Red Loading Buffer Pack from Cell Signaling Technology

Product Specs
ItemRed Loading Buffer Pack
CompanyCell Signaling Technology
Price $29.00 Inquire
Catalog Number7723S
Quantity1 Pack
Research AreaRelated Products
ApplicationsW

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) Rapid optogenetic manipulation of autophagy reveals that the nuclear pore complex is a robust autophagy substratebioRxivFebruary 3, 2026Payel Mondal, Amanda Cyril, Louis Parham, Dana Mamriev, Igor Wierzbicki, Celina Shen, David J. Gonzalez, Maximiliano A. D'Angelo, Christina G. Towersby adding 1/10 volume 30X DTT (1.25 M DTT, CST#14265) to 1 volume of 3X SDS loading buffer (CST Cat#7723). 50ul of the sample buffer was added to the washed beads and incubated at 96–100°C for 5 minutes. 5–20

(2) Improved Sleep, Memory, and Cellular Pathological Features of Tauopathy, Including the NLRP3 Inflammasome, after Chronic Administration of Trazodone in rTg4510 MiceThe Journal of neuroscience : the official journal of the Society for NeuroscienceApril 20, 2022de Oliveira P, Cella C, Locker N, Ravindran KKG, Mendis A, Wafford K, Gilmour G, Dijk DJ, Winsky-Sommerer R.(Thermo Fisher Scientific). An equivalent amount of protein from each sample was mixed with 3× Red Loading Buffer (catalog #7723, Cell Signaling Technology) with 1.25 m dithiothreitol (Cell Signaling Technology), and incubated at

(3) Metformin reverses chemoresistance in non-small cell lung cancer via accelerating ubiquitination-mediated degradation of Nrf2Translational Lung Cancer ResearchJanuary 25, 2021Sha Huang et al.measure the protein concentration. The lysates were then mixed with 3X reducing SDS loading buffer (#7723, Cell Signaling Technology, Inc., USA) and denatured at 100 °C for 5 min. The lysates were separated

(4) Cranial Suture Regeneration Mitigates Skull and Neurocognitive Defects in CraniosynostosisCellJanuary 7, 2021Yu M, Ma L, Yuan Y, Ye X, Montagne A, He J, Ho TV, Wu Y, Zhao Z, Sta Maria N, et al.other day. Total protein was extracted using a solution of loading buffer (Cell Signaling Technology, 7723S), protease inhibitor (Thermo Fisher Scientific, 1861278) and DTT (Cell Signaling Technology, 7723S),

(5) Affinity-Bead Assisted Mass Spectrometry (Affi-BAMS): A Multiplexed Microarray Platform for Targeted ProteomicsInternational Journal of Molecular SciencesMarch 16, 2020Ghaith M. Hamza, Vladislav B. Bergo, Sergey Mamaev, Don M. Wojchowski, Paul Toran, Camilla R. Worsfold, M. Paola Castaldi, Jeffrey C. Silvalong-term storage. 4.8. Western Blotting Protein Lysates were mixed with SDS-PAGE sample buffer (CST, #7723) and run on 4–20% gradient tris-glycine gels (Life Technologies). Proteins were transferred to nitrocellulose

(6) NF-κB/MAPK activation underlies ACVR1-mediated inflammation in human heterotopic ossificationJCI insightNovember 15, 2018Barruet E, Morales BM, Cain CJ, Ton AN, Wentworth KL, Chan TV, Moody TA, Haks MC, Ottenhoff TH, Hellman J, et al.with LPS (10 ng/ ml). Cells were harvested in Red Loading Buffer (Cell Signaling Technology, catalog 7723) supplemented with 1× protease and phosphatase inhibitor cocktail (Roche Diagnostics, catalogs 4906845001 and

(7) Integrase-Deficient Lentiviral Vector as an All-in-One Platform for Highly Efficient CRISPR/Cas9-Mediated Gene EditingMolecular therapy. Methods & clinical developmentJune 16, 2017Ortinski PI, O'Donovan B, Dong X, Kantor B.by Lowry assay using BSA as a standard. Lysates were mixed with 1× Red Loading Buffer (catalog no. 7723; Cell Signaling Technology), supplemented with 100 mM DTT, and denatured by boiling for 10 min. Subsequently,

(8) Establishment and characterization of an oral tongue squamous cell carcinoma cell line from a never-smoking patientOral oncologyJune 1, 2017Wang SJ, Asthana S, van Zante A, Heaton CM, Phuchareon J, Stein L, Higuchi S, Kishimoto T, Chiu CY, Olshen AB, et al.Cell Signaling Technology, Danvers, MA). Equal amounts of protein were prepared by adding reducing red loading buffer (7723, Cell Signaling Technology) and were resolved by SDS-PAGE (Novex TrisGlycine Gels, EC60055BOX, Thermo Fisher Scientific

(9) Complementary PTM Profiling of Drug Response in Human Gastric Carcinoma by Immunoaffinity and IMAC Methods with Total Proteome AnalysisProteomesAugust 7, 2015Matthew P. Stokes, Charles L. Farnsworth, Hongbo Gu, Xiaoying Jia, Camilla R. Worsfold, Vicky Yang, Jian Min Ren, Kimberly A. Lee, Jeffrey C. Silvanormalized between samples. Samples were mixed with SDS-PAGE sample buffer (Cell Signaling Technology, #7723) and run on 4%–20% gradient tris-glycine gels (Life Technologies). Proteins were transferred to nitrocellulose

(10) Quantitative Profiling of DNA Damage and Apoptotic Pathways in UV Damaged Cells Using PTMScan DirectInternational Journal of Molecular SciencesDecember 21, 2012Matthew P. Stokes, Jeffrey C. Silva, Xiaoying Jia, Kimberly A. Lee, Roberto D. Polakiewicz, Michael J. Comband total protein was normalized between samples. Samples were mixed with SDS-PAGE sample buffer (#7723, Cell Signaling Technology, Danvers, MA, USA) and run on 4%–20% gradient tris-glycine gels (Invitrogen).

(11) Rates and regimes of photochemical ozone production over Central East China in June 2006: a box model analysis using comprehensive measurements of ozone precursorsAtmospheric Chemistry and PhysicsOctober 16, 2009Y. Kanaya, P. Pochanart, Y. Liu, J. Li, H. Tanimoto, S. Kato, J. Suthawaree, S. Inomata, F. Taketani, K. Okuzawa, et al.respectively. The OH concentrations for low-O3 days were higher than those for highAtmos. Chem. Phys., 9, 7711–7723, 2009 O3 days until 11:00 CST, because higher NO concentrations for low-O3 days facilitated conversion

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Red Loading Buffer Pack from Cell Signaling Technology

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Red Loading Buffer Pack from Cell Signaling Technology

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