Blue Loading Buffer Pack from Cell Signaling Technology

Product Specs
ItemBlue Loading Buffer Pack
CompanyCell Signaling Technology
Price $29.00 Inquire
Catalog Number7722S
Quantity1 Pack
Research AreaRelated Products
ApplicationsW

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) Tandem RNA and Protein Extraction: A Platform for Maximizing the Use of Limited Ex Vivo Tissue SamplesBIO-PROTOCOLMarch 20, 2026Ciarán Kennedy, Braden Millar, Luke J. Conroy, Mariam Marai, Mary Barry, Martin O’Donohoe, Orina Belton, Eoin Brennan, Catherine Godson, Monica de Gaetanocatalog number: M3148) 2. Laemmli buffer and DTT, 8 mL (Cell Signaling Technology, catalog number: 7722S) 3. Ethanol 200-proof (Fisher Scientific, catalog number: CCE7023) 4. TRK lysis buffer (EZNA Extraction

(2) Tandem RNA and Protein Extraction: A Platform for Maximizing the Use of Limited Ex Vivo Tissue SamplesBio ProtocMarch 20, 2026Ciarán Kennedy, Braden Millar, Luke J. Conroy, Mariam Marai, Mary Barry, Martin O’Donohoe, Orina Belton, Eoin Brennan, Catherine Godson, Monica de Gaetanocatalog number: M3148) 2. Laemmli buffer and DTT, 8 mL (Cell Signaling Technology, catalog number: 7722S) 3. Ethanol 200-proof (Fisher Scientific, catalog number: CCE7023) 4. TRK lysis buffer (EZNA Extraction

(3) Mechanisms of HIV Latency in Hematopoietic Progenitors: GFI1 as a Key RegulatorbioRxivFebruary 24, 2026Jackline A. Onyango, Chen Li, Cuie Chen, W Miguel Disbennett, Maria C. Virgilio, Mark M. Painter, Valeri H. Terry, Barkha Ramnani, Joshua D. Welch, Kathleen L. Collinssequencing. Western blotting For western blot analysis, cells were lysed in Blue Loading Buffer (cat# 7722, Cell Signaling Technology), sonicated with a Misonix sonicator (Qsonica, LLC. Newtown, CT), incubated

(4) Largely Distinct Post-Translational Modifications Differentiate Skeletal Muscle Wasting Caused by Cancer, Dexamethasone and AgingJ Cachexia Sarcopenia MuscleFebruary 4, 2026Anna Stephan, Flavia A. Graca, Suresh Poudel, Yingxue Fu, Yong-Dong Wang, Myriam Labelle, Fabio Demontis#5000006). Protein samples were prepared for SDS‐PAGE by adding SDS‐Blue loading buffer (Cell Signalling, #7722) and 0.1 M DTT (Cell Signalling, #1425 S) and by heating the samples at 95°C for 5 min. Samples were

(5) Rab43 mitigates the inflammatory response in acute lung injury via MyD88 ubiquitinationScientific ReportsJanuary 7, 2026Yao Wang, Xueping Liu, Zhihao Zhu, Di Wu, Yue Chen, Daohui Gong, Pengfei Wu, Hang Qian, Binfeng He, Guansong Wangreagents Reagents were as follows: Pierce™ IP Lysis Buffer (#P0013, Beyotime). 3×Blue loading buffer (#7722, CST). Pierce™ BCA Protein Assay Kit (#BCA1, Sigma-Aldrich). Protease Inhibitor Cocktail (#04693159001,

(6) Lipidomic changes in persister cancer cells drive enhanced ferroptosis sensitivityFerroptosis and Oxidative StressDecember 29, 2025Eduard Reznik, Fereshteh Zandkarimi, Joleen M. Csuka, Qiulin Zhu, Jenny Jin, Taruna Vani Neelakantan, Jiewen Zheng, Vasiliki Polychronidou, Mark Fongheiser, Ashley Brown, et al.protein concentration and volume. 4x Laemmli buffer containing 125 µM DTT (Cell Signaling Technology, #7722S) was added to all samples, which were incubated at 100 °C for 10 min. An equal amount of protein in

(7) Perturbation of multiprotein complexes in skeletal muscle induces protective proteases in the CNS that degrade pathogenic proteinsnpj AgingNovember 27, 2025Mamta Rai, Helen J. E. Baddeley, Chia-Lung Chuang, Anna Platt, Michelle Curley, David Finkelstein, Fabio Demontissamples were heated to 95 °C for four minutes with SDS-Blue loading buffer (Cell Signaling Technology, #7722) and DTT (Cell Signaling Technology, #1425S), and analyzed by 4–20% SDS-PAGE gels (Mini-PROTEAN TGX

(8) Proteome solubility is differentially reshaped by thermal stress and regulators of ubiquitinationJournal of Biological ChemistryJuly 24, 2025Liam C. Hunt, Anna Stephan, Suresh Poudel, Kaiwen Yu, Kanisha Kavdia, Vishwajeeth R. Pagala, Wei Wang, Yingxue Fu, Yong-Dong Wang, Xusheng Wang, et al.previously (13, 151). Subsequently, the samples were heated with SDS-Blue loading buffer (Cell Signaling #7722) and DTT (Cell Signaling #1425S), and analyzed by SDS-PAGE followed by Western blotting. The following

(9) Energy stress-induced PKCζ S-glutathionylation is essential for LKB1 cytoplasmic translocation and AMPK activationLife MetabolismJuly 16, 2025Junjie Fei, Shuhan Yu, Mingzhe Xu, Yang Liu, Guoqiang Wang, Xueqing Li, Xinyue Yu, Yifan Zhang, Wenhua Zhang, Yang Wang, et al.For western blot analysis, cells were collected, washed twice with cold PBS, and lysed in 1 × SDS Sample Buffer (#7722, Cell Signaling Technology, USA) according to the manufacturer’s protocol supplemented with proteasome

(10) Charting the regulatory landscape of TP53 on transposable elements in cancerGenome ResJune 1, 2025Xuan Qu, Yonghao Liang, Colin McCornack, Xiaoyun Xing, Heather Schmidt, Chad Tomlinson, Catrina Fronick, Edward A. Belter, Juan F. Macias-Velasco, Ting WangScientific, A55864). Protein was then denatured with Blue Loading Buffer Pack (Cell Signaling Technology, 7722S) and loaded into 10% or 16% Tris-Glycine Mini Gels (Thermo Fisher Scientific, XP00105BOX, XP00165BOX).

(11) LMTK3 regulation of EV biogenesis and cargo sorting promotes tumour growth by reducing monocyte infiltration and driving pro-tumourigenic macrophage polarisation in breast cancerMol CancerMay 23, 2025Mark Samuels, Christos Karakostas, Simoni Besta, Andrea Lauer Betrán, Katerina Tsilingiri, Charlotte Turner, Reza Shirazi Nia, Niloufar Poudine, Richard Goodyear, William Jones, et al.rotation. Elution of the co-IP was performed by adding 40 µL of 3 × SDS sample buffer (Cell Signaling, 7722) to the magnetic bead immunocomplex and the samples were incubated at 95 °C for 5 min. The eluates were

(12) Glioma-neuronal circuit remodeling induces regional immunosuppressionNat CommunMay 22, 2025Takahide Nejo, Saritha Krishna, Akane Yamamichi, Senthilnath Lakshmanachetty, Christian Jimenez, Kevin Y. Lee, Donovan L. Baker, Jacob S. Young, Tiffany Chen, Su Su Sabai Phyu, et al.lysate samples were denatured by mixing with Blue Loading Gel Dye and DTT (Cell Signaling Technology, 7722). Primary antibodies used in this experiment were as follows: TSP1, anti-Thrombospondin-1 clone A6.1

(13) CREBBP inactivation sensitizes B cell acute lymphoblastic leukemia to ferroptotic cell death upon BCL2 inhibitionNat CommunMay 20, 2025Alicia Garcia-Gimenez, Jonathan E. Ditcham, Dhoyazan M. A. Azazi, George Giotopoulos, Ryan Asby, Eshwar Meduri, Jaana Bagri, Nathalie Sakakini, Cecile K. Lopez, Nisha Narayan, et al.RIPA buffer lysates were resuspended in boiling loading control with DTT (Cell Signaling Technology, 7722). Cell lysates were separated by SDS-PAGE electrophoresis (NuPAGE 3–8%TRIS acetate protein gels (Invitrogen)

(14) Human genetics identify convergent signals in mitochondrial LACTB-mediated lipid metabolism in cardiovascular-kidney-metabolic syndromeCell MetabolismJanuary 7, 2025Shen Li, Hongbo Liu, Hailong Hu, Eunji Ha, Praveena Prasad, Brenita C. Jenkins, Ujjalkumar Subhash Das, Sarmistha Mukherjee, Kyosuke Shishikura, Renming Hu, et al.SA00001). Western blot Mice kidney or cells lysates were homogenized in SDS lysis buffer (CST, no. 7722) with 2-mercaptoethanol, and boiled for 5 min at 95°C. Lysates were transferred onto PVDF membranes.

(15) SENP3 mediates deSUMOylation of SIX1 to promote prostate cancer proliferation and migrationCellular & Molecular Biology LettersDecember 2, 2024Zhenlong Shao, Shutong Liu, Wenshuang Sun, Xuefen Zhuang, Shusha Yin, Ji Cheng, Xiaohong Xia, Yuning Liao, Jinbao Liu, Hongbiao Huangantibody–dynabeads–protein complex was washed three times with PBS-Tween-20 and then mixed with 1× blue loading buffer (#7722, Cell Signaling Technology). The mixture was heated in a metal bath at 70 ℃ for 5 min. The targeted

(16) Mutational scanning of CRX classifies clinical variants and reveals biochemical properties of the transcriptional effector domainGenome ResearchOctober 29, 2024James L. Shepherdson, David M. Granas, Jie Li, Zara Shariff, Stephen P. Plassmeyer, Alex S. Holehouse, Michael A. White, Barak A. Cohen21,000g for 10 min and the supernatant was mixed with Blue Loading Buffer (Cell Signaling Technology 7722) per the manufacturer's recommended ratio. The combined supernatant and loading buffer were boiled for

(17) Transgenic sensors reveal compartment-specific effects of aggregation-prone proteins on subcellular proteostasis during agingCell Reports MethodsOctober 21, 2024Michelle Curley, Mamta Rai, Chia-Lung Chuang, Vishwajeeth Pagala, Anna Stephan, Zane Coleman, Maricela Robles-Murguia, Yong-Dong Wang, Junmin Peng, Fabio Demontisprepared for subsequent analyses by adding SDS-blue loading buffer and DTT (Cell Signaling Technologies #7722), and by heating them for 5 min at 95°C. The protein concentration of the lysates was determined by

(18) Multiomics reveals microbial metabolites as key actors in intestinal fibrosis in Crohn’s diseaseEMBO Molecular MedicineSeptember 13, 2024Xuehua Li, Shixian Hu, Xiaodi Shen, Ruonan Zhang, Caiguang Liu, Lin Xiao, Jinjiang Lin, Li Huang, Weitao He, Xinyue Wang, et al.,Cell Signaling,9806S Protease and phosphatase inhibitors,Cell Signaling,5872S Blue loading Buffer,Cell Signaling,7722S Software,Software,Software QIIME2 2019.4,https://docs.qiime2.org/2019.4/tutorials/,N/A Cutadapt (version

(19) Epigenetic therapy potentiates transposable element transcription to create tumor-enriched antigens in glioblastoma cellsNature GeneticsSeptember 2, 2024H. Josh Jang, Nakul M. Shah, Ju Heon Maeng, Yonghao Liang, Noah L. Basri, Jiaxin Ge, Xuan Qu, Tatenda Mahlokozera, Shin-Cheng Tzeng, Russell B. Williams, et al.washed in ice-cold PBS before being denatured with Blue Loading Buffer Pack (Cell Signaling Technology, 7722S). Cell lysates were then loaded into Novex 10% Tris-Glycine Mini Gels (Thermo Fisher Scientific, XP00100BOX),

(20) HIV-1 Vpr combats the PU.1-driven antiviral response in primary human macrophagesNature CommunicationsJune 29, 2024Maria C. Virgilio, Barkha Ramnani, Thomas Chen, W. Miguel Disbennett, Jay Lubow, Joshua D. Welch, Kathleen L. CollinsWatertown, Massachusetts). Immunoblots For western blots, cells were lysed in Blue Loading Buffer (cat# 7722, Cell Signaling Technology), sonicated with a Misonix sonicator (Qsonica, LLC. Newtown, CT), boiled

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Blue Loading Buffer Pack from Cell Signaling Technology

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Blue Loading Buffer Pack from Cell Signaling Technology

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