DAPI from Cell Signaling Technology

Product Specs
ItemDAPI
CompanyCell Signaling Technology
Price $74.00 Inquire
Catalog Number4083S
Quantity1 mg
Research AreaRelated Products
ApplicationsIF-IC, FC-FP

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Integrin-linked kinase promotes hepatic fat accumulation via F-actin stabilization-dependent CD36 plasma membrane localizationiScienceAugust 21, 2026Kakali Ghoshal, Fabian Bock, Nathan C. Winn, Richard L. Printz, Deanna P. Bracy, David H. Wasserman, Roy Zent, Ambra Pozziand Alexa Fluor 488 (Na/K ATPase) secondary antibodies and DAPI (1:1000, Cell Signaling Technology, #4083). The sections or cells were washed twice with PBS, airdried and mounted (ProLong™ Gold Antifade Mountant,

(2) Heterotypic interfacial tension between oncogenic and wild-type populations forms the mechanical basis of tissue-specific oncogenesis in epitheliaElifeAugust 6, 2026Amrapali Datta, Phanindra Dewan, Aswin Anto Puthoor, Tanya Chhabra, Tanishq Tejaswi, Sindhu Muthukrishnan, Akshar Rao, Sumantra Sarkar, Medhavi VishwakarmaµM) "Software, algorithm",SPSS,SPSS,RRID:SCR_002865, Other,DAPI stain,Cell Signaling Technology,Cat# 4083,1:1000 Other,Phalloidin stain,Cell Signaling Technology,Cat# 12877,1:300 "Software, algorithm",ImageJ/Fiji,NIH,RRID:SCR_002285,Image

(3) The essential role of the redox balance in adrenal steroidogenesisArch Endocrinol MetabAugust 1, 2026Juliana Lourenço Gebenlian, Aline Faccioli Bodoni, Fernanda Borchers Coeli-Lacchini, Margaret de de Castro, Sonir Roberto Rauber Antoninisecondary antibody (RRID:AB_2535792) Nuclear staining was accomplished using DAPI (dilution: 1:25000 #4083, Cell Signaling Technology), and the slides were set with ProLong™ Diamond (Thermo Fisher Scientific).

(4) Endothelial YAP Signaling Promotes Blood-Spinal Cord Barrier Repair in Mice After Spinal Cord InjuryAdv Sci (Weinh)July 31, 2026Jiawei Wang, Yaozhi He, Yanjiao Wang, Jingjing Zhang, Qishun Liang, Mengxian Jia, Yumin Wu, Zongjie Yuan, Ziwei Fan, Yuxuan Li, et al.species‐appropriate fluorescent secondary antibodies together with the nuclear dye DAPI (1:1, 000, #4083, CST) for 1 h at room temperature. Fluorescent images were acquired using either a fluorescence microscope

(5) Oxyresveratrol Suppresses EGF-Induced AKT Phosphorylation and Reduces Cellular Fitness While Promoting Apoptosis in EGFR-Wild-Type Non-Small Cell Lung Cancer Cells Under EGF StimulationInt J Mol SciJuly 18, 2026Wutigri Nimlamool, Jatuporn Polhiran, Nitchakarn Phimthong, Saranyapin Potikanond, Nitwara Wikan1:400), DAPI (4′,6–diamidino–2–phenylindole; Cell Signaling Technology) for nuclear staining (Cat# 4083; 1 µg/mL), and DyLight–594–Phalloidin (Cell Signaling Technology) for detection of filamentous actin

(6) Naive CD4+ T cells synthesize a hyaluronan-rich glycocalyx that is diminished following activationiScienceJuly 17, 2026Laxmi Prasad Adhikari, Jessica Contreras, Kristen M. Patterson, Meghan M. Moran, Erin N. Howe, Anna H.K. Plaas, Joseph M. ReynoldsStreptavidin Alexa Fluor 647 conjugate (Invitrogen Cat# S21374) for 1 h, and finally DAPI (1:1000; Cell Signaling Technology Cat# 4083S) for 10 min, on ice and protected from light. For VCAN and HA immunostaining, cells were first incubated

(7) Endothelial epigenetic senescence driven microglial activation mediates cardio-retinal neuroinflammation in heart failureTheranosticsJuly 13, 2026Mengdan Wang, Shuo Zhang, Tursunjan Aziz, Shiyao Zhang, Xiao Wu, Gang Li, Yan Wangin cold acetone (-20 °C, 10 min), incubated with 5 μg/mL WGA (Sigma, L4895) and DAPI (1 μg/mL; CST, 4083) for 30 min, and imaged on a Leica SP8 DLS confocal microscope. RNA extraction and qPCR analysis Total

(8) From Gut to Fat: Intestinal Epithelial Exosomes Target PDGFRα + Progenitors to Promote Lipogenesis and Counteract Subcutaneous Adipose Tissue Atrophy in AgingAging CellJuly 12, 2026Tingting Huang, Ye Huang, Yin Zhou, Xuanbei Lu, Lijun Yang, Jing Yu, Yunlu Sheng, Fan Xia, Guoxian Ding, Yifan Lv, et al.incubated with secondary antibodies, washed again, counterstained with DAPI (Cell Signaling Technology, Cat#4083S), and mounted. 4.14 Bodipy Staining Differentiated FAPs were washed with PBS, fixed in 4% paraformaldehyde

(9) MGF100 but not MGF300 family is a potential multigene-deleted target for ASFV attenuation and live attenuated vaccine developmentVirulenceJuly 6, 2026Wen Dang, Fan Xu, Yu Du, Tao Li, Huanan Liu, Zhengwang Shi, Hong Tian, Jijun He, Haixue ZhengAnti-rabbit IgG (H+L), F(ab’) 2 Fragment (Alexa Fluor® 594 Conjugate) (catalog no. 8889S) and DAPI (catalog no. 4083S) were purchased from CST. Anti-β-Actin mouse monoclonal antibody (1C7) was purchased from Abbkine (catalog

(10) A peripheral blood-based approach involving vimentin along with AURKA enabled efficient tracking of elusive Oct4/Sox2-expressing disseminated breast cancer stem cellsBiosci RepJune 24, 2026Debomita Sengupta, Debanjan Thakur, Elizabeth Mahapatra, Salini Das, Jayanta Chakrabarti, Sagar Sen, Sutapa Mukherjeedilution and thereafter washed in PBS. Finally, the cells were stained in DAPI (Cell Signaling Technology, 4083S) and assessed under a fluorescence microscope (Leica) at 20× magnification or an Olympus laser scanning

(11) Molecular Mechanisms and Network Pharmacology Revealing Therapeutic Potential of Acetamidosulfonamides against Parkinsonian ModelACS OmegaJune 2, 2026Waralee Ruankham, Veda Prachayasittikul, Ratchanok Pingaew, Tanawut Tantimongcolwat, Apilak Worachartcheewan, Kanyarat Chompon, Somsak Ruchirawat, Virapong Prachayasittikul, Supaluk Prachayasittikul, Kamonrat PhopinInc., MA, USA) incubation for 2 h at room temperature. To specify the nucleus localization, DAPI (#4083, Cell Signaling Technology Inc., MA, USA) was used for an additional 10 min of incubation at room temperature.

(12) Lactate regulates osteoclastogenesis via H3k18la in osteoarthritisInt J Mol MedJune 2, 2026Zhibo Fan, Qiyue Fu, Tao Sun, Yutong Zhong, Yuefan Ma, Jingwei Jian, Shuosheng Yuan, Shenghong Li, Xiaomei XuInc.; cat. no. FP1480). Nuclei were counterstained with DAPI (Cell Signaling Technology, Inc.; cat. no. 4083) for 10 min at room temperature and slides were mounted with ProLong™ Gold Antifade reagent (Thermo Fisher Scientific

(13) The C9orf72/SMCR8 complex maintains microglial homeostasis via RAB8A-ESCRT-mediated lysosomal repairThe EMBO JournalMay 29, 2026Shan Li, Shidong Xu, Feng Li, Qirui Zhao, Penghui Zhang, Qinghua Guan, Xiangxiang Sun, Jundong Bi, Hu Xiao, Yiyuli Tang, et al.DNase I,Sigma,DN25 GM-CSF,MCE,HY-P7361 Poly-D-lysine,Sigma,P2636 "4,6-diamidino-2-phenylindole (DAPI)",CST,4083S Anti-fade mounting medium,Beyotime,P0126 Anti-HA magnetic beads,Thermo Fisher Scientific,88836 Glutathione

(14) KRASG12V/A146T mutations are associated with nCRT resistance via enhanced DNA double-strand break repair and support a deep learning prediction framework in LARCCellular & Molecular Biology LettersMay 28, 2026Hengchang Liu, Dechao Bu, Guanhua Yu, Ran Wei, Hui Jin, Yixiao Liu, Xu Guan, Zhixun Zhao, Haipeng Chen, Yi Zhao, et al.antibody (ab150077, Abcam, UK) at room temperature for 1 h, followed by nuclear staining with DAPI (4083S, CST, USA) for 20 min. Images were acquired using a fluorescence microscope. Comet assay Comet assays

(15) OCT4 and NANOG are involved in adrenocortical tumorigenesisArchives of Endocrinology and MetabolismMay 26, 2026Marcelo M. Cavalcanti, Mariana P. Halah, Letícia F. Leal, Fernanda B. Coeli-Lacchini, Carlos A. Scrideli, Carlos A. F. Molina, Jose A. Yunes, Silvia Brandalise, Ayrton C. Moreira, Fernando Ramalho, et al.counterstaining, cells were incubated with 4’, 6-diamidino-2-phenylindole (DAPi; Cell Signaling Technology, #4083, dilution: 1:25,000) for 2 minutes, washed in 1X PBS, and slides were set with Fluoromount (Sigma-Aldrich).

(16) GABA signaling in NG2 glia mediates empathy-like behavior under observational social defeatNature CommunicationsMay 25, 2026Yujin Jian, Shengyu Jin, Peng Liu, Xiaoli Zheng, Xiaoqi Hong, Yong Han, Alexey Semyanov, Shumin Duan, Xiaoping TongFluor 488 (1:500, Cell Signaling 4412 S). Nuclei were counterstained with DAPI (1:1000, Cell Signaling 4083S) for 15 min, and sections were mounted with Fluoromount™ Aqueous Mounting Medium (AQUA-MOUNT, REF 13800).

(17) Double-stranded RNA and ROS scavenging nanoplatform for modulating skin inflammationNature CommunicationsMay 13, 2026Lian Cui, Hengli Lu, Jiangluyi Cai, Ying Li, Zeyu Chen, Yiwei Zhang, Bingjie Li, Yuanyuan Wang, Nan Yang, Lingling Yao, et al.instructions. The sections were then mounted in ProLong Gold antifade reagent containing DAPI (Catalog #4083, Cell Signaling Technology) and visualized using a microscope (Leica). Mean fluorescence intensity (MFI)

(18) Age-Associated Impairment of Paneth Cells Driven by microRNA-152 Promotes Intestinal Epithelial Vulnerability to Pathological StressAging CellMay 13, 2026Bridgette Warner, Haonan Zhao, Hongxia Chen, Amy VanderStoep, Ana S. G. Cunningham, Dongyoon Yoo, Hee K. Chung, Christine Brantner, Jennifer Coleman, Joungil Choi, et al.,BD Biosciences # 610182 MUC2,Abcam # ab272692 Ki67,Abcam # ab15580 DAPI,Cell Signaling Technology # 4083S Anti‐Rabbit IgG (H + L),Invitrogen # A32731 Anti‐Mouse IgG (H + L),Invitrogen # A32727 Antibodies

(19) Dexmedetomidine Exerts Multi-level Effects to Ameliorate Alzheimer's Disease Pathology in the Adult Zebrafish BrainMol NeurobiolMay 5, 2026Dilek Nazli, Yusuf Kaan Poyraz, Kubilay Can, Dogac Ipekgil, Nilay Cakmak, Ebru Turhanlar-Sahin, Sevcan Hacoglu, Hale Aksu Erdost, Leyla Iyilikci, Gunes OzhanJackson ImmunoResearch Laboratories, PA, USA). Nuclear staining was performed with 4′,6-diamidino-2-phenylindole (1 µg/ml; DAPI; Cat# 4083S; Cell Signaling Technology, MA, USA). All primary antibodies used in this study were validated based

(20) OptoTAT reveals microtubule acetylation as a rapid trigger for GEF-H1-mediated cell migrationJ Cell BiolMay 4, 2026Abhijit Deb Deb Roy, Cristian Saez Gonzalez, Milda Stanislauskas, Farid Shahid, Eesha Yadav, Jalil Rezek, Takanari InoueA11057, A21058, A21096, A11032), Phalloidin (ThermoFisher A22286) and DAPI (Cell Signaling Technology, 4083S). After that, they were washed three times in PBS and the images were captured by microscopy. For HeLa cells

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DAPI

DAPI from Cell Signaling Technology

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DAPI from Cell Signaling Technology

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