DAPI from Cell Signaling Technology

Product Specs
ItemDAPI
CompanyCell Signaling Technology
Price $74.00 Inquire
Catalog Number4083S
Quantity1 mg
Research AreaRelated Products
ApplicationsIF-IC, FC-FP

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

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Website: http://www.cellsignal.com/

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(1) OptoTAT reveals microtubule acetylation as a rapid trigger for GEF-H1-mediated cell migrationJ Cell BiolMay 4, 2026Abhijit Deb Deb Roy, Cristian Saez Gonzalez, Milda Stanislauskas, Farid Shahid, Eesha Yadav, Jalil Rezek, Takanari InoueA11057, A21058, A21096, A11032), Phalloidin (ThermoFisher A22286) and DAPI (Cell Signaling Technology, 4083S). After that, they were washed three times in PBS and the images were captured by microscopy. For HeLa cells

(2) Targeting adipocyte ESRRA alleviates osteoarthritis via interrupting inter-organelle crosstalk of complement C3-CFD-MAC cascadeBone ResApril 29, 2026Tongling Huang, Zihui Wang, Lu Gao, Jun Gao, Zhaocheng Lu, Pengda Li, Chon Him Choy, Zhuolei Yuan, Yanting Zhong, Chang-An Geng, et al.Goat Anti-Rabbit IgG H&L (Alexa Fluor 555) (Abcam #ab150078). Nuclei were counterstained with DAPI (Cell Signaling Technology #4083). After two washes with PBS, sections were dried and mounted with an anti-fade reagent (Invitrogen #P10144).

(3) IDH2 lactylation regulates mitochondrial dysfunction injury induced by myocardial ischemia‑reperfusion via the AMPK signaling pathwayInt J Mol MedApril 17, 2026Changsen Wang, Siman Shen, Suyun Chen, Jinda Lin, Simeng Li, Jianning Chen, Shuyun Cai, Xiaodong Yuan, Ziqiang Yang, Kun Ding, et al.dilution of 1:500 for 1 h at room temperature in the dark. Nuclei were counterstained with DAPI (cat. no. 4083S; Cell Signaling Technology, Inc.; diluted to 5×10−4 mg/ml) for 5 min. Slides were mounted with antifade

(4) Mono- and co-infections of primary porcine respiratory cells with Bordetella bronchiseptica and Streptococcus suis are not affected by the dermonecrotic toxinInfection and ImmunityApril 15, 2026Désirée Schaaf, Muriel Dresen, Yenehiwot Berhanu Weldearegay, Jeannine Biermann, Susan L. Brockmeier, Wolfgang Baumgärtner, Michael Jarek, Peter Valentin-Weigandwere stained with 4′,6-diamidino-2-phenylindole (DAPI, 0.5 μg/mL; Cell Signaling Technology, Cat. No. 4083). Finally, the membrane was cut out and mounted with ProLong Gold Antifade Reagent (Cell Signaling Technology,

(5) Accumulation of mutations in nsp4, E, and the S2 subunit underlies mammalian cell tropism expansion and virulence attenuation of avian coronavirusPLoS PathogApril 9, 2026Yingfei Li, Xuehui Zhang, Rong Liang, Ruotong Li, Ruihua Yang, Liwei Zhang, Ye Zhao, Guozhong Zhang, Jing Zhaotemperature. Nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI; Cell Signaling Technology, #4083) for 10 min at room temperature. Fluorescence images were acquired using a Nikon microscope. Western blot

(6) GV1001 Reprograms CD47 Immune Checkpoint to Restore Macrophage Antitumor Activity in Oral Squamous Cell CarcinomaInt J Mol SciApril 7, 2026Wei Chen, Seojin Kim, Cheyenne Beheshtian, Angela Jun, Sangjae Kim, No-Hee Parkcat. no. A11007) for 1 h. Nuclei were counterstained with DAPI (Cell Signaling Technology, cat. no. 4083, Danvers, MA, USA). Fluorescent images were acquired using a confocal laser-scanning microscope (LSM

(7) Ursodeoxycholic Acid Attenuates Lipopolysaccharide-Induced Myocardial Injury by Inhibiting Oxidative Stress, Inflammation, and Apoptosis: The Interplay of Sirt1/Nrf2 and Akt/NF-κB Signaling PathwaysInt J Mol SciMarch 20, 2026Ranko Škrbić, Tatjana Milivojac, Milkica Grabež, Ljiljana Amidžić, Zorislava Bajic, Tanja Sobot, Nebojša Mandić-Kovačević, Snežana Uletilović, Đorđe Đukanović, Milica Gajic Bojic, et al.temperature. Nuclei were stained with 4′,6-diamidino-2-phenylindole ((DAPI), Cell Signaling Technology, 4083, Danvers, MA, USA) and imaged using a fluorescent microscope (Leica DM 6000B, Wetzlar, Germany). Fluorescence

(8) IRX4204 sensitizes multiple myeloma to ferroptosis and improves lenalidomide efficacy through the HMOX1-GPX4 axisScientific ReportsMarch 17, 2026Jian Wu, Zhibo Yan, Kimberly Burcher, Zhannan Han, Mikhail A. Nikiforov, Vidyasagar Vuligonda, Martin Sanders, Yubin Kanganti-rabbit secondary antibody (Thermo Fisher, R37117). Nuclei were stained with DAPI (Cell Signaling 4083) and coverslipped with antifade medium (Vector Laboratories). Images were obtained using a Leica SP5

(9) KAT2B-mediated epigenetic suppression of RAD51C enhances olaparib sensitivity in colorectal cancerCancer Chemother PharmacolMarch 14, 2026Xiaoqin Liu, Jun Chen, Xuwei Shen, Lin Liwas followed by a 20-minute staining process in which DAPI (from Cell Signalling, catalogue number 4083 S) was used to visualize the nuclei. Finally, the coverslips were washed and mounted with anti-fade

(10) Investigating the Effect and Mechanism of Protocatechuic Aldehyde on Vascular Dementia Based on Multi-Omics ApproachBiomoleculesMarch 11, 2026Tian Xiao, Jie Tao, Jiaoyang Tan, Xingjie Yi, Ziyi Wang, Xiaohua DuanPA, USA), MCT4, GFAP, NeuN (22787-1-AP, 60190-1-Ig, 26975-1-AP, Proteintech, Chicago, IL, USA), DAPI (4083, Cell Signaling Technology, Danvers, MA, USA), dilution ratio 1:200, and incubated at 4 °C overnight.

(11) MSTN and TCF12 as Candidate Immunometabolic Signatures in Glioma-Associated Foam Cells: Insights from Integrated Multi-Omics AnalysisCurr Issues Mol BiolMarch 9, 2026Xu Liu, Zhuo Song, Zhijia Sun, Chen Liu, Xiaoli Kang, Huilian Qiao, Xinzhuo Tu, Teng Li, Zhiguang Fu, Yingjie Wang5220-0336 and 5220-0341, MA, USA). Nuclei were counterstained with DAPI (1:4000; Cell Signaling Technology, 4083, MA, USA). Images were acquired using a Zeiss Axio Scope A1 Fluorescence Microscope (Carl Zeiss AG,

(12) Early life adversity impairs visually evoked innate defensive behaviors via oxytocin signalingCommunications BiologyFebruary 21, 2026Huiying Tan, Junying Su, Shaohua Ma, Shanping Chen, Qingqing Liu, Xing Yang, Liping Wangfor Oxtr staining. Finally, the sections were incubated in 1‰ DEPC-treated PBS with DAPI (1:5000, Cat#4083S, Cell Signaling Technology, USA). Imaging was performed using a virtual microscopy slide-scanning system

(13) Transcriptomic and metabolomic insights into gabapentin’s therapeutic role in neurogenic inflammation of rosaceaCommunications BiologyFebruary 14, 2026Ziqi Jiang, Tian Ding, Yan Zhao, Mao Luo, Yishu Tang, Yuxuan Zhang, Bin Weiwith PBS again. Finally, the samples were mounted with an anti-fade mounting medium containing DAPI (4083, CST) or Hoechst (4082, CST), and images were acquired and observed under a laser scanning confocal

(14) Human lung organoids model for assessing host response to Mycobacterium tuberculosis infectionFrontiers in Cellular and Infection MicrobiologyFebruary 10, 2026Chaofan Li, Pengfei Zhong, Zhimin Yun, Leiming Fang, Meida Xiang, Qisheng Su, Jiru Wang, Hebing Chen, Zhi Chen, Liang Yue, et al.(Thermo Scientific) for 1 hour in the dark, and counterstained with DAPI (Cell Signaling Technology, 4083S) for 15 minutes. Finally, the sections were mounted with an anti-fade mounting medium (Dako, S3023)

(15) Kaempferol Alleviates Glucocorticoid-Induced Osteonecrosis of the Femoral Head by Modulating Macrophage M1/M2 Polarization Through RhoA/ROCK-Mediated Mitophagy ActivationBiomedicinesFebruary 9, 2026Yuankai Zhang, Yan Zhao, Shangqing Zhang, Tian Lei, Bocheng Xiang, Xin Zhang, Kai Nan, Lihong Fanwere introduced for 2 h at ambient temperature. Subsequently, tissue sections received DAPI staining (4083S, CST). Protein expression levels were then assessed via laser confocal microscopy. 2.10. Immunohistochemical Staining

(16) Triple targeting of STING, TGF-β, and PD-L1 boosts CXCL16-CXCR6 signaling for potent antitumor responseNat CommunFebruary 9, 2026Ming Yi, Tianye Li, Yinhui Gu, Mengke Niu, Dixuan Xue, Shengtao Hu, Yuze Wu, Bin Zhao, Di Zhang, Yingkang Ma, et al.used for multiplex IF are listed in Table S2. After staining, sections were counterstained with DAPI (4083, CST), mounted with an antifade reagent, and imaged using a confocal microscope. Image analysis was

(17) Integrin-Mediated Mechanosensing of Modeled Lymph Node Microenvironment Promotes T Cell Activation via Nuclear DeformationResearch (Wash D C)February 6, 2026jinteng feng, Guoqing Zhao, Lingzhu Zhao, Luying Geng, Shirong Zhang, Longwen Xu, Mengjie Liu, Guangjian Zhang, Feng Xu, Min Lin, et al.rhodamine phalloidin (1:500; R415, Invitrogen, USA). 4′,6-Diamidino-2-phenylindole (DAPI; 0.5 μg/ml; 4083, Cell Signaling Technology, USA) was used to stain cell nuclei. For enzyme-linked immunosorbent assay

(18) Formyl peptide receptor 2 activation by MR-39 inhibits glioblastoma cell proliferation and invasiveness through suppression of multiple oncogenic pathwaysJournal of Translational MedicineFebruary 6, 2026Maria Grazia Ferraro, Marco Bocchetti, Marianna Affinito, Ercolano Giuseppe, Kardelen Dalim Filiz, Luisa Speranza, Enza Lacivita, Marcello Leopoldo, Marianna Crispino, Anna Ceccarelli, et al.Cell Signaling #4412). In both cases, nuclei were counterstained with DAPI (1:10000, Cell Signaling #4083), and fluorescence images were acquired using a confocal microscope (Zeiss LSM 900 Airyscan 2) with

(19) Nanoplastics Impair GnRH Neuron Migration and Neuroendocrine Function: Emerging Players in the Pathogenesis of Reproductive DisordersSmallFebruary 6, 2026Federica Amoruso, Alyssa Julia Jennifer Paganoni, Astrid Saraceni, Andrea Magnani, Alessia Brossa, Giorgio Roberto Merlo, Cristina Matei, Ruben Heinjan Willemsen, Raíssa Carneiro Rezende, Alexander Augusto de Lima Jorge, et al.counterstained DAPI (4′,6‐diamidin‐2‐phenylindole, 1:10,000; Cell Signaling, Leiden, Netherlands; cat. 4083). To detect F‐actin filaments, cells were stained with TRITC‐conjugated phalloidin (1:400; Merck, Darmstadt,

(20) Kindlins regulate integrin- and growth factor-dependent ureteric bud formationDevelopmentFebruary 1, 2026Shensen Li, Fabian Bock, Olga Viquez, Anjana Hassan, Sijo Mathew, Riya Palamuttam, Glenda Mernaugh, Xinyu Dong, Meiling Melzer, Matthew Tantengco, et al.Alexa Fluor 555 anti-rat (ThermoFisher, 78945; 1:200) secondary antibodies and DAPI (Cell Signaling, 4083) were used subsequently. Immunofluorescence images were taken on a Zeiss LSM 980 confocal microscope

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