DAPI from Cell Signaling Technology

Product Specs
ItemDAPI
CompanyCell Signaling Technology
Price $74.00 Inquire
Catalog Number4083S
Quantity1 mg
Research AreaRelated Products
ApplicationsIF-IC, FC-FP

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

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Website: http://www.cellsignal.com/

Website: Technical Support

(61) SIK2-mediated phosphorylation of GABARAPL2 facilitates autophagosome-lysosome fusion and rescues neurodegeneration in an Alzheimer's disease modelTransl NeurodegenerOctober 23, 2025Xiaoman Dai, Ziling Ye, Chen Wang, Yufei Huang, Yun Chen, Tianqing Han, Weijie Gao, Xin Wu, Jing Zhang, Xiaochun Chenapplied overnight at 4 °C. After washes, Alexa Fluor-conjugated secondary antibodies and DAPI (CST, #4083, Danvers, MA) were applied for 10 min at RT. Sections were mounted with ProLong Gold Antifade Mountant

(62) Tuning viscoelasticity of dynamic covalent hydrogels for human tissue modelingbioRxivOctober 22, 2025Narelli de Paiva de Paiva Narciso, Fotis Christakopoulos, Michelle S. Huang, Neil J. Baugh, Carla Huerta-López, Eliana X. Matos, Kristine P. Pashin, Andrew Spakowitz, Sarah C. HeilshornFluor 555 (1:500, Invitrogen, A-21422). 4’,6-diamidino-2-phenylindole (DAPI; 1μg/mL, Cell Signaling, 4083s) was included to stain for nuclei. Huang et al. (2025b) Secondary antibodies were incubated overnight

(63) Larp1 supports brain growth and spatial memory via post-transcriptional control of the translation machinerybioRxivOctober 14, 2025Mark S. Williams, Maegan J. Watson, Carson C. Thoreen800CW,926-32211,LI-COR Bio,WB 1:5000 Anti-Rabbit HRP Conjugate,7074S,Cell Signaling,WB 1:5000 DAPI,4083S,Cell Signaling,1 μg/mL Antibodies used in this study.

(64) Reproducible generation of Nipah virus pseudovirions with uniform incorporation of F and G surface glycoproteins for high-throughput neutralization assaysBMC MicrobiolOctober 8, 2025Geetu Rose Varghese, Vivek Vijay, Sreeja Sreedevi, Santhik S. Lupitha, Priya Prabhakaran, Sushama Aswathyraj, Anitha P. Moorkoth, Niyas K. Pulloor, Easwaran SreekumarCat. # 8889 S, CST, USA) respectively at 1:1000 dilution. DAPI (Cell Signalling Technology, Cat. # 4083 S, CST, MA, USA) (1 µg/ml) was used as the nuclear stain in all experiments. Production of NiV pseudovirions NiV

(65) Integration of Primary Murine Osteocytes for In Vitro Mechanobiology StudiesCurr ProtocOctober 8, 2025Kimberly Seaman, Chun-Yu Lin, Xin Song, Amel Sassi, William W. Du, Yu Sun, Burton Yang, Lidan Yousecondary antibody solution (see recipe)4',6‐diamidino‐2‐phenylindole (DAPI) (Cell Signaling Technology, 4083) Biosafety cabinet (Thermo Scientific, Model 1355 Class A2)Aspirator (Gast, 0523‐101Q‐G180DX)Cell culture

(66) Targeting G1-S-checkpoint-compromised cancers with cyclin A/B RxL inhibitorsNatureOctober 1, 2025Singh S, Gleason CE, Fang M, Laimon YN, Khivansara V, Xie S, Durmaz YT, Sarkar A, Ngo K, Savla V, et al.in PBS containing 0.5% BSA, centrifuged at 400g and then counterstained with DAPI) (Cell Signaling, 4083) for 15 min at room temperature. FACS analysis was then performed to determine the percentage of positive

(67) Cholesterol 25‐Hydroxylase Enhances Myeloid‐Derived Suppressor Cell (MDSC) Immunosuppression via the Stimulator of Interferon Genes (STING)‐Tank‐Binding Kinase 1 (TBK1)‐Receptor‐Interacting Protein Kinase 3 (RIPK3) Pathway in Colorectal CancerMedComm (2020)October 1, 2025Dongqin Zhou, Yu Chen, Xudong Liu, Juan He, Luyao Shen, Yongpeng He, Jiangang Zhang, Yu Zhou, Nan Zhang, Yanquan Xu, et al.sections were stained with 4′,6‐diamidino‐2′‐phenylindole (DAPI; Cell Signaling Technology, Catalogue No. 4083S) antifade reagent and visualized using a Leica fluorescence confocal microscope. For frozen section

(68) AARS1-mediated lactylation of H3K18 and STAT1 promotes ferroptosis in diabetic nephropathyCell Death & DifferentiationSeptember 23, 2025Jia Hong, Hongjiao Xu, Lang Yu, Zhuang Yu, Xiangyuan Chen, Zhipeng Meng, Jiali Zhu, Jinbao Li, Minmin ZhuChina) fusion proteins were used in this study. For the IF assay, the nuclei were stained with DAPI (4083S; Cell Signaling Technology, MA, USA), and the endoplasmic reticulum was stained with ER-Tracker (C1041S,

(69) Zinc Promotes Mitochondrial Health Through PGC-1alpha Enhancing Bacterial Clearance in Macrophages Infected with Mycobacterium avium ComplexInternational Journal of Molecular SciencesSeptember 23, 2025Ruxana T. Sadikot, Prabagaran Narayanasamy, Zhihong Yuan, Deandra Smith, Daren L. KnoellT668), MitoSOX Red (Invitrogen, M36007) 4’, 6-diamidino-2-phenylindole, dihydrochloride (DAPI) (CST, 4083), Hoechst 33342 (CST, 4082). Unless otherwise specified, reagents and chemicals were obtained through

(70) Epigenome and transcriptome changes in KMT2D-related Kabuki syndrome Type 1 iPSCs, neuronal progenitors and cortical neuronsPLOS GeneticsSeptember 19, 2025Sara Cuvertino, Evgenii Martirosian, Kedar Bhosale, Peiwen Cheng, Terence Garner, Ian J. Donaldson, Adam Jackson, Adam Stevens, Andrew D. Sharrocks, Susan J. Kimber, et al.at room temperature in the dark. After washing three times with PBS, samples were stained with DAPI (4083S, Cell signalling technology) for 5 minutes. Finally, samples were washed with PBS. Pictures were taken

(71) Biogenic Synthesis of Gold Nanoparticles Using Scabiosa palaestina Extract: Characterization, Anticancer and Antioxidant ActivitiesNanomaterials (Basel)September 4, 2025Heba Hellany, Adnan Badran, Ghosoon Albahri, Nadine Kafrouny, Riham El Kurdi, Marc Maresca, Digambara Patra, Elias Baydounmorphological changes, cells were stained with 4′,6-diamidino-2-phenylindole (DAPI) (Cell Signaling #4083, Massachusetts, USA). For this, cells were seeded in 12-well plates and treated with the indicated concentrations

(72) Autism-related proteins form a complex to maintain the striatal asymmetry in miceCell ResearchSeptember 2, 2025Yisheng Jiang, Feipeng Zhu, Jie Zhong, Xiaomei Sun, Yuting Yuan, Shuo Wang, Haiyang Chen, Zhiheng Xuappropriate fluorescent secondary antibodies (1:2000; Invitrogen) and DAPI (Cell Signaling Technology, Cat# 4083s) at RT for 1 h, followed by PBST washing for 10 min three times. Fluorescent images were captured using

(73) Mandragora autumnalis: Phytochemical Composition, Antioxidant and Anti-Cancerous Bioactivities on Triple-Negative Breast Cancer CellsInternational Journal of Molecular SciencesSeptember 1, 2025Ghosoon Albahri, Adnan Badran, Heba Hellany, Serine Baydoun, Rola Abdallah, Mohamad Alame, Akram Hijazi, Marc Maresca, Elias Baydounμg/mL). Following the manufacturer’s instructions, the cells were stained with DAPI (Cell Signaling #4083), fixed with 4% formaldehyde, and then fluorescence microscopy was used to visualize the apoptotic morphological

(74) Mechanosensitive Channels Mediate Pancreatic β Cells Reactive Oxygen Species Formation and Downregulation of Essential Genes During Therapeutic Ultrasound TreatmentJournal of Ultrasound in MedicineSeptember 1, 2025Mallory Brayer, Vesna Zderic, Aleksandar Jeremicat room temperature for 1 hour. Cells were then incubated with DAPI (Cell Signaling catalog number 4083) in PBS for 20 minutes prior to imaging. Images were taken using a Zeiss LSCM-800 confocal microscope

(75) Reprogramming of Fatty Acid Metabolism via PPARα‐Orchestrated FADS2 in Keratinocytes Modulates Skin Inflammation in PsoriasisAdvanced ScienceAugust 29, 2025Jiangluyi Cai, Xue Zhou, Yu Zhuang, Lian Cui, Rui Ma, Youdong Chen, Nan Yang, Qianyu Chen, Yuanyuan Wang, Peiyao Zhu, et al.the sections according to the manufacturer's instructions. The sections were then stained with DAPI (#4083, Cell Signaling Technology). Images were captured using an Olympus fluorescence microscope. Signals

(76) Green Synthesis of Gold Nanoparticles Using Mandragora autumnalis: Characterization and Evaluation of Its Antioxidant and Anticancer BioactivitiesPharmaceuticals (Basel)August 29, 2025Ghosoon Albahri, Adnan Badran, Heba Hellany, Nadine Kafrouny, Riham El Kurdi, Mohamad Alame, Akram Hijazi, Marc Maresca, Digambara Patra, Elias Baydounmorphology were identified using 4′, 6-diamidino-2-phenylindole, dihydrochloride (DAPI) (Cell Signaling #4083) staining. For 24 h, cells were cultured in 12-well plates with or without the specified MAE-AuNP concentrations.

(77) Development of NAFLD‐Specific Human Liver Organoid Models on a Microengineered Array Chip for Semaglutide Efficacy EvaluationCell ProliferationAugust 27, 2025Xiao‐yan You, Xiang‐yang Li, Hui Wang, Guo‐ping Zhao4412s/8889s/4408s; Beyotime, A0502) for 1 h at 25°C. Nuclei were counterstained with 1 μg/mL DAPI (Cell Signalling, 4083) for 10 min. Slides were mounted in 50% glycerol/DPBS (v/v) and imaged using a DM5000B fluorescence microscope

(78) Ageing‐Dependent Thyroid Hormone Receptor α Reduction Activates IP3R1‐Meditated Ca2+ Transfer in MAM and Exacerbates Skeletal Muscle Atrophy in MiceCell ProliferationAugust 24, 2025Runqing Shi, Yusheng Zhang, Gong Chen, Jiru Zhang, Jing Liu, Hao Zhu, Minne Sun, Yu Duanwas performed the following day. The nuclei were stained with 4,6‐diamidino‐2‐phenylindole (DAPI, #4083, CST). 2.12 Mitochondrial Fluorescent Staining To detect MMP, ROS, Ca2+, and mitochondria, respectively,

(79) NEDD4L-Mediated Ubiquitination of GPX4 Exacerbates Doxorubicin-Induced CardiotoxicityInternational Journal of Molecular SciencesAugust 23, 2025Jiaxing Ke, Lingjia Li, Shuling Chen, Chenxin Liao, Feng Peng, Dajun Chai, Jinxiu LinImmunoway (Plano, TX, USA). Antibodies against ubiquitin (Ub, 43124), Flag (70586), Myc (2276), and DAPI (4083S) were purchased from Cell Signaling Technology (Danvers, MA, USA). Antibodies against K48-linkage-specific

(80) miR-9-5p alleviates the development of abdominal aortic aneurysm by regulating the differentiation of CD4+IL-10+T cells via targeting the crosstalk between Nrf2 and NF-κB signaling pathwaysTurkish Journal of BiologyAugust 8, 2025HONGFU LIU, JINYI ZHANG, LUBIN LI, BENXIANG YU, CHUNLEI ZHANG, WENQIANG NIU, YAWEN CHENG, HENGYANG DONG, YUKUN ZHANG, XINLIN LUO, et al.T cells, and consequently clinically reduce AAA lesions. 2. Materials and methods 2.1. Reagents DAPI(#4083) and antibodies against Histon H3 (# 4499 ), Nrf2 (#12721), NF-κB (#8242), β-actin (# 4970 ), HO-1 (#43966),

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DAPI

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