DAPI from Cell Signaling Technology

Product Specs
ItemDAPI
CompanyCell Signaling Technology
Price $74.00 Inquire
Catalog Number4083S
Quantity1 mg
Research AreaRelated Products
ApplicationsIF-IC, FC-FP

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

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Website: http://www.cellsignal.com/

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(61) Human pluripotent stem cell-derived skin organoids enabled pathophysiological model of Mycobacterium tuberculosis infectionNat CommunDecember 2, 2025Liang Yue, Yating Liang, Pengfei Zhong, Leiming Fang, Zhimin Yun, Qisheng Su, Chaofan Li, Meida Xiang, Jie Zhang, Jiru Wang, et al.room temperature protected from light. After repeating the previous washing steps, DAPI staining (#4083, Cell Signaling) was added in DPBS for 15 min at room temperature in the dark. Finally, samples were

(62) Thymosin β4 stabilizes hypoxia induced brain microvascular endothelial cell dysfunction through S1PR1 dependent mechanismsSci RepDecember 1, 2025William G. Stewart, Christina D. Hejl, Rakeshwar S. Guleria, Sudhiranjan Guptawere captured by Leica DMi8 Imaging system. Anti-S1PR1 (cat. no. 63335, lot no.1) and DAPI (cat. no. 4083) were purchased from Cell Signaling Technology, Inc., USA. Western blotting The protein lysates were

(63) Follicle-like niches outside the cortex? 3D phase-contrast µCT revealed medullary B cell nodules in mucosa-draining lymph nodesFrontiers in ImmunologyNovember 19, 2025Paul Schütz, Florian L. Schwarzenberg, Lennert J. Weber, Jörg U. Hammel, Bente Siebels, Paula Nissen, Nenya Leising, Katja J. Jarick, Bernd Walkenfort, Sarah C. Irvine, et al.3x 10 min in 0.1% PBST and incubated with DAPI as a nuclear counterstain (Cell Signaling, Cat. No.: 4083S, Cambridge, UK) 1:1000 in PBS for 2 hours. For stainings using unconjugated (primary) antibodies, secondary

(64) KIF11 variants in familial exudative vitreoretinopathy leading to mTORC1 overactivation and impaired cell cycle progressionHuman GenomicsNovember 19, 2025Min Liu, Shengliu Pan, Erkuan Dai, Rulian ZhaoTechnology), rabbit anti-p21 (10355-1-AP, Proteintech), rabbit anti-CDK1 (19532-1-AP, Proteintech), DAPI (4083, Cell Signaling Technology), Hoechst (CM03826, Proteintech), anti-mouse IgG (H + L), Alexa Fluor Plus

(65) Targeting plasticity in the pyrimidine synthesis pathway potentiates macrophage-mediated phagocytosis in pancreatic cancer modelsJ Clin InvestNovember 17, 2025Jie Zhao, Xinghao Li, Xinyu Li, Pengfei Ren, Yilan Wu, Hao Gong, Lijian Wu, Junran Huang, Saisai Wang, Ziwei Guo, et al.0.1 mg/mL APC-F4/80 (BioLegend, catalog 123116) and 1 mg/mL DAPI (Cell Signaling Technology, catalog 4083S) in 50 μL FACS buffer for 20 minutes at room temperature. The cells were then resuspended in 100 μL

(66) Pinostrobin Inhibits Nuclear Factor-Kappa B Signaling and Production of Inflammatory Cytokines and Chemokines in Human MacrophagesNutrientsNovember 17, 2025Phatcharaporn Budluang, Saranyapin Potikanond, Nitwara Wikan, Wutigri Nimlamoolgoat anti-mouse IgG conjugated with Alexa488 (both from Thermo Fisher Scientific) and DAPI (Cat No. 4083) (Cell Signaling Technology) for detecting the cell nuclei. The coverslips were mounted onto a fluorescent microscope

(67) Barium titanate piezoelectric nanoparticles induce M1 polarization in mouse macrophages via ultrasound in vitroScientific ReportsNovember 12, 2025Timothy Connolly, Camille Johnson, Allison Chen, Krzysztof Kempa, Dylan Hatt, Michael J. Naughtonin PBS. A 5 μg ml−1 solution of 4′,6-diamidino-2-phenylindole (DAPI; Cell Signaling Technology, no. 4083) was then added to the cells for 10 min. The DAPI stain was removed and cells were washed 3 times with

(68) TFNAs induce autophagy via activation of the nonclassical cGAS-STING signalling pathway to attenuate acute lung injury in miceBMC pulmonary medicineNovember 7, 2025Wei C, Liu Y, Pan B, Zhang J, Chen J, Ling P, Xiang Y, Zhu S, Sun Y.= 1:200). After washing with PBS, the nuclei were labelled with 4,6-diamino-2-phenyl-indole (DAPI; 4083, CST, US; DAPI/PBS = 1:500). Images were acquired via a fluorescence microscope (Leica, German). Flow cytometry

(69) ISG20 Restricts BK Polyomavirus Infection and Engages in Reciprocal Regulation with Viral Large T AntigenMicroorganismsNovember 6, 2025Yumin Hou, Chunlan Hu, Yejing Shi, Xiaohui Zhou, Tongyu Zhu, Nannan Wuanti-mouse antibody (Invitrogen, #A10037). Nuclei were counterstained with DAPI (Cell Signaling Technology, #4083) for 10 min before mounting. Images were acquired using a Tissue FAXS 200 flow-type tissue cytometer

(70) Methotrexate alleviates chronic inflammation in a Drosophila modelJournal of Cell ScienceNovember 1, 2025Dushyant K. Gautam, Willem Buys, Zeeshan Ahmad, Ravi K. Gutti, Indira Paddibhatlathree washes, the samples were counterstained for 15 min at room temperature with the nuclear dye DAPI (4083, 2 µg/ml, CST) or Hoechst 33342 (4082, 2.5 µg/ml, CST) as the final step for staining procedure. Final

(71) TH1 effector CD4 T cells rely on IFN-γ production to induce alopecia areataSci AdvOctober 31, 2025Samuel J. Connell, Sydney Crotts, Ryan Reis, Maddison M. Lensing, Payton Kahl, Nicholas Henderson, Otgonzaya Ayush, Zhaowen Zhu, Luana S. Ortolan, Audrey C. Ko, et al.of nuclei, sections were stained using 4′,6-diamidino-2-phenylindole (DAPI; 1 μg/ml; Cell Signaling, 4083) for 5 min at room temperature. Antifade mountant with DAPI (Thermo Fisher Scientific, P36962) was used

(72) SIK2-mediated phosphorylation of GABARAPL2 facilitates autophagosome-lysosome fusion and rescues neurodegeneration in an Alzheimer's disease modelTransl NeurodegenerOctober 23, 2025Xiaoman Dai, Ziling Ye, Chen Wang, Yufei Huang, Yun Chen, Tianqing Han, Weijie Gao, Xin Wu, Jing Zhang, Xiaochun Chenapplied overnight at 4 °C. After washes, Alexa Fluor-conjugated secondary antibodies and DAPI (CST, #4083, Danvers, MA) were applied for 10 min at RT. Sections were mounted with ProLong Gold Antifade Mountant

(73) Tuning viscoelasticity of dynamic covalent hydrogels for human tissue modelingbioRxivOctober 22, 2025Narelli de Paiva de Paiva Narciso, Fotis Christakopoulos, Michelle S. Huang, Neil J. Baugh, Carla Huerta-López, Eliana X. Matos, Kristine P. Pashin, Andrew Spakowitz, Sarah C. HeilshornFluor 555 (1:500, Invitrogen, A-21422). 4’,6-diamidino-2-phenylindole (DAPI; 1μg/mL, Cell Signaling, 4083s) was included to stain for nuclei. Huang et al. (2025b) Secondary antibodies were incubated overnight

(74) Larp1 supports brain growth and spatial memory via post-transcriptional control of the translation machinerybioRxivOctober 14, 2025Mark S. Williams, Maegan J. Watson, Carson C. Thoreen800CW,926-32211,LI-COR Bio,WB 1:5000 Anti-Rabbit HRP Conjugate,7074S,Cell Signaling,WB 1:5000 DAPI,4083S,Cell Signaling,1 μg/mL Antibodies used in this study.

(75) Integration of Primary Murine Osteocytes for In Vitro Mechanobiology StudiesCurr ProtocOctober 8, 2025Kimberly Seaman, Chun-Yu Lin, Xin Song, Amel Sassi, William W. Du, Yu Sun, Burton Yang, Lidan Yousecondary antibody solution (see recipe)4',6‐diamidino‐2‐phenylindole (DAPI) (Cell Signaling Technology, 4083) Biosafety cabinet (Thermo Scientific, Model 1355 Class A2)Aspirator (Gast, 0523‐101Q‐G180DX)Cell culture

(76) Reproducible generation of Nipah virus pseudovirions with uniform incorporation of F and G surface glycoproteins for high-throughput neutralization assaysBMC MicrobiolOctober 8, 2025Geetu Rose Varghese, Vivek Vijay, Sreeja Sreedevi, Santhik S. Lupitha, Priya Prabhakaran, Sushama Aswathyraj, Anitha P. Moorkoth, Niyas K. Pulloor, Easwaran SreekumarCat. # 8889 S, CST, USA) respectively at 1:1000 dilution. DAPI (Cell Signalling Technology, Cat. # 4083 S, CST, MA, USA) (1 µg/ml) was used as the nuclear stain in all experiments. Production of NiV pseudovirions NiV

(77) Cholesterol 25‐Hydroxylase Enhances Myeloid‐Derived Suppressor Cell (MDSC) Immunosuppression via the Stimulator of Interferon Genes (STING)‐Tank‐Binding Kinase 1 (TBK1)‐Receptor‐Interacting Protein Kinase 3 (RIPK3) Pathway in Colorectal CancerMedComm (2020)October 1, 2025Dongqin Zhou, Yu Chen, Xudong Liu, Juan He, Luyao Shen, Yongpeng He, Jiangang Zhang, Yu Zhou, Nan Zhang, Yanquan Xu, et al.sections were stained with 4′,6‐diamidino‐2′‐phenylindole (DAPI; Cell Signaling Technology, Catalogue No. 4083S) antifade reagent and visualized using a Leica fluorescence confocal microscope. For frozen section

(78) Targeting G1-S-checkpoint-compromised cancers with cyclin A/B RxL inhibitorsNatureOctober 1, 2025Singh S, Gleason CE, Fang M, Laimon YN, Khivansara V, Xie S, Durmaz YT, Sarkar A, Ngo K, Savla V, et al.in PBS containing 0.5% BSA, centrifuged at 400g and then counterstained with DAPI) (Cell Signaling, 4083) for 15 min at room temperature. FACS analysis was then performed to determine the percentage of positive

(79) Zinc Promotes Mitochondrial Health Through PGC-1alpha Enhancing Bacterial Clearance in Macrophages Infected with Mycobacterium avium ComplexInternational Journal of Molecular SciencesSeptember 23, 2025Ruxana T. Sadikot, Prabagaran Narayanasamy, Zhihong Yuan, Deandra Smith, Daren L. KnoellT668), MitoSOX Red (Invitrogen, M36007) 4’, 6-diamidino-2-phenylindole, dihydrochloride (DAPI) (CST, 4083), Hoechst 33342 (CST, 4082). Unless otherwise specified, reagents and chemicals were obtained through

(80) AARS1-mediated lactylation of H3K18 and STAT1 promotes ferroptosis in diabetic nephropathyCell Death & DifferentiationSeptember 23, 2025Jia Hong, Hongjiao Xu, Lang Yu, Zhuang Yu, Xiangyuan Chen, Zhipeng Meng, Jiali Zhu, Jinbao Li, Minmin ZhuChina) fusion proteins were used in this study. For the IF assay, the nuclei were stained with DAPI (4083S; Cell Signaling Technology, MA, USA), and the endoplasmic reticulum was stained with ER-Tracker (C1041S,

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DAPI

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