Yamamoto, Kazuo Takayamaand resuspended at 5 × 106 cells/mL in the intestinal differentiation medium (Advanced DMEM/F12 [Cat# 12634028; Thermo Fisher Scientific] supplemented with 2% FBS, 1× GlutaMAX, and 1× penicillin-streptomycin) containing

(4) Glucocorticoids induce a phagocytic C1Q+ macrophage phenotype primed for IFNγ-dependent CXCL9 secretionSci RepMay 18, 2026Alexandra S. Triebig, Tanja Maier, Paul Schwarzlmueller, Sena Atici, Martin E. Kirmaier, Vasileios Chortis, Kleiton S. Borges, David T. Breault, Claudio Ribeiro, Adrian Gottschlich, et al.months. Subsequently, cells were cultured in vitro in Advanced DMEM/F12 medium (Thermo Fisher, Cat# 12634028) supplemented with 2% Nu-Serum (Thermo Fisher CB-5100), 1% ITS (Corning, Cat# 354350), 2 mM L-glutamine

(5) Non-invasive in vivo monitoring of transplanted human intestinal organoids using bioluminescenceSurg Open SciMay 15, 2026Chioma Moneme, Antonio Vinicios Alves da Silva, Prisca C. Obidike, Bryan A. Hogg, Georgia B. Brousseau, Casandra Robinson, Christian Roig-Laboy, José Kleybson de Sousa, Yuwen Zhang, Lily S. Cheng, et al.in the Sato crypt culture medium. The Sato crypt culture medium consisted of Advanced DMEM (Gibco, #12634028), B27 + Insulin (1×) (Gibco, #12587–10), N2 Supplement (1×) (Gibco, #17502–048), 15 mM HEPES Buffer

(6) Modelling kidney cystogenesis using human kidney tubuloid culturesBMC Mol Cell BiolMay 11, 2026Jana Rohe, Arsila Palliyulla Kariat Ashraf, Melina Kehl, Xenia Thielmann, Jörg Ellinger, Glen Kristiansen, Dagmar Wachten, Marieta Ioana Toma(Corning, #431752), resuspended in the collection medium containing Advanced DMEM/F-12 medium (Gibco, #12634028) supplemented with 1x GlutaMAX (Gibco, #35050061), 1 M HEPES (Roth, #9157.1), 100 µg/ml Normocin (InvivoGen,

(7) Keratin 5 marks cancer-propagating cells sustained by an osteopontin-producing niche in high-grade serous ovarian carcinomaCancer Heterogeneity and PlasticityMay 11, 2026Mallikarjun Bidarimath, Coulter Q. Ralston, Nandini Bidarimath, Ian M. Rose, Darianna Colina, Elisa Schmoeckel, Andrew K. Godwin, Doris Mayr, Lora H. Ellenson, Andrea Flesken-Nikitin, et al.washed three times in PBS, placed into 2 mL 2D media (Gibco Advanced DMEM/F12, Thermo Fisher Scientific, 12634028) supplemented with 5% FBS, 12 mM Hepes (Thermo Fisher Scientific, 15630080), 1% L-GlutaMax-I (Thermo Fisher Scientific

(8) Translational 3D in vitro models for immunotherapy testing: from reconstituted organoid co-culture assays to autologous ex vivo patient tissuesFront ImmunolMay 8, 2026Saskia de Man, Ezgi Kaya-Aksoy, Tomas Veenendaal, Niels Meesters, Sergei Chavez Abiega, Xuefei Yan, Michelle Kop, Luca Gandini, Jolie Flach, Jiawei Meng, et al.10 µg/ml IL-2 (200-02, Peptrotech). For EVPT the medium used contains Advanced DMEM F12 (Gibco, cat# 12634028), 2 mM Glutamax (Gibco, cat# 35050038), 100 U/mL PenStrep (Gibco, cat# 15140122), 0.01 M HEPES (Gibco,

(9) Whole-genome doubling drives immune evasion by silencing antigen presentationCancer CellMay 7, 2026Pierre Foidart, Zheqi Li, Xinran Cai, Marco Seehawer, Daniel D. Brown, Amatullah Tawawalla, Pilar Baldominos, Salma Parvin, Jun Nishida, Ernesto Rojas-Jimenez, et al.Anti-Cancer Compound Library,Selleck Chemicals LLC,Cat# L3000 Advanced DMEM/F-12,Life Technologies,Cat# 12634028 Corning™ Minimum Essential Medium Eagle (Mod.) 1X (MEM) with Glutamine,Thermo Fisher Scientific,Cat#

(10) OGDHL promotes prostate cancer progression and regulates neuroendocrine marker expression and nucleotide abundanceMol Cancer ResMay 4, 2026Matthew J. Bernard, Andrea Gallardo, Angel Ruiz, Johnny A. Diaz, Nicholas M. Nunley, Rachel N. Dove, Shile Zhang, Ernie Lee, Kylie Y. Heering, Grigor Varuzhanyan, et al.cells (RRID:CVCL_4853) were cultured in Advanced Dulbecco’s Modified Eagle Medium/Ham’s F-12 (Gibco: 12634028) supplemented with 1% Glutamax (Gibco: 35050061), 10% (v/v) FBS (Sigma-Aldrich: F0926), and 100 units/mL

(11) MDH2 S246 phosphorylation protects mitochondria against hypoxia/reoxygenation injury due to acetate/lactate metabolismThe Korean Journal of Physiology & PharmacologyMay 1, 2026Jubert Marquez, Nammi Park, Maria Victoria Faith Garcia, Jessa Flores, Ippei Shimizu, Hyoung Kyu Kim, Jin HanTakara Bio). Cell culture and transfection AC16 cells were cultured in Dulbecco’s Modified Eagle Medium/F-12 (12634028, Gibco) media with 10% fetal bovine serum (12484028, Gibco) and 100 units/ml penicillin-streptomycin

(12) Myofibroblasts Induce Neuroplasticity to Promote Pancreatic Inflammation and Cancer ProgressionCancer DiscoveryMay 1, 2026Jérémy Nigri, Wenjun Lan, Melanie L. Fung, Charlotte Kayser, Astrid Deschênes, Juliene Hinds, Sanjeev Kaushalya, Sara A. Pawlak, Jennifer S. Thalappillil, Sandeep Nadella, et al.(26) at 37°C for a maximum of 16h. The leftover fibrotic tissues were placed in Advanced DMEM/F12 (12634028, Thermo Fisher Scientific) supplemented with 10% FBS and 1% Penicillin-Streptomycin. hPSCs and hN4 were

(13) Myofibroblasts induce neuroplasticity to promote pancreatic inflammation and cancer progressionCancer DiscovMay 1, 2026Jérémy Nigri, Wenjun Lan, Melanie L. Fung, Charlotte Kayser, Astrid Deschênes, Juliene Hinds, Sanjeev Kaushalya, Sara A. Pawlak, Jennifer S. Thalappillil, Sandeep Nadella, et al.(26) at 37°C for a maximum of 16h. The leftover fibrotic tissues were placed in Advanced DMEM/F12 (12634028, Thermo Fisher Scientific) supplemented with 10% FBS and 1% Penicillin-Streptomycin. hPSCs and hN4 were

(14) Characterization and therapeutic suppression of KEAP1-NRF2-driven resistance to KRAS inhibitors in pancreatic and lung cancerbioRxivApril 24, 2026Wen-Hsuan Chang, Alec J. Vaughan, Addison G. Stamey, Mariana Mancini, Makiko Hayashi, Runying Yang, Ryan Robb, Daniel Andrussier, Jeffrey A. Klomp, Andrew M. Waters, et al.growth factor Matrigel domes with compete human feeding medium (Advanced DMEM/F12 (Thermo Fisher, #12634028)–based WRN conditioned medium (L-WRN)(ATCC, CRL-3276), 100 ng/mL hFGF10 (Thermo Fisher # 100–26), 10

(15) Enforced ZFP281 expression delays breast cancer initiation and can provide lifelong protection against breast cancer metastasisbioRxivApril 18, 2026Deepak K. Singh, Hongwei Zhou, Nyima Sherpa, Xiang Yu Zheng, Artem Lomakin, Pedram Razghandi, Xin Hunag, Rama Kadamb, Suryansh Shukla, Luis E. Valencia Salazar, et al.AF-100–15-A). For organoids, 5 × 105 cells were cultured in organoid medium (advanced DMEM, Gibco, 12634028; 5% FBS, Gemini, 900–108-500; 10 ng/ml EGF; 20 ng/ml Fibroblast Growth Factor 2, R&D Systems, 233FB025;

(16) IFNγ-induced antigen loss in chimeric antigen receptor-T cell therapyFront ImmunolMarch 17, 2026Miao Cao, Jasmine Alvarez, Ramkrishna Mitra, Michael Xu, Trevor R. Baybutt, Zhengyang Sun, Oluwatobiloba Taylor, Allison S. Doermann, Ross E. Staudt, Scott A. Waldman, et al.supplemented with 10% FBS (35-010-CV, Corning). T84 cells were cultured in Advanced DMEM/F-12 50%/50% (12634028, Thermo Fisher Scientific) supplemented with 5% FBS (35-010-CV, Corning). 4.5 Conditioned media production For

(17) IFNγ-induced antigen loss in chimeric antigen receptor-T cell therapyFrontiers in ImmunologyMarch 17, 2026Miao Cao, Jasmine Alvarez, Ramkrishna Mitra, Michael Xu, Trevor R. Baybutt, Zhengyang Sun, Oluwatobiloba Taylor, Allison S. Doermann, Ross E. Staudt, Scott A. Waldman, et al.supplemented with 10% FBS (35-010-CV, Corning). T84 cells were cultured in Advanced DMEM/F-12 50%/50% (12634028, Thermo Fisher Scientific) supplemented with 5% FBS (35-010-CV, Corning). 4.5 Conditioned media production For

(18) Patient-Derived Organoids from Multiple Sites of a Single Tumor Recapitulates Intratumoral Heterogeneity in Patients with Gastric CancerGut and LiverMarch 15, 2026Bo Kyung Yoon, Yoojin Bae, Yeonjin Je, Seyeon Joo, Yuna Kim, Su-Jin Shin, Sungsoon Fang, Jie-Hyun Kimresected cancer tissue was placed in Advanced DMEM/F12 supplemented with 2% antibiotic-antimycotic (Cat#12634028; Gibco, Grand Island, NY, USA) and transported from the operating room to the laboratory in conical

(19) Gpc3 selectively suppresses subcutaneous adipogenesis in diet-induced obesityPLoS BiolMarch 4, 2026Yan Li, Ming Tao, Carlos F. Ibáñez, Meng Xierpm). Digestion was quenched by adding an equal volume of growth medium (Advanced DMEM/F-12 [Gibco, 12634028] supplemented with 10% FBS, 1% GlutaMAX [Thermo, 35050061], 1% MEM NEAA [Thermo, 11140050], and 1% penicillin-streptomycin

(20) Protocol for generating human vascular organoids via orthogonal activation of ETV2 and NKX3.1STAR ProtocolsMarch 1, 2026Liyan Gong, Juan M. Melero-Martin100-0276 FreSR-S,STEMCELL Technologies,Cat# 5859 Advanced DMEM/F-12,Thermo Fisher Scientific,Cat# 12634028 StemPro-34 SFM (1X),Thermo Fisher Scientific,Cat# 10639011 KnockOut DMEM,Thermo Fisher Scientific,Cat#

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Advanced DMEM/F-12 from Thermo Fisher Scientific

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Thermo Fisher Scientific

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Advanced DMEM/F-12 from Thermo Fisher Scientific

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