DAPI from Cell Signaling Technology

Product Specs
ItemDAPI
CompanyCell Signaling Technology
Price $74.00 Inquire
Catalog Number4083S
Quantity1 mg
Research AreaRelated Products
ApplicationsIF-IC, FC-FP

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Integrin-linked kinase promotes hepatic fat accumulation via F-actin stabilization-dependent CD36 plasma membrane localizationiScienceAugust 21, 2026Kakali Ghoshal, Fabian Bock, Nathan C. Winn, Richard L. Printz, Deanna P. Bracy, David H. Wasserman, Roy Zent, Ambra Pozziand Alexa Fluor 488 (Na/K ATPase) secondary antibodies and DAPI (1:1000, Cell Signaling Technology, #4083). The sections or cells were washed twice with PBS, airdried and mounted (ProLong™ Gold Antifade Mountant,

(2) Astrovirus infection dysregulates goblet cell-associated antigen passagesiScienceAugust 21, 2026Jacob Gulman, Nicole Heredia-Osuna, Mané Ohanyan, Alejandro Poot, Cristina Vargas, Victoria Auerbuch, Keely G. McDonald, Rodney D. Newberry, Valerie Cortez,Sigma Aldrich,CAT#D2650 Ketamine Hydrochloride,Sigma,CAT#K4138-10ML DAPI 1mg lyophylized,Cell Signaling,CAT#4083S ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, QIAmp Viral

(3) Matrix stress relaxation drives glioblastoma cell response in viscoelastic biomaterialsSci AdvAugust 21, 2026Sadegh Ghorbani, Michelle S. Huang, Daiyao Zhang, Yueming Liu, Christopher Long, Riley Zwetsloot, Esther A.T. Mozipo, Rameshwar R. Rao, Annika Enejder, Sarah C. Heilshornat 4°C with gentle rocking. 4′,6-Diamidino-2-phenylindole (DAPI; 1 μg/ml, Cell Signaling Technology, 4083s) and phalloidin (Sigma-Aldrich) were included in the secondary antibody solution to stain nuclei and

(4) Disruption of the S1/S2 multibasic cleavage site attenuates infectious bronchitis virus, while S2′ partially restores viral virulence and expands tissue tropismJournal of VirologyAugust 18, 2026Ye Zhao, Jun Zhao, Yingfei Li, Linqing Duan, Rong Liang, Min Huang, Jing Zhao, Guozhong Zhangtemperature in the dark. Nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI; CST, #4083) for 5 min. Following the three final PBS washes, the cells were imaged using a fluorescence microscope. Animal experiment

(5) Combined HS6ST1 and HS6ST2 loss disrupts the formation and migration of gonadotropin-releasing hormone neuronsDevelopmentAugust 15, 2026Roberto Oleari, Federica Amoruso, Antonella Lettieri, Elena Ioannou, Alyssa J. J. Paganoni, Jessica Gimmelli, Sara Campinoti, Stefano Manzini, Christiana Ruhrberg, Anna CariboniJackson Immunoresearch). Nuclei were counterstained with DAPI (1:10, 000; Cell Signaling Technology, 4083). AP-fusion protein-binding assays AP-SEMA3A condition media were prepared as previously described

(6) Phycocyanobilin Attenuates Oligomerized Amyloid β-Induced Neuronal Senescence Through SIRT1-Associated MechanismsNutrientsAugust 6, 2026Mei Chou Lai, Yu-Shun Tzeng, I-Min LiuDanvers, MA, USA) for 1–2 h at room temperature in the dark, followed by DAPI counterstaining (Cat# 4083; Cell Signaling Technology, Danvers, MA, USA). Fluorescence images were captured with an Olympus FV3000

(7) Heterotypic interfacial tension between oncogenic and wild-type populations forms the mechanical basis of tissue-specific oncogenesis in epitheliaElifeAugust 6, 2026Amrapali Datta, Phanindra Dewan, Aswin Anto Puthoor, Tanya Chhabra, Tanishq Tejaswi, Sindhu Muthukrishnan, Akshar Rao, Sumantra Sarkar, Medhavi VishwakarmaµM) "Software, algorithm",SPSS,SPSS,RRID:SCR_002865, Other,DAPI stain,Cell Signaling Technology,Cat# 4083,1:1000 Other,Phalloidin stain,Cell Signaling Technology,Cat# 12877,1:300 "Software, algorithm",ImageJ/Fiji,NIH,RRID:SCR_002285,Image

(8) The essential role of the redox balance in adrenal steroidogenesisArch Endocrinol MetabAugust 1, 2026Juliana Lourenço Gebenlian, Aline Faccioli Bodoni, Fernanda Borchers Coeli-Lacchini, Margaret de de Castro, Sonir Roberto Rauber Antoninisecondary antibody (RRID:AB_2535792) Nuclear staining was accomplished using DAPI (dilution: 1:25000 #4083, Cell Signaling Technology), and the slides were set with ProLong™ Diamond (Thermo Fisher Scientific).

(9) Endothelial YAP Signaling Promotes Blood-Spinal Cord Barrier Repair in Mice After Spinal Cord InjuryAdv Sci (Weinh)July 31, 2026Jiawei Wang, Yaozhi He, Yanjiao Wang, Jingjing Zhang, Qishun Liang, Mengxian Jia, Yumin Wu, Zongjie Yuan, Ziwei Fan, Yuxuan Li, et al.species‐appropriate fluorescent secondary antibodies together with the nuclear dye DAPI (1:1, 000, #4083, CST) for 1 h at room temperature. Fluorescent images were acquired using either a fluorescence microscope

(10) Quiescent tumor cells shape the immunosuppressive microenvironment in pancreatic cancerNature CommunicationsJuly 21, 2026B. McClellan, Q. Wang, K. Aung, W. Matsuior (1 μg ml−1 of 4’, 6-diamidino-2-phenylindole, dihydrochloride (DAPI) (Cell signaling Technology, 4083)), washed, and then re-suspended in PBS with 1% FBS prior to FACS. For identification of mVenusHigh

(11) Oxyresveratrol Suppresses EGF-Induced AKT Phosphorylation and Reduces Cellular Fitness While Promoting Apoptosis in EGFR-Wild-Type Non-Small Cell Lung Cancer Cells Under EGF StimulationInt J Mol SciJuly 18, 2026Wutigri Nimlamool, Jatuporn Polhiran, Nitchakarn Phimthong, Saranyapin Potikanond, Nitwara Wikan1:400), DAPI (4′,6–diamidino–2–phenylindole; Cell Signaling Technology) for nuclear staining (Cat# 4083; 1 µg/mL), and DyLight–594–Phalloidin (Cell Signaling Technology) for detection of filamentous actin

(12) Naive CD4+ T cells synthesize a hyaluronan-rich glycocalyx that is diminished following activationiScienceJuly 17, 2026Laxmi Prasad Adhikari, Jessica Contreras, Kristen M. Patterson, Meghan M. Moran, Erin N. Howe, Anna H.K. Plaas, Joseph M. ReynoldsStreptavidin Alexa Fluor 647 conjugate (Invitrogen Cat# S21374) for 1 h, and finally DAPI (1:1000; Cell Signaling Technology Cat# 4083S) for 10 min, on ice and protected from light. For VCAN and HA immunostaining, cells were first incubated

(13) Endothelial epigenetic senescence driven microglial activation mediates cardio-retinal neuroinflammation in heart failureTheranosticsJuly 13, 2026Mengdan Wang, Shuo Zhang, Tursunjan Aziz, Shiyao Zhang, Xiao Wu, Gang Li, Yan Wangin cold acetone (-20 °C, 10 min), incubated with 5 μg/mL WGA (Sigma, L4895) and DAPI (1 μg/mL; CST, 4083) for 30 min, and imaged on a Leica SP8 DLS confocal microscope. RNA extraction and qPCR analysis Total

(14) From Gut to Fat: Intestinal Epithelial Exosomes Target PDGFRα + Progenitors to Promote Lipogenesis and Counteract Subcutaneous Adipose Tissue Atrophy in AgingAging CellJuly 12, 2026Tingting Huang, Ye Huang, Yin Zhou, Xuanbei Lu, Lijun Yang, Jing Yu, Yunlu Sheng, Fan Xia, Guoxian Ding, Yifan Lv, et al.incubated with secondary antibodies, washed again, counterstained with DAPI (Cell Signaling Technology, Cat#4083S), and mounted. 4.14 Bodipy Staining Differentiated FAPs were washed with PBS, fixed in 4% paraformaldehyde

(15) MGF100 but not MGF300 family is a potential multigene-deleted target for ASFV attenuation and live attenuated vaccine developmentVirulenceJuly 6, 2026Wen Dang, Fan Xu, Yu Du, Tao Li, Huanan Liu, Zhengwang Shi, Hong Tian, Jijun He, Haixue ZhengAnti-rabbit IgG (H+L), F(ab’) 2 Fragment (Alexa Fluor® 594 Conjugate) (catalog no. 8889S) and DAPI (catalog no. 4083S) were purchased from CST. Anti-β-Actin mouse monoclonal antibody (1C7) was purchased from Abbkine (catalog

(16) A peripheral blood-based approach involving vimentin along with AURKA enabled efficient tracking of elusive Oct4/Sox2-expressing disseminated breast cancer stem cellsBiosci RepJune 24, 2026Debomita Sengupta, Debanjan Thakur, Elizabeth Mahapatra, Salini Das, Jayanta Chakrabarti, Sagar Sen, Sutapa Mukherjeedilution and thereafter washed in PBS. Finally, the cells were stained in DAPI (Cell Signaling Technology, 4083S) and assessed under a fluorescence microscope (Leica) at 20× magnification or an Olympus laser scanning

(17) Molecular Mechanisms and Network Pharmacology Revealing Therapeutic Potential of Acetamidosulfonamides against Parkinsonian ModelACS OmegaJune 2, 2026Waralee Ruankham, Veda Prachayasittikul, Ratchanok Pingaew, Tanawut Tantimongcolwat, Apilak Worachartcheewan, Kanyarat Chompon, Somsak Ruchirawat, Virapong Prachayasittikul, Supaluk Prachayasittikul, Kamonrat PhopinInc., MA, USA) incubation for 2 h at room temperature. To specify the nucleus localization, DAPI (#4083, Cell Signaling Technology Inc., MA, USA) was used for an additional 10 min of incubation at room temperature.

(18) Lactate regulates osteoclastogenesis via H3k18la in osteoarthritisInt J Mol MedJune 2, 2026Zhibo Fan, Qiyue Fu, Tao Sun, Yutong Zhong, Yuefan Ma, Jingwei Jian, Shuosheng Yuan, Shenghong Li, Xiaomei XuInc.; cat. no. FP1480). Nuclei were counterstained with DAPI (Cell Signaling Technology, Inc.; cat. no. 4083) for 10 min at room temperature and slides were mounted with ProLong™ Gold Antifade reagent (Thermo Fisher Scientific

(19) The C9orf72/SMCR8 complex maintains microglial homeostasis via RAB8A-ESCRT-mediated lysosomal repairThe EMBO JournalMay 29, 2026Shan Li, Shidong Xu, Feng Li, Qirui Zhao, Penghui Zhang, Qinghua Guan, Xiangxiang Sun, Jundong Bi, Hu Xiao, Yiyuli Tang, et al.DNase I,Sigma,DN25 GM-CSF,MCE,HY-P7361 Poly-D-lysine,Sigma,P2636 "4,6-diamidino-2-phenylindole (DAPI)",CST,4083S Anti-fade mounting medium,Beyotime,P0126 Anti-HA magnetic beads,Thermo Fisher Scientific,88836 Glutathione

(20) KRASG12V/A146T mutations are associated with nCRT resistance via enhanced DNA double-strand break repair and support a deep learning prediction framework in LARCCellular & Molecular Biology LettersMay 28, 2026Hengchang Liu, Dechao Bu, Guanhua Yu, Ran Wei, Hui Jin, Yixiao Liu, Xu Guan, Zhixun Zhao, Haipeng Chen, Yi Zhao, et al.antibody (ab150077, Abcam, UK) at room temperature for 1 h, followed by nuclear staining with DAPI (4083S, CST, USA) for 20 min. Images were acquired using a fluorescence microscope. Comet assay Comet assays

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DAPI

DAPI from Cell Signaling Technology

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