Knockout DMEM from Thermo Fisher Scientific

Product Specs
ItemKnockout DMEM
CompanyThermo Fisher Scientific
Price $28.75Get Pricing Get Quote for this Product
Catalog Number10829018
Quantity500 mL
Regulatory StatusFor Research Use Only. Not for use in diagnostic procedures

Specifications/Features

Contains:
Knockout™ D-MEM is a basal medium optimized for growth of undifferentiated ES cells (1). The osmolarity is optimized to approximate that of mouse embryonic tissue. Contains no L-glutamine.

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Thermo Fisher Scientific

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Waltham, MA 02451

United States

Phone: 800-678-5599

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Website: www.thermofisher.com

(1) Challenges and solutions in transitioning to animal-free standards: a comprehensive analysis of components in human cell-based developmental neurotoxicity assaysFront ToxicolMay 12, 2026Julia Vanessa Spänle, Lisa Maria Haiber, Bettina Seegerdifferentiated to neural crest cells (NCCs),"Matrigel (#354234, Corning)",EHS-derived,"KnockOut DMEM (#10829018, Gibco)","Contains BSA, insulintransferrin",KnockOut Serum Replacement (KSR) (#108280; Gibco),Contains

(2) Dual role of ZIC2 during neural induction: from priming transcription factor to enhancer activatorNucleic Acids ResApril 25, 2026María Mariner-Faulí, Víctor Sánchez-Gaya, Sarah Malika Robert, Patricia Respuela, Sara de la Cruz-Molina, Sara Lobato-Moreno, Matteo Trovato, Karin D Prummel, Kyung-Min Noh, Judith B Zaugg, et al.gelatin-coated plates using Knockout Dulbecco’s modified Eagle’s medium (DMEM) (Life Technologies, 10829018) supplemented with 15% FBS (Life Technologies, 10082147), leukemia inhibitory factor (LIF), antifungal

(3) Enhanced cell contact and planarian tissue extract increase DNA replication and viability for neoblast cultivationiScienceApril 17, 2026Wenya Zhang, Ling Yang, Nan Li, Kai LeiSiRNeoblasts in different conditions The culture medium,32 comprising KnockOut DMEM (ThermoFisher, 10829018), 100× non-essential amino acid solution (Gibco, 11140050), 100× vitamin solution (Gibco, 11120052),

(4) RAG1-Independent Atypical B cell Development in Chickens: Redefining the Role of RAG1 in Avian ImmunologyiScienceApril 17, 2026Seung Je Woo, Kyung Youn Lee, Jin Lee Lee Kim, Tae Hyun Kim, Jae Yong Hanrecombinant proteins","Chemicals, peptides, and recombinant proteins" ,, Knockout DMEM,Thermo Fisher,Cat#10829018 FBS,Hyclone,Cat#30919.03 Chicken serum,Millipore Sigma,Cat#5405 Nucleoside,Millipore Sigma,Cat#ES-008 Nonessential

(5) Integrated transcriptomic and metabolomic analyses reveal distinct energy metabolic signatures and functional properties of RPE cells under two culture conditionsSci RepApril 10, 2026Fan Zhang, Chenlu Wang, Qinxue Tang, Tangyan Ao, Juan Li, Yong Liu90–100% confluence and then transitioned to KSR medium consisting of 78 ml of knockout DMEM (Gibco 10829018, + 4.5 g/L D-Glucose, -L-Glutamine, + Sodium Pyruvate), 200 µl of 2-mercaptoethanol (Invitrogen), 1

(6) Non-gene-edited neural stem cells reverse neuroinflammation and microbiota dysbiosis in a sprague-dawley rat model of autism spectrum disorderTranslational PsychiatryApril 1, 2026Zhaoming Liu, Caixia Wu, Xianjie Li, Han Wang, Muhammad Majid, Rajesh Basnet, Zhiyuan LiSH30070.03). After 24 h, the medium was replaced with Stage I Induction Medium: KnockOut™ DMEM (Gibco, 10829018) supplemented with 2% B27™ Supplement (Gibco, 17504044), 1% KnockOut™ Serum Replacement (Gibco, 10828028),

(7) Refining fibroblast-to-cardiomyocyte transdifferentiation protocols to explore emergent self-organization in cardiac culturesPLoS OneMarch 23, 2026Elena Turchaninova, Sofya Robustova, Sandaara Kovalenko, Vitalii Dzhabrailov, Aleria Dolgodvorova, Serafima Romanova, Dmitriy Zybin, Mikhail Popov, Alsu Miftakhova, Sheida Frolova, et al.consistent with this protocol. CRM (cardiac reprogramming medium) consisting of KnockOut DMEM (Gibco, 10829018), 15% Fetal Bovine Serum (FBS, Capricorn, FBS-HI-12B), 5% Serum Replacement (SR, Gibco, 10828028), 0.5%

(8) Protocol for generating megakaryocytes from patient induced pluripotent stem cells for disease modeling and compound screeningSTAR ProtocolsMarch 20, 2026Payal Chawla, Niclas Flosdorf, Marcelo A.S. de Toledo, Martin Zenkemedium,Gibco,CAT#12440-053 StemMACS iPS-Brew XF (iPS-Brew),Miltenyi Biotec,CAT#130-104-368 Knockout DMEM,Gibco,CAT#10829018 Knockout serum replacement,Gibco,CAT#10828028 L-Ascorbic acid,Stemcell Technologies,CAT#72132 Matrigel®

(9) KMT2C and KMT2D amplify GRHL2-driven enhancer activationbioRxivMarch 18, 2026Ryan M. Boileau, Kevin X. Chen, Robert BlellochMethods ESC culture and line generation Mouse ESCs were cultured in KnoCKOut DMEM (Thermo Fisher, CAT#10829018) supplemented with 15% FBS, L-Glutamine, Penicillin/Streptomycin, NEAA, LIF (1000U/mL), and 2i (1uM

(10) Poking Pluripotency: Nanoinjection Into Human iPSCsAdvanced MaterialsMarch 14, 2026Jann Harberts, Vuong Thi Thanh Xuan Ho, Yuqi Yang, Yuan Jiang, Roey Elnathan, Nicolas H. Voelckerovernight at RT with Matrigel (Corning, #354263, approx. 19 mg/mL, prediluted at 1:5 in Knockout DMEM, #10829018, ThermoFisher Scientific, final dilution of 1:150) using 1.5 mL per well of a 6‐well plate. Cells were

(11) Generating high-quality porcine iPSCs with the new medium cocktail LACIDStem Cell ReportsMarch 10, 2026Bingbo Shi, Jiajun Li, Xiaomin Wang, Dong Liu, Jinzhu Xiang, Hanning Wang, Changjiang Xu, Xinle Zou, Zhuangfei Wang, Tao Huang, et al.(S)-(+)-dimethindene (1425, Tocris), 50 μg mL−1 vitamin C, which is supplemented with KnockOut DMEM (10829018, Thermo Fisher). piPSCs were passaged every 2 to 3 days using TrypLE Select (12563011, Thermo Fisher)

(12) Post-replicative chromatin accessibility predicts cell fate changeStem Cell ReportsMarch 10, 2026Teresa E. Knudsen, Nazaret Reverón-Gómez, Alva Biran, Nicolas Alcaraz, Sihem Cheloufi, Konrad Hochedlinger, Joshua M. Brickman, Anja Groth, Kathleen R. Stewart-Morganwith 0.2% gelatin (Sigma-Aldrich, G9391) and culturing in reprogramming media: KnockOut DMEM (Gibco, 10829018) supplemented with 15% heat-inactivated FCS (GE Hyclone, SV30160.03), 1× GlutaMAX (Gibco, 35050061),

(13) Human iPSC-derived alveolar macrophages reveal macrophage subtype functions of itaconate in M. tuberculosis defenseJCI InsightMarch 9, 2026Adam S. Krebs, Tomi Lazarov, Anthony T. Reynolds, Kimberly A. Dill-McFarland, Abigail Xie, James M. Bean, Muxue Du, Olivier Levy, John A. Buglino, Aaron Zhong, et al.mouse feeder cells (Thermo Fisher Scientific, A34181) in ESC media (KO DMEM, Thermo Fisher Scientific,10829018) supplemented with 10% KO Serum Replacement (Thermo Fisher Scientific, 10828028), 1% penicillin-streptomycin

(14) Transcriptional derepression of negative regulators of MAP kinase supports maintenance of diapause ES cells in the pluripotent stateGenes & DevelopmentMarch 2, 2026Tuo Zhang, Ryan J. Marina, Rab Prinjha, Karen Adelman, Alexander Tarakhovskyhalf–half ES medium: 40% EmbryoMax DMEM (Millipore SLM-220), 40% KnockOut DMEM (Thermo Fisher Scientific 10829018), 7.5% GemCell fetal bovine serum (Gemini 100-500), 7.5% knockout serum replacement (Thermo Fisher Scientific

(15) Protocol for generating human vascular organoids via orthogonal activation of ETV2 and NKX3.1STAR ProtocolsMarch 1, 2026Liyan Gong, Juan M. Melero-Martin12634028 StemPro-34 SFM (1X),Thermo Fisher Scientific,Cat# 10639011 KnockOut DMEM,Thermo Fisher Scientific,Cat# 10829018 "PuroCol Type I Collagen Solution, 3 mg/ml (Bovine)",Advanced BioMatrix,Cat# 5005 "PBS, pH 7.4",Thermo Fisher Scientific

(16) Protocol for differentiation of vascular smooth muscle cells from human iPSCs and their application in CRISPRa-mediated gene regulationSTAR ProtocolsMarch 1, 2026Laura Priesmeier, Malte Tiburcy, Laura Cecilia Zelarayán521,BioLamina,LN521-05 Retinoic Acid,SigmaAldrich,R2625 Vitronectin,Invitrogen,A14700 KnockOut DMEM,ThermoFisher,10829018 KnockOut Serum,ThermoFisher,10828028 L-Glutamine,ThermoFisher,A2916801 PDGF-beta,ThermoFisher (Peprotech),AF-100-14B TGF-beta

(17) African pygmy mouse iPSCs as a model for in vitro embryogenesis, interspecies chimerism, and blastocyst complementationCell Reports MethodsFebruary 23, 2026Pjeter Gjonlleshaj, Ajda Lenardič, Monika Tarnowska-Sengül, David Taborsky, Christine L. Trautmann, João Agostinho de Sousa, Nicola Bundschuh, Frédéric Veyrunes, Ataman Sendoel, Ori Bar-Nurwere cultured in “fibroblast growth medium” composed of 1:1 Knockout DMEM (Thermo Fisher Scientific, 10829018) and high glucose DMEM (Thermo Fisher Scientific, 41966029), supplemented with 10% Fetal Bovine Serum

(18) PGM1 deficiency is linked to sarcomeric and mitochondrial dysfunction in patient-derived iPSC-cardiomyocytesJournal of Translational MedicineFebruary 21, 2026Silvia Radenkovic, Graeme Preston, Rohit Budhraja, Irena Muffels, Anna Ligezka, Nathan P. Staff, Ron Hrstka, Bijina Balakrishnan, Rameen Shah, Sanne Verberkmoes, et al.(Thermo Fisher Scientific, A1217703), collected in RPMI+insulin supplemented with 10% Knock-Out Serum (KOSR, ThermoFisher 10829018) and 10 µM ROCK inhibitor Y-27,632. The cells were counted and pelleted at 800 rpm, 6 min, room temperature

(19) Non-disruptive in vitro monitoring of cellular states with cell-free DNA methylationGenome BiologyFebruary 17, 2026Anja Hess, Alexander Kovacsovics, Fabian Bachinger, Ludovic Vallier, Helene Kretzmer, Alexander Meissnerfor subsequent experiments. All mESCs were cultured on 0.2% gelatin in knockout DMEM medium (Gibco, 10829018) with 15% FBS, 1% penicillin/streptomycin (ThermoFisher Scientific, 15140122), 1% glutamine (ThermoFisher

(20) Endothelial PDGF Signaling Dysregulation Impairs Testicular Interstitial Homeostasis in DiabetesAdvanced ScienceFebruary 5, 2026Wenxiu Zhang, Kai Hong, Yanling Tang, Lina Cui, Xiaojian Lu, Jianxing Cheng, Yangyi Fang, Qiaoling Jiang, Ziyan Zhuang, Songzhan Gao, et al.of culture medium per well of a 6‐well plate. The culture medium consisted of KO‐DMEM (Invitrogen, 10829018) supplemented with 10% knock‐out serum replacement (Invitrogen, 10828028) and 1% penicillin/streptomycin

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Knockout DMEM from Thermo Fisher Scientific

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Knockout DMEM from Thermo Fisher Scientific

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