Welcome Guest
Sign In
Register
Products
Popular Categories
Antibody Search
ELISA Kit Search
Biomolecules
Flow Cytometers
Thermal Cyclers
Microscopes
Transfection Reagents
Featured Products
New Products
Research Areas
ELISA
Western Blot
3D Cell Culture
Digital PCR
Quantitative PCR
Cell and Gene Therapy
In Vivo Models
Resources
eBooks
Bench Tips
Tech Insights
Editorial Articles
News
Product Reviews
Future Lab
White Papers
Infographics
Events
Promotions
Multimedia
Biocompare Webinars
Technologies Transforming Precision Medicine
Immunotherapy
Reproducibility
Transformative Technologies
Podcasts
Bench Tip Videos
All Videos and Webinars
Life Science Webinars
Write a Review
Antibodies
Kits/Reagents
Equipment
Services
Register
Sign In
Biocompare
Products
Denaturing PAGE Sample Buffers
Blue Loading Buffer Pack
Blue Loading Buffer Pack from Cell Signaling Technology
Be the first to write a review!
Citations:
(105)
$29.00 Inquire
from
Cell Signaling Technology for
Blue Loading Buffer Pack
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Blue Loading Buffer Pack
Company
Cell Signaling Technology
Price
$29.00 Inquire
Catalog Number
7722S
Quantity
1 Pack
Research Area
Related Products
Applications
W
Add to Compare List
Cell Signaling Technology
3 Trask Lane
Danvers
,
Massachusetts
01923
United States
Phone
: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488
Company Profile
Website:
http://www.cellsignal.com/
Website:
Technical Support
Citations (105)
(1)
Tandem RNA and Protein Extraction: A Platform for Maximizing the Use of Limited Ex Vivo Tissue Samples
BIO-PROTOCOL
March 20, 2026
Ciarán Kennedy, Braden Millar, Luke J. Conroy, Mariam Marai, Mary Barry, Martin O’Donohoe, Orina Belton, Eoin Brennan, Catherine Godson, Monica de Gaetano
catalog number: M3148) 2. Laemmli buffer and DTT, 8 mL (
Cell Signaling Technology
, catalog number:
7722S
) 3. Ethanol 200-proof (
Fisher Scientific
, catalog number: CCE7023) 4.
TRK
lysis buffer (EZNA Extraction
(2)
Tandem RNA and Protein Extraction: A Platform for Maximizing the Use of Limited Ex Vivo Tissue Samples
Bio Protoc
March 20, 2026
Ciarán Kennedy, Braden Millar, Luke J. Conroy, Mariam Marai, Mary Barry, Martin O’Donohoe, Orina Belton, Eoin Brennan, Catherine Godson, Monica de Gaetano
catalog number: M3148) 2. Laemmli buffer and DTT, 8 mL (
Cell Signaling Technology
, catalog number:
7722S
) 3. Ethanol 200-proof (
Fisher Scientific
, catalog number: CCE7023) 4.
TRK
lysis buffer (EZNA Extraction
(3)
Mechanisms of HIV Latency in Hematopoietic Progenitors: GFI1 as a Key Regulator
bioRxiv
February 24, 2026
Jackline A. Onyango, Chen Li, Cuie Chen, W Miguel Disbennett, Maria C. Virgilio, Mark M. Painter, Valeri H. Terry, Barkha Ramnani, Joshua D. Welch, Kathleen L. Collins
sequencing.
Western blotting
For western blot
analysis, cells were lysed in Blue Loading Buffer (cat#
7722
,
Cell Signaling Technology
), sonicated with a Misonix sonicator (Qsonica, LLC. Newtown, CT), incubated
(4)
Largely Distinct Post-Translational Modifications Differentiate Skeletal Muscle Wasting Caused by Cancer, Dexamethasone and Aging
J Cachexia Sarcopenia Muscle
February 4, 2026
Anna Stephan, Flavia A. Graca, Suresh Poudel, Yingxue Fu, Yong-Dong Wang, Myriam Labelle, Fabio Demontis
#5000006). Protein samples were prepared for
SDS‐PAGE
by adding SDS‐Blue loading buffer (
Cell Signalling
, #
7722
) and 0.1 M DTT (Cell Signalling, #1425 S) and by heating the samples at 95°C for 5 min. Samples were
(5)
Rab43 mitigates the inflammatory response in acute lung injury via MyD88 ubiquitination
Scientific Reports
January 7, 2026
Yao Wang, Xueping Liu, Zhihao Zhu, Di Wu, Yue Chen, Daohui Gong, Pengfei Wu, Hang Qian, Binfeng He, Guansong Wang
reagents Reagents were as follows: Pierce™ IP Lysis Buffer (#P0013, Beyotime). 3×Blue loading buffer (#
7722
,
CST
). Pierce™ BCA Protein Assay Kit (#
BCA1
,
Sigma-Aldrich
). Protease Inhibitor Cocktail (#04693159001,
(6)
Lipidomic changes in persister cancer cells drive enhanced ferroptosis sensitivity
Ferroptosis and Oxidative Stress
December 29, 2025
Eduard Reznik, Fereshteh Zandkarimi, Joleen M. Csuka, Qiulin Zhu, Jenny Jin, Taruna Vani Neelakantan, Jiewen Zheng, Vasiliki Polychronidou, Mark Fongheiser, Ashley Brown,
et al.
protein concentration and volume. 4x Laemmli buffer containing 125 µM DTT (
Cell Signaling Technology
, #
7722S
) was added to all samples, which were incubated at 100 °C for 10 min. An equal amount of protein in
(7)
Perturbation of multiprotein complexes in skeletal muscle induces protective proteases in the CNS that degrade pathogenic proteins
npj Aging
November 27, 2025
Mamta Rai, Helen J. E. Baddeley, Chia-Lung Chuang, Anna Platt, Michelle Curley, David Finkelstein, Fabio Demontis
samples were heated to 95 °C for four minutes with SDS-Blue loading buffer (
Cell Signaling Technology
, #
7722
) and DTT (Cell Signaling Technology, #1425S), and analyzed by 4–20% SDS-PAGE gels (Mini-PROTEAN TGX
(8)
Proteome solubility is differentially reshaped by thermal stress and regulators of ubiquitination
Journal of Biological Chemistry
July 24, 2025
Liam C. Hunt, Anna Stephan, Suresh Poudel, Kaiwen Yu, Kanisha Kavdia, Vishwajeeth R. Pagala, Wei Wang, Yingxue Fu, Yong-Dong Wang, Xusheng Wang,
et al.
previously (13, 151). Subsequently, the samples were heated with SDS-Blue loading buffer (
Cell Signaling
#
7722
) and DTT (Cell Signaling #1425S), and analyzed by
SDS-PAGE
followed by
Western blotting. The
following
(9)
Energy stress-induced PKCζ S-glutathionylation is essential for LKB1 cytoplasmic translocation and AMPK activation
Life Metabolism
July 16, 2025
Junjie Fei, Shuhan Yu, Mingzhe Xu, Yang Liu, Guoqiang Wang, Xueqing Li, Xinyue Yu, Yifan Zhang, Wenhua Zhang, Yang Wang,
et al.
For western blot
analysis, cells were collected, washed twice with cold PBS, and lysed in 1 × SDS Sample Buffer (#
7722
,
Cell Signaling Technology
, USA) according to the manufacturer’s protocol supplemented with proteasome
(10)
Charting the regulatory landscape of TP53 on transposable elements in cancer
Genome Res
June 1, 2025
Xuan Qu, Yonghao Liang, Colin McCornack, Xiaoyun Xing, Heather Schmidt, Chad Tomlinson, Catrina Fronick, Edward A. Belter, Juan F. Macias-Velasco, Ting Wang
Scientific, A55864). Protein was then denatured with
Blue Loading Buffer Pack
(
Cell Signaling Technology
,
7722S
) and loaded into 10% or 16%
Tris
-Glycine Mini Gels (
Thermo Fisher Scientific
,
XP00105BOX
,
XP00165BOX
).
(11)
LMTK3 regulation of EV biogenesis and cargo sorting promotes tumour growth by reducing monocyte infiltration and driving pro-tumourigenic macrophage polarisation in breast cancer
Mol Cancer
May 23, 2025
Mark Samuels, Christos Karakostas, Simoni Besta, Andrea Lauer Betrán, Katerina Tsilingiri, Charlotte Turner, Reza Shirazi Nia, Niloufar Poudine, Richard Goodyear, William Jones,
et al.
rotation. Elution of the
co-IP
was performed by adding 40 µL of 3 × SDS sample buffer (
Cell Signaling
,
7722
) to the magnetic bead immunocomplex and the samples were incubated at 95 °C for 5 min. The eluates were
(12)
Glioma-neuronal circuit remodeling induces regional immunosuppression
Nat Commun
May 22, 2025
Takahide Nejo, Saritha Krishna, Akane Yamamichi, Senthilnath Lakshmanachetty, Christian Jimenez, Kevin Y. Lee, Donovan L. Baker, Jacob S. Young, Tiffany Chen, Su Su Sabai Phyu,
et al.
lysate samples were denatured by mixing with Blue Loading Gel Dye and DTT (
Cell Signaling Technology
,
7722
). Primary antibodies used in this experiment were as follows:
TSP1
,
anti-Thrombospondin
-1
clone
A6.1
(13)
CREBBP inactivation sensitizes B cell acute lymphoblastic leukemia to ferroptotic cell death upon BCL2 inhibition
Nat Commun
May 20, 2025
Alicia Garcia-Gimenez, Jonathan E. Ditcham, Dhoyazan M. A. Azazi, George Giotopoulos, Ryan Asby, Eshwar Meduri, Jaana Bagri, Nathalie Sakakini, Cecile K. Lopez, Nisha Narayan,
et al.
RIPA buffer lysates were resuspended in boiling loading control with DTT (
Cell Signaling Technology
,
7722
). Cell lysates were separated by
SDS-PAGE
electrophoresis
(NuPAGE 3–8%TRIS acetate protein gels (Invitrogen)
(14)
Human genetics identify convergent signals in mitochondrial LACTB-mediated lipid metabolism in cardiovascular-kidney-metabolic syndrome
Cell Metabolism
January 7, 2025
Shen Li, Hongbo Liu, Hailong Hu, Eunji Ha, Praveena Prasad, Brenita C. Jenkins, Ujjalkumar Subhash Das, Sarmistha Mukherjee, Kyosuke Shishikura, Renming Hu,
et al.
SA00001).
Western blot
Mice kidney or cells lysates were homogenized in SDS lysis buffer (
CST
, no.
7722
) with 2-mercaptoethanol, and boiled for 5 min at 95°C. Lysates were transferred onto PVDF
membranes
.
(15)
SENP3 mediates deSUMOylation of SIX1 to promote prostate cancer proliferation and migration
Cellular & Molecular Biology Letters
December 2, 2024
Zhenlong Shao, Shutong Liu, Wenshuang Sun, Xuefen Zhuang, Shusha Yin, Ji Cheng, Xiaohong Xia, Yuning Liao, Jinbao Liu, Hongbiao Huang
antibody–dynabeads–protein complex was washed three times with PBS-Tween-20 and then mixed with 1× blue loading buffer (#
7722
,
Cell Signaling Technology
). The mixture was heated in a metal
bath
at 70 ℃ for 5 min. The targeted
(16)
Mutational scanning of CRX classifies clinical variants and reveals biochemical properties of the transcriptional effector domain
Genome Research
October 29, 2024
James L. Shepherdson, David M. Granas, Jie Li, Zara Shariff, Stephen P. Plassmeyer, Alex S. Holehouse, Michael A. White, Barak A. Cohen
21,000g for 10 min and the supernatant was mixed with Blue Loading Buffer (
Cell Signaling Technology
7722
) per the manufacturer's recommended ratio. The combined supernatant and loading buffer were boiled for
(17)
Transgenic sensors reveal compartment-specific effects of aggregation-prone proteins on subcellular proteostasis during aging
Cell Reports Methods
October 21, 2024
Michelle Curley, Mamta Rai, Chia-Lung Chuang, Vishwajeeth Pagala, Anna Stephan, Zane Coleman, Maricela Robles-Murguia, Yong-Dong Wang, Junmin Peng, Fabio Demontis
prepared for subsequent analyses by adding SDS-blue loading buffer and DTT (
Cell Signaling Technologies
#
7722
), and by heating them for 5 min at 95°C. The protein concentration of the lysates was determined by
(18)
Multiomics reveals microbial metabolites as key actors in intestinal fibrosis in Crohn’s disease
EMBO Molecular Medicine
September 13, 2024
Xuehua Li, Shixian Hu, Xiaodi Shen, Ruonan Zhang, Caiguang Liu, Lin Xiao, Jinjiang Lin, Li Huang, Weitao He, Xinyue Wang,
et al.
,
Cell Signaling
,
9806S
Protease and phosphatase
inhibitors
,
Cell Signaling
,
5872S
Blue loading Buffer,
Cell Signaling
,
7722S
Software,Software,Software QIIME2 2019.4,https://docs.qiime2.org/2019.4/tutorials/,N/A Cutadapt (version
(19)
Epigenetic therapy potentiates transposable element transcription to create tumor-enriched antigens in glioblastoma cells
Nature Genetics
September 2, 2024
H. Josh Jang, Nakul M. Shah, Ju Heon Maeng, Yonghao Liang, Noah L. Basri, Jiaxin Ge, Xuan Qu, Tatenda Mahlokozera, Shin-Cheng Tzeng, Russell B. Williams,
et al.
washed in ice-cold PBS before being denatured with
Blue Loading Buffer Pack
(
Cell Signaling Technology
,
7722S
). Cell lysates were then loaded into Novex 10%
Tris
-Glycine Mini Gels (
Thermo Fisher Scientific
,
XP00100BOX
),
(20)
HIV-1 Vpr combats the PU.1-driven antiviral response in primary human macrophages
Nature Communications
June 29, 2024
Maria C. Virgilio, Barkha Ramnani, Thomas Chen, W. Miguel Disbennett, Jay Lubow, Joshua D. Welch, Kathleen L. Collins
Watertown, Massachusetts).
Immunoblots
For
western blots
, cells were lysed in Blue Loading Buffer (cat#
7722
,
Cell Signaling Technology
), sonicated with a Misonix sonicator (Qsonica, LLC. Newtown, CT), boiled
Page
<<
<
1
2
3
4
5
>
>>
Reviews
Be the First to Write a Review!
Blue Loading Buffer Pack from Cell Signaling Technology
Price
$29.00
Inquire
Product Specs
Company Info
Citations
Reviews
Back To Top